Multiplexing of PCR assays in breast cancer analysis:
HauptbeschreibungDas Werk beschreibt die Optimierung eines prognostischen molekularbiologischen Tests für die Brustkrebs-Diagnose und -Therapieentscheidung. Im Rahmen der Arbeit wurde die Zahl der Reaktionsräume für Polymerasekettenreaktionen (PCR) durch die Optimierung vom Multiplexen derart reduzi...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
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Hamburg :
Bachelor + Master Pub.,
2012.
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| Online-Zugang: | Volltext |
| Zusammenfassung: | HauptbeschreibungDas Werk beschreibt die Optimierung eines prognostischen molekularbiologischen Tests für die Brustkrebs-Diagnose und -Therapieentscheidung. Im Rahmen der Arbeit wurde die Zahl der Reaktionsräume für Polymerasekettenreaktionen (PCR) durch die Optimierung vom Multiplexen derart reduziert, dass parallele und kosteneffizientere Analysen möglich wurden. Biographische InformationenPatrick Maaß, B.Sc., wurde 1973 in Wattenscheid geboren. Nach einer Ausbildung zum Biologielaboranten bei einem großen deutschen Pharmaforschungsunternehmen entschied sich der. |
| Beschreibung: | Description based upon print version of record. |
| Beschreibung: | 1 online resource (54 p.) |
| Bibliographie: | Includes bibliographical references. |
| ISBN: | 3863416074 9783863416072 |
Internformat
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| 505 | 0 | |a Multiplexing of PCR Assays in Breast Cancer Analysis; Table of Contents; 1 Introduction; 1.1 Breast Cancer; 1.2 RNA Expression Profiling and Prediction; 1.3 Objectives; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments and Consumables; 2.1.2 Buffers and Chemicals for Nucleic Acid Purification; 2.1.3 Nucleic Acids, Cell Lines & Tissue Samples; 2.1.4 Synthetic Oligonucleotides; 2.1.5 Chemicals and Kits for PCR; 2.1.6 Software; 2.2 Methods; 2.2.1 General Approach; 2.2.2 Purification of Nucleic Acids; 2.2.3 Creation of a Standard Reference RNA Pool; 2.2.4 Real-Time Kinetic RT-PCR | |
| 505 | 8 | |a 2.2.5 Designing Dually Labeled Primer-Probe Sets2.2.6 Dilution of Primer-Probe Sets; 2.2.7 Setup of Singleplex kPCR Assays; 2.2.8 Evaluation of Different Reporter Dyes; 2.2.9 Controlling of Primer-Probe-Set Performance; 2.2.10 Testing Combinations of Two Primer-Probe Sets (Duplexing); 2.2.11 Testing Combinations of Three Sets (Triplexing); 3 Results; 3.1 Identification of Suitable Reporter Dyes; 3.2 Evaluation of Primer-Probe-Performance; 3.3 Identification of Suitable Duplex Combinations; 3.4 Identification of Suitable Triplex Combinations; 4 Discussion | |
| 505 | 8 | |a 4.1 Statistical Evaluation of Primer Performances4.1.1 Slope; 4.1.2 Efficiency; 4.1.3 Y-Intercept; 4.2 Suitable Reporter Dyes and Quencher; 4.3 Optimization of Assays and kinetic RT-PCR Parameter; 4.4 Conclusion and Outlook; 5 Summary; 6 Literature; 7 Appendix; 7.1 Abbreviations; 7.2 Figures; 7.3 Tables; 7.4 Oligonucleotide Sequences; 7.5 Origins of Breast Cancer Samples for MAVPOOL080623a; 7.6 Acknowledgements | |
| 520 | |a HauptbeschreibungDas Werk beschreibt die Optimierung eines prognostischen molekularbiologischen Tests für die Brustkrebs-Diagnose und -Therapieentscheidung. Im Rahmen der Arbeit wurde die Zahl der Reaktionsräume für Polymerasekettenreaktionen (PCR) durch die Optimierung vom Multiplexen derart reduziert, dass parallele und kosteneffizientere Analysen möglich wurden. Biographische InformationenPatrick Maaß, B.Sc., wurde 1973 in Wattenscheid geboren. Nach einer Ausbildung zum Biologielaboranten bei einem großen deutschen Pharmaforschungsunternehmen entschied sich der. | ||
| 546 | |a English. | ||
| 504 | |a Includes bibliographical references. | ||
| 650 | 0 | |a Breast |x Cancer |x Diagnosis. | |
| 650 | 0 | |a Breast |x Cancer |x Imaging. | |
| 650 | 6 | |a Sein |x Cancer |x Imagerie. | |
| 650 | 7 | |a Breast |x Cancer |x Diagnosis |2 fast | |
| 650 | 7 | |a Breast |x Cancer |x Imaging |2 fast | |
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| _version_ | 1816882523618148352 |
| adam_text | |
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| author | Maass, Patrick |
| author_facet | Maass, Patrick |
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| callnumber-raw | RC280.B8 M33 2012 |
| callnumber-search | RC280.B8 M33 2012 |
| callnumber-sort | RC 3280 B8 M33 42012 |
| callnumber-subject | RC - Internal Medicine |
| collection | ZDB-4-EBA |
| contents | Multiplexing of PCR Assays in Breast Cancer Analysis; Table of Contents; 1 Introduction; 1.1 Breast Cancer; 1.2 RNA Expression Profiling and Prediction; 1.3 Objectives; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments and Consumables; 2.1.2 Buffers and Chemicals for Nucleic Acid Purification; 2.1.3 Nucleic Acids, Cell Lines & Tissue Samples; 2.1.4 Synthetic Oligonucleotides; 2.1.5 Chemicals and Kits for PCR; 2.1.6 Software; 2.2 Methods; 2.2.1 General Approach; 2.2.2 Purification of Nucleic Acids; 2.2.3 Creation of a Standard Reference RNA Pool; 2.2.4 Real-Time Kinetic RT-PCR 2.2.5 Designing Dually Labeled Primer-Probe Sets2.2.6 Dilution of Primer-Probe Sets; 2.2.7 Setup of Singleplex kPCR Assays; 2.2.8 Evaluation of Different Reporter Dyes; 2.2.9 Controlling of Primer-Probe-Set Performance; 2.2.10 Testing Combinations of Two Primer-Probe Sets (Duplexing); 2.2.11 Testing Combinations of Three Sets (Triplexing); 3 Results; 3.1 Identification of Suitable Reporter Dyes; 3.2 Evaluation of Primer-Probe-Performance; 3.3 Identification of Suitable Duplex Combinations; 3.4 Identification of Suitable Triplex Combinations; 4 Discussion 4.1 Statistical Evaluation of Primer Performances4.1.1 Slope; 4.1.2 Efficiency; 4.1.3 Y-Intercept; 4.2 Suitable Reporter Dyes and Quencher; 4.3 Optimization of Assays and kinetic RT-PCR Parameter; 4.4 Conclusion and Outlook; 5 Summary; 6 Literature; 7 Appendix; 7.1 Abbreviations; 7.2 Figures; 7.3 Tables; 7.4 Oligonucleotide Sequences; 7.5 Origins of Breast Cancer Samples for MAVPOOL080623a; 7.6 Acknowledgements |
| ctrlnum | (OCoLC)1162373032 |
| dewey-full | 616.99449 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.99449 |
| dewey-search | 616.99449 |
| dewey-sort | 3616.99449 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| format | Electronic eBook |
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| id | ZDB-4-EBA-on1162373032 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:29:57Z |
| institution | BVB |
| isbn | 3863416074 9783863416072 |
| language | English |
| oclc_num | 1162373032 |
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| physical | 1 online resource (54 p.) |
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| publishDate | 2012 |
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| publisher | Bachelor + Master Pub., |
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| spelling | Maass, Patrick. Multiplexing of PCR assays in breast cancer analysis [electronic resource] / Patrick Maass. Hamburg : Bachelor + Master Pub., 2012. 1 online resource (54 p.) text txt computer c online resource cr Description based upon print version of record. Multiplexing of PCR Assays in Breast Cancer Analysis; Table of Contents; 1 Introduction; 1.1 Breast Cancer; 1.2 RNA Expression Profiling and Prediction; 1.3 Objectives; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments and Consumables; 2.1.2 Buffers and Chemicals for Nucleic Acid Purification; 2.1.3 Nucleic Acids, Cell Lines & Tissue Samples; 2.1.4 Synthetic Oligonucleotides; 2.1.5 Chemicals and Kits for PCR; 2.1.6 Software; 2.2 Methods; 2.2.1 General Approach; 2.2.2 Purification of Nucleic Acids; 2.2.3 Creation of a Standard Reference RNA Pool; 2.2.4 Real-Time Kinetic RT-PCR 2.2.5 Designing Dually Labeled Primer-Probe Sets2.2.6 Dilution of Primer-Probe Sets; 2.2.7 Setup of Singleplex kPCR Assays; 2.2.8 Evaluation of Different Reporter Dyes; 2.2.9 Controlling of Primer-Probe-Set Performance; 2.2.10 Testing Combinations of Two Primer-Probe Sets (Duplexing); 2.2.11 Testing Combinations of Three Sets (Triplexing); 3 Results; 3.1 Identification of Suitable Reporter Dyes; 3.2 Evaluation of Primer-Probe-Performance; 3.3 Identification of Suitable Duplex Combinations; 3.4 Identification of Suitable Triplex Combinations; 4 Discussion 4.1 Statistical Evaluation of Primer Performances4.1.1 Slope; 4.1.2 Efficiency; 4.1.3 Y-Intercept; 4.2 Suitable Reporter Dyes and Quencher; 4.3 Optimization of Assays and kinetic RT-PCR Parameter; 4.4 Conclusion and Outlook; 5 Summary; 6 Literature; 7 Appendix; 7.1 Abbreviations; 7.2 Figures; 7.3 Tables; 7.4 Oligonucleotide Sequences; 7.5 Origins of Breast Cancer Samples for MAVPOOL080623a; 7.6 Acknowledgements HauptbeschreibungDas Werk beschreibt die Optimierung eines prognostischen molekularbiologischen Tests für die Brustkrebs-Diagnose und -Therapieentscheidung. Im Rahmen der Arbeit wurde die Zahl der Reaktionsräume für Polymerasekettenreaktionen (PCR) durch die Optimierung vom Multiplexen derart reduziert, dass parallele und kosteneffizientere Analysen möglich wurden. Biographische InformationenPatrick Maaß, B.Sc., wurde 1973 in Wattenscheid geboren. Nach einer Ausbildung zum Biologielaboranten bei einem großen deutschen Pharmaforschungsunternehmen entschied sich der. English. Includes bibliographical references. Breast Cancer Diagnosis. Breast Cancer Imaging. Sein Cancer Imagerie. Breast Cancer Diagnosis fast Breast Cancer Imaging fast Print version: 3863411072 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=1087597 Volltext |
| spellingShingle | Maass, Patrick Multiplexing of PCR assays in breast cancer analysis Multiplexing of PCR Assays in Breast Cancer Analysis; Table of Contents; 1 Introduction; 1.1 Breast Cancer; 1.2 RNA Expression Profiling and Prediction; 1.3 Objectives; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments and Consumables; 2.1.2 Buffers and Chemicals for Nucleic Acid Purification; 2.1.3 Nucleic Acids, Cell Lines & Tissue Samples; 2.1.4 Synthetic Oligonucleotides; 2.1.5 Chemicals and Kits for PCR; 2.1.6 Software; 2.2 Methods; 2.2.1 General Approach; 2.2.2 Purification of Nucleic Acids; 2.2.3 Creation of a Standard Reference RNA Pool; 2.2.4 Real-Time Kinetic RT-PCR 2.2.5 Designing Dually Labeled Primer-Probe Sets2.2.6 Dilution of Primer-Probe Sets; 2.2.7 Setup of Singleplex kPCR Assays; 2.2.8 Evaluation of Different Reporter Dyes; 2.2.9 Controlling of Primer-Probe-Set Performance; 2.2.10 Testing Combinations of Two Primer-Probe Sets (Duplexing); 2.2.11 Testing Combinations of Three Sets (Triplexing); 3 Results; 3.1 Identification of Suitable Reporter Dyes; 3.2 Evaluation of Primer-Probe-Performance; 3.3 Identification of Suitable Duplex Combinations; 3.4 Identification of Suitable Triplex Combinations; 4 Discussion 4.1 Statistical Evaluation of Primer Performances4.1.1 Slope; 4.1.2 Efficiency; 4.1.3 Y-Intercept; 4.2 Suitable Reporter Dyes and Quencher; 4.3 Optimization of Assays and kinetic RT-PCR Parameter; 4.4 Conclusion and Outlook; 5 Summary; 6 Literature; 7 Appendix; 7.1 Abbreviations; 7.2 Figures; 7.3 Tables; 7.4 Oligonucleotide Sequences; 7.5 Origins of Breast Cancer Samples for MAVPOOL080623a; 7.6 Acknowledgements Breast Cancer Diagnosis. Breast Cancer Imaging. Sein Cancer Imagerie. Breast Cancer Diagnosis fast Breast Cancer Imaging fast |
| title | Multiplexing of PCR assays in breast cancer analysis |
| title_auth | Multiplexing of PCR assays in breast cancer analysis |
| title_exact_search | Multiplexing of PCR assays in breast cancer analysis |
| title_full | Multiplexing of PCR assays in breast cancer analysis [electronic resource] / Patrick Maass. |
| title_fullStr | Multiplexing of PCR assays in breast cancer analysis [electronic resource] / Patrick Maass. |
| title_full_unstemmed | Multiplexing of PCR assays in breast cancer analysis [electronic resource] / Patrick Maass. |
| title_short | Multiplexing of PCR assays in breast cancer analysis |
| title_sort | multiplexing of pcr assays in breast cancer analysis |
| topic | Breast Cancer Diagnosis. Breast Cancer Imaging. Sein Cancer Imagerie. Breast Cancer Diagnosis fast Breast Cancer Imaging fast |
| topic_facet | Breast Cancer Diagnosis. Breast Cancer Imaging. Sein Cancer Imagerie. Breast Cancer Diagnosis Breast Cancer Imaging |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=1087597 |
| work_keys_str_mv | AT maasspatrick multiplexingofpcrassaysinbreastcanceranalysis |