Signal transduction :: a practical approach /
Signal transduction is the name given to the processes that allow extracellular information to be converted into an intracellular message and in turn a physiological response. They are among the most highly studied processes in biology.
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Oxford ; New York :
Oxford University Press,
1999.
|
| Ausgabe: | 2nd ed. |
| Schriftenreihe: | Practical approach series ;
209. |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Zusammenfassung: | Signal transduction is the name given to the processes that allow extracellular information to be converted into an intracellular message and in turn a physiological response. They are among the most highly studied processes in biology. |
| Beschreibung: | 1 online resource (xxvii, 396 pages) : illustrations |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 0585484066 9780585484068 9780191565878 0191565873 1280375655 9781280375651 9786610375653 6610375658 |
Internformat
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| 245 | 0 | 0 | |a Signal transduction : |b a practical approach / |c edited by G. Milligan. |
| 250 | |a 2nd ed. | ||
| 260 | |a Oxford ; |a New York : |b Oxford University Press, |c 1999. | ||
| 300 | |a 1 online resource (xxvii, 396 pages) : |b illustrations | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 490 | 1 | |a The practical approach series ; |v 209 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |t Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy / |r Ulrik Gether and Brian Kobilka -- |t Probing the structure of receptor-binding sites by the substituted-cysteine accessibility method / |r Merrill M. Simpson, George Liapakis, and Jonathan A. Javitch -- |t Post-translational acylation of signal transducing polypeptides : palmitoylation of G protein-coupled receptors / |r Michel Bouvier [and others] -- |t G protein-coupled receptor phosphorylation and desensitization / |r Andrew B. Tobin, Angela Rae, and David C. Budd -- |t G proteins and their identification / |r Ian Mullaney -- |t Construction and analysis of receptor-G protein fusion proteins / |r Alan Wise -- |t Application of the baculoviral expression system to signal transduction / |r Andrew Paterson -- |t Reporter gene systems for the study of G protein-coupled receptor signal transduction in mammalian cells / |r Stephen Rees, Susan Brown and Jenny Stables -- |t Adenylyl cyclases and cyclic AMP / |r Maurice K.C. Ho and Yung H. Wong -- |t Analysis of the polyphosphorylated inositol lipos of Saccharomyces cerevisiae / |r Stephen K. Dove and Robert H. Michell -- |t Phosphoinositide 3-kinases / |r K.E. Anderson, L.R. Stephens, and P.T. Hawkins -- |t Phospholipase D and phosphatidylcholine metabolism / |r Kathryn E. Meier and Terra C. Gibbs -- |t Signal transduction by sphingosine kinase / |r Dagmar Meyer zu Heringdorf Chris J. Van Koppen and Karl H. Jakobs -- |t Detection, isolation, and quantitative assay of mitogen-activated protein kinases in intact cells and tissues / |r Neil G. Anderson -- |t Measuring inositol 1,4,5-trisphosphate-evoked ⁴⁵Ca² release from intracellular Ca² stores / |r Colin W. Taylor and Jonathan S. Marchant. |
| 588 | 0 | |a Print version record. | |
| 520 | |a Signal transduction is the name given to the processes that allow extracellular information to be converted into an intracellular message and in turn a physiological response. They are among the most highly studied processes in biology. | ||
| 546 | |a English. | ||
| 650 | 0 | |a G proteins |x Research |x Methodology. | |
| 650 | 0 | |a Cellular signal transduction |x Research |x Methodology. | |
| 650 | 0 | |a G proteins |x Receptors |x Research |x Methodology. | |
| 650 | 0 | |a G proteins. |0 http://id.loc.gov/authorities/subjects/sh89000191 | |
| 650 | 0 | |a Membrane proteins. |0 http://id.loc.gov/authorities/subjects/sh85083471 | |
| 650 | 1 | 2 | |a Signal Transduction |x physiology |
| 650 | 2 | 2 | |a Biochemistry |x methods |
| 650 | 2 | 2 | |a GTP-Binding Proteins |x metabolism |
| 650 | 2 | |a GTP-Binding Proteins |0 https://id.nlm.nih.gov/mesh/D019204 | |
| 650 | 2 | |a Membrane Proteins |0 https://id.nlm.nih.gov/mesh/D008565 | |
| 650 | 6 | |a Protéines G |x Recherche |x Méthodologie. | |
| 650 | 6 | |a Transduction du signal cellulaire |x Recherche |x Méthodologie. | |
| 650 | 6 | |a Protéines G |x Récepteurs |x Recherche |x Méthodologie. | |
| 650 | 6 | |a Protéines G. | |
| 650 | 6 | |a Protéines membranaires. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Cell Biology. |2 bisacsh | |
| 650 | 7 | |a Membrane proteins |2 fast | |
| 650 | 7 | |a G proteins |2 fast | |
| 650 | 7 | |a Cellular signal transduction |x Research |x Methodology |2 fast | |
| 650 | 7 | |a G proteins |x Research |x Methodology |2 fast | |
| 650 | 7 | |a Transduction du signal. |2 fmesh | |
| 650 | 7 | |a Sous-unites alpha Gs des proteines G. |2 fmesh | |
| 650 | 7 | |a Proteines G. |2 fmesh | |
| 650 | 7 | |a Biosignal |2 gnd |0 http://d-nb.info/gnd/4227585-4 | |
| 650 | 7 | |a Biosignalverarbeitung |2 gnd |0 http://d-nb.info/gnd/4006899-7 | |
| 650 | 7 | |a Plasmamembran |2 gnd |0 http://d-nb.info/gnd/4067550-6 | |
| 650 | 7 | |a Signalverarbeitung |2 gnd |0 http://d-nb.info/gnd/4054947-1 | |
| 650 | 7 | |a Proteines G |x Recepteurs |x Manuels de laboratoire. |2 ram | |
| 650 | 7 | |a Transduction du signal cellulaire |x Manuels de laboratoire. |2 ram | |
| 700 | 1 | |a Milligan, Graeme. | |
| 776 | 0 | 8 | |i Print version: |t Signal transduction. |b 2nd ed. |d Oxford ; New York : Oxford University Press, 1999 |z 0199637210 |z 0199637202 |w (DLC) 99026199 |w (OCoLC)41070920 |
| 830 | 0 | |a Practical approach series ; |v 209. |0 http://id.loc.gov/authorities/names/n84709852 | |
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| 880 | 8 | |6 505-00/(S |a 5. G proteins and their identification1. Introduction; 2. Production of crude plasma membrane fractions for analysis of G proteins; 3. Gel electrophoresis of G proteins; Mono-ADP-ribosylation of G proteins by bacterial exotoxins; Gel electrophoresis of G proteins; 4. Immunological methods; Immunization and serum collection; Immunoblotting and immunoprecipitation; 5. Quantification of G protein α subunits; 6. Functional aspects of G protein signalling; Determination of GTP hydrolysis in membrane preparations; Measurement of receptor-stimulated [[sup(35)]S]GTPγS binding in membrane preparations. | |
| 880 | 0 | |6 505-00/(S |a Intro; Contents; List of Contributors; Abbreviations; 1. Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy; 1. Introduction; 2. Expression and purification of the β2 adrenergic receptor; 3. Fluorescence labelling and spectroscopic analysis of the purified β2 adrenergic receptor; 4. Site-selective fluorescent labelling of the β2 adrenergic receptor; Labelling of cysteines; Site-specific labelling at lysines; 5. Concluding remarks; References. | |
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Datensatz im Suchindex
| DE-BY-FWS_katkey | ZDB-4-EBA-ocm53957620 |
|---|---|
| _version_ | 1816881613073547264 |
| adam_text | |
| any_adam_object | |
| author2 | Milligan, Graeme |
| author2_role | |
| author2_variant | g m gm |
| author_additional | Ulrik Gether and Brian Kobilka -- Merrill M. Simpson, George Liapakis, and Jonathan A. Javitch -- Michel Bouvier [and others] -- Andrew B. Tobin, Angela Rae, and David C. Budd -- Ian Mullaney -- Alan Wise -- Andrew Paterson -- Stephen Rees, Susan Brown and Jenny Stables -- Maurice K.C. Ho and Yung H. Wong -- Stephen K. Dove and Robert H. Michell -- K.E. Anderson, L.R. Stephens, and P.T. Hawkins -- Kathryn E. Meier and Terra C. Gibbs -- Dagmar Meyer zu Heringdorf Chris J. Van Koppen and Karl H. Jakobs -- Neil G. Anderson -- Colin W. Taylor and Jonathan S. Marchant. |
| author_facet | Milligan, Graeme |
| author_sort | Milligan, Graeme |
| building | Verbundindex |
| bvnumber | localFWS |
| callnumber-first | Q - Science |
| callnumber-label | QP552 |
| callnumber-raw | QP552.G16 S54 1999eb |
| callnumber-search | QP552.G16 S54 1999eb |
| callnumber-sort | QP 3552 G16 S54 41999EB |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 5100 WC 5150 WE 5320 |
| collection | ZDB-4-EBA |
| contents | Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy / Probing the structure of receptor-binding sites by the substituted-cysteine accessibility method / Post-translational acylation of signal transducing polypeptides : palmitoylation of G protein-coupled receptors / G protein-coupled receptor phosphorylation and desensitization / G proteins and their identification / Construction and analysis of receptor-G protein fusion proteins / Application of the baculoviral expression system to signal transduction / Reporter gene systems for the study of G protein-coupled receptor signal transduction in mammalian cells / Adenylyl cyclases and cyclic AMP / Analysis of the polyphosphorylated inositol lipos of Saccharomyces cerevisiae / Phosphoinositide 3-kinases / Phospholipase D and phosphatidylcholine metabolism / Signal transduction by sphingosine kinase / Detection, isolation, and quantitative assay of mitogen-activated protein kinases in intact cells and tissues / Measuring inositol 1,4,5-trisphosphate-evoked ⁴⁵Ca² release from intracellular Ca² stores / |
| ctrlnum | (OCoLC)53957620 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 2nd ed. |
| format | Electronic eBook |
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| id | ZDB-4-EBA-ocm53957620 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:15:30Z |
| institution | BVB |
| isbn | 0585484066 9780585484068 9780191565878 0191565873 1280375655 9781280375651 9786610375653 6610375658 |
| language | English |
| oclc_num | 53957620 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xxvii, 396 pages) : illustrations |
| psigel | ZDB-4-EBA |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Oxford University Press, |
| record_format | marc |
| series | Practical approach series ; |
| series2 | The practical approach series ; |
| spelling | Signal transduction : a practical approach / edited by G. Milligan. 2nd ed. Oxford ; New York : Oxford University Press, 1999. 1 online resource (xxvii, 396 pages) : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier The practical approach series ; 209 Includes bibliographical references and index. Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy / Ulrik Gether and Brian Kobilka -- Probing the structure of receptor-binding sites by the substituted-cysteine accessibility method / Merrill M. Simpson, George Liapakis, and Jonathan A. Javitch -- Post-translational acylation of signal transducing polypeptides : palmitoylation of G protein-coupled receptors / Michel Bouvier [and others] -- G protein-coupled receptor phosphorylation and desensitization / Andrew B. Tobin, Angela Rae, and David C. Budd -- G proteins and their identification / Ian Mullaney -- Construction and analysis of receptor-G protein fusion proteins / Alan Wise -- Application of the baculoviral expression system to signal transduction / Andrew Paterson -- Reporter gene systems for the study of G protein-coupled receptor signal transduction in mammalian cells / Stephen Rees, Susan Brown and Jenny Stables -- Adenylyl cyclases and cyclic AMP / Maurice K.C. Ho and Yung H. Wong -- Analysis of the polyphosphorylated inositol lipos of Saccharomyces cerevisiae / Stephen K. Dove and Robert H. Michell -- Phosphoinositide 3-kinases / K.E. Anderson, L.R. Stephens, and P.T. Hawkins -- Phospholipase D and phosphatidylcholine metabolism / Kathryn E. Meier and Terra C. Gibbs -- Signal transduction by sphingosine kinase / Dagmar Meyer zu Heringdorf Chris J. Van Koppen and Karl H. Jakobs -- Detection, isolation, and quantitative assay of mitogen-activated protein kinases in intact cells and tissues / Neil G. Anderson -- Measuring inositol 1,4,5-trisphosphate-evoked ⁴⁵Ca² release from intracellular Ca² stores / Colin W. Taylor and Jonathan S. Marchant. Print version record. Signal transduction is the name given to the processes that allow extracellular information to be converted into an intracellular message and in turn a physiological response. They are among the most highly studied processes in biology. English. G proteins Research Methodology. Cellular signal transduction Research Methodology. G proteins Receptors Research Methodology. G proteins. http://id.loc.gov/authorities/subjects/sh89000191 Membrane proteins. http://id.loc.gov/authorities/subjects/sh85083471 Signal Transduction physiology Biochemistry methods GTP-Binding Proteins metabolism GTP-Binding Proteins https://id.nlm.nih.gov/mesh/D019204 Membrane Proteins https://id.nlm.nih.gov/mesh/D008565 Protéines G Recherche Méthodologie. Transduction du signal cellulaire Recherche Méthodologie. Protéines G Récepteurs Recherche Méthodologie. Protéines G. Protéines membranaires. SCIENCE Life Sciences Cell Biology. bisacsh Membrane proteins fast G proteins fast Cellular signal transduction Research Methodology fast G proteins Research Methodology fast Transduction du signal. fmesh Sous-unites alpha Gs des proteines G. fmesh Proteines G. fmesh Biosignal gnd http://d-nb.info/gnd/4227585-4 Biosignalverarbeitung gnd http://d-nb.info/gnd/4006899-7 Plasmamembran gnd http://d-nb.info/gnd/4067550-6 Signalverarbeitung gnd http://d-nb.info/gnd/4054947-1 Proteines G Recepteurs Manuels de laboratoire. ram Transduction du signal cellulaire Manuels de laboratoire. ram Milligan, Graeme. Print version: Signal transduction. 2nd ed. Oxford ; New York : Oxford University Press, 1999 0199637210 0199637202 (DLC) 99026199 (OCoLC)41070920 Practical approach series ; 209. http://id.loc.gov/authorities/names/n84709852 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98488 Volltext 505-00/(S 5. G proteins and their identification1. Introduction; 2. Production of crude plasma membrane fractions for analysis of G proteins; 3. Gel electrophoresis of G proteins; Mono-ADP-ribosylation of G proteins by bacterial exotoxins; Gel electrophoresis of G proteins; 4. Immunological methods; Immunization and serum collection; Immunoblotting and immunoprecipitation; 5. Quantification of G protein α subunits; 6. Functional aspects of G protein signalling; Determination of GTP hydrolysis in membrane preparations; Measurement of receptor-stimulated [[sup(35)]S]GTPγS binding in membrane preparations. 505-00/(S Intro; Contents; List of Contributors; Abbreviations; 1. Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy; 1. Introduction; 2. Expression and purification of the β2 adrenergic receptor; 3. Fluorescence labelling and spectroscopic analysis of the purified β2 adrenergic receptor; 4. Site-selective fluorescent labelling of the β2 adrenergic receptor; Labelling of cysteines; Site-specific labelling at lysines; 5. Concluding remarks; References. |
| spellingShingle | Signal transduction : a practical approach / Practical approach series ; Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy / Probing the structure of receptor-binding sites by the substituted-cysteine accessibility method / Post-translational acylation of signal transducing polypeptides : palmitoylation of G protein-coupled receptors / G protein-coupled receptor phosphorylation and desensitization / G proteins and their identification / Construction and analysis of receptor-G protein fusion proteins / Application of the baculoviral expression system to signal transduction / Reporter gene systems for the study of G protein-coupled receptor signal transduction in mammalian cells / Adenylyl cyclases and cyclic AMP / Analysis of the polyphosphorylated inositol lipos of Saccharomyces cerevisiae / Phosphoinositide 3-kinases / Phospholipase D and phosphatidylcholine metabolism / Signal transduction by sphingosine kinase / Detection, isolation, and quantitative assay of mitogen-activated protein kinases in intact cells and tissues / Measuring inositol 1,4,5-trisphosphate-evoked ⁴⁵Ca² release from intracellular Ca² stores / G proteins Research Methodology. Cellular signal transduction Research Methodology. G proteins Receptors Research Methodology. G proteins. http://id.loc.gov/authorities/subjects/sh89000191 Membrane proteins. http://id.loc.gov/authorities/subjects/sh85083471 Signal Transduction physiology Biochemistry methods GTP-Binding Proteins metabolism GTP-Binding Proteins https://id.nlm.nih.gov/mesh/D019204 Membrane Proteins https://id.nlm.nih.gov/mesh/D008565 Protéines G Recherche Méthodologie. Transduction du signal cellulaire Recherche Méthodologie. Protéines G Récepteurs Recherche Méthodologie. Protéines G. Protéines membranaires. SCIENCE Life Sciences Cell Biology. bisacsh Membrane proteins fast G proteins fast Cellular signal transduction Research Methodology fast G proteins Research Methodology fast Transduction du signal. fmesh Sous-unites alpha Gs des proteines G. fmesh Proteines G. fmesh Biosignal gnd http://d-nb.info/gnd/4227585-4 Biosignalverarbeitung gnd http://d-nb.info/gnd/4006899-7 Plasmamembran gnd http://d-nb.info/gnd/4067550-6 Signalverarbeitung gnd http://d-nb.info/gnd/4054947-1 Proteines G Recepteurs Manuels de laboratoire. ram Transduction du signal cellulaire Manuels de laboratoire. ram |
| subject_GND | http://id.loc.gov/authorities/subjects/sh89000191 http://id.loc.gov/authorities/subjects/sh85083471 https://id.nlm.nih.gov/mesh/D019204 https://id.nlm.nih.gov/mesh/D008565 http://d-nb.info/gnd/4227585-4 http://d-nb.info/gnd/4006899-7 http://d-nb.info/gnd/4067550-6 http://d-nb.info/gnd/4054947-1 |
| title | Signal transduction : a practical approach / |
| title_alt | Direct assessment of conformational changes in G protein-coupled receptors, using fluorescence spectroscopy / Probing the structure of receptor-binding sites by the substituted-cysteine accessibility method / Post-translational acylation of signal transducing polypeptides : palmitoylation of G protein-coupled receptors / G protein-coupled receptor phosphorylation and desensitization / G proteins and their identification / Construction and analysis of receptor-G protein fusion proteins / Application of the baculoviral expression system to signal transduction / Reporter gene systems for the study of G protein-coupled receptor signal transduction in mammalian cells / Adenylyl cyclases and cyclic AMP / Analysis of the polyphosphorylated inositol lipos of Saccharomyces cerevisiae / Phosphoinositide 3-kinases / Phospholipase D and phosphatidylcholine metabolism / Signal transduction by sphingosine kinase / Detection, isolation, and quantitative assay of mitogen-activated protein kinases in intact cells and tissues / Measuring inositol 1,4,5-trisphosphate-evoked ⁴⁵Ca² release from intracellular Ca² stores / |
| title_auth | Signal transduction : a practical approach / |
| title_exact_search | Signal transduction : a practical approach / |
| title_full | Signal transduction : a practical approach / edited by G. Milligan. |
| title_fullStr | Signal transduction : a practical approach / edited by G. Milligan. |
| title_full_unstemmed | Signal transduction : a practical approach / edited by G. Milligan. |
| title_short | Signal transduction : |
| title_sort | signal transduction a practical approach |
| title_sub | a practical approach / |
| topic | G proteins Research Methodology. Cellular signal transduction Research Methodology. G proteins Receptors Research Methodology. G proteins. http://id.loc.gov/authorities/subjects/sh89000191 Membrane proteins. http://id.loc.gov/authorities/subjects/sh85083471 Signal Transduction physiology Biochemistry methods GTP-Binding Proteins metabolism GTP-Binding Proteins https://id.nlm.nih.gov/mesh/D019204 Membrane Proteins https://id.nlm.nih.gov/mesh/D008565 Protéines G Recherche Méthodologie. Transduction du signal cellulaire Recherche Méthodologie. Protéines G Récepteurs Recherche Méthodologie. Protéines G. Protéines membranaires. SCIENCE Life Sciences Cell Biology. bisacsh Membrane proteins fast G proteins fast Cellular signal transduction Research Methodology fast G proteins Research Methodology fast Transduction du signal. fmesh Sous-unites alpha Gs des proteines G. fmesh Proteines G. fmesh Biosignal gnd http://d-nb.info/gnd/4227585-4 Biosignalverarbeitung gnd http://d-nb.info/gnd/4006899-7 Plasmamembran gnd http://d-nb.info/gnd/4067550-6 Signalverarbeitung gnd http://d-nb.info/gnd/4054947-1 Proteines G Recepteurs Manuels de laboratoire. ram Transduction du signal cellulaire Manuels de laboratoire. ram |
| topic_facet | G proteins Research Methodology. Cellular signal transduction Research Methodology. G proteins Receptors Research Methodology. G proteins. Membrane proteins. Signal Transduction physiology Biochemistry methods GTP-Binding Proteins metabolism GTP-Binding Proteins Membrane Proteins Protéines G Recherche Méthodologie. Transduction du signal cellulaire Recherche Méthodologie. Protéines G Récepteurs Recherche Méthodologie. Protéines G. Protéines membranaires. SCIENCE Life Sciences Cell Biology. Membrane proteins G proteins Cellular signal transduction Research Methodology G proteins Research Methodology Transduction du signal. Sous-unites alpha Gs des proteines G. Proteines G. Biosignal Biosignalverarbeitung Plasmamembran Signalverarbeitung Proteines G Recepteurs Manuels de laboratoire. Transduction du signal cellulaire Manuels de laboratoire. |
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| work_keys_str_mv | AT milligangraeme signaltransductionapracticalapproach |