Erythropoiesis: methods and protocols
This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters,...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2018
|
| Schriftenreihe: | Methods in Molecular Biology
1698 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Erythropoiesis: Methods and Protocols provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward |
| Beschreibung: | 1 Online-Ressource (XI, 298 Seiten) Illustrationen |
| ISBN: | 9781493974283 |
| DOI: | 10.1007/978-1-4939-7428-3 |
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| indexdate | 2024-07-10T08:05:37Z |
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| isbn | 9781493974283 |
| language | English |
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| spelling | Erythropoiesis methods and protocols edited by Joyce A. Lloyd New York, NY Springer New York 2018 1 Online-Ressource (XI, 298 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1698 This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Erythropoiesis: Methods and Protocols provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward Life Sciences Cell Biology Life sciences Cell biology Lloyd, Joyce A. edt Erscheint auch als Druck-Ausgabe 9781493974276 https://doi.org/10.1007/978-1-4939-7428-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Erythropoiesis methods and protocols Life Sciences Cell Biology Life sciences Cell biology |
| title | Erythropoiesis methods and protocols |
| title_auth | Erythropoiesis methods and protocols |
| title_exact_search | Erythropoiesis methods and protocols |
| title_full | Erythropoiesis methods and protocols edited by Joyce A. Lloyd |
| title_fullStr | Erythropoiesis methods and protocols edited by Joyce A. Lloyd |
| title_full_unstemmed | Erythropoiesis methods and protocols edited by Joyce A. Lloyd |
| title_short | Erythropoiesis |
| title_sort | erythropoiesis methods and protocols |
| title_sub | methods and protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7428-3 |
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