Zelluläre Kardiomyoplastie im Mausmodell:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
2004
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 107 S. Ill., graph. Darst. 21 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | Table of Content
1. Introduction 1
1.1. Cellular replacement therapy I
1.2 Application of cell types in cellular replacement therapy 2
1.2.1 Ventricular cardiomyocytes and Establishment of transgenic mouse model 2
1.2.1.1 Neonatal, fetal and adult cardiomyocytes 2
1.2.1.2 Establishment of transgenic mouse model 3
1.2.2 Stem cell and its potential therapy 5
1.2.2.1 Mmurine embryonic stem cells 5
1.2.2.2 Stem cell - bone marrow stem cells 7
1.2.2.3 Therapeutic potential of stem cells 8
1.2.3 Autologous skeletal myoblast 8
1.2.4 Transfected ES cells and differentiation into desired direction in vitro and
Vivo 10
1.2.4.1 Establishment of transfected ES cell system 10
1.2.4.2 a-MHC ES cell-derived cardiomyocytes 10
1.2.4.3 (i-actin ES cell-derived neuron precursor cells 12
1.2.4.4 Development of ES cells and predifferentiation cells in syngenetic vivo 14
1.3 Establishment of the novel surgical technique of cellular cardiomyoplasty in
Mouse model 14
2. Aims and questions addressed in the study 16
3. Materials and methods 18
3.1 Cells 18
3.1.1 Murine embryonic ventricular cardiomyocytes 18
3.1.1.1 Mouse breeding 18
3.1.1.2 Harvesting of embryonic cardiomyocytes and Flow Cytometry 18
3.1.2 Murine embryonic stem cells in vitro 20
3.1.2.1 Cell culture of undifferentiated ES cells 20
3.1.2.2 Predifferentiation in vitro 21
3.1.2.2.1 ES cell -derived cardiomyocyte 21
3.1.2.2.2 Harvesting the beating clusters out of the outgrowth EBs 23
3.1.2.2.3 ES cell-derived neural precursor cells 23
3.1.2.2.4 Monitoring the capacity of selected neuronal precursor cells on vitro 25
3.2 Cellular transplantation 25
3.2.1 Heart transplantation in mice 25
3.2.2 Brain operation in mice 28
3.3 Immunocytochemistry 29
3.3.1 Oct4 staining of undifferentiated ES cells and predifferentiated ES cells in
Vivo 29
3.3.2 Nestin, NeuN staining of ES cell- derived neural cells 29
3.4 Histology, immunohistochemistry, and Western Blotting 30
3.5 Electronmicroscopy 31
3.6 Heart functional measurement in embryonic cardiomyocytes
transplantation 31
3.6.1 Electrophysiology 31
3.6.2 Isometric tension measurements 32
3.6.3 Echocardiography 33
4. Statistics 34
5. Results 35
5.1 Embryonic cardiomyocyte cardiomyoplasty 35
5.1.1 Signal cardiomyocytes from transgenic embryos and FACS measurement 35
5.1.2 Detection of cryinfarcted lesions and pathology 37
5.1.3 Identification of transplanted cells, engraftment, and differentiation 40
5.1.4 Intercalated disks formation of transplanted cardiomyocytes
with host tissue 42
5.1.5 Detection of the Tropnin I expression in embryonic cardiomyocyte- treated
Heart tissue 44
5.1.6 Neovascularization in cryoinfarcted myocardium 45
5.1.7 Functional characteristics of embryonic cardiomyocyte cardiomyoplasty 46
5.1.7.1 Electrophysiological study 46
5.1.7.2 Contractility and Heart Function 50
5.1.8 Animal survival 55
5.2 Embryonic stem cell cardiomyoplasty 56
5.2.1 Oct4 expression of undifferentiatedES cell clones in vitro 56
5.2.2 Predifferentiation of beating clusters from ES cells in vitro 57
5.2.3 Tumorigenicity of ES cell in cardiomyoplasty 59
5.2.4 ES cell-derived neural progenitors and their transplantation into brains
following occlusion of MCA 63
5.2.5 Therapeutic potential of puromycin-selected cardiomyocytes 67
6. Discussion 73
6.1 The therapeutic potential of embryonic ventricular cardiomyocytes in mouse
model 73
6.2 The therapeutic potential research of murine undifferentiated ES cell and
predifferentiated ES cell in mouse model 78
7. Summary 82
8. Zussammenfassung 84
9. References 86
10 Publications 104
11 Curriculum Vitae 106
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| indexdate | 2024-07-09T20:02:05Z |
| institution | BVB |
| language | German |
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| physical | 107 S. Ill., graph. Darst. 21 cm |
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| spelling | Xia, Ying 1963- Verfasser (DE-588)129291048 aut Zelluläre Kardiomyoplastie im Mausmodell vorgelegt von Ying Xia 2004 107 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 2004 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012976052&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Xia, Ying 1963- Zelluläre Kardiomyoplastie im Mausmodell |
| subject_GND | (DE-588)4113937-9 |
| title | Zelluläre Kardiomyoplastie im Mausmodell |
| title_auth | Zelluläre Kardiomyoplastie im Mausmodell |
| title_exact_search | Zelluläre Kardiomyoplastie im Mausmodell |
| title_full | Zelluläre Kardiomyoplastie im Mausmodell vorgelegt von Ying Xia |
| title_fullStr | Zelluläre Kardiomyoplastie im Mausmodell vorgelegt von Ying Xia |
| title_full_unstemmed | Zelluläre Kardiomyoplastie im Mausmodell vorgelegt von Ying Xia |
| title_short | Zelluläre Kardiomyoplastie im Mausmodell |
| title_sort | zellulare kardiomyoplastie im mausmodell |
| topic_facet | Hochschulschrift |
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