Metagenomics :: methods, applications and perspectives /
Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York :
Nova Publishers,
[2014]
|
| Schriftenreihe: | Microbiology research advances series.
|
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Zusammenfassung: | Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a particular niche rather than a laboratory culture. Thus, metagenomic analyses potentially allow access to all the genetic resources present in an environment, regardless of whether or not they belong to microorganisms that can be cultured in the laboratory. Sequence-based metagenomic analyses rely. |
| Beschreibung: | 1 online resource |
| Bibliographie: | Includes bibliographical references. |
| ISBN: | 9781633216983 1633216985 |
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| 505 | 0 | |a METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; Chapter 1: POTENTIAL AND LIMITATIONS OF METAGENOMIC FUNCTIONAL ANALYSES; ABSTRACT; 1. MICROBIAL DIVERSITY; 2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION; 3. METAGENOMIC LIBRARY ANALYSES; 4. FUNCTIONAL SCREENS; 5. METAGENOMIC GENE EXPRESION; 6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS; REFERENCES; Chapter 2: METAGENOMICS: LIBRARY CONSTRUCTION AND SCREENING METHODS; ABSTRACT; 1. INTRODUCTION. | |
| 505 | 8 | |a 2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES3. ENRICHMENT OF ENVIRONMENTAL SAMPLES; 4. CONSTRUCTION OF A METAGENOMIC LIBRARY; 5. SCREENING OF METAGENOMIC DATA; CONCLUSION; REFERENCES; Chapter 3: THE USE OF ION TORRENT PGM FOR BACTERIAL DIVERSITY ANALYSES: THE STUDY CASE OF FIVE BRAZILIAN HYDROELECTRIC RESERVOIRS; ABSTRACT; INTRODUCTION; CONCLUSION; REFERENCES; Chapter 4: METAGENOMIC PROFILING FOR ASSESSING ENVIRONMENTAL HEALTHY; ABSTRACT; 1. INTRODUCTION; 2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS; 3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH; REFERENCES. | |
| 505 | 8 | |a Chapter 5: MINING NOVEL GENES AND ENZYMES OF UNCULTURED EUKARYOTIC MICROORGANISMS BY AN RNA-BASED APPROACHABSTRACT; METAGENOMICS: ACCESS TO THE UNCULTURED MICROBIAL RESOURCES; TARGET OF METAGENOMIC ANALYSIS; APPROACHES TO METAGENOMIC SCREENING; EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS; EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH; CONCLUDING REMARKS AND FUTURE PROSPECTS; REFERENCES; Chapter 6: DEVELOPMENTS IN THE RETRIEVAL OF NOVEL BIOCATALYSTS BY METAGENOMIC APPROACHES; ABSTRACT; INTRODUCTION. | |
| 505 | 8 | |a OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNAPROBLEMS IN SCREENING METAGENOMIC LIBRARIES; PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS; OTHER INDUSTRIALLY IMPORTANT ENZYMES; SUCCESS STORIES AND COMMERCIALIZED METAGENOMIC BIOCATALYSTS; CONCLUSION; REFERENCES; Chapter 7: ISOLATION OF SOIL METAGENOMIC DNA: CHALLENGES AND SOLUTIONS; ABSTRACT; INTRODUCTION; 1. ORIGIN OF METAGENOMICS; 2. SOIL -- THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES; 3. ISOLATION OF METAGENOMIC DNA; CONCLUSION; REFERENCES. | |
| 505 | 8 | |a Chapter 8: MICROBIAL EXPLORATION IN EXTREMECONDITIONS: METAGENOMIC ANALYSISAND FUTURE PERSPECTIVESABSTRACT; INTRODUCTION; LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS; SANGER AND NEXT GENERATION SEQUENCING; THE METAGENOMICS PIPELINE; BIOINFORMATICS AND THERMOPHILE METAGENOMICS; ANNOTATION OF METAGENOMIC SEQUENCES; DIFFERENT METAGENOMIC APPROACHES FOR MICROBIOLOGY; SEQUENCE-DRIVEN METAGENOMICS; FUNCTION-DRIVEN METAGENOMICS; EXTREME ECOLOGICAL ENVIRONMENTS; HOT WATER SPRINGS; THERMOPHILIC MICROORGANISMS IN HOT SPRINGS; THERMOPHILIC HOT SPRINGS BACTERIA; THERMOPHILIC VIRUSES. | |
| 520 | |a Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a particular niche rather than a laboratory culture. Thus, metagenomic analyses potentially allow access to all the genetic resources present in an environment, regardless of whether or not they belong to microorganisms that can be cultured in the laboratory. Sequence-based metagenomic analyses rely. | ||
| 504 | |a Includes bibliographical references. | ||
| 650 | 0 | |a Microbial genomics. |0 http://id.loc.gov/authorities/subjects/sh2003001301 | |
| 650 | 6 | |a Génomique microbienne. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Biochemistry. |2 bisacsh | |
| 650 | 7 | |a Microbial genomics |2 fast | |
| 700 | 1 | |a Benedetti, Camilla, |e editor. | |
| 776 | 0 | 8 | |i Print version: |a Benedetti, Camilla. |t Metagenomics : Methods, Applications and Perspectives. |d Hauppauge : Nova Science Publishers, Inc., ©2014 |z 9781611223583 |
| 830 | 0 | |a Microbiology research advances series. |0 http://id.loc.gov/authorities/names/n2010182318 | |
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Datensatz im Suchindex
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| _version_ | 1816882290444206080 |
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| contents | METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; Chapter 1: POTENTIAL AND LIMITATIONS OF METAGENOMIC FUNCTIONAL ANALYSES; ABSTRACT; 1. MICROBIAL DIVERSITY; 2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION; 3. METAGENOMIC LIBRARY ANALYSES; 4. FUNCTIONAL SCREENS; 5. METAGENOMIC GENE EXPRESION; 6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS; REFERENCES; Chapter 2: METAGENOMICS: LIBRARY CONSTRUCTION AND SCREENING METHODS; ABSTRACT; 1. INTRODUCTION. 2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES3. ENRICHMENT OF ENVIRONMENTAL SAMPLES; 4. CONSTRUCTION OF A METAGENOMIC LIBRARY; 5. SCREENING OF METAGENOMIC DATA; CONCLUSION; REFERENCES; Chapter 3: THE USE OF ION TORRENT PGM FOR BACTERIAL DIVERSITY ANALYSES: THE STUDY CASE OF FIVE BRAZILIAN HYDROELECTRIC RESERVOIRS; ABSTRACT; INTRODUCTION; CONCLUSION; REFERENCES; Chapter 4: METAGENOMIC PROFILING FOR ASSESSING ENVIRONMENTAL HEALTHY; ABSTRACT; 1. INTRODUCTION; 2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS; 3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH; REFERENCES. Chapter 5: MINING NOVEL GENES AND ENZYMES OF UNCULTURED EUKARYOTIC MICROORGANISMS BY AN RNA-BASED APPROACHABSTRACT; METAGENOMICS: ACCESS TO THE UNCULTURED MICROBIAL RESOURCES; TARGET OF METAGENOMIC ANALYSIS; APPROACHES TO METAGENOMIC SCREENING; EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS; EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH; CONCLUDING REMARKS AND FUTURE PROSPECTS; REFERENCES; Chapter 6: DEVELOPMENTS IN THE RETRIEVAL OF NOVEL BIOCATALYSTS BY METAGENOMIC APPROACHES; ABSTRACT; INTRODUCTION. OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNAPROBLEMS IN SCREENING METAGENOMIC LIBRARIES; PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS; OTHER INDUSTRIALLY IMPORTANT ENZYMES; SUCCESS STORIES AND COMMERCIALIZED METAGENOMIC BIOCATALYSTS; CONCLUSION; REFERENCES; Chapter 7: ISOLATION OF SOIL METAGENOMIC DNA: CHALLENGES AND SOLUTIONS; ABSTRACT; INTRODUCTION; 1. ORIGIN OF METAGENOMICS; 2. SOIL -- THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES; 3. ISOLATION OF METAGENOMIC DNA; CONCLUSION; REFERENCES. Chapter 8: MICROBIAL EXPLORATION IN EXTREMECONDITIONS: METAGENOMIC ANALYSISAND FUTURE PERSPECTIVESABSTRACT; INTRODUCTION; LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS; SANGER AND NEXT GENERATION SEQUENCING; THE METAGENOMICS PIPELINE; BIOINFORMATICS AND THERMOPHILE METAGENOMICS; ANNOTATION OF METAGENOMIC SEQUENCES; DIFFERENT METAGENOMIC APPROACHES FOR MICROBIOLOGY; SEQUENCE-DRIVEN METAGENOMICS; FUNCTION-DRIVEN METAGENOMICS; EXTREME ECOLOGICAL ENVIRONMENTS; HOT WATER SPRINGS; THERMOPHILIC MICROORGANISMS IN HOT SPRINGS; THERMOPHILIC HOT SPRINGS BACTERIA; THERMOPHILIC VIRUSES. |
| ctrlnum | (OCoLC)893208465 |
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| id | ZDB-4-EBA-ocn893208465 |
| illustrated | Not Illustrated |
| indexdate | 2024-11-27T13:26:16Z |
| institution | BVB |
| isbn | 9781633216983 1633216985 |
| language | English |
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| series | Microbiology research advances series. |
| series2 | Microbiology research advances |
| spelling | Metagenomics : methods, applications and perspectives / Camilla Benedetti, editor. New York : Nova Publishers, [2014] ©2014 1 online resource text txt rdacontent computer c rdamedia online resource cr rdacarrier Microbiology research advances Online resource; title from PDF title page (EBSCO, viewed October 17, 2014). METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; Chapter 1: POTENTIAL AND LIMITATIONS OF METAGENOMIC FUNCTIONAL ANALYSES; ABSTRACT; 1. MICROBIAL DIVERSITY; 2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION; 3. METAGENOMIC LIBRARY ANALYSES; 4. FUNCTIONAL SCREENS; 5. METAGENOMIC GENE EXPRESION; 6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS; REFERENCES; Chapter 2: METAGENOMICS: LIBRARY CONSTRUCTION AND SCREENING METHODS; ABSTRACT; 1. INTRODUCTION. 2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES3. ENRICHMENT OF ENVIRONMENTAL SAMPLES; 4. CONSTRUCTION OF A METAGENOMIC LIBRARY; 5. SCREENING OF METAGENOMIC DATA; CONCLUSION; REFERENCES; Chapter 3: THE USE OF ION TORRENT PGM FOR BACTERIAL DIVERSITY ANALYSES: THE STUDY CASE OF FIVE BRAZILIAN HYDROELECTRIC RESERVOIRS; ABSTRACT; INTRODUCTION; CONCLUSION; REFERENCES; Chapter 4: METAGENOMIC PROFILING FOR ASSESSING ENVIRONMENTAL HEALTHY; ABSTRACT; 1. INTRODUCTION; 2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS; 3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH; REFERENCES. Chapter 5: MINING NOVEL GENES AND ENZYMES OF UNCULTURED EUKARYOTIC MICROORGANISMS BY AN RNA-BASED APPROACHABSTRACT; METAGENOMICS: ACCESS TO THE UNCULTURED MICROBIAL RESOURCES; TARGET OF METAGENOMIC ANALYSIS; APPROACHES TO METAGENOMIC SCREENING; EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS; EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH; CONCLUDING REMARKS AND FUTURE PROSPECTS; REFERENCES; Chapter 6: DEVELOPMENTS IN THE RETRIEVAL OF NOVEL BIOCATALYSTS BY METAGENOMIC APPROACHES; ABSTRACT; INTRODUCTION. OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNAPROBLEMS IN SCREENING METAGENOMIC LIBRARIES; PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS; OTHER INDUSTRIALLY IMPORTANT ENZYMES; SUCCESS STORIES AND COMMERCIALIZED METAGENOMIC BIOCATALYSTS; CONCLUSION; REFERENCES; Chapter 7: ISOLATION OF SOIL METAGENOMIC DNA: CHALLENGES AND SOLUTIONS; ABSTRACT; INTRODUCTION; 1. ORIGIN OF METAGENOMICS; 2. SOIL -- THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES; 3. ISOLATION OF METAGENOMIC DNA; CONCLUSION; REFERENCES. Chapter 8: MICROBIAL EXPLORATION IN EXTREMECONDITIONS: METAGENOMIC ANALYSISAND FUTURE PERSPECTIVESABSTRACT; INTRODUCTION; LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS; SANGER AND NEXT GENERATION SEQUENCING; THE METAGENOMICS PIPELINE; BIOINFORMATICS AND THERMOPHILE METAGENOMICS; ANNOTATION OF METAGENOMIC SEQUENCES; DIFFERENT METAGENOMIC APPROACHES FOR MICROBIOLOGY; SEQUENCE-DRIVEN METAGENOMICS; FUNCTION-DRIVEN METAGENOMICS; EXTREME ECOLOGICAL ENVIRONMENTS; HOT WATER SPRINGS; THERMOPHILIC MICROORGANISMS IN HOT SPRINGS; THERMOPHILIC HOT SPRINGS BACTERIA; THERMOPHILIC VIRUSES. Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a particular niche rather than a laboratory culture. Thus, metagenomic analyses potentially allow access to all the genetic resources present in an environment, regardless of whether or not they belong to microorganisms that can be cultured in the laboratory. Sequence-based metagenomic analyses rely. Includes bibliographical references. Microbial genomics. http://id.loc.gov/authorities/subjects/sh2003001301 Génomique microbienne. SCIENCE Life Sciences Biochemistry. bisacsh Microbial genomics fast Benedetti, Camilla, editor. Print version: Benedetti, Camilla. Metagenomics : Methods, Applications and Perspectives. Hauppauge : Nova Science Publishers, Inc., ©2014 9781611223583 Microbiology research advances series. http://id.loc.gov/authorities/names/n2010182318 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=863028 Volltext |
| spellingShingle | Metagenomics : methods, applications and perspectives / Microbiology research advances series. METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES; Library of Congress Cataloging-in-Publication Data; CONTENTS; PREFACE; Chapter 1: POTENTIAL AND LIMITATIONS OF METAGENOMIC FUNCTIONAL ANALYSES; ABSTRACT; 1. MICROBIAL DIVERSITY; 2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION; 3. METAGENOMIC LIBRARY ANALYSES; 4. FUNCTIONAL SCREENS; 5. METAGENOMIC GENE EXPRESION; 6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS; REFERENCES; Chapter 2: METAGENOMICS: LIBRARY CONSTRUCTION AND SCREENING METHODS; ABSTRACT; 1. INTRODUCTION. 2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES3. ENRICHMENT OF ENVIRONMENTAL SAMPLES; 4. CONSTRUCTION OF A METAGENOMIC LIBRARY; 5. SCREENING OF METAGENOMIC DATA; CONCLUSION; REFERENCES; Chapter 3: THE USE OF ION TORRENT PGM FOR BACTERIAL DIVERSITY ANALYSES: THE STUDY CASE OF FIVE BRAZILIAN HYDROELECTRIC RESERVOIRS; ABSTRACT; INTRODUCTION; CONCLUSION; REFERENCES; Chapter 4: METAGENOMIC PROFILING FOR ASSESSING ENVIRONMENTAL HEALTHY; ABSTRACT; 1. INTRODUCTION; 2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS; 3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH; REFERENCES. Chapter 5: MINING NOVEL GENES AND ENZYMES OF UNCULTURED EUKARYOTIC MICROORGANISMS BY AN RNA-BASED APPROACHABSTRACT; METAGENOMICS: ACCESS TO THE UNCULTURED MICROBIAL RESOURCES; TARGET OF METAGENOMIC ANALYSIS; APPROACHES TO METAGENOMIC SCREENING; EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS; EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH; CONCLUDING REMARKS AND FUTURE PROSPECTS; REFERENCES; Chapter 6: DEVELOPMENTS IN THE RETRIEVAL OF NOVEL BIOCATALYSTS BY METAGENOMIC APPROACHES; ABSTRACT; INTRODUCTION. OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNAPROBLEMS IN SCREENING METAGENOMIC LIBRARIES; PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS; OTHER INDUSTRIALLY IMPORTANT ENZYMES; SUCCESS STORIES AND COMMERCIALIZED METAGENOMIC BIOCATALYSTS; CONCLUSION; REFERENCES; Chapter 7: ISOLATION OF SOIL METAGENOMIC DNA: CHALLENGES AND SOLUTIONS; ABSTRACT; INTRODUCTION; 1. ORIGIN OF METAGENOMICS; 2. SOIL -- THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES; 3. ISOLATION OF METAGENOMIC DNA; CONCLUSION; REFERENCES. Chapter 8: MICROBIAL EXPLORATION IN EXTREMECONDITIONS: METAGENOMIC ANALYSISAND FUTURE PERSPECTIVESABSTRACT; INTRODUCTION; LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS; SANGER AND NEXT GENERATION SEQUENCING; THE METAGENOMICS PIPELINE; BIOINFORMATICS AND THERMOPHILE METAGENOMICS; ANNOTATION OF METAGENOMIC SEQUENCES; DIFFERENT METAGENOMIC APPROACHES FOR MICROBIOLOGY; SEQUENCE-DRIVEN METAGENOMICS; FUNCTION-DRIVEN METAGENOMICS; EXTREME ECOLOGICAL ENVIRONMENTS; HOT WATER SPRINGS; THERMOPHILIC MICROORGANISMS IN HOT SPRINGS; THERMOPHILIC HOT SPRINGS BACTERIA; THERMOPHILIC VIRUSES. Microbial genomics. http://id.loc.gov/authorities/subjects/sh2003001301 Génomique microbienne. SCIENCE Life Sciences Biochemistry. bisacsh Microbial genomics fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh2003001301 |
| title | Metagenomics : methods, applications and perspectives / |
| title_auth | Metagenomics : methods, applications and perspectives / |
| title_exact_search | Metagenomics : methods, applications and perspectives / |
| title_full | Metagenomics : methods, applications and perspectives / Camilla Benedetti, editor. |
| title_fullStr | Metagenomics : methods, applications and perspectives / Camilla Benedetti, editor. |
| title_full_unstemmed | Metagenomics : methods, applications and perspectives / Camilla Benedetti, editor. |
| title_short | Metagenomics : |
| title_sort | metagenomics methods applications and perspectives |
| title_sub | methods, applications and perspectives / |
| topic | Microbial genomics. http://id.loc.gov/authorities/subjects/sh2003001301 Génomique microbienne. SCIENCE Life Sciences Biochemistry. bisacsh Microbial genomics fast |
| topic_facet | Microbial genomics. Génomique microbienne. SCIENCE Life Sciences Biochemistry. Microbial genomics |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=863028 |
| work_keys_str_mv | AT benedetticamilla metagenomicsmethodsapplicationsandperspectives |