Insect molecular genetics :: an introduction to principles and applications /
This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools.
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| 1. Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam :
Academic Press,
[2013]
|
| Ausgabe: | Third edition. |
| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools. |
| Beschreibung: | 1 online resource (xxvii, 808 pages) : illustrations (some color) |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 9780240821313 0240821319 |
Internformat
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| 100 | 1 | |a Hoy, Marjorie A., |e author. |0 http://id.loc.gov/authorities/names/n79094468 | |
| 245 | 1 | 0 | |a Insect molecular genetics : |b an introduction to principles and applications / |c Marjorie A. Hoy. |
| 250 | |a Third edition. | ||
| 264 | 1 | |a Amsterdam : |b Academic Press, |c [2013] | |
| 264 | 4 | |c ©2013 | |
| 300 | |a 1 online resource (xxvii, 808 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 505 | 0 | |6 880-01 |a Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. | |
| 505 | 0 | |a Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited. | |
| 520 | |a This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools. | ||
| 650 | 0 | |a Insects |x Molecular genetics. | |
| 650 | 0 | |a Arthropoda. |0 http://id.loc.gov/authorities/subjects/sh85008134 | |
| 650 | 0 | |a Insects. |0 http://id.loc.gov/authorities/subjects/sh85066670 | |
| 650 | 0 | |a Molecular structure. |0 http://id.loc.gov/authorities/subjects/sh85086594 | |
| 650 | 2 | |a Arthropods | |
| 650 | 2 | |a Chemical Phenomena | |
| 650 | 2 | |a Phenomena and Processes | |
| 650 | 2 | |a Biochemical Phenomena | |
| 650 | 2 | |a Invertebrates | |
| 650 | 2 | |a Animals | |
| 650 | 2 | |a Eukaryota | |
| 650 | 2 | |a Organisms | |
| 650 | 2 | |a Insecta | |
| 650 | 2 | |a Molecular Structure | |
| 650 | 2 | |a Genetic Phenomena | |
| 650 | 4 | |a Zoology. | |
| 650 | 4 | |a Health & Biological Sciences. | |
| 650 | 4 | |a Invertebrates & Protozoa. | |
| 650 | 6 | |a Insectes |x Génétique moléculaire. | |
| 650 | 6 | |a Arthropodes. | |
| 650 | 6 | |a Insectes. | |
| 650 | 6 | |a Structure moléculaire. | |
| 650 | 7 | |a molecular structure. |2 aat | |
| 650 | 7 | |a Insecta (class) |2 aat | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Molecular Biology. |2 bisacsh | |
| 650 | 7 | |a Molecular structure |2 fast | |
| 650 | 7 | |a Insects |2 fast | |
| 650 | 7 | |a Arthropoda |2 fast | |
| 650 | 7 | |a Insects |x Molecular genetics |2 fast | |
| 758 | |i has work: |a Insect molecular genetics (Text) |1 https://id.oclc.org/worldcat/entity/E39PCFFcPycqVF7qcfW7VhQqry |4 https://id.oclc.org/worldcat/ontology/hasWork | ||
| 776 | 0 | 8 | |i Print version: |a Hoy, Marjorie A. |t Insect molecular genetics. |b Third edition. |d Amsterdam : Academic Press, 2013 |z 9780124158740 |w (OCoLC)829055538 |
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| 880 | 0 | 0 | |6 505-01/(S |g 4.17. |t Evo-Devo and the Revolution in Developmental Studies -- |t References Cited -- |g pt. II |t MOLECULAR GENETIC TECHNIQUES -- |g ch. 5 |t Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNA -- |g 5.1. |t Overview -- |g 5.2. |t Introduction to a Basic Molecular Biology Experiment -- |g 5.2.1. |t The Pros and Cons of Kits -- |g 5.2.2. |t A Simple Cloning Experiment -- |g 5.3. |t Extracting DNA from Insects -- |g 5.3.1. |t DNA Extraction Resulting in Loss of the Specimens -- |g 5.3.2. |t DNA Extraction That Does Not Require Destroying the Specimens -- |g 5.3.3. |t Assessing the Quality of Extracted DNA -- |g 5.4. |t Precipitating Nucleic Acids -- |g 5.5. |t Shearing DNA -- |g 5.6. |t Cutting DNA with Restriction Endonucleases -- |g 5.7. |t Joining DNA Molecules -- |g 5.8. |t Growth, Maintenance, and Storage of E. coli -- |g 5.9. |t Plasmids for Cloning in E. coli -- |g 5.10. |t Transforming E. coli with Plasmids -- |g 5.11. |t Purifying Plasmid DNA from E. coli -- |g 5.12. |t Electrophoresis in Agarose or Acrylamide Gels -- |g 5.13. |t Detecting, Viewing, and Photographing Nucleic Acids in Gels -- |g 5.14. |t Identifying Specific DNA by Southern Blot Analysis -- |g 5.15. |t Labeling DNA or RNA Probes -- |g 5.16. |t Removing DNA from Agarose Gels after Electrophoresis -- |g 5.17. |t Restriction-Site Mapping -- |t General References -- |t References Cited -- |g ch. 6 |t Some Additional Tools for the Molecular Biologist -- |g 6.1. |t Overview -- |g 6.2. |t Introduction -- |g 6.3. |t The Perfect Genomic Library -- |g 6.3.1. |t Lambda (λ) Phage as a Vector -- |g 6.3.2. |t Cloning with Cosmids -- |g 6.3.3. |t Cloning in the Filamentous Phage M13 -- |g 6.3.4. |t Phagemids -- |g 6.3.5. |t BACs -- |g 6.4. |t cDNA Cloning -- |g 6.5. |t Enzymes Used in Molecular Biology Experiments -- |g 6.6. |t Isolating a Specific Gene from a Library if Whole-Genome Sequencing is Not Done -- |g 6.7. |t Labeling Probes by a Variety of Methods -- |g 6.7.1. |t Synthesis of Uniformly Labeled DNA Probes by Random Primers -- |g 6.7.2. |t Synthesis of Probes by Primer Extension -- |g 6.7.3. |t End-Labeled Probes -- |g 6.7.4. |t Single-Stranded Probes -- |g 6.7.5. |t Synthetic Probes -- |g 6.8. |t Baculovirus Vectors Express Foreign Polypeptides in Insect Cells -- |g 6.9. |t Expression Microarray Analysis -- |t General References -- |t References Cited -- |g ch. 7 |t DNA Sequencing and the Evolution of the "-Omics" -- |g 7.1. |t Overview -- |g 7.2. |t Introduction -- |g 7.3. |t The Dideoxy or Chain-Termination (Sanger) Method -- |g 7.4. |t The Maxam and Gilbert Sequencing Method -- |g 7.5. |t Shotgun Strategies for Genomes -- |g 7.6. |t Sequencing DNA by the Polymerase Chain Reaction (PCR) -- |g 7.7. |t Automated Sanger Sequencers -- |g 7.7.1. |t Decreasing Costs of Sanger Sequencing -- |g 7.8. |t Analyzing DNA Sequence Data -- |g 7.9. |t DNA-Sequence Data Banks -- |g 7.10. |t A Brief History of the Drosophila Genome Project -- |g 7.10.1. |t The Original Drosophila Genome Project -- |g 7.10.2. |t The Actual Drosophila Genome Project -- |g 7.10.3. |t Drosophila Genome Analysis -- |g 7.10.4. |t Surprises in the Drosophila Genome -- |g 7.11. |t Next-Generation Sequencing Methods and Beyond -- |g 7.11.1. |t Next-Generation (NextGen or Second-Generation) Sequencing -- |g 7.11.2. |t Third-Generation Sequencing -- |g 7.12. |t Bioinformatics -- |g 7.12.1. |t Gene Ontology -- |g 7.13. |t Genome Analyses of Other Arthropods -- |g 7.13.1. |t Interesting Findings from Completed Genomes -- |g 7.13.2. |t What Do You Need to Do to Sequence Your Favorite Insect's Genome-- |g 7.14. |t Transposable Elements (TEs) as Agents of Genome Evolution -- |g 7.15. |t Transcriptomics -- |g 7.15.1. |t Tiling Microarrays -- |g 7.16. |t Metagenomics -- |g 7.17. |t Proteomics: Another "-Omic" -- |g 7.18. |t Functional Genomics -- |g 7.19. |t Structural Genomics---Another New Horizon-- |g 7.20. |t Comparative Genomics -- |g 7.21. |t Interactomes or Reactomes -- |g 7.22. |t The Post-Genomic Era: Systems Genetics -- |t General References -- |t References Cited -- |g ch. 8 |t DNA Amplification by the Polymerase Chain Reaction: Molecular Biology Made Accessible -- |g 8.1. |t Overview -- |g 8.2. |t Introduction -- |g 8.3. |t The Basic Polymerase Chain Reaction (PCR) -- |g 8.3.1. |t The First Few Cycles are Critical -- |g 8.3.2. |t PCR Power -- |g 8.3.3. |t Standard PCR Protocols -- |g 8.3.4. |t DNA Polymerases -- |g 8.3.5. |t Other Thermostable DNA Polymerases -- |g 8.3.6. |t Primers are Primary -- |g 8.3.7. |t Storing Insects for the PCR -- |g 8.3.8. |t Preparing DNA Samples -- |g 8.3.9. |t PCR Automation -- |g 8.3.10. |t Specificity of the PCR -- |g 8.3.11. |t Detecting Primer Artifacts -- |g 8.3.12. |t How Many Cycles Does a PCR Need-- |g 8.3.13. |t Reducing the Evils of Contamination -- |g 8.4. |t Some Modifications of the PCR -- |g 8.4.1. |t AFLP for DNA Fingerprinting -- |g 8.4.2. |t Anchored PCR -- |g 8.4.3. |t Arbitrary Primers -- |g 8.4.4. |t Asymmetric PCR -- |g 8.4.5. |t Degenerate Primers -- |g 8.4.6. |t Hot-Start PCR -- |g 8.4.7. |t Inverse PCR -- |g 8.4.8. |t Long PCR or High-Fidelity PCR -- |g 8.4.9. |t Multiplex PCR -- |g 8.4.10. |t Nested PCR -- |g 8.4.11. |t PCR-RFLP -- |g 8.4.12. |t Quantitative PCR -- |g 8.4.13. |t Random Primers. |
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| contents | Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited. |
| ctrlnum | (OCoLC)839882342 |
| dewey-full | 595.7 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 595 - Arthropoda |
| dewey-raw | 595.7 |
| dewey-search | 595.7 |
| dewey-sort | 3595.7 |
| dewey-tens | 590 - Animals |
| discipline | Biologie |
| edition | Third edition. |
| format | Electronic eBook |
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Hoy.</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">Third edition.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam :</subfield><subfield code="b">Academic Press,</subfield><subfield code="c">[2013]</subfield></datafield><datafield tag="264" ind1=" " ind2="4"><subfield code="c">©2013</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 online resource (xxvii, 808 pages) :</subfield><subfield code="b">illustrations (some color)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">computer</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">online resource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="504" ind1=" " ind2=" "><subfield code="a">Includes bibliographical references and index.</subfield></datafield><datafield tag="588" ind1="0" ind2=" "><subfield code="a">Print version record.</subfield></datafield><datafield tag="505" ind1="0" ind2=" "><subfield code="6">880-01</subfield><subfield code="a">Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. </subfield></datafield><datafield tag="505" ind1="0" ind2=" "><subfield code="a">Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools.</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Insects</subfield><subfield code="x">Molecular genetics.</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Arthropoda.</subfield><subfield code="0">http://id.loc.gov/authorities/subjects/sh85008134</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Insects.</subfield><subfield code="0">http://id.loc.gov/authorities/subjects/sh85066670</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Molecular structure.</subfield><subfield code="0">http://id.loc.gov/authorities/subjects/sh85086594</subfield></datafield><datafield tag="650" ind1=" " ind2="2"><subfield code="a">Arthropods</subfield></datafield><datafield tag="650" ind1=" " ind2="2"><subfield 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code="a">SCIENCE</subfield><subfield code="x">Life Sciences</subfield><subfield code="x">Molecular Biology.</subfield><subfield code="2">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Molecular structure</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Insects</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Arthropoda</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Insects</subfield><subfield code="x">Molecular genetics</subfield><subfield code="2">fast</subfield></datafield><datafield tag="758" ind1=" " ind2=" "><subfield code="i">has work:</subfield><subfield code="a">Insect molecular genetics (Text)</subfield><subfield code="1">https://id.oclc.org/worldcat/entity/E39PCFFcPycqVF7qcfW7VhQqry</subfield><subfield code="4">https://id.oclc.org/worldcat/ontology/hasWork</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Print version:</subfield><subfield code="a">Hoy, Marjorie A.</subfield><subfield code="t">Insect molecular genetics.</subfield><subfield code="b">Third edition.</subfield><subfield code="d">Amsterdam : Academic Press, 2013</subfield><subfield code="z">9780124158740</subfield><subfield code="w">(OCoLC)829055538</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="l">FWS01</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FWS_PDA_EBA</subfield><subfield code="u">https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=486205</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="l">FWS01</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FWS_PDA_EBA</subfield><subfield code="u">https://www.sciencedirect.com/science/book/9780124158740</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="880" ind1="0" ind2="0"><subfield code="6">505-01/(S</subfield><subfield code="g">4.17.</subfield><subfield code="t">Evo-Devo and the Revolution in Developmental Studies --</subfield><subfield code="t">References Cited --</subfield><subfield code="g">pt. II</subfield><subfield code="t">MOLECULAR GENETIC TECHNIQUES --</subfield><subfield code="g">ch. 5</subfield><subfield code="t">Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNA --</subfield><subfield code="g">5.1.</subfield><subfield code="t">Overview --</subfield><subfield code="g">5.2.</subfield><subfield code="t">Introduction to a Basic Molecular Biology Experiment --</subfield><subfield code="g">5.2.1.</subfield><subfield code="t">The Pros and Cons of Kits --</subfield><subfield code="g">5.2.2.</subfield><subfield code="t">A Simple Cloning Experiment --</subfield><subfield code="g">5.3.</subfield><subfield code="t">Extracting DNA from Insects --</subfield><subfield code="g">5.3.1.</subfield><subfield code="t">DNA Extraction Resulting in Loss of the Specimens --</subfield><subfield code="g">5.3.2.</subfield><subfield code="t">DNA Extraction That Does Not Require Destroying the Specimens --</subfield><subfield code="g">5.3.3.</subfield><subfield code="t">Assessing the Quality of Extracted DNA --</subfield><subfield code="g">5.4.</subfield><subfield code="t">Precipitating Nucleic Acids --</subfield><subfield code="g">5.5.</subfield><subfield code="t">Shearing DNA --</subfield><subfield code="g">5.6.</subfield><subfield code="t">Cutting DNA with Restriction Endonucleases --</subfield><subfield code="g">5.7.</subfield><subfield code="t">Joining DNA Molecules --</subfield><subfield code="g">5.8.</subfield><subfield code="t">Growth, Maintenance, and Storage of E. coli --</subfield><subfield code="g">5.9.</subfield><subfield code="t">Plasmids for Cloning in E. coli --</subfield><subfield code="g">5.10.</subfield><subfield code="t">Transforming E. coli with Plasmids --</subfield><subfield code="g">5.11.</subfield><subfield code="t">Purifying Plasmid DNA from E. coli --</subfield><subfield code="g">5.12.</subfield><subfield code="t">Electrophoresis in Agarose or Acrylamide Gels --</subfield><subfield code="g">5.13.</subfield><subfield code="t">Detecting, Viewing, and Photographing Nucleic Acids in Gels --</subfield><subfield code="g">5.14.</subfield><subfield code="t">Identifying Specific DNA by Southern Blot Analysis --</subfield><subfield code="g">5.15.</subfield><subfield code="t">Labeling DNA or RNA Probes --</subfield><subfield code="g">5.16.</subfield><subfield code="t">Removing DNA from Agarose Gels after Electrophoresis --</subfield><subfield code="g">5.17.</subfield><subfield code="t">Restriction-Site Mapping --</subfield><subfield code="t">General References --</subfield><subfield code="t">References Cited --</subfield><subfield code="g">ch. 6</subfield><subfield code="t">Some Additional Tools for the Molecular Biologist --</subfield><subfield code="g">6.1.</subfield><subfield code="t">Overview --</subfield><subfield code="g">6.2.</subfield><subfield code="t">Introduction --</subfield><subfield code="g">6.3.</subfield><subfield code="t">The Perfect Genomic Library --</subfield><subfield code="g">6.3.1.</subfield><subfield code="t">Lambda (λ) Phage as a Vector --</subfield><subfield code="g">6.3.2.</subfield><subfield code="t">Cloning with Cosmids --</subfield><subfield code="g">6.3.3.</subfield><subfield code="t">Cloning in the Filamentous Phage M13 --</subfield><subfield code="g">6.3.4.</subfield><subfield code="t">Phagemids --</subfield><subfield code="g">6.3.5.</subfield><subfield code="t">BACs --</subfield><subfield code="g">6.4.</subfield><subfield code="t">cDNA Cloning --</subfield><subfield code="g">6.5.</subfield><subfield code="t">Enzymes Used in Molecular Biology Experiments --</subfield><subfield code="g">6.6.</subfield><subfield code="t">Isolating a Specific Gene from a Library if Whole-Genome Sequencing is Not Done --</subfield><subfield code="g">6.7.</subfield><subfield code="t">Labeling Probes by a Variety of Methods --</subfield><subfield code="g">6.7.1.</subfield><subfield code="t">Synthesis of Uniformly Labeled DNA Probes by Random Primers --</subfield><subfield code="g">6.7.2.</subfield><subfield code="t">Synthesis of Probes by Primer Extension --</subfield><subfield code="g">6.7.3.</subfield><subfield code="t">End-Labeled Probes --</subfield><subfield code="g">6.7.4.</subfield><subfield code="t">Single-Stranded Probes --</subfield><subfield code="g">6.7.5.</subfield><subfield code="t">Synthetic Probes --</subfield><subfield code="g">6.8.</subfield><subfield code="t">Baculovirus Vectors Express Foreign Polypeptides in Insect Cells --</subfield><subfield code="g">6.9.</subfield><subfield code="t">Expression Microarray Analysis --</subfield><subfield code="t">General References --</subfield><subfield code="t">References Cited --</subfield><subfield code="g">ch. 7</subfield><subfield code="t">DNA Sequencing and the Evolution of the "-Omics" --</subfield><subfield code="g">7.1.</subfield><subfield code="t">Overview --</subfield><subfield code="g">7.2.</subfield><subfield code="t">Introduction --</subfield><subfield code="g">7.3.</subfield><subfield code="t">The Dideoxy or Chain-Termination (Sanger) Method --</subfield><subfield code="g">7.4.</subfield><subfield code="t">The Maxam and Gilbert Sequencing Method --</subfield><subfield code="g">7.5.</subfield><subfield code="t">Shotgun Strategies for Genomes --</subfield><subfield code="g">7.6.</subfield><subfield code="t">Sequencing DNA by the Polymerase Chain Reaction (PCR) --</subfield><subfield code="g">7.7.</subfield><subfield code="t">Automated Sanger Sequencers --</subfield><subfield code="g">7.7.1.</subfield><subfield code="t">Decreasing Costs of Sanger Sequencing --</subfield><subfield code="g">7.8.</subfield><subfield code="t">Analyzing DNA Sequence Data --</subfield><subfield code="g">7.9.</subfield><subfield code="t">DNA-Sequence Data Banks --</subfield><subfield code="g">7.10.</subfield><subfield code="t">A Brief History of the Drosophila Genome Project --</subfield><subfield code="g">7.10.1.</subfield><subfield code="t">The Original Drosophila Genome Project --</subfield><subfield code="g">7.10.2.</subfield><subfield code="t">The Actual Drosophila Genome Project --</subfield><subfield code="g">7.10.3.</subfield><subfield code="t">Drosophila Genome Analysis --</subfield><subfield code="g">7.10.4.</subfield><subfield code="t">Surprises in the Drosophila Genome --</subfield><subfield code="g">7.11.</subfield><subfield code="t">Next-Generation Sequencing Methods and Beyond --</subfield><subfield code="g">7.11.1.</subfield><subfield code="t">Next-Generation (NextGen or Second-Generation) Sequencing --</subfield><subfield code="g">7.11.2.</subfield><subfield code="t">Third-Generation Sequencing --</subfield><subfield code="g">7.12.</subfield><subfield code="t">Bioinformatics --</subfield><subfield code="g">7.12.1.</subfield><subfield code="t">Gene Ontology --</subfield><subfield code="g">7.13.</subfield><subfield code="t">Genome Analyses of Other Arthropods --</subfield><subfield code="g">7.13.1.</subfield><subfield code="t">Interesting Findings from Completed Genomes --</subfield><subfield code="g">7.13.2.</subfield><subfield code="t">What Do You Need to Do to Sequence Your Favorite Insect's Genome--</subfield><subfield code="g">7.14.</subfield><subfield code="t">Transposable Elements (TEs) as Agents of Genome Evolution --</subfield><subfield code="g">7.15.</subfield><subfield code="t">Transcriptomics --</subfield><subfield code="g">7.15.1.</subfield><subfield code="t">Tiling Microarrays --</subfield><subfield code="g">7.16.</subfield><subfield code="t">Metagenomics --</subfield><subfield code="g">7.17.</subfield><subfield code="t">Proteomics: Another "-Omic" --</subfield><subfield code="g">7.18.</subfield><subfield code="t">Functional Genomics --</subfield><subfield code="g">7.19.</subfield><subfield code="t">Structural Genomics---Another New Horizon--</subfield><subfield code="g">7.20.</subfield><subfield code="t">Comparative Genomics --</subfield><subfield code="g">7.21.</subfield><subfield code="t">Interactomes or Reactomes --</subfield><subfield code="g">7.22.</subfield><subfield code="t">The Post-Genomic Era: Systems Genetics --</subfield><subfield code="t">General References --</subfield><subfield code="t">References Cited --</subfield><subfield code="g">ch. 8</subfield><subfield code="t">DNA Amplification by the Polymerase Chain Reaction: Molecular Biology Made Accessible --</subfield><subfield code="g">8.1.</subfield><subfield code="t">Overview --</subfield><subfield code="g">8.2.</subfield><subfield code="t">Introduction --</subfield><subfield code="g">8.3.</subfield><subfield code="t">The Basic Polymerase Chain Reaction (PCR) --</subfield><subfield code="g">8.3.1.</subfield><subfield code="t">The First Few Cycles are Critical --</subfield><subfield code="g">8.3.2.</subfield><subfield code="t">PCR Power --</subfield><subfield code="g">8.3.3.</subfield><subfield code="t">Standard PCR Protocols --</subfield><subfield code="g">8.3.4.</subfield><subfield code="t">DNA 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| id | ZDB-4-EBA-ocn839882342 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:25:18Z |
| institution | BVB |
| isbn | 9780240821313 0240821319 |
| language | English |
| oclc_num | 839882342 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xxvii, 808 pages) : illustrations (some color) |
| psigel | ZDB-4-EBA |
| publishDate | 2013 |
| publishDateSearch | 2013 |
| publishDateSort | 2013 |
| publisher | Academic Press, |
| record_format | marc |
| spelling | Hoy, Marjorie A., author. http://id.loc.gov/authorities/names/n79094468 Insect molecular genetics : an introduction to principles and applications / Marjorie A. Hoy. Third edition. Amsterdam : Academic Press, [2013] ©2013 1 online resource (xxvii, 808 pages) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier Includes bibliographical references and index. Print version record. 880-01 Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited. This book summarizes and synthesizes two rather disparate disciplines-entomology and molecular genetics. It provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools. Insects Molecular genetics. Arthropoda. http://id.loc.gov/authorities/subjects/sh85008134 Insects. http://id.loc.gov/authorities/subjects/sh85066670 Molecular structure. http://id.loc.gov/authorities/subjects/sh85086594 Arthropods Chemical Phenomena Phenomena and Processes Biochemical Phenomena Invertebrates Animals Eukaryota Organisms Insecta Molecular Structure Genetic Phenomena Zoology. Health & Biological Sciences. Invertebrates & Protozoa. Insectes Génétique moléculaire. Arthropodes. Insectes. Structure moléculaire. molecular structure. aat Insecta (class) aat SCIENCE Life Sciences Molecular Biology. bisacsh Molecular structure fast Insects fast Arthropoda fast Insects Molecular genetics fast has work: Insect molecular genetics (Text) https://id.oclc.org/worldcat/entity/E39PCFFcPycqVF7qcfW7VhQqry https://id.oclc.org/worldcat/ontology/hasWork Print version: Hoy, Marjorie A. Insect molecular genetics. Third edition. Amsterdam : Academic Press, 2013 9780124158740 (OCoLC)829055538 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=486205 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780124158740 Volltext 505-01/(S 4.17. Evo-Devo and the Revolution in Developmental Studies -- References Cited -- pt. II MOLECULAR GENETIC TECHNIQUES -- ch. 5 Some Basic Tools: How to Cut, Paste, Copy, Measure, Visualize, and Clone DNA -- 5.1. Overview -- 5.2. Introduction to a Basic Molecular Biology Experiment -- 5.2.1. The Pros and Cons of Kits -- 5.2.2. A Simple Cloning Experiment -- 5.3. Extracting DNA from Insects -- 5.3.1. DNA Extraction Resulting in Loss of the Specimens -- 5.3.2. DNA Extraction That Does Not Require Destroying the Specimens -- 5.3.3. Assessing the Quality of Extracted DNA -- 5.4. Precipitating Nucleic Acids -- 5.5. Shearing DNA -- 5.6. Cutting DNA with Restriction Endonucleases -- 5.7. Joining DNA Molecules -- 5.8. Growth, Maintenance, and Storage of E. coli -- 5.9. Plasmids for Cloning in E. coli -- 5.10. Transforming E. coli with Plasmids -- 5.11. Purifying Plasmid DNA from E. coli -- 5.12. Electrophoresis in Agarose or Acrylamide Gels -- 5.13. Detecting, Viewing, and Photographing Nucleic Acids in Gels -- 5.14. Identifying Specific DNA by Southern Blot Analysis -- 5.15. Labeling DNA or RNA Probes -- 5.16. Removing DNA from Agarose Gels after Electrophoresis -- 5.17. Restriction-Site Mapping -- General References -- References Cited -- ch. 6 Some Additional Tools for the Molecular Biologist -- 6.1. Overview -- 6.2. Introduction -- 6.3. The Perfect Genomic Library -- 6.3.1. Lambda (λ) Phage as a Vector -- 6.3.2. Cloning with Cosmids -- 6.3.3. Cloning in the Filamentous Phage M13 -- 6.3.4. Phagemids -- 6.3.5. BACs -- 6.4. cDNA Cloning -- 6.5. Enzymes Used in Molecular Biology Experiments -- 6.6. Isolating a Specific Gene from a Library if Whole-Genome Sequencing is Not Done -- 6.7. Labeling Probes by a Variety of Methods -- 6.7.1. Synthesis of Uniformly Labeled DNA Probes by Random Primers -- 6.7.2. Synthesis of Probes by Primer Extension -- 6.7.3. End-Labeled Probes -- 6.7.4. Single-Stranded Probes -- 6.7.5. Synthetic Probes -- 6.8. Baculovirus Vectors Express Foreign Polypeptides in Insect Cells -- 6.9. Expression Microarray Analysis -- General References -- References Cited -- ch. 7 DNA Sequencing and the Evolution of the "-Omics" -- 7.1. Overview -- 7.2. Introduction -- 7.3. The Dideoxy or Chain-Termination (Sanger) Method -- 7.4. The Maxam and Gilbert Sequencing Method -- 7.5. Shotgun Strategies for Genomes -- 7.6. Sequencing DNA by the Polymerase Chain Reaction (PCR) -- 7.7. Automated Sanger Sequencers -- 7.7.1. Decreasing Costs of Sanger Sequencing -- 7.8. Analyzing DNA Sequence Data -- 7.9. DNA-Sequence Data Banks -- 7.10. A Brief History of the Drosophila Genome Project -- 7.10.1. The Original Drosophila Genome Project -- 7.10.2. The Actual Drosophila Genome Project -- 7.10.3. Drosophila Genome Analysis -- 7.10.4. Surprises in the Drosophila Genome -- 7.11. Next-Generation Sequencing Methods and Beyond -- 7.11.1. Next-Generation (NextGen or Second-Generation) Sequencing -- 7.11.2. Third-Generation Sequencing -- 7.12. Bioinformatics -- 7.12.1. Gene Ontology -- 7.13. Genome Analyses of Other Arthropods -- 7.13.1. Interesting Findings from Completed Genomes -- 7.13.2. What Do You Need to Do to Sequence Your Favorite Insect's Genome-- 7.14. Transposable Elements (TEs) as Agents of Genome Evolution -- 7.15. Transcriptomics -- 7.15.1. Tiling Microarrays -- 7.16. Metagenomics -- 7.17. Proteomics: Another "-Omic" -- 7.18. Functional Genomics -- 7.19. Structural Genomics---Another New Horizon-- 7.20. Comparative Genomics -- 7.21. Interactomes or Reactomes -- 7.22. The Post-Genomic Era: Systems Genetics -- General References -- References Cited -- ch. 8 DNA Amplification by the Polymerase Chain Reaction: Molecular Biology Made Accessible -- 8.1. Overview -- 8.2. Introduction -- 8.3. The Basic Polymerase Chain Reaction (PCR) -- 8.3.1. The First Few Cycles are Critical -- 8.3.2. PCR Power -- 8.3.3. Standard PCR Protocols -- 8.3.4. DNA Polymerases -- 8.3.5. Other Thermostable DNA Polymerases -- 8.3.6. Primers are Primary -- 8.3.7. Storing Insects for the PCR -- 8.3.8. Preparing DNA Samples -- 8.3.9. PCR Automation -- 8.3.10. Specificity of the PCR -- 8.3.11. Detecting Primer Artifacts -- 8.3.12. How Many Cycles Does a PCR Need-- 8.3.13. Reducing the Evils of Contamination -- 8.4. Some Modifications of the PCR -- 8.4.1. AFLP for DNA Fingerprinting -- 8.4.2. Anchored PCR -- 8.4.3. Arbitrary Primers -- 8.4.4. Asymmetric PCR -- 8.4.5. Degenerate Primers -- 8.4.6. Hot-Start PCR -- 8.4.7. Inverse PCR -- 8.4.8. Long PCR or High-Fidelity PCR -- 8.4.9. Multiplex PCR -- 8.4.10. Nested PCR -- 8.4.11. PCR-RFLP -- 8.4.12. Quantitative PCR -- 8.4.13. Random Primers. |
| spellingShingle | Hoy, Marjorie A. Insect molecular genetics : an introduction to principles and applications / Note continued: 9.3.P Elements and Hybrid Dysgenesis -- 9.4.P-Element Structure Varies -- 9.5. Transposition Method of P Elements -- 9.6. Origin of P Elements in D. melanogaster -- 9.7.P Vectors and Germ-Line Transformation -- 9.7.1. Protocols -- 9.7.2. Characterizing Transformants -- 9.8. Using P-Element Vectors -- 9.8.1. Transposon Tagging -- 9.8.2. Expressing Exogenous Genes -- 9.8.3. Evaluating Position Effects -- 9.8.4. Targeted Gene Transfer -- 9.9. Transformation of Other Insects with P Vectors -- 9.10. Evolution of Resistance to P Elements -- 9.11. Using P to Drive Genes into Populations -- 9.12. Relationship of P to Other Transposable Elements (TEs) -- 9.13. Other TEs Can Transform D. melanogaster -- 9.14. Improved Transformation Tools for Drosophila -- 9.15. TE Vectors to Transform Insects other than Drosophila -- 9.15.1.piggyBac -- 9.15.2. Hermes and Herves -- 9.15.3. Minos -- 9.15.4.mariner -- 9.15.5.hobo -- 9.16. Cross Mobilization of TE Vectors -- 9.17. Conversion of Inactive TE Vectors to Activity -- 9.18. Suppression of Transgene Expression -- 9.19. Other Transformation Methods -- 9.19.1. JcDNV Gene Vectors for Somatic Transformations v -- 9.19.2. RNAi for Drosophila -- 9.19.3. Zinc-Finger Nucleases (ZFNs) -- 9.19.4. Transcription Activator-Like Effector Nucleases (TALENs) -- 9.19.5. Meganucleases (or Homing Endonucleases) -- 9.19.6. Cell-Penetrating Peptides -- 9.19.7. Nanotechnology Approaches -- 9.20. Conclusions -- General References -- References Cited -- pt. III APPLICATIONS IN ENTOMOLOGY -- ch. 10 Sex Determination in Insects -- 10.1. Overview -- 10.2. Introduction -- 10.3. Costs and Benefits of Sexual Reproduction -- 10.3.1. Sexual Reproduction Has Costs -- 10.3.2. Advantages of Sex Must Be Large -- 10.3.3. Origin of Sex -- 10.4. Sex Determination Involves Soma and Germ-Line Tissues -- 10.5. Sex Determination in Drosophila melanogaster -- 10.5.1. Dosage Compensation of X Chromosomes -- 10.5.2. Somatic-Sex Determination -- 10.5.3. Germ-Line Determination -- 10.6. Are Sex-Determination Mechanisms Diverse? -- 10.6.1. Intraspecific Variability -- 10.6.2. Environmental Effects -- 10.6.3. Postzygotic Sex Determination -- 10.7.A Single Model? -- 10.8. Meiotic Drive Can Distort Sex Ratios -- 10.8.1. Segregation Distorter (SD) -- 10.8.2. Distorter in Mosquitoes -- 10.8.3. Female-Biased Sex Ratios in Stalk-Eyed Flies -- 10.8.4. Meiotic Drive as a Pest-Management Tool? -- 10.9. Hybrid Sterility -- 10.10. Medea in Tribolium -- 10.11. Cytoplasmic Agents Distort Normal Sex Ratios -- 10.11.1. Spiroplasma Strains -- 10.11.2.L-Form Bacteria -- 10.11.3. Rickettsia -- 10.11.4. Wolbachia -- 10.11.5. Cardinium -- 10.12. Paternal Sex-Ratio Chromosomes and Cytoplasmic Incompatibility in Nasonia -- 10.13. Male Killing in the Coccinellidae -- 10.14. Sex and the Sorted Insects -- 10.14.1. Genetic Control -- 10.14.2. Genetic Improvement of Parasitoids -- 10.15. Conclusion -- References Cited -- ch. 11 Molecular Genetics of Insect Behavior -- 11.1. Overview -- 11.2. Introduction -- 11.3. The Insect Nervous System -- 11.4. Traditional Genetic Analyses of Behavior -- 11.4.1. Crossing Experiments -- 11.4.2. Selection Experiments -- 11.4.3. Some Polygenically Determined Behaviors -- 11.5. Molecular-Genetic Analyses of Insect Behavior -- 11.5.1. The Photoperiodic Clock -- 11.5.2. Learning in Drosophila -- 11.5.3. Functional Genomics of Odor Behavior in Drosophila -- 11.5.4. Behavior of Apis mellifera -- 11.5.5. Pheromones in Insects -- 11.5.6. Neurobiochemistry of Drosophila -- 11.5.7. Divergent Functions of Est-6 and Est-5 in Two Drosophila Species: A Cautionary Tale of Homologs -- 11.5.8. Courtship Behavior in Drosophila -- 11.5.9. Speciation Genes in Drosophila and Other Insects -- 11.5.10. Personality in Insects: Tribolium confusum, Apis mellifera, Acyrthosiphon pisum, and Pyrrhocoris apterus -- 11.6. Symbionts and Insect Behavior -- 11.7. Human Neurodegenerative Diseases and Addictions in Drosophila -- 11.8. High-Throughput Ethomics -- 11.9. Systems Genetics of Complex Traits in Drosophila -- 11.10. Social Behavior in Bees and Ants -- 11.11. Conclusions -- References Cited -- ch. 12 Molecular Systematics and the Evolution of Arthropods -- 12.1. Overview -- 12.2. Introduction -- 12.3. Controversies in Molecular Systematics and Evolution -- 12.3.1. Molecular versus Morphological Traits -- 12.3.2. The Molecular Clock -- 12.3.3. The Neutral (or Nearly Neutral) Theory of Evolution -- 12.3.4. Homology and Similarity -- 12.4. Molecular Methods for Molecular Systematics and Evolution -- 12.4.1. Protein Electrophoresis -- 12.4.2. Molecular Cytology -- 12.4.3. Restriction Fragment Length Polymorphism (RFLP) Analysis -- 12.4.4. DNA and Genome Sequencing -- 12.4.5. Fragment Analyses of Genomic DNA -- 12.5. Targets of DNA Analysis -- 12.5.1. Mitochondria -- 12.5.2. Ribosomal RNA -- 12.5.3. Satellite DNA -- 12.5.4. Introns -- 12.5.5. Nuclear Protein-Coding Genes -- 12.5.6. Rare Genomic Changes -- 12.5.7. MicroRNAs -- 12.6. Steps in Phylogenetic Analysis of DNA Sequence Data -- 12.6.1. Gene Trees or Species Trees -- 12.6.2. Rooted or Unrooted Trees -- 12.6.3. Tree Types -- 12.6.4. Project Goals and Appropriate DNA Sequences -- 12.6.5. Sequence Comparisons with BLAST -- 12.6.6. Aligning Sequences -- 12.6.7. Constructing Phylogenies -- 12.6.8. Artifacts -- 12.6.9. Software Packages -- 12.7. The Universal Tree of Life -- 12.7.1. Two Domains -- 12.7.2. Three Domains -- 12.7.3. Origin of Eukaryota -- 12.8. The Fossil Record of Arthropods -- 12.9. Molecular Analyses of Arthropod Phylogeny -- 12.9.1. Evolution of the Ecdysozoa -- 12.9.2. Relationships among the Arthropoda -- 12.9.3. The Phylogeny of the Holometabola -- 12.9.4. Congruence Between Morphology- and Molecular-Based Trees -- 12.9.5. Genomes and Arthropod Phylogenies -- 12.10. Molecular Evolution and Speciation -- 12.10.1. Species Concepts -- 12.10.2. How Many Genes are Involved in Speciation? -- 12.10.3. Detecting Cryptic Species -- 12.11. Some Conclusions -- Relevant Journals -- References Cited -- ch. 13 Insect Population Ecology and Molecular Genetics -- 13.1. Overview -- 13.2. Introduction -- 13.3. What is Molecular Ecology? -- 13.4. Collecting Arthropods in the Field for Analysis -- 13.5. Molecular Ecological Methods -- 13.5.1. Allele-Specific PCR -- 13.5.2. Allozymes (Protein Electrophoresis) -- 13.5.3. Amplified Fragment Length Polymorphisms (AFLP-PCR) -- 13.5.4. Double-Strand Conformation Polymorphism (DSCP) -- 13.5.5. Heteroduplex Analysis (HDA) -- 13.5.6. Microarrays -- 13.5.7. Microsatellites -- 13.5.8. RFLP Analysis -- 13.5.9. PCR-RFLP -- 13.5.10. RAPD-PCR -- 13.5.11. Sequencing -- 13.5.12. Single Nucleotide Polymorphism (SNP) Markers -- 13.6. Analysis of Molecular Data -- 13.6.1. Allozymes -- 13.6.2. Microsatellites -- 13.6.3. RAPD-PCR -- 13.6.4. RFLPs -- 13.6.5. Sequencing -- 13.7. Case Studies in Molecular Ecology and Population Biology -- 13.7.1. Genetic Variability in the Fall Army worm: Incipient Species or Multiple Species? -- 13.7.2. Analyses of Natural Enemies -- 13.7.3. Population Isolation and Introgression in Periodical Cicadas -- 13.7.4. Eradicating Medflies in California? -- 13.7.5. Plant Defenses to Insect Herbivory -- 13.7.6. Origins of Insect Populations -- 13.8. Applied Pest Management -- 13.8.1. Monitoring Biotypes, Species, and Cryptic Species -- 13.8.2. Monitoring Vectors of Disease -- 13.8.3. Pesticide Resistances and Pest Management -- 13.8.4. Monitoring Pest-Population Biology -- 13.8.5. The "So What?" Test -- Relevant Journals -- References Cited -- ch. 14 Genetic Modification of Pest and Beneficial Insects for Pest-Management Programs -- 14.1. Overview -- 14.2. Introduction -- 14.3. Why Genetically Modify Insects? -- 14.3.1. Beneficial Insects -- 14.3.2. Pest Insects -- 14.4. Why Use Molecular-Genetic Methods? -- 14.5. What Genetic Modification Methods are Available? -- 14.5.1. Transposable-Element (TE) Vectors and Transgenesis -- 14.5.2. Paratransgenesis (Genetic Modification of Symbionts) -- 14.5.3. Viral Vectors -- 14.5.4. Transfer of Wolbachia from Another Arthropod -- 14.5.5. Site-Specific Modifications -- 14.5.6. No Vectors -- 14.5.7. RNAi to Control Pests -- 14.6. Methods to Deliver Exogenous Nucleic Acids into Arthropod Tissues -- 14.7. What Genes are Available? -- 14.8. Why are Regulatory Signals Important? -- 14.9. How are Modified Arthropods Identified? -- 14.10. How to Deploy Genetically Modified Pest and Beneficial Arthropods -- 14.11. Potential Risks Associated with Releases of Genetically Modified Arthropods -- 14.11.1. Could Gene Silencing Reduce Program Effectiveness? -- 14.11.2. Relative Risks -- 14.11.3. General Risk Issues -- 14.11.4. Horizontal Transfer (HT) -- 14.12. Permanent Releases of Genetically Modified Arthropods into the Environment -- 14.12.1. Models to Predict? -- 14.13. Regulatory Issues: Releases of Genetically Modified Arthropods -- 14.14. Conclusions -- References Cited. Insects Molecular genetics. Arthropoda. http://id.loc.gov/authorities/subjects/sh85008134 Insects. http://id.loc.gov/authorities/subjects/sh85066670 Molecular structure. http://id.loc.gov/authorities/subjects/sh85086594 Arthropods Chemical Phenomena Phenomena and Processes Biochemical Phenomena Invertebrates Animals Eukaryota Organisms Insecta Molecular Structure Genetic Phenomena Zoology. Health & Biological Sciences. Invertebrates & Protozoa. Insectes Génétique moléculaire. Arthropodes. Insectes. Structure moléculaire. molecular structure. aat Insecta (class) aat SCIENCE Life Sciences Molecular Biology. bisacsh Molecular structure fast Insects fast Arthropoda fast Insects Molecular genetics fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh85008134 http://id.loc.gov/authorities/subjects/sh85066670 http://id.loc.gov/authorities/subjects/sh85086594 |
| title | Insect molecular genetics : an introduction to principles and applications / |
| title_auth | Insect molecular genetics : an introduction to principles and applications / |
| title_exact_search | Insect molecular genetics : an introduction to principles and applications / |
| title_full | Insect molecular genetics : an introduction to principles and applications / Marjorie A. Hoy. |
| title_fullStr | Insect molecular genetics : an introduction to principles and applications / Marjorie A. Hoy. |
| title_full_unstemmed | Insect molecular genetics : an introduction to principles and applications / Marjorie A. Hoy. |
| title_short | Insect molecular genetics : |
| title_sort | insect molecular genetics an introduction to principles and applications |
| title_sub | an introduction to principles and applications / |
| topic | Insects Molecular genetics. Arthropoda. http://id.loc.gov/authorities/subjects/sh85008134 Insects. http://id.loc.gov/authorities/subjects/sh85066670 Molecular structure. http://id.loc.gov/authorities/subjects/sh85086594 Arthropods Chemical Phenomena Phenomena and Processes Biochemical Phenomena Invertebrates Animals Eukaryota Organisms Insecta Molecular Structure Genetic Phenomena Zoology. Health & Biological Sciences. Invertebrates & Protozoa. Insectes Génétique moléculaire. Arthropodes. Insectes. Structure moléculaire. molecular structure. aat Insecta (class) aat SCIENCE Life Sciences Molecular Biology. bisacsh Molecular structure fast Insects fast Arthropoda fast Insects Molecular genetics fast |
| topic_facet | Insects Molecular genetics. Arthropoda. Insects. Molecular structure. Arthropods Chemical Phenomena Phenomena and Processes Biochemical Phenomena Invertebrates Animals Eukaryota Organisms Insecta Molecular Structure Genetic Phenomena Zoology. Health & Biological Sciences. Invertebrates & Protozoa. Insectes Génétique moléculaire. Arthropodes. Insectes. Structure moléculaire. molecular structure. Insecta (class) SCIENCE Life Sciences Molecular Biology. Molecular structure Insects Arthropoda Insects Molecular genetics |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=486205 https://www.sciencedirect.com/science/book/9780124158740 |
| work_keys_str_mv | AT hoymarjoriea insectmoleculargeneticsanintroductiontoprinciplesandapplications |