Arabidopsis :: a practical approach /
"Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Oxford ; New York :
Oxford University Press,
2000.
|
| Schriftenreihe: | Practical approach series ;
223. |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Zusammenfassung: | "Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source book for all researchers who work on Arabidopsis."--Jacket |
| Beschreibung: | 1 online resource (xix, 275 pages) : illustrations |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 0585483795 9780585483795 |
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| 505 | 0 | 0 | |g 1. |t Growth, maintenance, and use of Arabidopsis genetic resources / |r Mary Anderson, Fiona Wilson |g 1 -- |g 1. |t What is Arabidopsis? |g 1 -- |g 2. |t What makes Arabidopsis such an attractive experimental model? |g 3 -- |g 3. |t Arabidopsis genetic resource centres |g 4 -- |t Arabidopsis genetic resources |g 4 -- |t Accessing Arabidopsis resources |g 5 -- |g 4. |t Mutants of Arabidopsis |g 5 -- |t Single gene mutation lines |g 5 -- |t Resources for the identification/investigation of novel genes |g 8 -- |t Mapping tools |g 10 -- |g 5. |t Considerations of available resources for identifying novel genes |g 11 -- |t Forward genetics |g 12 -- |t Reverse genetics |g 14 -- |g 6. |t Growing Arabidopsis |g 15 -- |t How to maintain clean growth conditions |g 15 -- |t Growing Arabidopsis in the glasshouse |g 16 -- |t Chemical control of pests and diseases |g 19 -- |g 7. |t Seed storage |g 21 -- |g 8. |t Growing Arabidopsis with specific growth requirements |g 23 -- |g 9. |t Sterile culture of Arabidopsis |g 24 -- |g 2. |t Preservation and handling of stock centre clones / |r Randy Scholl, Keith Davis, Doreen Ware |g 29 -- |g 2. |t Missions of a plant DNA resource centre |g 29 -- |g 3. |t Preservation of stocks |g 30 -- |t Plasmids with small DNA inserts |g 30 -- |t Cosmids |g 32 -- |t Phage and phage libraries |g 33 -- |t Yeast artificial chromosome (YAC) libraries |g 33 -- |t Pools of YAC library cells for PCR screening |g 37 -- |t Distribution of YAC libraries arrayed on nylon filters |g 38 -- |t Other large-insert libraries |g 38 -- |t Yeast expression analysis--'two-hybrid' libraries and complementation testing |g 42 -- |g 4. |t Verification of stock identity and purity |g 42 -- |g 5. |t Pooled DNA from T-DNA lines for PCR screening |g 44 -- |g 6. |t Organization of stock information |g 48 -- |t Collecting, maintaining, and disseminating stock data |g 48 -- |t Organizing and distributing patron data |g 48 -- |g 7. |t Future |g 48 -- |g 3. |t Genetic mapping using recombinant inbred lines / |r Clare Lister, Mary Anderson, Caroline Dean |g 51 -- |g 2. |t Preparation and digestion of A. thaliana genomic DNA |g 54 -- |t Preparation of genomic DNA |g 56 -- |t Identifying an RFLP |g 60 -- |t Southern blotting and hybridization |g 61 -- |g 3. |t Polymorphic markers |g 62 -- |t RFLP markers |g 62 -- |t PCR-based markers |g 66 -- |t Phenotypic and biochemical markers |g 68 -- |g 4. |t Calculating map positions |g 69 -- |t Mapping programs |g 69 -- |t NASC mapping service |g 70 -- |g 5. |t Integration of a mutation into a molecular map |g 72 -- |g 4. |t Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / |r Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson |g 77 -- |g 1. |t Generation of mutants and their importance for developmental biology |g 77 -- |g 2. |t Mapping and segregation analysis |g 78 -- |t Mapping of mutations |g 78 -- |t Influence of environment on phenotype |g 82 -- |g 3. |t Microscopy |g 82 -- |t Fresh material characterization |g 82 -- |t Fixed material characterization |g 84 -- |g 4. |t Analysis of plant gene expression |g 89 -- |t RNA isolation |g 89 -- |t Northern analysis |g 91 -- |t In situ hybridizations |g 92 -- |g 5. |t Classical and molecular cytogenetics of Arabidopsis / |r G.H. Jones, J.S. Heslop-Harrison |g 105 -- |g 2. |t Mitotic chromosome analysis by light microscopy |g 108 -- |g 3. |t In situ hybridization to mitotic chromosome preparations |g 112 -- |t Photography of in situ hybridizations |g 117 -- |g 4. |t Meiotic chromosome analysis by light microscopy |g 117 -- |g 5. |t Meiotic chromosome analysis by electron microscopy |g 121 -- |g 6. |t Tissue culture, transformation, and transient gene expression in Arabidopsis / |r Keith Lindsey, Wenbin Wei |g 125 -- |g 2. |t Stable transformation by Agrobacterium tumefaciens |g 128 -- |g 3. |t Transient gene expression in Arabidopsis protoplasts |g 131 -- |t Reporter gene enzyme assays |g 134 -- |g 7. |t Transposon and T-DNA mutagenesis / |r Mark G.M. Aarts, Csaba Koncz, Andy Pereira |g 143 -- |g 2. |t Transposon tagging |g 143 -- |t Endogenous transposable elements |g 143 -- |t Transposon tagging systems in Arabidopsis |g 144 -- |t Which system to use? |g 149 -- |t Genetic and molecular analysis of a putatively transposon tagged mutant |g 151 -- |t Further applications of transposon tagging |g 156 -- |g 3.2 |t T-DNA tagging |g 158 -- |t Use of T-DNA as insertional mutagen |g 158 -- |t Random tagging |g 158 -- |t Available populations of T-DNA transformants |g 159 -- |t Promoter/enhancer trapping |g 160 -- |t Analysis of T-DNA mutants and cloning a tagged gene |g 161 -- |t Further applications of T-DNA tagging |g 166 -- |g 8. |t Map-based cloning in Arabidopsis / |r Joanna Putterill, George Coupland |g 171 -- |g 2. |t Locating the mutation of interest relative to DNA markers |g 172 -- |t Determining an approximate map position |g 172 -- |t Identifying a short genetic interval containing the mutation as a prelude to isolating the gene |g 175 -- |g 3. |t Placing the gene on the physical map |g 177 -- |t Chromosome landing |g 177 -- |t Chromosome walking with YAC clones |g 178 -- |g 4. |t Identification of the gene |g 189 -- |t Location of the gene by molecular complementation |g 189 -- |t Determining the structure of the gene |g 194 -- |g 5. |t Perspectives |g 194 -- |g 9. |t Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / |r Ian Bancroft |g 199 -- |g 2. |t Genome mapping with YAC clones |g 199 -- |g 3. |t Genome mapping with BAC and P1 clones |g 202 -- |t Communal resources |g 202 -- |t Construction of BAC libraries |g 208 -- |t Genome mapping with BACs |g 211 -- |g 4. |t High resolution mapping with cosmids |g 216 -- |t Approaches to mapping with cosmids |g 216 -- |t Construction of cosmid libraries |g 216 -- |g 5. |t Nucleotide sequences--the ultimate mapping tool |g 221 -- |t EST sequencing project |g 221 -- |t Genome sequencing project |g 221 -- |t Sequence-based mapping |g 222 -- |g 10. |t Web-based bioinformatic tools for Arabidopsis researchers / |r Seung Y. Rhee, David J. Flanders |g 225 -- |t What is bioinformatics |g 225 -- |t Sources of Arabidopsis bioinformatic data |g 226 -- |g 2. |t Basic tools for the Internet |g 227 -- |t Web basics |g 227 -- |t Getting onto the Web |g 228 -- |t Using your browser |g 229 -- |t Browser tips and errors |g 232 -- |t Privacy issues |g 235 -- |g 3. |t Scenarios of bioinformatic use in Arabidopsis research |g 235 -- |g 4. |t Gene information resources |g 237 -- |t General gene information |g 237 -- |t Arabidopsis gene information |g 237 -- |t Plant gene information |g 238 -- |t Genetic maps |g 239 -- |t Physical maps |g 240 -- |g 6. |t Sequencing |g 242 -- |t Arabidopsis genome initiative (AGI) |g 243 -- |t Annotation of sequences by AGI |g 243 -- |t Caveats in annotation |g 244 -- |t Sequence contigs from AtDB |g 245 -- |g 7. |t Sequence analysis tools |g 245 -- |t BLAST |g 246 -- |t FASTA |g 250 -- |t EST databases |g 252 -- |t Gene identification programs |g 253 -- |t Gene family analyses |g 255 -- |t Motif analyses |g 255 -- |t Protein structures |g 258 -- |t Comprehensive sequence analysis tools |g 259 -- |g 8. |t Current issues and future directions in bioinformatics |g 260 -- |t Some important bioinformatic issues |g 260 -- |t Bioinformatic tools currently under development for Arabidopsis research. |
| 520 | |a "Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source book for all researchers who work on Arabidopsis."--Jacket | ||
| 650 | 0 | |a Arabidopsis |v Laboratory manuals. | |
| 650 | 0 | |a Arabidopsis |x Molecular aspects |v Laboratory manuals. | |
| 650 | 1 | 2 | |a Arabidopsis |x genetics |0 https://id.nlm.nih.gov/mesh/D017360Q000235 |
| 650 | 2 | 2 | |a Plants, Genetically Modified |x genetics |0 https://id.nlm.nih.gov/mesh/D030821Q000235 |
| 650 | 6 | |a Arabidopsis |x Manuels de laboratoire. | |
| 650 | 6 | |a Arabidopsis |x Aspect moléculaire |x Manuels de laboratoire. | |
| 650 | 6 | |a Arabidopsis |v Manuels de laboratoire. | |
| 650 | 6 | |a Arabidopsis |x Aspect moléculaire |v Manuels de laboratoire. | |
| 650 | 7 | |a NATURE |x Plants |x General. |2 bisacsh | |
| 650 | 7 | |a Arabidopsis |2 fast | |
| 650 | 7 | |a Arabidopsis. |2 fmesh | |
| 655 | 2 | |a Laboratory Manual |0 https://id.nlm.nih.gov/mesh/D020484 | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals. |2 lcgft |0 http://id.loc.gov/authorities/genreForms/gf2014026120 | |
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| 700 | 1 | |a Wilson, Zoe A. | |
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Datensatz im Suchindex
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|---|---|
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| adam_text | |
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| author2 | Wilson, Zoe A. |
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| author_additional | Mary Anderson, Fiona Wilson Randy Scholl, Keith Davis, Doreen Ware Clare Lister, Mary Anderson, Caroline Dean Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson G.H. Jones, J.S. Heslop-Harrison Keith Lindsey, Wenbin Wei Mark G.M. Aarts, Csaba Koncz, Andy Pereira Joanna Putterill, George Coupland Ian Bancroft Seung Y. Rhee, David J. Flanders |
| author_facet | Wilson, Zoe A. |
| author_sort | Wilson, Zoe A. |
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| contents | Growth, maintenance, and use of Arabidopsis genetic resources / What is Arabidopsis? What makes Arabidopsis such an attractive experimental model? Arabidopsis genetic resource centres Arabidopsis genetic resources Accessing Arabidopsis resources Mutants of Arabidopsis Single gene mutation lines Resources for the identification/investigation of novel genes Mapping tools Considerations of available resources for identifying novel genes Forward genetics Reverse genetics Growing Arabidopsis How to maintain clean growth conditions Growing Arabidopsis in the glasshouse Chemical control of pests and diseases Seed storage Growing Arabidopsis with specific growth requirements Sterile culture of Arabidopsis Preservation and handling of stock centre clones / Missions of a plant DNA resource centre Preservation of stocks Plasmids with small DNA inserts Cosmids Phage and phage libraries Yeast artificial chromosome (YAC) libraries Pools of YAC library cells for PCR screening Distribution of YAC libraries arrayed on nylon filters Other large-insert libraries Yeast expression analysis--'two-hybrid' libraries and complementation testing Verification of stock identity and purity Pooled DNA from T-DNA lines for PCR screening Organization of stock information Collecting, maintaining, and disseminating stock data Organizing and distributing patron data Future Genetic mapping using recombinant inbred lines / Preparation and digestion of A. thaliana genomic DNA Preparation of genomic DNA Identifying an RFLP Southern blotting and hybridization Polymorphic markers RFLP markers PCR-based markers Phenotypic and biochemical markers Calculating map positions Mapping programs NASC mapping service Integration of a mutation into a molecular map Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / Generation of mutants and their importance for developmental biology Mapping and segregation analysis Mapping of mutations Influence of environment on phenotype Microscopy Fresh material characterization Fixed material characterization Analysis of plant gene expression RNA isolation Northern analysis In situ hybridizations Classical and molecular cytogenetics of Arabidopsis / Mitotic chromosome analysis by light microscopy In situ hybridization to mitotic chromosome preparations Photography of in situ hybridizations Meiotic chromosome analysis by light microscopy Meiotic chromosome analysis by electron microscopy Tissue culture, transformation, and transient gene expression in Arabidopsis / Stable transformation by Agrobacterium tumefaciens Transient gene expression in Arabidopsis protoplasts Reporter gene enzyme assays Transposon and T-DNA mutagenesis / Transposon tagging Endogenous transposable elements Transposon tagging systems in Arabidopsis Which system to use? Genetic and molecular analysis of a putatively transposon tagged mutant Further applications of transposon tagging T-DNA tagging Use of T-DNA as insertional mutagen Random tagging Available populations of T-DNA transformants Promoter/enhancer trapping Analysis of T-DNA mutants and cloning a tagged gene Further applications of T-DNA tagging Map-based cloning in Arabidopsis / Locating the mutation of interest relative to DNA markers Determining an approximate map position Identifying a short genetic interval containing the mutation as a prelude to isolating the gene Placing the gene on the physical map Chromosome landing Chromosome walking with YAC clones Identification of the gene Location of the gene by molecular complementation Determining the structure of the gene Perspectives Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / Genome mapping with YAC clones Genome mapping with BAC and P1 clones Communal resources Construction of BAC libraries Genome mapping with BACs High resolution mapping with cosmids Approaches to mapping with cosmids Construction of cosmid libraries Nucleotide sequences--the ultimate mapping tool EST sequencing project Genome sequencing project Sequence-based mapping Web-based bioinformatic tools for Arabidopsis researchers / What is bioinformatics Sources of Arabidopsis bioinformatic data Basic tools for the Internet Web basics Getting onto the Web Using your browser Browser tips and errors Privacy issues Scenarios of bioinformatic use in Arabidopsis research Gene information resources General gene information Arabidopsis gene information Plant gene information Genetic maps Physical maps Sequencing Arabidopsis genome initiative (AGI) Annotation of sequences by AGI Caveats in annotation Sequence contigs from AtDB Sequence analysis tools BLAST FASTA EST databases Gene identification programs Gene family analyses Motif analyses Protein structures Comprehensive sequence analysis tools Current issues and future directions in bioinformatics Some important bioinformatic issues Bioinformatic tools currently under development for Arabidopsis research. |
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| dewey-search | 583/.64 |
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| discipline | Biologie |
| format | Electronic eBook |
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centres</subfield><subfield code="g">4 --</subfield><subfield code="t">Arabidopsis genetic resources</subfield><subfield code="g">4 --</subfield><subfield code="t">Accessing Arabidopsis resources</subfield><subfield code="g">5 --</subfield><subfield code="g">4.</subfield><subfield code="t">Mutants of Arabidopsis</subfield><subfield code="g">5 --</subfield><subfield code="t">Single gene mutation lines</subfield><subfield code="g">5 --</subfield><subfield code="t">Resources for the identification/investigation of novel genes</subfield><subfield code="g">8 --</subfield><subfield code="t">Mapping tools</subfield><subfield code="g">10 --</subfield><subfield code="g">5.</subfield><subfield code="t">Considerations of available resources for identifying novel genes</subfield><subfield code="g">11 --</subfield><subfield code="t">Forward genetics</subfield><subfield code="g">12 --</subfield><subfield code="t">Reverse genetics</subfield><subfield code="g">14 --</subfield><subfield code="g">6.</subfield><subfield code="t">Growing Arabidopsis</subfield><subfield code="g">15 --</subfield><subfield code="t">How to maintain clean growth conditions</subfield><subfield code="g">15 --</subfield><subfield code="t">Growing Arabidopsis in the glasshouse</subfield><subfield code="g">16 --</subfield><subfield code="t">Chemical control of pests and diseases</subfield><subfield code="g">19 --</subfield><subfield code="g">7.</subfield><subfield code="t">Seed storage</subfield><subfield code="g">21 --</subfield><subfield code="g">8.</subfield><subfield code="t">Growing Arabidopsis with specific growth requirements</subfield><subfield code="g">23 --</subfield><subfield code="g">9.</subfield><subfield code="t">Sterile culture of Arabidopsis</subfield><subfield code="g">24 --</subfield><subfield code="g">2.</subfield><subfield code="t">Preservation and handling of stock centre clones /</subfield><subfield code="r">Randy Scholl, Keith Davis, Doreen Ware</subfield><subfield code="g">29 --</subfield><subfield code="g">2.</subfield><subfield code="t">Missions of a plant DNA resource centre</subfield><subfield code="g">29 --</subfield><subfield code="g">3.</subfield><subfield code="t">Preservation of stocks</subfield><subfield code="g">30 --</subfield><subfield code="t">Plasmids with small DNA inserts</subfield><subfield code="g">30 --</subfield><subfield code="t">Cosmids</subfield><subfield code="g">32 --</subfield><subfield code="t">Phage and phage libraries</subfield><subfield code="g">33 --</subfield><subfield code="t">Yeast artificial chromosome (YAC) libraries</subfield><subfield code="g">33 --</subfield><subfield code="t">Pools of YAC library cells for PCR screening</subfield><subfield code="g">37 --</subfield><subfield code="t">Distribution of YAC libraries arrayed on nylon filters</subfield><subfield code="g">38 --</subfield><subfield code="t">Other large-insert libraries</subfield><subfield code="g">38 --</subfield><subfield code="t">Yeast expression analysis--'two-hybrid' libraries and complementation testing</subfield><subfield code="g">42 --</subfield><subfield code="g">4.</subfield><subfield code="t">Verification of stock identity and purity</subfield><subfield code="g">42 --</subfield><subfield code="g">5.</subfield><subfield code="t">Pooled DNA from T-DNA lines for PCR screening</subfield><subfield code="g">44 --</subfield><subfield code="g">6.</subfield><subfield code="t">Organization of stock information</subfield><subfield code="g">48 --</subfield><subfield code="t">Collecting, maintaining, and disseminating stock data</subfield><subfield code="g">48 --</subfield><subfield code="t">Organizing and distributing patron data</subfield><subfield code="g">48 --</subfield><subfield code="g">7.</subfield><subfield code="t">Future</subfield><subfield code="g">48 --</subfield><subfield code="g">3.</subfield><subfield code="t">Genetic mapping using recombinant inbred lines /</subfield><subfield code="r">Clare Lister, Mary Anderson, Caroline Dean</subfield><subfield code="g">51 --</subfield><subfield code="g">2.</subfield><subfield code="t">Preparation and digestion of A. thaliana genomic DNA</subfield><subfield code="g">54 --</subfield><subfield code="t">Preparation of genomic DNA</subfield><subfield code="g">56 --</subfield><subfield code="t">Identifying an RFLP</subfield><subfield code="g">60 --</subfield><subfield code="t">Southern blotting and hybridization</subfield><subfield code="g">61 --</subfield><subfield code="g">3.</subfield><subfield code="t">Polymorphic markers</subfield><subfield code="g">62 --</subfield><subfield code="t">RFLP markers</subfield><subfield code="g">62 --</subfield><subfield code="t">PCR-based markers</subfield><subfield code="g">66 --</subfield><subfield code="t">Phenotypic and biochemical markers</subfield><subfield code="g">68 --</subfield><subfield code="g">4.</subfield><subfield code="t">Calculating map positions</subfield><subfield code="g">69 --</subfield><subfield code="t">Mapping programs</subfield><subfield code="g">69 --</subfield><subfield code="t">NASC mapping service</subfield><subfield code="g">70 --</subfield><subfield code="g">5.</subfield><subfield code="t">Integration of a mutation into a molecular map</subfield><subfield code="g">72 --</subfield><subfield code="g">4.</subfield><subfield code="t">Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis /</subfield><subfield code="r">Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson</subfield><subfield code="g">77 --</subfield><subfield code="g">1.</subfield><subfield code="t">Generation of mutants and their importance for developmental biology</subfield><subfield code="g">77 --</subfield><subfield code="g">2.</subfield><subfield code="t">Mapping and segregation analysis</subfield><subfield code="g">78 --</subfield><subfield code="t">Mapping of mutations</subfield><subfield code="g">78 --</subfield><subfield code="t">Influence of environment on phenotype</subfield><subfield code="g">82 --</subfield><subfield code="g">3.</subfield><subfield code="t">Microscopy</subfield><subfield code="g">82 --</subfield><subfield code="t">Fresh material characterization</subfield><subfield code="g">82 --</subfield><subfield code="t">Fixed material characterization</subfield><subfield code="g">84 --</subfield><subfield code="g">4.</subfield><subfield code="t">Analysis of plant gene expression</subfield><subfield code="g">89 --</subfield><subfield code="t">RNA isolation</subfield><subfield code="g">89 --</subfield><subfield code="t">Northern analysis</subfield><subfield code="g">91 --</subfield><subfield code="t">In situ hybridizations</subfield><subfield code="g">92 --</subfield><subfield code="g">5.</subfield><subfield code="t">Classical and molecular cytogenetics of Arabidopsis /</subfield><subfield code="r">G.H. Jones, J.S. Heslop-Harrison</subfield><subfield code="g">105 --</subfield><subfield code="g">2.</subfield><subfield code="t">Mitotic chromosome analysis by light microscopy</subfield><subfield code="g">108 --</subfield><subfield code="g">3.</subfield><subfield code="t">In situ hybridization to mitotic chromosome preparations</subfield><subfield code="g">112 --</subfield><subfield code="t">Photography of in situ hybridizations</subfield><subfield code="g">117 --</subfield><subfield code="g">4.</subfield><subfield code="t">Meiotic chromosome analysis by light microscopy</subfield><subfield code="g">117 --</subfield><subfield code="g">5.</subfield><subfield code="t">Meiotic chromosome analysis by electron microscopy</subfield><subfield code="g">121 --</subfield><subfield code="g">6.</subfield><subfield code="t">Tissue culture, transformation, and transient gene expression in Arabidopsis /</subfield><subfield code="r">Keith Lindsey, Wenbin Wei</subfield><subfield code="g">125 --</subfield><subfield code="g">2.</subfield><subfield code="t">Stable transformation by Agrobacterium tumefaciens</subfield><subfield code="g">128 --</subfield><subfield code="g">3.</subfield><subfield code="t">Transient gene expression in Arabidopsis protoplasts</subfield><subfield code="g">131 --</subfield><subfield code="t">Reporter gene enzyme assays</subfield><subfield code="g">134 --</subfield><subfield code="g">7.</subfield><subfield code="t">Transposon and T-DNA mutagenesis /</subfield><subfield code="r">Mark G.M. Aarts, Csaba Koncz, Andy Pereira</subfield><subfield code="g">143 --</subfield><subfield code="g">2.</subfield><subfield code="t">Transposon tagging</subfield><subfield code="g">143 --</subfield><subfield code="t">Endogenous transposable elements</subfield><subfield code="g">143 --</subfield><subfield code="t">Transposon tagging systems in Arabidopsis</subfield><subfield code="g">144 --</subfield><subfield code="t">Which system to use?</subfield><subfield code="g">149 --</subfield><subfield code="t">Genetic and molecular analysis of a putatively transposon tagged mutant</subfield><subfield code="g">151 --</subfield><subfield code="t">Further applications of transposon tagging</subfield><subfield code="g">156 --</subfield><subfield code="g">3.2</subfield><subfield code="t">T-DNA tagging</subfield><subfield code="g">158 --</subfield><subfield code="t">Use of T-DNA as insertional mutagen</subfield><subfield code="g">158 --</subfield><subfield code="t">Random tagging</subfield><subfield code="g">158 --</subfield><subfield code="t">Available populations of T-DNA transformants</subfield><subfield code="g">159 --</subfield><subfield code="t">Promoter/enhancer trapping</subfield><subfield code="g">160 --</subfield><subfield code="t">Analysis of T-DNA mutants and cloning a tagged gene</subfield><subfield code="g">161 --</subfield><subfield code="t">Further applications of T-DNA tagging</subfield><subfield code="g">166 --</subfield><subfield code="g">8.</subfield><subfield code="t">Map-based cloning in Arabidopsis /</subfield><subfield code="r">Joanna Putterill, George Coupland</subfield><subfield code="g">171 --</subfield><subfield code="g">2.</subfield><subfield code="t">Locating the mutation of interest relative to DNA markers</subfield><subfield code="g">172 --</subfield><subfield code="t">Determining an approximate map position</subfield><subfield code="g">172 --</subfield><subfield code="t">Identifying a short genetic interval containing the mutation as a prelude to isolating the gene</subfield><subfield code="g">175 --</subfield><subfield code="g">3.</subfield><subfield code="t">Placing the gene on the physical map</subfield><subfield code="g">177 --</subfield><subfield code="t">Chromosome landing</subfield><subfield code="g">177 --</subfield><subfield code="t">Chromosome walking with YAC clones</subfield><subfield code="g">178 --</subfield><subfield code="g">4.</subfield><subfield code="t">Identification of the gene</subfield><subfield code="g">189 --</subfield><subfield code="t">Location of the gene by molecular complementation</subfield><subfield code="g">189 --</subfield><subfield code="t">Determining the structure of the gene</subfield><subfield code="g">194 --</subfield><subfield code="g">5.</subfield><subfield code="t">Perspectives</subfield><subfield code="g">194 --</subfield><subfield code="g">9.</subfield><subfield code="t">Physical mapping: YACs, BACs, cosmids, and nucleotide sequences /</subfield><subfield code="r">Ian Bancroft</subfield><subfield code="g">199 --</subfield><subfield code="g">2.</subfield><subfield code="t">Genome mapping with YAC clones</subfield><subfield code="g">199 --</subfield><subfield code="g">3.</subfield><subfield code="t">Genome mapping with BAC and P1 clones</subfield><subfield code="g">202 --</subfield><subfield code="t">Communal resources</subfield><subfield code="g">202 --</subfield><subfield code="t">Construction of BAC libraries</subfield><subfield code="g">208 --</subfield><subfield code="t">Genome mapping with BACs</subfield><subfield code="g">211 --</subfield><subfield code="g">4.</subfield><subfield code="t">High resolution mapping with cosmids</subfield><subfield code="g">216 --</subfield><subfield code="t">Approaches to mapping with cosmids</subfield><subfield code="g">216 --</subfield><subfield code="t">Construction of cosmid libraries</subfield><subfield code="g">216 --</subfield><subfield code="g">5.</subfield><subfield code="t">Nucleotide sequences--the ultimate mapping tool</subfield><subfield code="g">221 --</subfield><subfield code="t">EST sequencing project</subfield><subfield code="g">221 --</subfield><subfield code="t">Genome sequencing project</subfield><subfield code="g">221 --</subfield><subfield code="t">Sequence-based mapping</subfield><subfield code="g">222 --</subfield><subfield code="g">10.</subfield><subfield code="t">Web-based bioinformatic tools for Arabidopsis researchers /</subfield><subfield code="r">Seung Y. Rhee, David J. Flanders</subfield><subfield code="g">225 --</subfield><subfield code="t">What is bioinformatics</subfield><subfield code="g">225 --</subfield><subfield code="t">Sources of Arabidopsis bioinformatic data</subfield><subfield code="g">226 --</subfield><subfield code="g">2.</subfield><subfield code="t">Basic tools for the Internet</subfield><subfield code="g">227 --</subfield><subfield code="t">Web basics</subfield><subfield code="g">227 --</subfield><subfield code="t">Getting onto the Web</subfield><subfield code="g">228 --</subfield><subfield code="t">Using your browser</subfield><subfield code="g">229 --</subfield><subfield code="t">Browser tips and errors</subfield><subfield code="g">232 --</subfield><subfield code="t">Privacy issues</subfield><subfield code="g">235 --</subfield><subfield code="g">3.</subfield><subfield code="t">Scenarios of bioinformatic use in Arabidopsis research</subfield><subfield code="g">235 --</subfield><subfield code="g">4.</subfield><subfield code="t">Gene information resources</subfield><subfield code="g">237 --</subfield><subfield code="t">General gene information</subfield><subfield code="g">237 --</subfield><subfield code="t">Arabidopsis gene information</subfield><subfield code="g">237 --</subfield><subfield code="t">Plant gene information</subfield><subfield code="g">238 --</subfield><subfield code="t">Genetic maps</subfield><subfield code="g">239 --</subfield><subfield code="t">Physical maps</subfield><subfield code="g">240 --</subfield><subfield code="g">6.</subfield><subfield code="t">Sequencing</subfield><subfield code="g">242 --</subfield><subfield code="t">Arabidopsis genome initiative (AGI)</subfield><subfield code="g">243 --</subfield><subfield code="t">Annotation of sequences by AGI</subfield><subfield code="g">243 --</subfield><subfield code="t">Caveats in annotation</subfield><subfield code="g">244 --</subfield><subfield code="t">Sequence contigs from AtDB</subfield><subfield code="g">245 --</subfield><subfield code="g">7.</subfield><subfield code="t">Sequence analysis tools</subfield><subfield code="g">245 --</subfield><subfield code="t">BLAST</subfield><subfield code="g">246 --</subfield><subfield code="t">FASTA</subfield><subfield code="g">250 --</subfield><subfield code="t">EST databases</subfield><subfield code="g">252 --</subfield><subfield code="t">Gene identification programs</subfield><subfield code="g">253 --</subfield><subfield code="t">Gene family analyses</subfield><subfield code="g">255 --</subfield><subfield code="t">Motif analyses</subfield><subfield code="g">255 --</subfield><subfield code="t">Protein structures</subfield><subfield code="g">258 --</subfield><subfield code="t">Comprehensive sequence analysis tools</subfield><subfield code="g">259 --</subfield><subfield code="g">8.</subfield><subfield code="t">Current issues and future directions in bioinformatics</subfield><subfield code="g">260 --</subfield><subfield code="t">Some important bioinformatic issues</subfield><subfield code="g">260 --</subfield><subfield code="t">Bioinformatic tools currently under development for Arabidopsis research.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">"Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source book for all researchers who work on Arabidopsis."--Jacket</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Arabidopsis</subfield><subfield code="v">Laboratory manuals.</subfield></datafield><datafield tag="650" ind1=" " ind2="0"><subfield code="a">Arabidopsis</subfield><subfield code="x">Molecular aspects</subfield><subfield code="v">Laboratory manuals.</subfield></datafield><datafield tag="650" ind1="1" ind2="2"><subfield code="a">Arabidopsis</subfield><subfield code="x">genetics</subfield><subfield code="0">https://id.nlm.nih.gov/mesh/D017360Q000235</subfield></datafield><datafield tag="650" ind1="2" ind2="2"><subfield code="a">Plants, Genetically Modified</subfield><subfield code="x">genetics</subfield><subfield code="0">https://id.nlm.nih.gov/mesh/D030821Q000235</subfield></datafield><datafield tag="650" ind1=" " ind2="6"><subfield code="a">Arabidopsis</subfield><subfield code="x">Manuels de laboratoire.</subfield></datafield><datafield tag="650" ind1=" " ind2="6"><subfield code="a">Arabidopsis</subfield><subfield code="x">Aspect moléculaire</subfield><subfield code="x">Manuels de laboratoire.</subfield></datafield><datafield tag="650" ind1=" " ind2="6"><subfield code="a">Arabidopsis</subfield><subfield code="v">Manuels de laboratoire.</subfield></datafield><datafield tag="650" ind1=" " ind2="6"><subfield code="a">Arabidopsis</subfield><subfield code="x">Aspect moléculaire</subfield><subfield code="v">Manuels de laboratoire.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NATURE</subfield><subfield code="x">Plants</subfield><subfield code="x">General.</subfield><subfield code="2">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Arabidopsis</subfield><subfield code="2">fast</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Arabidopsis.</subfield><subfield code="2">fmesh</subfield></datafield><datafield tag="655" ind1=" " ind2="2"><subfield code="a">Laboratory Manual</subfield><subfield code="0">https://id.nlm.nih.gov/mesh/D020484</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="a">Laboratory manuals</subfield><subfield code="2">fast</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="a">Laboratory manuals.</subfield><subfield code="2">lcgft</subfield><subfield code="0">http://id.loc.gov/authorities/genreForms/gf2014026120</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="a">Manuels de laboratoire.</subfield><subfield code="2">rvmgf</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wilson, Zoe A.</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Print version:</subfield><subfield code="t">Arabidopsis.</subfield><subfield code="d">Oxford ; New York : Oxford University Press, 2000</subfield><subfield code="z">019963565X</subfield><subfield code="z">0199635641</subfield><subfield code="w">(DLC) 99045851</subfield><subfield code="w">(OCoLC)42295871</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Practical approach series ;</subfield><subfield code="v">223.</subfield><subfield code="0">http://id.loc.gov/authorities/names/n84709852</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="l">FWS01</subfield><subfield code="p">ZDB-4-EBA</subfield><subfield code="q">FWS_PDA_EBA</subfield><subfield code="u">https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98516</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">YBP Library Services</subfield><subfield code="b">YANK</subfield><subfield code="n">20452287</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">ProQuest Ebook Central</subfield><subfield code="b">EBLB</subfield><subfield code="n">EBL5583491</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">EBSCOhost</subfield><subfield code="b">EBSC</subfield><subfield code="n">98516</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">Internet Archive</subfield><subfield code="b">INAR</subfield><subfield code="n">arabidopsis00zoea</subfield></datafield><datafield tag="938" ind1=" " ind2=" "><subfield code="a">YBP Library Services</subfield><subfield code="b">YANK</subfield><subfield code="n">2336205</subfield></datafield><datafield tag="994" ind1=" " ind2=" "><subfield code="a">92</subfield><subfield code="b">GEBAY</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-4-EBA</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-863</subfield></datafield></record></collection> |
| genre | Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Manuels de laboratoire. rvmgf |
| genre_facet | Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| id | ZDB-4-EBA-ocm53982369 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:15:30Z |
| institution | BVB |
| isbn | 0585483795 9780585483795 |
| language | English |
| oclc_num | 53982369 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xix, 275 pages) : illustrations |
| psigel | ZDB-4-EBA |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Oxford University Press, |
| record_format | marc |
| series | Practical approach series ; |
| series2 | The practical approach series ; |
| spelling | Arabidopsis : a practical approach / edited by Zoe A. Wilson. Oxford ; New York : Oxford University Press, 2000. 1 online resource (xix, 275 pages) : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier data file The practical approach series ; PAS/223 Includes bibliographical references and index. Print version record. 1. Growth, maintenance, and use of Arabidopsis genetic resources / Mary Anderson, Fiona Wilson 1 -- 1. What is Arabidopsis? 1 -- 2. What makes Arabidopsis such an attractive experimental model? 3 -- 3. Arabidopsis genetic resource centres 4 -- Arabidopsis genetic resources 4 -- Accessing Arabidopsis resources 5 -- 4. Mutants of Arabidopsis 5 -- Single gene mutation lines 5 -- Resources for the identification/investigation of novel genes 8 -- Mapping tools 10 -- 5. Considerations of available resources for identifying novel genes 11 -- Forward genetics 12 -- Reverse genetics 14 -- 6. Growing Arabidopsis 15 -- How to maintain clean growth conditions 15 -- Growing Arabidopsis in the glasshouse 16 -- Chemical control of pests and diseases 19 -- 7. Seed storage 21 -- 8. Growing Arabidopsis with specific growth requirements 23 -- 9. Sterile culture of Arabidopsis 24 -- 2. Preservation and handling of stock centre clones / Randy Scholl, Keith Davis, Doreen Ware 29 -- 2. Missions of a plant DNA resource centre 29 -- 3. Preservation of stocks 30 -- Plasmids with small DNA inserts 30 -- Cosmids 32 -- Phage and phage libraries 33 -- Yeast artificial chromosome (YAC) libraries 33 -- Pools of YAC library cells for PCR screening 37 -- Distribution of YAC libraries arrayed on nylon filters 38 -- Other large-insert libraries 38 -- Yeast expression analysis--'two-hybrid' libraries and complementation testing 42 -- 4. Verification of stock identity and purity 42 -- 5. Pooled DNA from T-DNA lines for PCR screening 44 -- 6. Organization of stock information 48 -- Collecting, maintaining, and disseminating stock data 48 -- Organizing and distributing patron data 48 -- 7. Future 48 -- 3. Genetic mapping using recombinant inbred lines / Clare Lister, Mary Anderson, Caroline Dean 51 -- 2. Preparation and digestion of A. thaliana genomic DNA 54 -- Preparation of genomic DNA 56 -- Identifying an RFLP 60 -- Southern blotting and hybridization 61 -- 3. Polymorphic markers 62 -- RFLP markers 62 -- PCR-based markers 66 -- Phenotypic and biochemical markers 68 -- 4. Calculating map positions 69 -- Mapping programs 69 -- NASC mapping service 70 -- 5. Integration of a mutation into a molecular map 72 -- 4. Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / Kriton Kalantidis, L. Greg Briarty, Zoe A. Wilson 77 -- 1. Generation of mutants and their importance for developmental biology 77 -- 2. Mapping and segregation analysis 78 -- Mapping of mutations 78 -- Influence of environment on phenotype 82 -- 3. Microscopy 82 -- Fresh material characterization 82 -- Fixed material characterization 84 -- 4. Analysis of plant gene expression 89 -- RNA isolation 89 -- Northern analysis 91 -- In situ hybridizations 92 -- 5. Classical and molecular cytogenetics of Arabidopsis / G.H. Jones, J.S. Heslop-Harrison 105 -- 2. Mitotic chromosome analysis by light microscopy 108 -- 3. In situ hybridization to mitotic chromosome preparations 112 -- Photography of in situ hybridizations 117 -- 4. Meiotic chromosome analysis by light microscopy 117 -- 5. Meiotic chromosome analysis by electron microscopy 121 -- 6. Tissue culture, transformation, and transient gene expression in Arabidopsis / Keith Lindsey, Wenbin Wei 125 -- 2. Stable transformation by Agrobacterium tumefaciens 128 -- 3. Transient gene expression in Arabidopsis protoplasts 131 -- Reporter gene enzyme assays 134 -- 7. Transposon and T-DNA mutagenesis / Mark G.M. Aarts, Csaba Koncz, Andy Pereira 143 -- 2. Transposon tagging 143 -- Endogenous transposable elements 143 -- Transposon tagging systems in Arabidopsis 144 -- Which system to use? 149 -- Genetic and molecular analysis of a putatively transposon tagged mutant 151 -- Further applications of transposon tagging 156 -- 3.2 T-DNA tagging 158 -- Use of T-DNA as insertional mutagen 158 -- Random tagging 158 -- Available populations of T-DNA transformants 159 -- Promoter/enhancer trapping 160 -- Analysis of T-DNA mutants and cloning a tagged gene 161 -- Further applications of T-DNA tagging 166 -- 8. Map-based cloning in Arabidopsis / Joanna Putterill, George Coupland 171 -- 2. Locating the mutation of interest relative to DNA markers 172 -- Determining an approximate map position 172 -- Identifying a short genetic interval containing the mutation as a prelude to isolating the gene 175 -- 3. Placing the gene on the physical map 177 -- Chromosome landing 177 -- Chromosome walking with YAC clones 178 -- 4. Identification of the gene 189 -- Location of the gene by molecular complementation 189 -- Determining the structure of the gene 194 -- 5. Perspectives 194 -- 9. Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / Ian Bancroft 199 -- 2. Genome mapping with YAC clones 199 -- 3. Genome mapping with BAC and P1 clones 202 -- Communal resources 202 -- Construction of BAC libraries 208 -- Genome mapping with BACs 211 -- 4. High resolution mapping with cosmids 216 -- Approaches to mapping with cosmids 216 -- Construction of cosmid libraries 216 -- 5. Nucleotide sequences--the ultimate mapping tool 221 -- EST sequencing project 221 -- Genome sequencing project 221 -- Sequence-based mapping 222 -- 10. Web-based bioinformatic tools for Arabidopsis researchers / Seung Y. Rhee, David J. Flanders 225 -- What is bioinformatics 225 -- Sources of Arabidopsis bioinformatic data 226 -- 2. Basic tools for the Internet 227 -- Web basics 227 -- Getting onto the Web 228 -- Using your browser 229 -- Browser tips and errors 232 -- Privacy issues 235 -- 3. Scenarios of bioinformatic use in Arabidopsis research 235 -- 4. Gene information resources 237 -- General gene information 237 -- Arabidopsis gene information 237 -- Plant gene information 238 -- Genetic maps 239 -- Physical maps 240 -- 6. Sequencing 242 -- Arabidopsis genome initiative (AGI) 243 -- Annotation of sequences by AGI 243 -- Caveats in annotation 244 -- Sequence contigs from AtDB 245 -- 7. Sequence analysis tools 245 -- BLAST 246 -- FASTA 250 -- EST databases 252 -- Gene identification programs 253 -- Gene family analyses 255 -- Motif analyses 255 -- Protein structures 258 -- Comprehensive sequence analysis tools 259 -- 8. Current issues and future directions in bioinformatics 260 -- Some important bioinformatic issues 260 -- Bioinformatic tools currently under development for Arabidopsis research. "Arabidopsis has long been acknowledged as the ideal molecular genetic tool for the analysis of development in higher plants. This book covers all the key techniques for using Arabidopsis as an experimental system. This fully up-to-date and comprehensive laboratory manual is an essential source book for all researchers who work on Arabidopsis."--Jacket Arabidopsis Laboratory manuals. Arabidopsis Molecular aspects Laboratory manuals. Arabidopsis genetics https://id.nlm.nih.gov/mesh/D017360Q000235 Plants, Genetically Modified genetics https://id.nlm.nih.gov/mesh/D030821Q000235 Arabidopsis Manuels de laboratoire. Arabidopsis Aspect moléculaire Manuels de laboratoire. NATURE Plants General. bisacsh Arabidopsis fast Arabidopsis. fmesh Laboratory Manual https://id.nlm.nih.gov/mesh/D020484 Laboratory manuals fast Laboratory manuals. lcgft http://id.loc.gov/authorities/genreForms/gf2014026120 Manuels de laboratoire. rvmgf Wilson, Zoe A. Print version: Arabidopsis. Oxford ; New York : Oxford University Press, 2000 019963565X 0199635641 (DLC) 99045851 (OCoLC)42295871 Practical approach series ; 223. http://id.loc.gov/authorities/names/n84709852 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98516 Volltext |
| spellingShingle | Arabidopsis : a practical approach / Practical approach series ; Growth, maintenance, and use of Arabidopsis genetic resources / What is Arabidopsis? What makes Arabidopsis such an attractive experimental model? Arabidopsis genetic resource centres Arabidopsis genetic resources Accessing Arabidopsis resources Mutants of Arabidopsis Single gene mutation lines Resources for the identification/investigation of novel genes Mapping tools Considerations of available resources for identifying novel genes Forward genetics Reverse genetics Growing Arabidopsis How to maintain clean growth conditions Growing Arabidopsis in the glasshouse Chemical control of pests and diseases Seed storage Growing Arabidopsis with specific growth requirements Sterile culture of Arabidopsis Preservation and handling of stock centre clones / Missions of a plant DNA resource centre Preservation of stocks Plasmids with small DNA inserts Cosmids Phage and phage libraries Yeast artificial chromosome (YAC) libraries Pools of YAC library cells for PCR screening Distribution of YAC libraries arrayed on nylon filters Other large-insert libraries Yeast expression analysis--'two-hybrid' libraries and complementation testing Verification of stock identity and purity Pooled DNA from T-DNA lines for PCR screening Organization of stock information Collecting, maintaining, and disseminating stock data Organizing and distributing patron data Future Genetic mapping using recombinant inbred lines / Preparation and digestion of A. thaliana genomic DNA Preparation of genomic DNA Identifying an RFLP Southern blotting and hybridization Polymorphic markers RFLP markers PCR-based markers Phenotypic and biochemical markers Calculating map positions Mapping programs NASC mapping service Integration of a mutation into a molecular map Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / Generation of mutants and their importance for developmental biology Mapping and segregation analysis Mapping of mutations Influence of environment on phenotype Microscopy Fresh material characterization Fixed material characterization Analysis of plant gene expression RNA isolation Northern analysis In situ hybridizations Classical and molecular cytogenetics of Arabidopsis / Mitotic chromosome analysis by light microscopy In situ hybridization to mitotic chromosome preparations Photography of in situ hybridizations Meiotic chromosome analysis by light microscopy Meiotic chromosome analysis by electron microscopy Tissue culture, transformation, and transient gene expression in Arabidopsis / Stable transformation by Agrobacterium tumefaciens Transient gene expression in Arabidopsis protoplasts Reporter gene enzyme assays Transposon and T-DNA mutagenesis / Transposon tagging Endogenous transposable elements Transposon tagging systems in Arabidopsis Which system to use? Genetic and molecular analysis of a putatively transposon tagged mutant Further applications of transposon tagging T-DNA tagging Use of T-DNA as insertional mutagen Random tagging Available populations of T-DNA transformants Promoter/enhancer trapping Analysis of T-DNA mutants and cloning a tagged gene Further applications of T-DNA tagging Map-based cloning in Arabidopsis / Locating the mutation of interest relative to DNA markers Determining an approximate map position Identifying a short genetic interval containing the mutation as a prelude to isolating the gene Placing the gene on the physical map Chromosome landing Chromosome walking with YAC clones Identification of the gene Location of the gene by molecular complementation Determining the structure of the gene Perspectives Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / Genome mapping with YAC clones Genome mapping with BAC and P1 clones Communal resources Construction of BAC libraries Genome mapping with BACs High resolution mapping with cosmids Approaches to mapping with cosmids Construction of cosmid libraries Nucleotide sequences--the ultimate mapping tool EST sequencing project Genome sequencing project Sequence-based mapping Web-based bioinformatic tools for Arabidopsis researchers / What is bioinformatics Sources of Arabidopsis bioinformatic data Basic tools for the Internet Web basics Getting onto the Web Using your browser Browser tips and errors Privacy issues Scenarios of bioinformatic use in Arabidopsis research Gene information resources General gene information Arabidopsis gene information Plant gene information Genetic maps Physical maps Sequencing Arabidopsis genome initiative (AGI) Annotation of sequences by AGI Caveats in annotation Sequence contigs from AtDB Sequence analysis tools BLAST FASTA EST databases Gene identification programs Gene family analyses Motif analyses Protein structures Comprehensive sequence analysis tools Current issues and future directions in bioinformatics Some important bioinformatic issues Bioinformatic tools currently under development for Arabidopsis research. Arabidopsis Laboratory manuals. Arabidopsis Molecular aspects Laboratory manuals. Arabidopsis genetics https://id.nlm.nih.gov/mesh/D017360Q000235 Plants, Genetically Modified genetics https://id.nlm.nih.gov/mesh/D030821Q000235 Arabidopsis Manuels de laboratoire. Arabidopsis Aspect moléculaire Manuels de laboratoire. NATURE Plants General. bisacsh Arabidopsis fast Arabidopsis. fmesh |
| subject_GND | https://id.nlm.nih.gov/mesh/D017360Q000235 https://id.nlm.nih.gov/mesh/D030821Q000235 https://id.nlm.nih.gov/mesh/D020484 http://id.loc.gov/authorities/genreForms/gf2014026120 |
| title | Arabidopsis : a practical approach / |
| title_alt | Growth, maintenance, and use of Arabidopsis genetic resources / What is Arabidopsis? What makes Arabidopsis such an attractive experimental model? Arabidopsis genetic resource centres Arabidopsis genetic resources Accessing Arabidopsis resources Mutants of Arabidopsis Single gene mutation lines Resources for the identification/investigation of novel genes Mapping tools Considerations of available resources for identifying novel genes Forward genetics Reverse genetics Growing Arabidopsis How to maintain clean growth conditions Growing Arabidopsis in the glasshouse Chemical control of pests and diseases Seed storage Growing Arabidopsis with specific growth requirements Sterile culture of Arabidopsis Preservation and handling of stock centre clones / Missions of a plant DNA resource centre Preservation of stocks Plasmids with small DNA inserts Cosmids Phage and phage libraries Yeast artificial chromosome (YAC) libraries Pools of YAC library cells for PCR screening Distribution of YAC libraries arrayed on nylon filters Other large-insert libraries Yeast expression analysis--'two-hybrid' libraries and complementation testing Verification of stock identity and purity Pooled DNA from T-DNA lines for PCR screening Organization of stock information Collecting, maintaining, and disseminating stock data Organizing and distributing patron data Future Genetic mapping using recombinant inbred lines / Preparation and digestion of A. thaliana genomic DNA Preparation of genomic DNA Identifying an RFLP Southern blotting and hybridization Polymorphic markers RFLP markers PCR-based markers Phenotypic and biochemical markers Calculating map positions Mapping programs NASC mapping service Integration of a mutation into a molecular map Arabidopsis mutant characterization; microscopy, mapping, and gene expression analysis / Generation of mutants and their importance for developmental biology Mapping and segregation analysis Mapping of mutations Influence of environment on phenotype Microscopy Fresh material characterization Fixed material characterization Analysis of plant gene expression RNA isolation Northern analysis In situ hybridizations Classical and molecular cytogenetics of Arabidopsis / Mitotic chromosome analysis by light microscopy In situ hybridization to mitotic chromosome preparations Photography of in situ hybridizations Meiotic chromosome analysis by light microscopy Meiotic chromosome analysis by electron microscopy Tissue culture, transformation, and transient gene expression in Arabidopsis / Stable transformation by Agrobacterium tumefaciens Transient gene expression in Arabidopsis protoplasts Reporter gene enzyme assays Transposon and T-DNA mutagenesis / Transposon tagging Endogenous transposable elements Transposon tagging systems in Arabidopsis Which system to use? Genetic and molecular analysis of a putatively transposon tagged mutant Further applications of transposon tagging T-DNA tagging Use of T-DNA as insertional mutagen Random tagging Available populations of T-DNA transformants Promoter/enhancer trapping Analysis of T-DNA mutants and cloning a tagged gene Further applications of T-DNA tagging Map-based cloning in Arabidopsis / Locating the mutation of interest relative to DNA markers Determining an approximate map position Identifying a short genetic interval containing the mutation as a prelude to isolating the gene Placing the gene on the physical map Chromosome landing Chromosome walking with YAC clones Identification of the gene Location of the gene by molecular complementation Determining the structure of the gene Perspectives Physical mapping: YACs, BACs, cosmids, and nucleotide sequences / Genome mapping with YAC clones Genome mapping with BAC and P1 clones Communal resources Construction of BAC libraries Genome mapping with BACs High resolution mapping with cosmids Approaches to mapping with cosmids Construction of cosmid libraries Nucleotide sequences--the ultimate mapping tool EST sequencing project Genome sequencing project Sequence-based mapping Web-based bioinformatic tools for Arabidopsis researchers / What is bioinformatics Sources of Arabidopsis bioinformatic data Basic tools for the Internet Web basics Getting onto the Web Using your browser Browser tips and errors Privacy issues Scenarios of bioinformatic use in Arabidopsis research Gene information resources General gene information Arabidopsis gene information Plant gene information Genetic maps Physical maps Sequencing Arabidopsis genome initiative (AGI) Annotation of sequences by AGI Caveats in annotation Sequence contigs from AtDB Sequence analysis tools BLAST FASTA EST databases Gene identification programs Gene family analyses Motif analyses Protein structures Comprehensive sequence analysis tools Current issues and future directions in bioinformatics Some important bioinformatic issues Bioinformatic tools currently under development for Arabidopsis research. |
| title_auth | Arabidopsis : a practical approach / |
| title_exact_search | Arabidopsis : a practical approach / |
| title_full | Arabidopsis : a practical approach / edited by Zoe A. Wilson. |
| title_fullStr | Arabidopsis : a practical approach / edited by Zoe A. Wilson. |
| title_full_unstemmed | Arabidopsis : a practical approach / edited by Zoe A. Wilson. |
| title_short | Arabidopsis : |
| title_sort | arabidopsis a practical approach |
| title_sub | a practical approach / |
| topic | Arabidopsis Laboratory manuals. Arabidopsis Molecular aspects Laboratory manuals. Arabidopsis genetics https://id.nlm.nih.gov/mesh/D017360Q000235 Plants, Genetically Modified genetics https://id.nlm.nih.gov/mesh/D030821Q000235 Arabidopsis Manuels de laboratoire. Arabidopsis Aspect moléculaire Manuels de laboratoire. NATURE Plants General. bisacsh Arabidopsis fast Arabidopsis. fmesh |
| topic_facet | Arabidopsis Laboratory manuals. Arabidopsis Molecular aspects Laboratory manuals. Arabidopsis genetics Plants, Genetically Modified genetics Arabidopsis Manuels de laboratoire. Arabidopsis Aspect moléculaire Manuels de laboratoire. NATURE Plants General. Arabidopsis Arabidopsis. Laboratory Manual Laboratory manuals Laboratory manuals. Manuels de laboratoire. |
| url | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98516 |
| work_keys_str_mv | AT wilsonzoea arabidopsisapracticalapproach |