PCR Protocols:
Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine toda...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
226 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications |
| Beschreibung: | 1 Online-Ressource (556 p) |
| ISBN: | 9781592593842 |
| DOI: | 10.1385/1592593844 |
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Datensatz im Suchindex
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| edition | Second Edition |
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| spelling | PCR Protocols edited by John M. S. Bartlett, David Stirling Second Edition Totowa, NJ Humana Press 2003 1 Online-Ressource (556 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 226 Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications Life Sciences Cell Biology Life sciences Cell biology Methode (DE-588)4038971-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Methode (DE-588)4038971-6 s Bartlett, John M. S. edt Stirling, David edt Erscheint auch als Druck-Ausgabe 9780896036420 https://doi.org/10.1385/1592593844 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | PCR Protocols Life Sciences Cell Biology Life sciences Cell biology Methode (DE-588)4038971-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4256726-9 (DE-588)4143413-4 |
| title | PCR Protocols |
| title_auth | PCR Protocols |
| title_exact_search | PCR Protocols |
| title_full | PCR Protocols edited by John M. S. Bartlett, David Stirling |
| title_fullStr | PCR Protocols edited by John M. S. Bartlett, David Stirling |
| title_full_unstemmed | PCR Protocols edited by John M. S. Bartlett, David Stirling |
| title_short | PCR Protocols |
| title_sort | pcr protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Methode (DE-588)4038971-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Methode Polymerase-Kettenreaktion Aufsatzsammlung |
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