Xenopus Protocols: Cell Biology and Signal Transduction
Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Tra...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Schriftenreihe: | Methods in Molecular Biology™
322 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses |
| Beschreibung: | 1 Online-Ressource (XVI, 489 p) |
| ISBN: | 9781597450003 |
| DOI: | 10.1007/978-1-59745-000-3 |
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| 520 | |a Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses | ||
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Datensatz im Suchindex
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| spelling | Xenopus Protocols Cell Biology and Signal Transduction edited by X. Johné Liu Totowa, NJ Humana Press 2006 1 Online-Ressource (XVI, 489 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 322 Although Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses Life Sciences Biochemistry, general Developmental Biology Life sciences Biochemistry Developmental biology Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Krallenfrosch (DE-588)4165479-1 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Krallenfrosch (DE-588)4165479-1 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s b DE-604 Liu, X. Johné edt Erscheint auch als Druck-Ausgabe 9781588293626 https://doi.org/10.1007/978-1-59745-000-3 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Xenopus Protocols Cell Biology and Signal Transduction Life Sciences Biochemistry, general Developmental Biology Life sciences Biochemistry Developmental biology Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Krallenfrosch (DE-588)4165479-1 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 (DE-588)4165479-1 (DE-588)4143413-4 |
| title | Xenopus Protocols Cell Biology and Signal Transduction |
| title_auth | Xenopus Protocols Cell Biology and Signal Transduction |
| title_exact_search | Xenopus Protocols Cell Biology and Signal Transduction |
| title_full | Xenopus Protocols Cell Biology and Signal Transduction edited by X. Johné Liu |
| title_fullStr | Xenopus Protocols Cell Biology and Signal Transduction edited by X. Johné Liu |
| title_full_unstemmed | Xenopus Protocols Cell Biology and Signal Transduction edited by X. Johné Liu |
| title_short | Xenopus Protocols |
| title_sort | xenopus protocols cell biology and signal transduction |
| title_sub | Cell Biology and Signal Transduction |
| topic | Life Sciences Biochemistry, general Developmental Biology Life sciences Biochemistry Developmental biology Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Krallenfrosch (DE-588)4165479-1 gnd |
| topic_facet | Life Sciences Biochemistry, general Developmental Biology Life sciences Biochemistry Developmental biology Molekularbiologie Methode Krallenfrosch Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-59745-000-3 |
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