Xenopus protocols: cell biology and signal transduction
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Schriftenreihe: | Methods in molecular biology
322 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XV, 489 S. Ill., graph. Darst. |
| ISBN: | 1588293629 |
Internformat
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| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a XV, 489 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a cellular biology |2 cabt | |
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| 650 | 7 | |a genetic analysis |2 cabt | |
| 650 | 7 | |a laboratory methods |2 cabt | |
| 650 | 4 | |a Cellular signal transduction |v Laboratory manuals | |
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| 650 | 4 | |a Signal Transduction |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804135459343826944 |
|---|---|
| adam_text | Contents
Preface
Contributors
1
Steven L. Klein,
Lynn M. Schriml, Amy K.
and John D. McPherson
2
Melissa
3
X. Shawn Liu and X.
4
lean-François
5
Xavier
and Michel
6
by Confocal Immunofluorescence Microscopy
Bret E. Becker and
7
for Xenopus Oocyte Research
Angela M. Prouty, fun Wu, Da-Ting Lin, Patricia
and fames D. Lechleiter
8
Sheila L. Dargan,
9
Derived From Xenopus Eggs
Antonin
10
Into Xenopus Oocytes
Daniele
11
Kyong Hwa Moon, Xinliang Zhao, and Yi-Tao Yu
vii
viii
12
Regulation in Xenopus Oocytes and Tadpoles
David Stewart, Akihiro
13
María Piqué, José
14
Convertases
Kathleen I. J. Shennan
15
Protein Refolding Assay
JohnJ. Heikkila,
16
Anna Castro, Suzanne
and
17
Molecules and Mechanisms
Reiner Peters
18
in Nucleocytoplasmic Transport Studies
Reiner Peters
19
by Electron and Atomic Force Microscopy
Bohumil
and
20
Using Xenopus Egg Extracts
Rene C. Chan and Douglass J. Forbes
21
Transport Studies
Nelly
22
Using Oocyte Patches
Catherine F. Morris, Peter F. Juranka, Wei Lin, Terence J. Morris,
and
23
Targets of Drugs and Pesticides
Steven David Buckingham, Luanda
Contents ix
24 Microtransplantation
From Cells to Xenopus Oocyte Membranes:
New Procedure for Ion Channel Studies
Ricardo
25
Egg Extracts in Semi-Intact MDCK Cells
Fumi
26
Andrea V. Gamarnik and Raul
27
Reconstitution
Paula Deming and Sally Kornbluth
28
on Membrane/Lipid Raft Functions and Proteomics
Ken-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov,
Tetsushi Iwasaki, Kazuyoshi Yonezawa, and Yasuo Fukami
29
of Xenopus laevis Cytoplasm for Subcellular Biochemical Assays
Christopher E. Sims, Veronica Luzzi, and Nancy L
30
Using Expressed Substrate in Live Cells
¡ing Wang and X.
31
Richard
32
Keita Ohsumi, Tomomi M. Yamamoto, and
33
and Chromosome Condensation in Xenopus Egg Extracts
Thomas J. Maresca and Rebecca Heald
Index
Although Xenopus oocytes are very hardy and robust in terms of experimentation and
offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cyto¬
plasm and
Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and
cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute
biological and cellular processes. These readily reproducible methods take advantage of the
oocyte s impressive protein abundance (for example,
oocyte), its striking protein translation capacity
breathtaking possibilities for the assembly of infectious viral particles by single-cell injection
of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell
biology and signal transduction and cover all the major uses of oocytes/extracts as experi¬
mental models. The protocols follow the successful Methods in Molecular Biology™series
format, each offering step-by-step laboratory instructions, an introduction outlining the prin¬
ciples behind the technique, lists of the necessary equipment and reagents, and tips on
troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal
Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as
well as seasoned investigators interested in developing novel models and uses.
FEATURES
Readily reproducible methods for using
Xenopus oocytes and oocyte/egg extracts
Coverage of all the major uses of oocytes/
extracts as experimental models
Focus on the versatility of frog oocytes and
egg extracts in cell biology and signal
transduction
•
results
•
shooting and avoiding known pitfalls
|
| adam_txt |
Contents
Preface
Contributors
1
Steven L. Klein,
Lynn M. Schriml, Amy K.
and John D. McPherson
2
Melissa
3
X. Shawn Liu and X.
4
lean-François
5
Xavier
and Michel
6
by Confocal Immunofluorescence Microscopy
Bret E. Becker and
7
for Xenopus Oocyte Research
Angela M. Prouty, fun Wu, Da-Ting Lin, Patricia
and fames D. Lechleiter
8
Sheila L. Dargan,
9
Derived From Xenopus Eggs
Antonin
10
Into Xenopus Oocytes
Daniele
11
Kyong Hwa Moon, Xinliang Zhao, and Yi-Tao Yu
vii
viii
12
Regulation in Xenopus Oocytes and Tadpoles
David Stewart, Akihiro
13
María Piqué, José
14
Convertases
Kathleen I. J. Shennan
15
Protein Refolding Assay
JohnJ. Heikkila,
16
Anna Castro, Suzanne
and
17
Molecules and Mechanisms
Reiner Peters
18
in Nucleocytoplasmic Transport Studies
Reiner Peters
19
by Electron and Atomic Force Microscopy
Bohumil
and
20
Using Xenopus Egg Extracts
Rene C. Chan and Douglass J. Forbes
21
Transport Studies
Nelly
22
Using Oocyte Patches
Catherine F. Morris, Peter F. Juranka, Wei Lin, Terence J. Morris,
and
23
Targets of Drugs and Pesticides
Steven David Buckingham, Luanda
Contents ix
24 Microtransplantation
From Cells to Xenopus Oocyte Membranes:
New Procedure for Ion Channel Studies
Ricardo
25
Egg Extracts in Semi-Intact MDCK Cells
Fumi
26
Andrea V. Gamarnik and Raul
27
Reconstitution
Paula Deming and Sally Kornbluth
28
on Membrane/Lipid Raft Functions and Proteomics
Ken-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov,
Tetsushi Iwasaki, Kazuyoshi Yonezawa, and Yasuo Fukami
29
of Xenopus laevis Cytoplasm for Subcellular Biochemical Assays
Christopher E. Sims, Veronica Luzzi, and Nancy L
30
Using Expressed Substrate in Live Cells
¡ing Wang and X.
31
Richard
32
Keita Ohsumi, Tomomi M. Yamamoto, and
33
and Chromosome Condensation in Xenopus Egg Extracts
Thomas J. Maresca and Rebecca Heald
Index
Although Xenopus oocytes are very hardy and robust in terms of experimentation and
offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cyto¬
plasm and
Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and
cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute
biological and cellular processes. These readily reproducible methods take advantage of the
oocyte's impressive protein abundance (for example,
oocyte), its striking protein translation capacity
breathtaking possibilities for the assembly of infectious viral particles by single-cell injection
of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell
biology and signal transduction and cover all the major uses of oocytes/extracts as experi¬
mental models. The protocols follow the successful Methods in Molecular Biology™series
format, each offering step-by-step laboratory instructions, an introduction outlining the prin¬
ciples behind the technique, lists of the necessary equipment and reagents, and tips on
troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal
Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as
well as seasoned investigators interested in developing novel models and uses.
FEATURES
Readily reproducible methods for using
Xenopus oocytes and oocyte/egg extracts
Coverage of all the major uses of oocytes/
extracts as experimental models
Focus on the versatility of frog oocytes and
egg extracts in cell biology and signal
transduction
•
results
•
shooting and avoiding known pitfalls |
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| series | Methods in molecular biology |
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| spelling | Xenopus protocols cell biology and signal transduction ed. by X. Johné Liu Totowa, NJ Humana Press 2006 XV, 489 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 322 Xenopus cabt cellular biology cabt oocytes cabt genetic analysis cabt laboratory methods cabt Cellular signal transduction Laboratory manuals Oocytes cytology Laboratory Manuals Signal Transduction Laboratory Manuals Xenopus laevis Cytology Laboratory manuals Xenopus laevis Eggs Laboratory manuals Xenopus embryology Laboratory Manuals Krallenfrosch (DE-588)4165479-1 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Krallenfrosch (DE-588)4165479-1 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s b DE-604 Liu, X. Johné (DE-588)1159173877 edt Methods in molecular biology 322 (DE-604)BV035362695 322 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866881&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866881&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Xenopus protocols cell biology and signal transduction Methods in molecular biology Xenopus cabt cellular biology cabt oocytes cabt genetic analysis cabt laboratory methods cabt Cellular signal transduction Laboratory manuals Oocytes cytology Laboratory Manuals Signal Transduction Laboratory Manuals Xenopus laevis Cytology Laboratory manuals Xenopus laevis Eggs Laboratory manuals Xenopus embryology Laboratory Manuals Krallenfrosch (DE-588)4165479-1 gnd Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4165479-1 (DE-588)4039983-7 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Xenopus protocols cell biology and signal transduction |
| title_auth | Xenopus protocols cell biology and signal transduction |
| title_exact_search | Xenopus protocols cell biology and signal transduction |
| title_exact_search_txtP | Xenopus protocols cell biology and signal transduction |
| title_full | Xenopus protocols cell biology and signal transduction ed. by X. Johné Liu |
| title_fullStr | Xenopus protocols cell biology and signal transduction ed. by X. Johné Liu |
| title_full_unstemmed | Xenopus protocols cell biology and signal transduction ed. by X. Johné Liu |
| title_short | Xenopus protocols |
| title_sort | xenopus protocols cell biology and signal transduction |
| title_sub | cell biology and signal transduction |
| topic | Xenopus cabt cellular biology cabt oocytes cabt genetic analysis cabt laboratory methods cabt Cellular signal transduction Laboratory manuals Oocytes cytology Laboratory Manuals Signal Transduction Laboratory Manuals Xenopus laevis Cytology Laboratory manuals Xenopus laevis Eggs Laboratory manuals Xenopus embryology Laboratory Manuals Krallenfrosch (DE-588)4165479-1 gnd Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Xenopus cellular biology oocytes genetic analysis laboratory methods Cellular signal transduction Laboratory manuals Oocytes cytology Laboratory Manuals Signal Transduction Laboratory Manuals Xenopus laevis Cytology Laboratory manuals Xenopus laevis Eggs Laboratory manuals Xenopus embryology Laboratory Manuals Krallenfrosch Molekularbiologie Methode Aufsatzsammlung |
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