Riboswitches: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York
Humana
2009
|
| Schriftenreihe: | Methods in molecular biology
540 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index Predicting riboswitch regulation on a genomic scale / J.E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications / R. Rieder, C. Hobartner and R. Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / B. Heppell ... [et al.] -- Transcriptional approaches to riboswitch studies / A. Mironov, V. Epshtein and E. Nudler -- Kinetics of riboswitch regulation studied by in vitro transcription / J.K. Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / J.F. Lemay ... [et al.] -- Methods for analysis of ligand-induced RNA conformational changes / C.A. Brautigam, C.A. Wakeman and W.C. Winkler -- Monitoring RNA-ligand interactions using isothermal titration calorimetry / S.D. Gilbert and R.T. Batey -- Preparation and crystallization of riboswitch-ligand complexes / O. Pikovskaya ... [et al.] -- Crystallization of the glmS ribozyme-riboswitch / D.J. Klein and A.R. Ferre-D'Amare -- Riboswitch conformations revealed by small-angle X-ray scattering / J. Lipfert, D. Herschlag and S. Doniach -- Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches / J. Buck ... [et al.] -- Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / C.A. Wakeman and W.C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / J.K. Soukup and G.A. Soukup -- RNA-dependent RNA switches in bacteria / T.M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / C. Chevalier ... [et al.] -- Structural probing of RNA thermosensors / C. Chiaruttini, F. Allem and M. Springer -- Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / P. Fechter ... [et al.] -- Isolation and characterization of the heat shock RNA 1 / I. Shamovsky and E. Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / T.M. Henkin -- In vitro selection of conformational probes for riboswitches / G. Mayer and M. Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / J.H. Urban and J. Vogel -- High-throughput screens to discover synthetic riboswitches / S.A. Lynch, S. Topp and J.P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / L. Yen, B.R. Stockwell and R.C. Mulligan -- In vitro selection of glmS ribozymes / K.H. Link and R.R. Breaker |
| Beschreibung: | XI, 366 S. Ill., graph. Darst. 27 cm |
Internformat
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| 245 | 1 | 0 | |a Riboswitches |b methods and protocols |c ed. by Alexander Serganov |
| 264 | 1 | |a New York |b Humana |c 2009 | |
| 300 | |a XI, 366 S. |b Ill., graph. Darst. |c 27 cm | ||
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| 490 | 1 | |a Methods in molecular biology |v 540 | |
| 500 | |a Includes bibliographical references and index | ||
| 500 | |a Predicting riboswitch regulation on a genomic scale / J.E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications / R. Rieder, C. Hobartner and R. Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / B. Heppell ... [et al.] -- Transcriptional approaches to riboswitch studies / A. Mironov, V. Epshtein and E. Nudler -- Kinetics of riboswitch regulation studied by in vitro transcription / J.K. Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / J.F. Lemay ... [et al.] -- Methods for analysis of ligand-induced RNA conformational changes / C.A. Brautigam, C.A. Wakeman and W.C. Winkler -- Monitoring RNA-ligand interactions using isothermal titration calorimetry / S.D. Gilbert and R.T. Batey -- Preparation and crystallization of riboswitch-ligand complexes / O. Pikovskaya ... [et al.] -- | ||
| 500 | |a Crystallization of the glmS ribozyme-riboswitch / D.J. Klein and A.R. Ferre-D'Amare -- Riboswitch conformations revealed by small-angle X-ray scattering / J. Lipfert, D. Herschlag and S. Doniach -- Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches / J. Buck ... [et al.] -- Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / C.A. Wakeman and W.C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / J.K. Soukup and G.A. Soukup -- RNA-dependent RNA switches in bacteria / T.M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / C. Chevalier ... [et al.] -- Structural probing of RNA thermosensors / C. Chiaruttini, F. Allem and M. Springer -- | ||
| 500 | |a Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / P. Fechter ... [et al.] -- Isolation and characterization of the heat shock RNA 1 / I. Shamovsky and E. Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / T.M. Henkin -- In vitro selection of conformational probes for riboswitches / G. Mayer and M. Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / J.H. Urban and J. Vogel -- High-throughput screens to discover synthetic riboswitches / S.A. Lynch, S. Topp and J.P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / L. Yen, B.R. Stockwell and R.C. Mulligan -- In vitro selection of glmS ribozymes / K.H. Link and R.R. Breaker | ||
| 650 | 4 | |a RNA. | |
| 650 | 4 | |a RNA-protein interactions | |
| 650 | 4 | |a Messenger RNA. | |
| 650 | 4 | |a Ligands (Biochemistry) | |
| 650 | 4 | |a RNA / chemistry / Laboratory Manuals | |
| 650 | 4 | |a Gene Expression Regulation / physiology / Laboratory Manuals | |
| 650 | 4 | |a Laboratory Techniques and Procedures / Laboratory Manuals | |
| 650 | 4 | |a Gene Expression Regulation |x physiology |v Laboratory Manuals | |
| 650 | 4 | |a Laboratory Techniques and Procedures |v Laboratory Manuals | |
| 650 | 4 | |a Ligands (Biochemistry) | |
| 650 | 4 | |a Messenger RNA | |
| 650 | 4 | |a RNA | |
| 650 | 4 | |a RNA |x chemistry |v Laboratory Manuals | |
| 650 | 4 | |a RNA-protein interactions | |
| 700 | 1 | |a Serganov, Alexander |4 edt | |
| 830 | 0 | |a Methods in molecular biology |v 540 |w (DE-604)BV035362695 |9 540 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-017622084 | ||
Datensatz im Suchindex
| _version_ | 1804139217856495616 |
|---|---|
| adam_text | METHODS IN MOLECULAR BIOLOGY* RIBOSWITCHES METHODS AND PROTOCOLS EDITED
BY ALEXANDER SERGANOV DEPARTMENT OF STRUCTURAL BIOLOGY, MEMORIAL
SLOAN-KETTERING CANCER CENTER NEW YORK, NY, USA $), C HUMANA PRESS
CONTENTS PREFACE V CONTRIBUTORS IX 1 PREDICTING RIBOSWITCH REGULATION ON
A GENOMIC SCALE 1 JEFFREY E. BARRICK 2 ENZYMATIC LIGATION STRATEGIES FOR
THE PREPARATION OF PURINE RIBOSWITCHES WITH SITE-SPECIFIC CHEMICAL
MODIFICATIONS 15 RENATE RIEDER, CLAUDIA HBBARTNER, AND RONALD MICURA 3
APPLICATION OF FLUORESCENT MEASUREMENTS FOR CHARACTERIZATION OF
RIBOSWITCH-LIGAND INTERACTIONS 25 BENOIT HEPPELL, JEROME MULBBACHER, J.
CARLOS PENEDO, AND DANIEL A. LAFONTAINE 4 TRANSCRIPTIONAL APPROACHES TO
RIBOSWITCH STUDIES 39 ALEXANDER MIRONOV, VITALY EPSHTEIN, AND EVGENY
NUDLER 5 KINETICS OF RIBOSWITCH REGULATION STUDIED BY IN VITRO
TRANSCRIPTION 53 /. KENNETH WICKISER 6 MOLECULAR BASIS OF RNA-MEDIATED
GENE REGULATION ON THE ADENINE RIBOSWITCH BY SINGLE-MOLECULE APPROACHES
65 JEAN-FRANCOIS LEMAY, J. CARLOS PENEDO, JEROME MULHBACHER, AND DANIEL
A. LAFONTAINE 7 METHODS FOR ANALYSIS OF LIGAND-INDUCED RNA
CONFORMATIONAL CHANGES 77 CHAD A. BRAUTIGAM, CATHERINE A. WAKEMAN, AND
WADE C. WINKLER 8 MONITORING RNA-LIGAND INTERACTIONS USING ISOTHERMAL
TITRATION CALORIMETRY 97 SUNNY D. GILBERT AND ROBERT T. BATEY 9
PREPARATION AND CRYSTALLIZATION OF RIBOSWITCH-LIGAND COMPLEXES 115 OLGA
PIKOVSKAYA, ARTEM A. SERGANOV, ANN POLONSKAIA, ALEXANDER SERGANOV, AND
DINSHAW J. PATEL 10 CRYSTALLIZATION OF THE^/WSRIBOZYME-RIBOSWITCH 129
DANIEL J. KLEIN AND ADRIAN R. FERRE-D AMARE 11 RIBOSWITCH CONFORMATIONS
REVEALED BY SMALL-ANGLE X-RAY SCATTERING 141 JAN LIPFERT, DANIEL
HERSCHLAG, AND SEBASTIAN DONIACH 12 TIME-RESOLVED NMR SPECTROSCOPY:
LIGAND-INDUCED REFOLDING OF RIBOSWITCHES 161 JANINA BUCK, BORIS FIIRTIG,
JONAS NOESKE, JENS WOHNERT, AND HARALD SCHWALBE 13 ANALYSIS OF THE RNA
BACKBONE: STRUCTURAL ANALYSIS OF RIBOSWITCHES BY IN-LINE PROBING AND
SELECTIVE 2 -HYDROXYL ACYLATION AND PRIMER EXTENSION (SHAPE) 173
CATHERINE A. WAKEMAN AND WADE C. WINKLER VII VIII CONTENTS 14
IDENTIFICATION OF METABOLITE-RIBOSWITCH INTERACTIONS USING NUCLEOTIDE
ANALOG INTERFERENCE MAPPING AND SUPPRESSION 193 JULIANE K. SOUKUP AND
GARRETT A. SOUKUP 15 RNA-DEPENDENT RNA SWITCHES IN BACTERIA 207 TINA M.
HENKIN 16 PROBING MRNA STRUCTURE AND SRNA-MRNA INTERACTIONS IN BACTERIA
USING ENZYMES AND LEAD(II) 215 CLEMENT CHEVALIER, THOMAS GEISSMANN,
ANNE-CATHERINE HEIFER, AND PASCALE ROMBY 17 STRUCTURAL PROBING OF RNA
THERMOSENSORS 233 CLAUDE CHIARUTTINI, FREDERIC ALLEMAND, AND MATHIAS
SPRINGER 18 RIBOSOMAL INITIATION COMPLEXES PROBED BY TOEPRINTING AND
EFFECT OF TRANS-ACTING TRANSLATIONAL REGULATORS IN BACTERIA 247 PIERRE
FECHTER, CLEMENT CHEVALIER, GULNARA YUSUPOVA, MARAT YUSUPOV, PASCALE
ROMBY, AND STEFANO MARZI 19 ISOLATION AND CHARACTERIZATION OF THE HEAT
SHOCK RNA 1 (HSR1) 265 ILYA SHAMOVSKY AND EVGENY NUDLER 20 ANALYSIS OF
TRNA-DIRECTED TRANSCRIPTION ANTITERMINATION IN THE T BOX SYSTEM IN VIVO
281 TINA M. HENKIN 21 IN VITRO SELECTION OF CONFORMATIONAL PROBES FOR
RIBOSWITCHES 291 GIINTER MAYER AND MICHAEL FAMULOK 22 A GREEN
FLUORESCENT PROTEIN (GFP) BASED PLASMID SYSTEM TO STUDY
POST-TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION IN VIVO 301 JOHANNES H.
URBAN ANDJORG VOGEL 23 HIGH-THROUGHPUT SCREENS TO DISCOVER SYNTHETIC
RIBOSWITCHES 321 SEAN A. LYNCH, SHANA TOPP, AND JUSTIN P. GALLIVAN 24 A
MAMMALIAN CELL-BASED ASSAY FOR SCREENING INHIBITORS OF RNA CLEAVAGE 335
LAISING YEN, BRENT R. STOCKWELL, AND RICHARD C. MULLIGAN 25 IN VITRO
SELECTION OF JJLMS RIBOZYMES 349 KRISTIAN H. LINK AND RONALD R. BREAKER
INDEX 365
|
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| indexdate | 2024-07-09T21:40:35Z |
| institution | BVB |
| language | English |
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| physical | XI, 366 S. Ill., graph. Darst. 27 cm |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Riboswitches methods and protocols ed. by Alexander Serganov New York Humana 2009 XI, 366 S. Ill., graph. Darst. 27 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 540 Includes bibliographical references and index Predicting riboswitch regulation on a genomic scale / J.E. Barrick -- Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications / R. Rieder, C. Hobartner and R. Micura -- Application of fluorescent measurements for characterization of riboswitch-ligand interactions / B. Heppell ... [et al.] -- Transcriptional approaches to riboswitch studies / A. Mironov, V. Epshtein and E. Nudler -- Kinetics of riboswitch regulation studied by in vitro transcription / J.K. Wickiser -- Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / J.F. Lemay ... [et al.] -- Methods for analysis of ligand-induced RNA conformational changes / C.A. Brautigam, C.A. Wakeman and W.C. Winkler -- Monitoring RNA-ligand interactions using isothermal titration calorimetry / S.D. Gilbert and R.T. Batey -- Preparation and crystallization of riboswitch-ligand complexes / O. Pikovskaya ... [et al.] -- Crystallization of the glmS ribozyme-riboswitch / D.J. Klein and A.R. Ferre-D'Amare -- Riboswitch conformations revealed by small-angle X-ray scattering / J. Lipfert, D. Herschlag and S. Doniach -- Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches / J. Buck ... [et al.] -- Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / C.A. Wakeman and W.C. Winkler -- Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / J.K. Soukup and G.A. Soukup -- RNA-dependent RNA switches in bacteria / T.M. Henkin -- Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / C. Chevalier ... [et al.] -- Structural probing of RNA thermosensors / C. Chiaruttini, F. Allem and M. Springer -- Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / P. Fechter ... [et al.] -- Isolation and characterization of the heat shock RNA 1 / I. Shamovsky and E. Nudler -- Analysis of tRNA-directed transcription antitermination in the T box system in vivo / T.M. Henkin -- In vitro selection of conformational probes for riboswitches / G. Mayer and M. Famulok -- A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / J.H. Urban and J. Vogel -- High-throughput screens to discover synthetic riboswitches / S.A. Lynch, S. Topp and J.P. Gallivan -- A mammalian cell-based assay for screening inhibitors of RNA cleavage / L. Yen, B.R. Stockwell and R.C. Mulligan -- In vitro selection of glmS ribozymes / K.H. Link and R.R. Breaker RNA. RNA-protein interactions Messenger RNA. Ligands (Biochemistry) RNA / chemistry / Laboratory Manuals Gene Expression Regulation / physiology / Laboratory Manuals Laboratory Techniques and Procedures / Laboratory Manuals Gene Expression Regulation physiology Laboratory Manuals Laboratory Techniques and Procedures Laboratory Manuals Messenger RNA RNA RNA chemistry Laboratory Manuals Serganov, Alexander edt Methods in molecular biology 540 (DE-604)BV035362695 540 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017622084&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Riboswitches methods and protocols Methods in molecular biology RNA. RNA-protein interactions Messenger RNA. Ligands (Biochemistry) RNA / chemistry / Laboratory Manuals Gene Expression Regulation / physiology / Laboratory Manuals Laboratory Techniques and Procedures / Laboratory Manuals Gene Expression Regulation physiology Laboratory Manuals Laboratory Techniques and Procedures Laboratory Manuals Messenger RNA RNA RNA chemistry Laboratory Manuals |
| title | Riboswitches methods and protocols |
| title_auth | Riboswitches methods and protocols |
| title_exact_search | Riboswitches methods and protocols |
| title_full | Riboswitches methods and protocols ed. by Alexander Serganov |
| title_fullStr | Riboswitches methods and protocols ed. by Alexander Serganov |
| title_full_unstemmed | Riboswitches methods and protocols ed. by Alexander Serganov |
| title_short | Riboswitches |
| title_sort | riboswitches methods and protocols |
| title_sub | methods and protocols |
| topic | RNA. RNA-protein interactions Messenger RNA. Ligands (Biochemistry) RNA / chemistry / Laboratory Manuals Gene Expression Regulation / physiology / Laboratory Manuals Laboratory Techniques and Procedures / Laboratory Manuals Gene Expression Regulation physiology Laboratory Manuals Laboratory Techniques and Procedures Laboratory Manuals Messenger RNA RNA RNA chemistry Laboratory Manuals |
| topic_facet | RNA. RNA-protein interactions Messenger RNA. Ligands (Biochemistry) RNA / chemistry / Laboratory Manuals Gene Expression Regulation / physiology / Laboratory Manuals Laboratory Techniques and Procedures / Laboratory Manuals Gene Expression Regulation physiology Laboratory Manuals Laboratory Techniques and Procedures Laboratory Manuals Messenger RNA RNA RNA chemistry Laboratory Manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017622084&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT serganovalexander riboswitchesmethodsandprotocols |