Verfügbarkeit: Mitotic spindle

Mitotic spindle: methods and protocols

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Bibliographische Detailangaben
Weitere Verfasser:	Chang, Paul (HerausgeberIn), Ohi, Ryoma (HerausgeberIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	New York Humana Press [2016]
Schriftenreihe:	Methods in molecular biology 1413
Schlagworte:	Molekularbiologische Methode Mitose Kernspindel
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	xiii, 424 Seiten Illustrationen, Diagramme
ISBN:	9781493935406

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Datensatz im Suchindex

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adam_text	Titel: The mitotic spindle Autor: Chang, Paul Jahr: 2016 Contents Preface.......................................................... p Contributors.......................................................... xi Part I Methods Focused on Cytology 1 Using Fluorescence Microscopy to Study Mitosis...................... 3 Sat K. Balchand, Barbara J. Mann, and Patricia Wadsworth 2 Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation.................................... 15 Bin He and Daniela Cimini Part II Methods Focused on Microtubules and the Mitotic Spindle 3 Purification and Fluorescent Labeling of Tubulin from Xenopus laevis Egg Extracts.................................................. 35 Aaron C. Groen and Timothy J. Mitchison 4 Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro................................. 47 Marija Zanic 5 Imaging and Quantifying the Dynamics of y-Tubulin at Microtubule Minus Ends in Mitotic Spindles.................................... 63 Nicolas Lecland and Jens LUders 6 Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy.............. 77 Matthew King and Sabine Petry 7 Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions in Synthetic Cell-Like Compartments with Tunable Size......... 87 Matthew C. Good Part III Methods Focused on Kjnetochores AND THE KlNETOCHORE-MlCROTUBULE INTERFACE 8 In Vitro Kinetochore Assembly.................................... Ill Matthew D.D. Miell and Aaron F. Straight 9 Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes........................................ 135 Tatsuya Nishino and Tatsuo Fukagawa 10 Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells............................................... 147 Keith F. DeLuca, Jacob A. Herman, and Jennifer G. DeLuca vii viii Contents 11 Studying Kinetochores In Vivo Using FLIM-FRET..................... 169 Toe Yeon Yoo and Daniel J. Needleman Part IV Methods Focused on the Spindle Pole 12 Purification of Fiuorescently Labeled Saccharomyces cerevisiae Spindle Pole Bodies............................................. 189 Kimberly K. Fang, Beth Graczyk, and Trisha N. Davis 13 A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole-to-Centrosome Conversion............................ 197 Rajesh Kumar Soni and Meng-Fu Bryan Tsou 14 Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation................................................ 207 Vladimir Joukov, Johannes C. Walter, and Arcangela De Nicolo Part V Methods Focused on the Cellular Functions of Microtubule Motor Proteins 15 Analyzing Spindle Positioning Dynamics in Cultured Cells............... 239 Tomomi Kiyomitsu 16 Quantification of Mitotic Chromosome Alignment..................... 253 Cindy Fonseca and Jason Stumpff 17 Imaging Mitosis in the Moss Physcomitrella patens...................... 263 Mod Yamada, Tomohiro Miki, and Gohta Goshima 18 Small Molecule Approach to Study the Function of Mitotic Kinesins........ 283 Naowras Al-Obaidi, Johanna Kastl, and Thomas U. Mayer Part VI Novel Approaches to Study Spindle Function and Regulation 19 Identification and Characterization of Mitotic Spindle-Localized Transcripts................................................... 303 Amy B. Emerman, Ashwini Jambhekar, and Michael D. Blower 20 Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization..................... 325 Edward R. Ballister and Michael A. Lampson 21 Studying Kinetochore Kinases..................................... 333 Adrian T. Saurin and Geert J.P.L. Kops 22 Engineering and Functional Analysis of Mitotic Kinases Through Chemical Genetics...................................... 349 Mathew J.K. Jones and Prasad V. Jallepalli Part VII The Mitotic Spindle and Cancer 23 Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer........................... 367 Mijung Kwon Contents ix 24 Generation and Purification of Tetraploid Cells................................................393 Elizabeth M. Shenk and Neil J. Ganem 25 Anti-Microtubule Drugs..................................................................................403 Stefan Florian and Timothy J. Mitchison Index..............................................................................................................................423
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spelling	Mitotic spindle methods and protocols edited by Paul Chang, Massachusetts Institute of Technology, Cambridge, MA, USA; Ryoma Ohi, Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA New York Humana Press [2016] 2016 xiii, 424 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 1413 Springer protocols Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Mitose (DE-588)4170185-9 gnd rswk-swf Kernspindel (DE-588)4206805-8 gnd rswk-swf Mitose (DE-588)4170185-9 s Kernspindel (DE-588)4206805-8 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Chang, Paul edt Ohi, Ryoma edt Erscheint auch als Online-Ausgabe 978-1-4939-3542-0 Methods in molecular biology 1413 (DE-604)BV035362695 1413 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=028841246&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Mitotic spindle methods and protocols Methods in molecular biology Molekularbiologische Methode (DE-588)7650063-9 gnd Mitose (DE-588)4170185-9 gnd Kernspindel (DE-588)4206805-8 gnd
subject_GND	(DE-588)7650063-9 (DE-588)4170185-9 (DE-588)4206805-8
title	Mitotic spindle methods and protocols
title_auth	Mitotic spindle methods and protocols
title_exact_search	Mitotic spindle methods and protocols
title_full	Mitotic spindle methods and protocols edited by Paul Chang, Massachusetts Institute of Technology, Cambridge, MA, USA; Ryoma Ohi, Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA
title_fullStr	Mitotic spindle methods and protocols edited by Paul Chang, Massachusetts Institute of Technology, Cambridge, MA, USA; Ryoma Ohi, Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA
title_full_unstemmed	Mitotic spindle methods and protocols edited by Paul Chang, Massachusetts Institute of Technology, Cambridge, MA, USA; Ryoma Ohi, Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA
title_short	Mitotic spindle
title_sort	mitotic spindle methods and protocols
title_sub	methods and protocols
topic	Molekularbiologische Methode (DE-588)7650063-9 gnd Mitose (DE-588)4170185-9 gnd Kernspindel (DE-588)4206805-8 gnd
topic_facet	Molekularbiologische Methode Mitose Kernspindel
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work_keys_str_mv	AT changpaul mitoticspindlemethodsandprotocols AT ohiryoma mitoticspindlemethodsandprotocols

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