The epithelial-to mesenchymal transition: methods and protocols

This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plastici...

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Bibliographische Detailangaben
Weitere Verfasser: Campbell, Kyra (HerausgeberIn), Theveneau, Eric (HerausgeberIn)
Format: Buch
Sprache:English
Veröffentlicht: New York, NY Humana Press [2021]
Schriftenreihe:Methods in molecular biology 2179
Schlagworte:
Zusammenfassung:This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards
Beschreibung:This volume details a comprehensive range of methods for imaging epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards
Part I: Perspectives on the Past and Future of EMT Research; 1. A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition ; Erica J. Hutchins and Marianne E. Bronner; ; 2. Perspective on Epithelial Mesenchymal Transitions in Embryos; David R. McClay; ; 3. EMT, One of Many Morphological Transitions in Cellular Phase Space ; Denise J. Montell; ; 4. Are you Interested or Afraid of Working on EMT? ; M. Angela Nieto; ; 5. Partial EMT/MET: An Army of One; Sofiane Hamidi, Hiroki Nagai, and Guojun Sheng; ; 6. EMT; An Update; Jean Paul Thiery; ; Part II: Review on Mesenchymal-to-Epithelial Transitions; 7. Mesenchymal to Epithelial Transitions in Development and Cancer ; John-Poul Ng-Blichfeldt and Katja Röper; ; Part III: Methods to Study EMT in In Vivo Systems; 8. Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos; Lingkun Gu and Mo Weng; ; 9.
- Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration; Zain Alhashem, Macarena Alvarez-Garcillan Portillo, Ravinand Htun, Anton Gauert, Luis Briones Montecinos, Steffen Härtel, and Claudia Linker; ; 10. Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo; Mary Cathleen McKinney and Paul M. Kulesa; ; 11. Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors; Sofia Golenkina, Rosemary Manhire-Heath, and Michael J. Murray; ; 12. Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging; Wallis Nahaboo, Bechara Saykali, Navrita Mathiah, and Isabelle Migeotte; ; 13. Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo ; Joshua Greig and Natalia A. Bulgakova; ; 14.
- Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster ; Jamie Adams, Andreu Casali, and Kyra Campbell; ; Part IV: In Vivo Imaging of MET; 15. 4D Live-Imaging and Analysis of Chick Embryo Somites; Gi Fay Mok, James McColl, and Andrea Münsterberg; ; 16. In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation; Elena Gonzalez-Gobartt, Guillaume Allio, Bertrand Bénazéraf, and Elisa Martí; ; 17. Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos ; Gordana Scepanovic, Alexandru Florea, and Rodrigo Fernandez-Gonzalez; ; Part V: Ex Vivo/InVitro Methods to Study EMT/MEt; 18. Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering; Minerva Bosch-Fortea and Fernando Martín-Belmonte; ; 19.
- CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay ; Sefora Conti, Takuya Kato, Danielle Park, Erik Sahai, Xavier Trepat, Anna Labernadie ; ; 20. Using Xenopus Neural Crest Explants to Study; Epithelial-mesenchymal Transition; Nadège Gouignard, Christian Rouvière, and Eric Theveneau; ; 21. Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition; Hye Young Kim and Lance A. Davidson; ; 22. Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition; Helena Canever, Pietro Salvatore Carollo, Romain Fleurisson, Philippe P. Girard, and Nicolas Borghi; ; Part VI: Methods to Study Epithelial- Mesenhymal Plasticity at the Single Cell, Subcellular, and Molecular Level; 23. Methodologies for Following EMT in vivo at Single Cell Resolution; Abdull J. Massri, Geoffrey R. Schiebinger, Alejandro Berrio, Lingyu Wang, Gregory A. Wray, and David R. McClay; ; 24. Isolation and Identification of EMT Subtypes; Robert J.
- Norgard and Ben Z. Stanger; ; 25. Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer; Sugandha Bhatia, Erik W Thompson, and Jennifer H Gunter; ; 26. Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells; Cecile Davaine, Eva Hadadi, William Taylor, Annelise Bennaceur-Griscelli, and Hervé Acloque; ; 27. Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling; Noémie Kempf, Fatima Moutahir, Isabelle Goiffon, Sylvain Cantaloube, Kerstin Bystricky, and Anne-Claire Lavigne; ; 28. Mathematical Modeling of Plasticity and Heterogeneity in EMT; Shubham Tripathi, Jianhua Xing, Herbert Levine, and Mohit Kumar Jolly; ; 29. Control of Cell Migration Using Optogenetics; Leo Valon and Simon de Beco;
Beschreibung:xv, 429 Seiten Illustrationen, Diagramme 1034 grams
ISBN:9781071607787

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