Ribozymes and siRNA protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
252 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 623 S. Ill., graph. Darst. |
| ISBN: | 1588292266 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804132802647556096 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
1 Ribozyme and siRNA Mediated mRNA Degradation:
A General Introduction
Mouldy Sioud /
2 Combination of Chemical and Enzymatic RNA Synthesis
Rajesh K. Gaur, Andreas Hanne, and Guido Krupp 9
3 Determination of Kinetic Parameters for Hammerhead
and Hairpin Ribozymes
Martha J. Fedor 19
4 Real Time Monitoring of RNA and DNA Reactions
by Fluorescence Detection
Kumud K. Singh, Andreas Hanne, and Guido Krupp 33
5 Quantification of Ribozyme Target RNA Using Real Time PCR
Dagmar Klein, CamiHo Ricordi, and Ricardo L. Pastori 49
6 Analysis of Ribozyme Structure and Function
by Nucleotide Analog Interference Mapping
Soumitra Basu, Catherine Pazsint, and Gopeswar Chowdhury 57
7 Analysis of Global Conformational Transitions in Ribozymes
David M. J. Lilley 77
8 In Vivo Detection of Ribozyme Cleavage Products
and RNA Structure by Use of Terminal Transferase Dependent PCR
Hsiu Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi,
and Arthur D. Riggs 109
9 Identification of Efficient Cleavage Sites in Long Target RNAs
Wei Hua Pan and Gary A. Clawson 125
10 Selection In Vitro of Allosteric Ribozymes
Adam Roth and Ronald R. Breaker 145
11 Regulation of Ribozyme Cleavage Activity by Oligonucleotides
Yasuo Komatsu and Eiko Ohtsuka 165
12 Tetracycline Regulated Expression of Hammerhead Ribozymes In Vivo
Emma T. Bowden and Anna T. Riegel 179
vii
viii Contents
13 Ribozyme Expression Systems
Masayuki Sano and Kazunari Taira 195
14 Design and Expression of Chimeric U1/Ribozyme Transgenes
Roger Abounader, Robert Montgomery, Harry Dietz,
and John Laterra 209
15 Design and Validation of Therapeutic Hammerhead Ribozymes
for Autosomal Dominant Diseases
Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin,
and William W. Hauswirth 221
16 Helicase Attached Novel Hybrid Ribozymes
Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara,
and Kazunari Taira 237
17 Functional Gene Discovery Using Hybrid Ribozyme Libraries
Yosbio Kato, Masaru Tsunemi, Makoto Miyagishi,
Hiroaki Kawasaki, and Kazunari Taira 245
18 Maxizyme Technology
Mayu lyo, Hiroaki Kawasaki, and Kazunari Taira 257
19 Target Site Selection for the 10 23 DNAzyme
Murray J. Cairns and Lun Quan Sun 267
20 In Vitro Selected RNA Cleaving DNA Enzymes
From Combinatorial Libraries
Samitabh Cbakraborti, Bandi Sriram and Akhil C. Banerjea 279
21 Nucleic Acid Sequence Analysis Using DNAzymes
Murray f. Cairns and Lun Quan Sun 291
22 Crystallization of the Hairpin Ribozyme: Illustrative Protocols
Peter B. Rupert and Adrian R. Ferre D Amare 303
23 An Experimental Method for Selecting Effective Target Sites
and Designing Hairpin Ribozymes
Alicia Barroso deljesus, Flena Puerta Fernandez,
Cristina Romero Lopez, and Alfredo Berzal Herranz 313
24 Design and Optimization of Sequence Specific Hairpin Ribozymes
Cristina Romero Lopez, Alicia Barroso deljesus,
Elena Puerta Fernandez, and Alfredo Berzal Herranz 327
25 Design, Targeting, and Initial Screening of sTRSV Derived Hairpin
Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency
In Vitro
Max W. Richardson, Linda Hostalek, Michelle Dobson, Jason Hu,
Richard Shippy, Andrew Siwkowski, Jonathan D. Marmur,
Kamel Khalili, Paul E. Klotman, Arnold Hampel,
and Jay Rappaport 339
Contents ix
26 Optimization and Application of the Group I Ribozyme
Trans Splicing Reaction
Christer Einvik, Tonje Fiskaa, Eirik W. Lundblad,
and Steinar johansen 359
27 Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts
by Trans Splicing Ribozymes
Leonidas A. Phylactou 373
28 General Design and Construction of RNase P Ribozymes
for Gene Targeting Application
Hua Zou, Karen Chan, Phong Trang, and Fenyong Liu 385
29 In Vitro Selection of RNase P Ribozymes That Efficiently Cleave
a Target mRNA
Kihoon Kim and Fenyong Liu 399
30 In Vitro Selection of External Guide Sequences
for Directing Human RNase P to Cleave a Target mRNA
Stephen Raj and Fenyong Liu 413
31 RNase P Mediated Inhibition of Viral Growth
by Exogenous Administration of Short Oligonucleotide
External Guide Sequence
Walter Dunn and Fenyong Liu 425
32 RNase P Ribozyme As an Antiviral Agent Against
Human Cytomegalovirus
Phong Trang and Fenyong Liu 437
33 Inhibition of Gene Expression by Nucleic Acid Enzymes
in Rodent Models of Human Disease
Per Ole Iversen and Mouldy Sioud 451
34 Potential Design Rules and Enzymatic Synthesis of siRNAs
Mouldy Sioud and Marianne Leirdal 457
35 RNA Interference (RNAi) With RNase Ill Prepared siRNAs
Dun Yang, Andrei Goga, and J. Michael Bishop 471
36 RNAi Expression Vectors in Mammalian Cells
Makoto Miyagishi and Kazunari Taira 483
37 Gene Array Analysis of Glioma Cells After Treatment
With Anti PKC asiRNA
Marianne Leirdal and Mouldy Sioud 493
38 RNAi in Living Mice
Hidetoshi Hasuwa and Masaru Okabe 507
39 Construction and Transfection of PCR Products Expressing siRNAs
or shRNAs in Mammalian Cells
Daniela Castanotto and John J. Rossi 509
x Contents
40 Systemic Delivery of siRNAs
Mouldy Sioud and Dag R. S0rensen 515
41 Adenovirus Delivered siRNA
Changxian Shen and Sven N. Reske 523
42 RNAi Expression Vectors in Plant Cells
Hideo Akashi, Makoto Miyagishi, and Kazunari Taira 533
43 Delivery Agents for Oligonucleotides
Olivier Seksek and Jacques Bolard 545
44 Selection of Peptides for Specific Delivery of Oligonucleotides
Into Cancer Cells
Mohsen Shadidi and Mouldy Sioud 569
45 Clinical Gene Therapy Research Utilizing Ribozymes:
Application to the Treatment of HIV/AIDS
Frances K. Ngok, Ronald T. Mitsuyasu, Janet L. Macpherson,
Maureen P. Boyd, Geoff P. Symonds, and Rafael G. Amado 581
46 Critical Steps in the Implementation of Hematopoietic
Progenitor Cell Gene Therapy Using Ribozyme Vectors:
A Laboratory Protocol
Maureen P. Boyd, Frances K. Ngok, Alison V. Todd,
Geoff P. Symonds, Janet L. Macpherson, and Rafael G. Amado 599
Index 617
|
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| spelling | Ribozymes and siRNA protocols Ed. by Mouldy Sioud 2. ed. Totowa, NJ Humana Press 2004 XV, 623 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 252 Petits ARN interférents - Manuels de laboratoire Ribozymes - Manuels de laboratoire Ribozymes gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Catalytic RNA Laboratory manuals Gene Expression Profiling Laboratory Manuals Gene Therapy Laboratory Manuals RNA, Catalytic Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Ribozym (DE-588)4356469-0 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ribozym (DE-588)4356469-0 s Labortechnik (DE-588)4123602-6 s b DE-604 Sioud, Mouldy Sonstige oth Methods in molecular biology 252 (DE-604)BV035362695 252 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=012829665&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ribozymes and siRNA protocols Methods in molecular biology Petits ARN interférents - Manuels de laboratoire Ribozymes - Manuels de laboratoire Ribozymes gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Catalytic RNA Laboratory manuals Gene Expression Profiling Laboratory Manuals Gene Therapy Laboratory Manuals RNA, Catalytic Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Ribozym (DE-588)4356469-0 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4356469-0 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Ribozymes and siRNA protocols |
| title_auth | Ribozymes and siRNA protocols |
| title_exact_search | Ribozymes and siRNA protocols |
| title_full | Ribozymes and siRNA protocols Ed. by Mouldy Sioud |
| title_fullStr | Ribozymes and siRNA protocols Ed. by Mouldy Sioud |
| title_full_unstemmed | Ribozymes and siRNA protocols Ed. by Mouldy Sioud |
| title_short | Ribozymes and siRNA protocols |
| title_sort | ribozymes and sirna protocols |
| topic | Petits ARN interférents - Manuels de laboratoire Ribozymes - Manuels de laboratoire Ribozymes gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Catalytic RNA Laboratory manuals Gene Expression Profiling Laboratory Manuals Gene Therapy Laboratory Manuals RNA, Catalytic Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Ribozym (DE-588)4356469-0 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Petits ARN interférents - Manuels de laboratoire Ribozymes - Manuels de laboratoire Ribozymes Wetenschappelijke technieken Wissenschaftliches Arbeiten Catalytic RNA Laboratory manuals Gene Expression Profiling Laboratory Manuals Gene Therapy Laboratory Manuals RNA, Catalytic Laboratory Manuals RNA, Small Interfering Laboratory Manuals Small interfering RNA Laboratory manuals Ribozym Labortechnik Aufsatzsammlung |
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