PRINS and In Situ PCR Protocols:
Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investiga...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
334 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology |
| Beschreibung: | 1 Online-Ressource (XVI, 244 p) |
| ISBN: | 9781597450683 |
| DOI: | 10.1385/1597450685 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044950817 | ||
| 003 | DE-604 | ||
| 005 | 20181002 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2006 |||| o||u| ||||||eng d | ||
| 020 | |a 9781597450683 |9 978-1-59745-068-3 | ||
| 024 | 7 | |a 10.1385/1597450685 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59745-068-3 | ||
| 035 | |a (OCoLC)730584261 | ||
| 035 | |a (DE-599)BVBBV044950817 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 050 | 0 | |a QH452.8 | |
| 082 | 0 | |a 572.8/4 |2 22 | |
| 082 | 0 | |a 572 |2 23 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a BIO 180f |2 stub | ||
| 245 | 1 | 0 | |a PRINS and In Situ PCR Protocols |c edited by Franck Pellestor |
| 250 | |a Second Edition | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a 1 Online-Ressource (XVI, 244 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 334 | |
| 520 | |a Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Biochemistry, general | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Biochemistry | |
| 650 | 4 | |a Cell biology | |
| 650 | 0 | 7 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Primer |g Molekulargenetik |0 (DE-588)4318719-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Primer |g Molekulargenetik |0 (DE-588)4318719-5 |D s |
| 689 | 0 | 1 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |D s |
| 689 | 0 | 2 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Pellestor, Franck |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781588295491 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/1597450685 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343576 | ||
| 966 | e | |u https://doi.org/10.1385/1597450685 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1385/1597450685 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178538695229440 |
|---|---|
| any_adam_object | |
| author2 | Pellestor, Franck |
| author2_role | edt |
| author2_variant | f p fp |
| author_facet | Pellestor, Franck |
| building | Verbundindex |
| bvnumber | BV044950817 |
| callnumber-first | Q - Science |
| callnumber-label | QH452 |
| callnumber-raw | QH452.8 |
| callnumber-search | QH452.8 |
| callnumber-sort | QH 3452.8 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 4150 |
| classification_tum | BIO 180f |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59745-068-3 (OCoLC)730584261 (DE-599)BVBBV044950817 |
| dewey-full | 572.8/4 572 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8/4 572 |
| dewey-search | 572.8/4 572 |
| dewey-sort | 3572.8 14 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/1597450685 |
| edition | Second Edition |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03633nmm a2200589zcb4500</leader><controlfield tag="001">BV044950817</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20181002 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2006 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781597450683</subfield><subfield code="9">978-1-59745-068-3</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/1597450685</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59745-068-3</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)730584261</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044950817</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH452.8</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.8/4</subfield><subfield code="2">22</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 180f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">PRINS and In Situ PCR Protocols</subfield><subfield code="c">edited by Franck Pellestor</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">Second Edition</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2006</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XVI, 244 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">334</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Primer</subfield><subfield code="g">Molekulargenetik</subfield><subfield code="0">(DE-588)4318719-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Primer</subfield><subfield code="g">Molekulargenetik</subfield><subfield code="0">(DE-588)4318719-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pellestor, Franck</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781588295491</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/1597450685</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343576</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1597450685</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1597450685</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044950817 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781597450683 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343576 |
| oclc_num | 730584261 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XVI, 244 p) |
| psigel | ZDB-2-PRO |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | PRINS and In Situ PCR Protocols edited by Franck Pellestor Second Edition Totowa, NJ Humana Press 2006 1 Online-Ressource (XVI, 244 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 334 Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Primer Molekulargenetik (DE-588)4318719-5 gnd rswk-swf Primer Molekulargenetik (DE-588)4318719-5 s Polymerase-Kettenreaktion (DE-588)4256726-9 s Methode (DE-588)4038971-6 s b DE-604 Pellestor, Franck edt Erscheint auch als Druck-Ausgabe 9781588295491 https://doi.org/10.1385/1597450685 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | PRINS and In Situ PCR Protocols Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd Primer Molekulargenetik (DE-588)4318719-5 gnd |
| subject_GND | (DE-588)4256726-9 (DE-588)4038971-6 (DE-588)4318719-5 |
| title | PRINS and In Situ PCR Protocols |
| title_auth | PRINS and In Situ PCR Protocols |
| title_exact_search | PRINS and In Situ PCR Protocols |
| title_full | PRINS and In Situ PCR Protocols edited by Franck Pellestor |
| title_fullStr | PRINS and In Situ PCR Protocols edited by Franck Pellestor |
| title_full_unstemmed | PRINS and In Situ PCR Protocols edited by Franck Pellestor |
| title_short | PRINS and In Situ PCR Protocols |
| title_sort | prins and in situ pcr protocols |
| topic | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Methode (DE-588)4038971-6 gnd Primer Molekulargenetik (DE-588)4318719-5 gnd |
| topic_facet | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Polymerase-Kettenreaktion Methode Primer Molekulargenetik |
| url | https://doi.org/10.1385/1597450685 |
| work_keys_str_mv | AT pellestorfranck prinsandinsitupcrprotocols |