Overview of blotting techniques /:
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Ashland Burlington, Ontario :
Delve Publishing,
2020.
|
| Schlagworte: | |
| Online-Zugang: | DE-862 DE-863 |
| Beschreibung: | Authors' Contributions. |
| Beschreibung: | 1 online resource (255 pages) |
| Bibliographie: | References -- Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract -- Abstract -- Introduction -- Preparation of Mab Against Natural Products -- Conclusions -- References -- Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Funding Statement -- References -- Chapter 8 The Design of A Quantitative Western Blot Experiment -- Abstract -- Introduction. |
| ISBN: | 1774078945 9781774078945 |
Internformat
MARC
| LEADER | 00000cam a22000001i 4500 | ||
|---|---|---|---|
| 001 | ZDB-4-EBA-on1231609943 | ||
| 003 | OCoLC | ||
| 005 | 20250103110447.0 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 210116s2020 xx o ||| 0 eng d | ||
| 040 | |a EBLCP |b eng |e rda |c EBLCP |d UKAHL |d N$T |d OCLCF |d OCLCO |d K6U |d OCLCQ |d OCLCO |d OCLCL |d A7U |d UKKRT | ||
| 020 | |a 1774078945 | ||
| 020 | |a 9781774078945 |q (electronic bk.) | ||
| 035 | |a (OCoLC)1231609943 | ||
| 050 | 4 | |a QP519.9.I43 | |
| 082 | 7 | |a 660.6 |2 23 | |
| 090 | |a NK1505 |b .O947 2021 | ||
| 049 | |a MAIN | ||
| 245 | 0 | 0 | |a Overview of blotting techniques / |c edited by Shiv Sanjeevi Sripathi and Prerna Pandey. |
| 264 | 1 | |a Ashland |a Burlington, Ontario : |b Delve Publishing, |c 2020. | |
| 264 | 4 | |c ©2021 | |
| 300 | |a 1 online resource (255 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 588 | |a Description based upon print version of record. | ||
| 505 | 0 | |a Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting | |
| 505 | 8 | |a 2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998) | |
| 505 | 8 | |a 3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments | |
| 504 | |a References -- Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract -- Abstract -- Introduction -- Preparation of Mab Against Natural Products -- Conclusions -- References -- Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Funding Statement -- References -- Chapter 8 The Design of A Quantitative Western Blot Experiment -- Abstract -- Introduction. | ||
| 505 | 8 | |a Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests | |
| 500 | |a Authors' Contributions. | ||
| 650 | 0 | |a Immunoblotting. |0 http://id.loc.gov/authorities/subjects/sh87004870 | |
| 650 | 0 | |a Western immunoblotting. |0 http://id.loc.gov/authorities/subjects/sh88007495 | |
| 650 | 0 | |a Macromolecules. |0 http://id.loc.gov/authorities/subjects/sh85079449 | |
| 650 | 6 | |a Technique des immunoempreintes. | |
| 650 | 6 | |a Buvardage de western. | |
| 650 | 7 | |a Immunoblotting |2 fast | |
| 650 | 7 | |a Macromolecules |2 fast | |
| 650 | 7 | |a Western immunoblotting |2 fast | |
| 700 | 1 | |a Sripathi, Shiv Sanjeevi, |e editor. | |
| 700 | 1 | |a Pandey, Prerna, |e editor. |0 http://id.loc.gov/authorities/names/n2018181363 | |
| 758 | |i has work: |a OVERVIEW OF BLOTTING TECHNIQUES (Text) |1 https://id.oclc.org/worldcat/entity/E39PD39vv9jq43FXTgrtKGxjvd |4 https://id.oclc.org/worldcat/ontology/hasWork | ||
| 776 | 0 | 8 | |i Print version: |a Sripathi, Shiv Sanjeevi |t Overview of blotting techniques |d Ashland : Delve Publishing,c2020 |
| 966 | 4 | 0 | |l DE-862 |p ZDB-4-EBA |q FWS_PDA_EBA |u https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2725292 |3 Volltext |
| 966 | 4 | 0 | |l DE-863 |p ZDB-4-EBA |q FWS_PDA_EBA |u https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2725292 |3 Volltext |
| 938 | |a Kortext |b KTXT |n 2248876 | ||
| 938 | |a Askews and Holts Library Services |b ASKH |n AH38250116 | ||
| 938 | |a ProQuest Ebook Central |b EBLB |n EBL6453533 | ||
| 938 | |a EBSCOhost |b EBSC |n 2725292 | ||
| 994 | |a 92 |b GEBAY | ||
| 912 | |a ZDB-4-EBA | ||
| 049 | |a DE-862 | ||
| 049 | |a DE-863 | ||
Datensatz im Suchindex
| DE-BY-FWS_katkey | ZDB-4-EBA-on1231609943 |
|---|---|
| _version_ | 1829095347654230016 |
| adam_text | |
| any_adam_object | |
| author2 | Sripathi, Shiv Sanjeevi Pandey, Prerna |
| author2_role | edt edt |
| author2_variant | s s s ss sss p p pp |
| author_GND | http://id.loc.gov/authorities/names/n2018181363 |
| author_facet | Sripathi, Shiv Sanjeevi Pandey, Prerna |
| building | Verbundindex |
| bvnumber | localFWS |
| callnumber-first | Q - Science |
| callnumber-label | QP519 |
| callnumber-raw | QP519.9.I43 NK1505 .O947 2021 |
| callnumber-search | QP519.9.I43 NK1505 .O947 2021 |
| callnumber-sort | QP 3519.9 I43 |
| callnumber-subject | QP - Physiology |
| collection | ZDB-4-EBA |
| contents | Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting 2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998) 3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests |
| ctrlnum | (OCoLC)1231609943 |
| dewey-full | 660.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6 |
| dewey-search | 660.6 |
| dewey-sort | 3660.6 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>05269cam a22005771i 4500</leader><controlfield tag="001">ZDB-4-EBA-on1231609943</controlfield><controlfield tag="003">OCoLC</controlfield><controlfield tag="005">20250103110447.0</controlfield><controlfield tag="006">m o d </controlfield><controlfield tag="007">cr cnu---unuuu</controlfield><controlfield tag="008">210116s2020 xx o ||| 0 eng d</controlfield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">EBLCP</subfield><subfield code="b">eng</subfield><subfield code="e">rda</subfield><subfield code="c">EBLCP</subfield><subfield code="d">UKAHL</subfield><subfield code="d">N$T</subfield><subfield code="d">OCLCF</subfield><subfield code="d">OCLCO</subfield><subfield code="d">K6U</subfield><subfield code="d">OCLCQ</subfield><subfield code="d">OCLCO</subfield><subfield code="d">OCLCL</subfield><subfield code="d">A7U</subfield><subfield code="d">UKKRT</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1774078945</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781774078945</subfield><subfield code="q">(electronic bk.)</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1231609943</subfield></datafield><datafield tag="050" ind1=" " ind2="4"><subfield code="a">QP519.9.I43</subfield></datafield><datafield tag="082" ind1="7" ind2=" "><subfield code="a">660.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="090" ind1=" " ind2=" "><subfield code="a">NK1505</subfield><subfield code="b">.O947 2021</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">MAIN</subfield></datafield><datafield tag="245" ind1="0" ind2="0"><subfield code="a">Overview of blotting techniques /</subfield><subfield code="c">edited by Shiv Sanjeevi Sripathi and Prerna Pandey.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Ashland</subfield><subfield code="a">Burlington, Ontario :</subfield><subfield code="b">Delve Publishing,</subfield><subfield code="c">2020.</subfield></datafield><datafield tag="264" ind1=" " ind2="4"><subfield code="c">©2021</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 online resource (255 pages)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">computer</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">online resource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="588" ind1=" " ind2=" "><subfield code="a">Description based upon print version of record.</subfield></datafield><datafield tag="505" ind1="0" ind2=" "><subfield code="a">Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998)</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments</subfield></datafield><datafield tag="504" ind1=" " ind2=" "><subfield code="a">References -- Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract -- Abstract -- Introduction -- Preparation of Mab Against Natural Products -- Conclusions -- References -- Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Funding Statement -- References -- Chapter 8 The Design of A Quantitative Western Blot Experiment -- Abstract -- Introduction.</subfield></datafield><datafield tag="505" ind1="8" ind2=" "><subfield code="a">Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Authors' Contributions.</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Immunoblotting.</subfield><subfield code="0">http://id.loc.gov/authorities/subjects/sh87004870</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Western immunoblotting.</subfield><subfield code="0">http://id.loc.gov/authorities/subjects/sh88007495</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Macromolecules.</subfield><subfield code="0">http://id.loc.gov/authorities/subjects/sh85079449</subfield></datafield><datafield tag="650" ind1=" " ind2="6"><subfield code="a">Technique des immunoempreintes.</subfield></datafield><datafield tag="650" ind1=" " ind2="6"><subfield code="a">Buvardage de western.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immunoblotting</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Macromolecules</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Western immunoblotting</subfield><subfield code="2">fast</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sripathi, Shiv Sanjeevi,</subfield><subfield code="e">editor.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pandey, Prerna,</subfield><subfield code="e">editor.</subfield><subfield code="0">http://id.loc.gov/authorities/names/n2018181363</subfield></datafield><datafield tag="758" ind1=" " ind2=" "><subfield code="i">has work:</subfield><subfield code="a">OVERVIEW OF BLOTTING TECHNIQUES (Text)</subfield><subfield code="1">https://id.oclc.org/worldcat/entity/E39PD39vv9jq43FXTgrtKGxjvd</subfield><subfield code="4">https://id.oclc.org/worldcat/ontology/hasWork</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Print version:</subfield><subfield code="a">Sripathi, Shiv Sanjeevi</subfield><subfield code="t">Overview of blotting techniques</subfield><subfield code="d">Ashland : Delve Publishing,c2020</subfield></datafield><datafield tag="966" ind1="4" ind2="0"><subfield code="l">DE-862</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FWS_PDA_EBA</subfield><subfield code="u">https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2725292</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="4" ind2="0"><subfield code="l">DE-863</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FWS_PDA_EBA</subfield><subfield code="u">https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=2725292</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">Kortext</subfield><subfield code="b">KTXT</subfield><subfield code="n">2248876</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">Askews and Holts Library Services</subfield><subfield code="b">ASKH</subfield><subfield code="n">AH38250116</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">ProQuest Ebook Central</subfield><subfield code="b">EBLB</subfield><subfield code="n">EBL6453533</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">EBSCOhost</subfield><subfield code="b">EBSC</subfield><subfield code="n">2725292</subfield></datafield><datafield tag="994" ind1=" " ind2=" "><subfield code="a">92</subfield><subfield code="b">GEBAY</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-4-EBA</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-862</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-863</subfield></datafield></record></collection> |
| id | ZDB-4-EBA-on1231609943 |
| illustrated | Not Illustrated |
| indexdate | 2025-04-11T08:47:35Z |
| institution | BVB |
| isbn | 1774078945 9781774078945 |
| language | English |
| oclc_num | 1231609943 |
| open_access_boolean | |
| owner | MAIN DE-862 DE-BY-FWS DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-862 DE-BY-FWS DE-863 DE-BY-FWS |
| physical | 1 online resource (255 pages) |
| psigel | ZDB-4-EBA FWS_PDA_EBA ZDB-4-EBA |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | Delve Publishing, |
| record_format | marc |
| spelling | Overview of blotting techniques / edited by Shiv Sanjeevi Sripathi and Prerna Pandey. Ashland Burlington, Ontario : Delve Publishing, 2020. ©2021 1 online resource (255 pages) text txt rdacontent computer c rdamedia online resource cr rdacarrier Description based upon print version of record. Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting 2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998) 3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments References -- Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract -- Abstract -- Introduction -- Preparation of Mab Against Natural Products -- Conclusions -- References -- Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Funding Statement -- References -- Chapter 8 The Design of A Quantitative Western Blot Experiment -- Abstract -- Introduction. Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests Authors' Contributions. Immunoblotting. http://id.loc.gov/authorities/subjects/sh87004870 Western immunoblotting. http://id.loc.gov/authorities/subjects/sh88007495 Macromolecules. http://id.loc.gov/authorities/subjects/sh85079449 Technique des immunoempreintes. Buvardage de western. Immunoblotting fast Macromolecules fast Western immunoblotting fast Sripathi, Shiv Sanjeevi, editor. Pandey, Prerna, editor. http://id.loc.gov/authorities/names/n2018181363 has work: OVERVIEW OF BLOTTING TECHNIQUES (Text) https://id.oclc.org/worldcat/entity/E39PD39vv9jq43FXTgrtKGxjvd https://id.oclc.org/worldcat/ontology/hasWork Print version: Sripathi, Shiv Sanjeevi Overview of blotting techniques Ashland : Delve Publishing,c2020 |
| spellingShingle | Overview of blotting techniques / Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting 2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998) 3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests Immunoblotting. http://id.loc.gov/authorities/subjects/sh87004870 Western immunoblotting. http://id.loc.gov/authorities/subjects/sh88007495 Macromolecules. http://id.loc.gov/authorities/subjects/sh85079449 Technique des immunoempreintes. Buvardage de western. Immunoblotting fast Macromolecules fast Western immunoblotting fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh87004870 http://id.loc.gov/authorities/subjects/sh88007495 http://id.loc.gov/authorities/subjects/sh85079449 |
| title | Overview of blotting techniques / |
| title_auth | Overview of blotting techniques / |
| title_exact_search | Overview of blotting techniques / |
| title_full | Overview of blotting techniques / edited by Shiv Sanjeevi Sripathi and Prerna Pandey. |
| title_fullStr | Overview of blotting techniques / edited by Shiv Sanjeevi Sripathi and Prerna Pandey. |
| title_full_unstemmed | Overview of blotting techniques / edited by Shiv Sanjeevi Sripathi and Prerna Pandey. |
| title_short | Overview of blotting techniques / |
| title_sort | overview of blotting techniques |
| topic | Immunoblotting. http://id.loc.gov/authorities/subjects/sh87004870 Western immunoblotting. http://id.loc.gov/authorities/subjects/sh88007495 Macromolecules. http://id.loc.gov/authorities/subjects/sh85079449 Technique des immunoempreintes. Buvardage de western. Immunoblotting fast Macromolecules fast Western immunoblotting fast |
| topic_facet | Immunoblotting. Western immunoblotting. Macromolecules. Technique des immunoempreintes. Buvardage de western. Immunoblotting Macromolecules Western immunoblotting |
| work_keys_str_mv | AT sripathishivsanjeevi overviewofblottingtechniques AT pandeyprerna overviewofblottingtechniques |