Molecular imaging :: FRET microscopy and spectroscopy /
The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and t...
Gespeichert in:
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| Weitere Verfasser: | , |
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Oxford ; New York :
Published for the American Physiological Society by Oxford University Press,
2005.
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| Schriftenreihe: | Methods in physiology series.
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| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques. |
| Beschreibung: | 1 online resource (xv, 312 pages) : illustrations (some color) |
| Format: | Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 9780195177206 0195177207 9780080536873 0080536875 1281033820 9781281033826 9786611033828 6611033823 |
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| 245 | 0 | 0 | |a Molecular imaging : |b FRET microscopy and spectroscopy / |c edited by Ammasi Periasamy, Richard N. Day. |
| 260 | |a Oxford ; |a New York : |b Published for the American Physiological Society by Oxford University Press, |c 2005. | ||
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| 520 | |a The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques. | ||
| 505 | 0 | |a Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy -- Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization. | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 506 | |3 Use copy |f Restrictions unspecified |2 star |5 MiAaHDL | ||
| 533 | |a Electronic reproduction. |b [Place of publication not identified] : |c HathiTrust Digital Library, |d 2010. |5 MiAaHDL | ||
| 538 | |a Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. |u http://purl.oclc.org/DLF/benchrepro0212 |5 MiAaHDL | ||
| 583 | 1 | |a digitized |c 2010 |h HathiTrust Digital Library |l committed to preserve |2 pda |5 MiAaHDL | |
| 546 | |a English. | ||
| 650 | 0 | |a Fluorescence microscopy. |0 http://id.loc.gov/authorities/subjects/sh85049410 | |
| 650 | 0 | |a Fluorescence spectroscopy. |0 http://id.loc.gov/authorities/subjects/sh85049411 | |
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| 650 | 2 | |a Microscopy, Fluorescence |x methods | |
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| 650 | 2 | |a Spectrometry, Fluorescence |x methods | |
| 650 | 2 | |a Fluorescence Resonance Energy Transfer | |
| 650 | 6 | |a Microscopie de fluorescence. | |
| 650 | 6 | |a Spectroscopie de fluorescence. | |
| 650 | 7 | |a fluorescence microscopy. |2 aat | |
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| contents | Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy -- Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization. |
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| genre | dissertations. aat Academic theses fast Academic theses. lcgft http://id.loc.gov/authorities/genreForms/gf2014026039 Thèses et écrits académiques. rvmgf Internet Resources. |
| genre_facet | dissertations. Academic theses Academic theses. Thèses et écrits académiques. Internet Resources. |
| id | ZDB-4-EBA-ocn162131550 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:16:06Z |
| institution | BVB |
| institution_GND | http://id.loc.gov/authorities/names/n50060285 |
| isbn | 9780195177206 0195177207 9780080536873 0080536875 1281033820 9781281033826 9786611033828 6611033823 |
| language | English |
| oclc_num | 162131550 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xv, 312 pages) : illustrations (some color) |
| psigel | ZDB-4-EBA |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| publisher | Published for the American Physiological Society by Oxford University Press, |
| record_format | marc |
| series | Methods in physiology series. |
| series2 | The American Physiological Society methods in physiology series |
| spelling | Molecular imaging : FRET microscopy and spectroscopy / edited by Ammasi Periasamy, Richard N. Day. Oxford ; New York : Published for the American Physiological Society by Oxford University Press, 2005. 1 online resource (xv, 312 pages) : illustrations (some color) text txt rdacontent computer c rdamedia online resource cr rdacarrier text file The American Physiological Society methods in physiology series The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques. Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy -- Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization. Includes bibliographical references and index. Print version record. Use copy Restrictions unspecified star MiAaHDL Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL English. Fluorescence microscopy. http://id.loc.gov/authorities/subjects/sh85049410 Fluorescence spectroscopy. http://id.loc.gov/authorities/subjects/sh85049411 Microscopy, Fluorescence Microscopy, Fluorescence methods Spectrometry, Fluorescence Spectrometry, Fluorescence methods Fluorescence Resonance Energy Transfer Microscopie de fluorescence. Spectroscopie de fluorescence. fluorescence microscopy. aat NATURE Reference. bisacsh SCIENCE Life Sciences General. bisacsh SCIENCE Life Sciences Biology. bisacsh Fluorescence microscopy fast Fluorescence spectroscopy fast microscopie microscopy spectroscopie spectroscopy biologische technieken biological techniques celbiologie cellular biology beeldvormende spectroscopie imaging spectroscopy Cellular Biology Biological Techniques Celbiologie Biologische technieken dissertations. aat Academic theses fast Academic theses. lcgft http://id.loc.gov/authorities/genreForms/gf2014026039 Thèses et écrits académiques. rvmgf Internet Resources. Periasamy, Ammasi. http://id.loc.gov/authorities/names/n00014331 Day, Richard N. http://id.loc.gov/authorities/names/n2004018580 American Physiological Society (1887- ) http://id.loc.gov/authorities/names/n50060285 has work: Molecular imaging (Text) https://id.oclc.org/worldcat/entity/E39PCG9xYdvkRkyfR4vDxTBkWC https://id.oclc.org/worldcat/ontology/hasWork Print version: Molecular imaging. Oxford ; New York : Published for the American Physiological Society by Oxford University Press, 2005 0195177207 9780195177206 (DLC) 2004061702 (OCoLC)57406048 Methods in physiology series. http://id.loc.gov/authorities/names/n94036337 FWS01 ZDB-4-EBA FWS_PDA_EBA https://www.sciencedirect.com/science/book/9780195177206 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=210145 Volltext |
| spellingShingle | Molecular imaging : FRET microscopy and spectroscopy / Methods in physiology series. Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy -- Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization. Fluorescence microscopy. http://id.loc.gov/authorities/subjects/sh85049410 Fluorescence spectroscopy. http://id.loc.gov/authorities/subjects/sh85049411 Microscopy, Fluorescence Microscopy, Fluorescence methods Spectrometry, Fluorescence Spectrometry, Fluorescence methods Fluorescence Resonance Energy Transfer Microscopie de fluorescence. Spectroscopie de fluorescence. fluorescence microscopy. aat NATURE Reference. bisacsh SCIENCE Life Sciences General. bisacsh SCIENCE Life Sciences Biology. bisacsh Fluorescence microscopy fast Fluorescence spectroscopy fast |
| subject_GND | http://id.loc.gov/authorities/subjects/sh85049410 http://id.loc.gov/authorities/subjects/sh85049411 http://id.loc.gov/authorities/genreForms/gf2014026039 |
| title | Molecular imaging : FRET microscopy and spectroscopy / |
| title_auth | Molecular imaging : FRET microscopy and spectroscopy / |
| title_exact_search | Molecular imaging : FRET microscopy and spectroscopy / |
| title_full | Molecular imaging : FRET microscopy and spectroscopy / edited by Ammasi Periasamy, Richard N. Day. |
| title_fullStr | Molecular imaging : FRET microscopy and spectroscopy / edited by Ammasi Periasamy, Richard N. Day. |
| title_full_unstemmed | Molecular imaging : FRET microscopy and spectroscopy / edited by Ammasi Periasamy, Richard N. Day. |
| title_short | Molecular imaging : |
| title_sort | molecular imaging fret microscopy and spectroscopy |
| title_sub | FRET microscopy and spectroscopy / |
| topic | Fluorescence microscopy. http://id.loc.gov/authorities/subjects/sh85049410 Fluorescence spectroscopy. http://id.loc.gov/authorities/subjects/sh85049411 Microscopy, Fluorescence Microscopy, Fluorescence methods Spectrometry, Fluorescence Spectrometry, Fluorescence methods Fluorescence Resonance Energy Transfer Microscopie de fluorescence. Spectroscopie de fluorescence. fluorescence microscopy. aat NATURE Reference. bisacsh SCIENCE Life Sciences General. bisacsh SCIENCE Life Sciences Biology. bisacsh Fluorescence microscopy fast Fluorescence spectroscopy fast |
| topic_facet | Fluorescence microscopy. Fluorescence spectroscopy. Microscopy, Fluorescence Microscopy, Fluorescence methods Spectrometry, Fluorescence Spectrometry, Fluorescence methods Fluorescence Resonance Energy Transfer Microscopie de fluorescence. Spectroscopie de fluorescence. fluorescence microscopy. NATURE Reference. SCIENCE Life Sciences General. SCIENCE Life Sciences Biology. Fluorescence microscopy Fluorescence spectroscopy dissertations. Academic theses Academic theses. Thèses et écrits académiques. Internet Resources. |
| url | https://www.sciencedirect.com/science/book/9780195177206 https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=210145 |
| work_keys_str_mv | AT periasamyammasi molecularimagingfretmicroscopyandspectroscopy AT dayrichardn molecularimagingfretmicroscopyandspectroscopy AT americanphysiologicalsociety1887 molecularimagingfretmicroscopyandspectroscopy |