Molecular biology techniques :: an intensive laboratory course /
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
San Diego ; London :
Academic Press,
1999.
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| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter. |
| Beschreibung: | 1 online resource (x, 234 pages) |
| ISBN: | 9780080536828 0080536824 9780125839907 0125839901 |
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| 100 | 1 | |a Ream, Walt. |0 http://id.loc.gov/authorities/names/n97017853 | |
| 245 | 1 | 0 | |a Molecular biology techniques : |b an intensive laboratory course / |c Walt Ream and Katharine G. Field. |
| 260 | |a San Diego ; |a London : |b Academic Press, |c 1999. | ||
| 300 | |a 1 online resource (x, 234 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
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| 588 | 0 | |a Print version record. | |
| 505 | 0 | |a Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index. | |
| 520 | |a This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter. | ||
| 650 | 0 | |a Molecular biology |x Technique. |0 http://id.loc.gov/authorities/subjects/sh85086579 | |
| 650 | 0 | |a Molecular biology |v Laboratory manuals. | |
| 650 | 2 | |a Molecular Biology |0 https://id.nlm.nih.gov/mesh/D008967 | |
| 650 | 6 | |a Biologie moléculaire |v Manuels de laboratoire. | |
| 650 | 6 | |a Biologie moléculaire |x Technique. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Molecular Biology. |2 bisacsh | |
| 650 | 7 | |a Molecular biology |2 fast | |
| 650 | 7 | |a Molecular biology |x Technique |2 fast | |
| 653 | 0 | 0 | |a moleculaire biologie |
| 653 | 0 | 0 | |a molecular biology |
| 653 | 0 | 0 | |a laboratoriummethoden |
| 653 | 0 | 0 | |a laboratory methods |
| 653 | 0 | 0 | |a biologische technieken |
| 653 | 0 | 0 | |a biological techniques |
| 653 | 0 | 0 | |a dna sequencing |
| 653 | 0 | 0 | |a southern blotting |
| 653 | 0 | 0 | |a northern blotting |
| 653 | 0 | 0 | |a western blotting |
| 653 | 0 | 0 | |a mutagenese |
| 653 | 0 | 0 | |a mutagenesis |
| 653 | 0 | 0 | |a genexpressie |
| 653 | 0 | 0 | |a gene expression |
| 653 | 0 | 0 | |a polymerase chain reaction |
| 653 | 0 | 0 | |a dna cloning |
| 653 | 1 | 0 | |a Molecular Biology (General) |
| 653 | 1 | 0 | |a Moleculaire biologie (algemeen) |
| 655 | 2 | |a Laboratory Manual |0 https://id.nlm.nih.gov/mesh/D020484 | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
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| contents | Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index. |
| ctrlnum | (OCoLC)173520343 |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8 |
| dewey-search | 572.8 |
| dewey-sort | 3572.8 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Electronic eBook |
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| genre | Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Manuels de laboratoire. rvmgf |
| genre_facet | Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| id | ZDB-4-EBA-ocn173520343 |
| illustrated | Not Illustrated |
| indexdate | 2024-11-27T13:16:09Z |
| institution | BVB |
| isbn | 9780080536828 0080536824 9780125839907 0125839901 |
| language | English |
| oclc_num | 173520343 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (x, 234 pages) |
| psigel | ZDB-4-EBA |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Academic Press, |
| record_format | marc |
| spelling | Ream, Walt. http://id.loc.gov/authorities/names/n97017853 Molecular biology techniques : an intensive laboratory course / Walt Ream and Katharine G. Field. San Diego ; London : Academic Press, 1999. 1 online resource (x, 234 pages) text txt rdacontent computer c rdamedia online resource cr rdacarrier Print version record. Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index. This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter. Molecular biology Technique. http://id.loc.gov/authorities/subjects/sh85086579 Molecular biology Laboratory manuals. Molecular Biology https://id.nlm.nih.gov/mesh/D008967 Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique. SCIENCE Life Sciences Molecular Biology. bisacsh Molecular biology fast Molecular biology Technique fast moleculaire biologie molecular biology laboratoriummethoden laboratory methods biologische technieken biological techniques dna sequencing southern blotting northern blotting western blotting mutagenese mutagenesis genexpressie gene expression polymerase chain reaction dna cloning Molecular Biology (General) Moleculaire biologie (algemeen) Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Manuels de laboratoire. rvmgf Field, Katharine G. http://id.loc.gov/authorities/names/n81069180 has work: Molecular biology techniques (Text) https://id.oclc.org/worldcat/entity/E39PCH4YwkjWYvvpR4JDJmCM6C https://id.oclc.org/worldcat/ontology/hasWork Print version: Ream, Walt. Molecular biology techniques. San Diego ; London : Academic Press, 1999 0125839901 9780125839907 (OCoLC)60152721 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=203136 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780125839907 Volltext |
| spellingShingle | Ream, Walt Molecular biology techniques : an intensive laboratory course / Preface. -- Course Synopsis: -- Introduction. -- Safety Precautions. -- Daily Schedule. -- Acknowledgements. -- Exercises: I. DNA Preparation, Polymerase Chain Reaction, and Molecular Cloning: -- Cesium chloride-ethidium bromide density gradient centrifugation. -- PCR to synthesize virD2 flanked with restriction sites. -- Restriction digests of plasmid pGEX2 and PCR products. -- Purification of DNA fragments from agarose. -- Ligation of PCR product to pGEX2 vector. -- Transformation of E. coli with the ligated plasmid. -- Small-scale preparation of plasmid DNA by the alkaline lysis method. -- Restriction analysis. -- Study questions. -- Protein Expression, Purification, and Analysis: -- Expression and purification of a fusion protein. -- SDS-polyacrylamide gel electrophoresis. -- Silver stain detection of proteins. -- Study questions. -- Oligonucleotide-Directed Mutagenesis: -- Restriction digests of virD2 (in pCS64) and pUC119. -- Purification of DNA fragments from agarose. -- Ligatation of restriction fragment and vector. -- Transformation of E. coli with the ligated plasmid and recovery clones. -- Small-scale preparation of plasmid DNA from broth cultures. -- Restriction digest of DNA: examination to confirm insert. -- Preparation of single-stranded DNA template. -- Phosphorylation of oligonucleotide. -- Annealing mutant oligonuleotide to template. In vitro DNA synthesis by primer extension. -- Transform synthesis reaction into E. coli DH5a. Small-scale preparation of plasmid DNA. -- Confirmation of mutants by restriction analysis. -- Study questions. -- DNA Sequencing: -- Polyacrylamide sequencing gel electrophoresis. -- Didexy sequencing. -- Automated Sequencing. -- Introduction to databases and gene sequence analysis. -- Study questions. -- Southern Blot Detection of DNA: -- Preparation of genomic DNA from Agrobacterium tumefaciens. -- Restriction digestion of genomic DNA. -- Agarose gel electrophoresis of restriction fragments. -- Southern blot: denaturation and blotting of DNA gel. -- Preparation of probe nick translation. -- Hybridization and washing of Southern blots. -- Study questions. -- Northern Blot Detection of mRNA: -- Preparation of RNA from tobacco leaves. -- Agarose-formaldehyde gel electrophoresis. -- Northern blot: denaturation and blotting of RNA. -- Probe preparation. Hybridization and washing of Northern blots. -- Study questions. -- Protein Interaction Analysis in Yeast: -- Yeast transformation. -- Filter b-galactosidase assay. -- Index. Molecular biology Technique. http://id.loc.gov/authorities/subjects/sh85086579 Molecular biology Laboratory manuals. Molecular Biology https://id.nlm.nih.gov/mesh/D008967 Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique. SCIENCE Life Sciences Molecular Biology. bisacsh Molecular biology fast Molecular biology Technique fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh85086579 https://id.nlm.nih.gov/mesh/D008967 https://id.nlm.nih.gov/mesh/D020484 http://id.loc.gov/authorities/genreForms/gf2014026120 |
| title | Molecular biology techniques : an intensive laboratory course / |
| title_auth | Molecular biology techniques : an intensive laboratory course / |
| title_exact_search | Molecular biology techniques : an intensive laboratory course / |
| title_full | Molecular biology techniques : an intensive laboratory course / Walt Ream and Katharine G. Field. |
| title_fullStr | Molecular biology techniques : an intensive laboratory course / Walt Ream and Katharine G. Field. |
| title_full_unstemmed | Molecular biology techniques : an intensive laboratory course / Walt Ream and Katharine G. Field. |
| title_short | Molecular biology techniques : |
| title_sort | molecular biology techniques an intensive laboratory course |
| title_sub | an intensive laboratory course / |
| topic | Molecular biology Technique. http://id.loc.gov/authorities/subjects/sh85086579 Molecular biology Laboratory manuals. Molecular Biology https://id.nlm.nih.gov/mesh/D008967 Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique. SCIENCE Life Sciences Molecular Biology. bisacsh Molecular biology fast Molecular biology Technique fast |
| topic_facet | Molecular biology Technique. Molecular biology Laboratory manuals. Molecular Biology Biologie moléculaire Manuels de laboratoire. Biologie moléculaire Technique. SCIENCE Life Sciences Molecular Biology. Molecular biology Molecular biology Technique Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=203136 https://www.sciencedirect.com/science/book/9780125839907 |
| work_keys_str_mv | AT reamwalt molecularbiologytechniquesanintensivelaboratorycourse AT fieldkatharineg molecularbiologytechniquesanintensivelaboratorycourse |