Since the cloning of the cystic fibrosis transmembrane conductance regulator (CFTR) nearly a decade ago, cystic fibrosis researchers, clinicians, and patients have come to rely increasingly on a diverse array of fundamental techniques to understand the molecular basis of this complex disease. Cystic...

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Bibliographic Details

Other Authors:	Skach, William R. (Editor)
Format:	Electronic eBook
Language:	English
Published:	Totowa, NJ Humana Press 2002
Series:	Methods in Molecular Medicine™ 70
Subjects:	Medicine & Public Health Pneumology/Respiratory System Medicine Respiratory organs / Diseases Mukoviszidose Molekulare Medizin
Online Access:	UBR01 TUM01 Volltext
Summary:	Since the cloning of the cystic fibrosis transmembrane conductance regulator (CFTR) nearly a decade ago, cystic fibrosis researchers, clinicians, and patients have come to rely increasingly on a diverse array of fundamental techniques to understand the molecular basis of this complex disease. Cystic Fibrosis Methods and Protocols consolidates a broad range of detailed and readily reproducible in vitro, cellular, and whole animal laboratory protocols into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo protocols to examine how CFTR dysfunction produces multisystem pathology in human and animal models. Comprehensive, multidisciplinary, and highly practical, Cystic Fibrosis Methods and Protocols makes accessible to today's cystic fibrosis investigator the powerful new scientific techniques required to investigate the basic science of the disease and to translate this into effective clinical solutions
Physical Description:	1 Online-Ressource (XVI, 615 p)
ISBN:	9781592591879
DOI:	10.1385/1592591876