Post-translational processing :: a practical approach /
Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding a...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Oxford ; New York :
Oxford University Press,
1999.
|
| Schriftenreihe: | Practical approach series ;
203. |
| Schlagworte: | |
| Online-Zugang: | Volltext Volltext |
| Zusammenfassung: | Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing the is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of the best current methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approached. |
| Beschreibung: | 1 online resource (xx, 317 pages) : illustrations |
| Bibliographie: | Includes bibliographical references and index. |
| ISBN: | 0585483663 9780585483665 1280375760 9781280375767 9780191566035 0191566039 |
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| 504 | |a Includes bibliographical references and index. | ||
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| 520 | |a Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing the is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of the best current methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approached. | ||
| 505 | 0 | |a Intro; Contents; List of Contributors; Abbreviations; 1. Sequence analysis of expressed proteins; 1. Introduction; 2. N-terminal sequence analysis; Automated sequencing; 3. Sample preparation; Sample preparation by SDS-PAGE; Sample preparation by HPLC; Other procedures for sample preparation; 4. N-terminal blocking; Protein fragmentation; 5. C-terminal sequencing; Chemical analysis of the C-terminus; Enzymic analysis of the C-terminus; 6. Mass spectrometric analysis of proteins; A brief guide to mass spectrometers; Molecular mass determination; Sequencing by mass spectrometry; References | |
| 505 | 8 | |a 2. Protein folding and import into organelles1. Introduction; 2. Preparation of preprotein substrates for in vitro import into organelles; Preparation of mRNA for in vitro translation; Synthesis of preproteins in the reticulocyte lysate; Synthesis of preproteins in wheat germ lysate; Synthesis of preproteins in yeast cytosol; Synthesis of preproteins in Escherichia coli; Dihydrofolate reductase as a model protein for import studies; 3. Import of proteins into mitochondria; Isolation of mitochondria for import studies; Import of preproteins into isolated mitochondria | |
| 505 | 8 | |a Generation of translocation intermediatesLocalization of imported proteins; 4. Import of proteins into microsomes (endoplasmic reticulum); Isolation of microsomes; Import of preproteins into microsomes; 5. Import of proteins into other organelles; 6. Analysis of protein import into organelles; Monitoring the association of proteins with membranes; Analysis of protein complexes involved in organelle import; Analysis of protein folding after import into organelles; Acknowledgements; References; 3. Analysis of protein phosphorylation; 1. Introduction | |
| 505 | 8 | |a 2. Investigating protein phosphorylation systems3. Phosphorylation of proteins in intact preparations; General considerations; Intact animals; Tissue slices; Isolated cells; 4. Phosphorylation of proteins in cell-free preparations; General considerations; Labelling and stimulation of cell-free preparations; 5. Analysis of phosphorylated proteins; General considerations; Quantification of phosphoproteins; Phosphoprotein separation; Protein isolation by immunomethods; Analysis of multisite phosphorylation; Phosphoamino acid analysis; Analysis of the state of phosphorylation | |
| 505 | 8 | |a 6. Analysis of protein kinasesGeneral considerations; Analysis of protein kinase activity in vitro; Analysis of specific protein kinases in vitro; Analysis of protein kinase activity in intact cells; Purification of protein kinases; 7. Phosphoprotein phosphatases; General considerations; P-Ser/P-Thr phosphoprotein phosphatases; Tyrosine-specific phosphoprotein phosphatases; References; 4. Protein glycosylation; 1. Introduction; Protein glycosylation; Oligosaccharide structures; Glycosylation pathways; Characterization of protein glycosylation; 2. Is my protein glycosylated? | |
| 650 | 0 | |a Post-translational modification. |0 http://id.loc.gov/authorities/subjects/sh88005292 | |
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| 650 | 7 | |a Protéines |x Modifications posttraductionnelles. |2 ram | |
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| contents | Intro; Contents; List of Contributors; Abbreviations; 1. Sequence analysis of expressed proteins; 1. Introduction; 2. N-terminal sequence analysis; Automated sequencing; 3. Sample preparation; Sample preparation by SDS-PAGE; Sample preparation by HPLC; Other procedures for sample preparation; 4. N-terminal blocking; Protein fragmentation; 5. C-terminal sequencing; Chemical analysis of the C-terminus; Enzymic analysis of the C-terminus; 6. Mass spectrometric analysis of proteins; A brief guide to mass spectrometers; Molecular mass determination; Sequencing by mass spectrometry; References 2. Protein folding and import into organelles1. Introduction; 2. Preparation of preprotein substrates for in vitro import into organelles; Preparation of mRNA for in vitro translation; Synthesis of preproteins in the reticulocyte lysate; Synthesis of preproteins in wheat germ lysate; Synthesis of preproteins in yeast cytosol; Synthesis of preproteins in Escherichia coli; Dihydrofolate reductase as a model protein for import studies; 3. Import of proteins into mitochondria; Isolation of mitochondria for import studies; Import of preproteins into isolated mitochondria Generation of translocation intermediatesLocalization of imported proteins; 4. Import of proteins into microsomes (endoplasmic reticulum); Isolation of microsomes; Import of preproteins into microsomes; 5. Import of proteins into other organelles; 6. Analysis of protein import into organelles; Monitoring the association of proteins with membranes; Analysis of protein complexes involved in organelle import; Analysis of protein folding after import into organelles; Acknowledgements; References; 3. Analysis of protein phosphorylation; 1. Introduction 2. Investigating protein phosphorylation systems3. Phosphorylation of proteins in intact preparations; General considerations; Intact animals; Tissue slices; Isolated cells; 4. Phosphorylation of proteins in cell-free preparations; General considerations; Labelling and stimulation of cell-free preparations; 5. Analysis of phosphorylated proteins; General considerations; Quantification of phosphoproteins; Phosphoprotein separation; Protein isolation by immunomethods; Analysis of multisite phosphorylation; Phosphoamino acid analysis; Analysis of the state of phosphorylation 6. Analysis of protein kinasesGeneral considerations; Analysis of protein kinase activity in vitro; Analysis of specific protein kinases in vitro; Analysis of protein kinase activity in intact cells; Purification of protein kinases; 7. Phosphoprotein phosphatases; General considerations; P-Ser/P-Thr phosphoprotein phosphatases; Tyrosine-specific phosphoprotein phosphatases; References; 4. Protein glycosylation; 1. Introduction; Protein glycosylation; Oligosaccharide structures; Glycosylation pathways; Characterization of protein glycosylation; 2. Is my protein glycosylated? |
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| dewey-ones | 572 - Biochemistry |
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| dewey-search | 572/.6 |
| dewey-sort | 3572 16 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Electronic eBook |
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| id | ZDB-4-EBA-ocm53957505 |
| illustrated | Illustrated |
| indexdate | 2024-11-27T13:15:30Z |
| institution | BVB |
| isbn | 0585483663 9780585483665 1280375760 9781280375767 9780191566035 0191566039 |
| language | English |
| oclc_num | 53957505 |
| open_access_boolean | |
| owner | MAIN DE-863 DE-BY-FWS |
| owner_facet | MAIN DE-863 DE-BY-FWS |
| physical | 1 online resource (xx, 317 pages) : illustrations |
| psigel | ZDB-4-EBA |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Oxford University Press, |
| record_format | marc |
| series | Practical approach series ; |
| series2 | Practical approach series ; |
| spelling | Post-translational processing : a practical approach / edited by S.J. Higgins and B.D. Hames. Oxford ; New York : Oxford University Press, 1999. 1 online resource (xx, 317 pages) : illustrations text txt rdacontent computer c rdamedia online resource cr rdacarrier Practical approach series ; 203 Includes bibliographical references and index. Print version record. Post - Translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing the is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of the best current methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approached. Intro; Contents; List of Contributors; Abbreviations; 1. Sequence analysis of expressed proteins; 1. Introduction; 2. N-terminal sequence analysis; Automated sequencing; 3. Sample preparation; Sample preparation by SDS-PAGE; Sample preparation by HPLC; Other procedures for sample preparation; 4. N-terminal blocking; Protein fragmentation; 5. C-terminal sequencing; Chemical analysis of the C-terminus; Enzymic analysis of the C-terminus; 6. Mass spectrometric analysis of proteins; A brief guide to mass spectrometers; Molecular mass determination; Sequencing by mass spectrometry; References 2. Protein folding and import into organelles1. Introduction; 2. Preparation of preprotein substrates for in vitro import into organelles; Preparation of mRNA for in vitro translation; Synthesis of preproteins in the reticulocyte lysate; Synthesis of preproteins in wheat germ lysate; Synthesis of preproteins in yeast cytosol; Synthesis of preproteins in Escherichia coli; Dihydrofolate reductase as a model protein for import studies; 3. Import of proteins into mitochondria; Isolation of mitochondria for import studies; Import of preproteins into isolated mitochondria Generation of translocation intermediatesLocalization of imported proteins; 4. Import of proteins into microsomes (endoplasmic reticulum); Isolation of microsomes; Import of preproteins into microsomes; 5. Import of proteins into other organelles; 6. Analysis of protein import into organelles; Monitoring the association of proteins with membranes; Analysis of protein complexes involved in organelle import; Analysis of protein folding after import into organelles; Acknowledgements; References; 3. Analysis of protein phosphorylation; 1. Introduction 2. Investigating protein phosphorylation systems3. Phosphorylation of proteins in intact preparations; General considerations; Intact animals; Tissue slices; Isolated cells; 4. Phosphorylation of proteins in cell-free preparations; General considerations; Labelling and stimulation of cell-free preparations; 5. Analysis of phosphorylated proteins; General considerations; Quantification of phosphoproteins; Phosphoprotein separation; Protein isolation by immunomethods; Analysis of multisite phosphorylation; Phosphoamino acid analysis; Analysis of the state of phosphorylation 6. Analysis of protein kinasesGeneral considerations; Analysis of protein kinase activity in vitro; Analysis of specific protein kinases in vitro; Analysis of protein kinase activity in intact cells; Purification of protein kinases; 7. Phosphoprotein phosphatases; General considerations; P-Ser/P-Thr phosphoprotein phosphatases; Tyrosine-specific phosphoprotein phosphatases; References; 4. Protein glycosylation; 1. Introduction; Protein glycosylation; Oligosaccharide structures; Glycosylation pathways; Characterization of protein glycosylation; 2. Is my protein glycosylated? Post-translational modification. http://id.loc.gov/authorities/subjects/sh88005292 Protein Biosynthesis Peptide Biosynthesis Protéines Modification post-traductionnelle. SCIENCE Life Sciences Biochemistry. bisacsh Post-translational modification fast Protéines Modifications posttraductionnelles. ram Higgins, S. J. (Steve J.) https://id.oclc.org/worldcat/entity/E39PBJmpcymDJ4XM6BtVWHQg8C http://id.loc.gov/authorities/names/n85350807 Hames, B. D. has work: Post-translational processing (Text) https://id.oclc.org/worldcat/entity/E39PCG9J4gqWRtxgfvGGRT6BT3 https://id.oclc.org/worldcat/ontology/hasWork Print version: Post-translational processing. Oxford ; New York : Oxford University Press, 1999 0199637946 0199637954 (DLC) 98052159 (OCoLC)40489139 Practical approach series ; 203. http://id.loc.gov/authorities/names/n84709852 FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=1943838 Volltext FWS01 ZDB-4-EBA FWS_PDA_EBA https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98471 Volltext |
| spellingShingle | Post-translational processing : a practical approach / Practical approach series ; Intro; Contents; List of Contributors; Abbreviations; 1. Sequence analysis of expressed proteins; 1. Introduction; 2. N-terminal sequence analysis; Automated sequencing; 3. Sample preparation; Sample preparation by SDS-PAGE; Sample preparation by HPLC; Other procedures for sample preparation; 4. N-terminal blocking; Protein fragmentation; 5. C-terminal sequencing; Chemical analysis of the C-terminus; Enzymic analysis of the C-terminus; 6. Mass spectrometric analysis of proteins; A brief guide to mass spectrometers; Molecular mass determination; Sequencing by mass spectrometry; References 2. Protein folding and import into organelles1. Introduction; 2. Preparation of preprotein substrates for in vitro import into organelles; Preparation of mRNA for in vitro translation; Synthesis of preproteins in the reticulocyte lysate; Synthesis of preproteins in wheat germ lysate; Synthesis of preproteins in yeast cytosol; Synthesis of preproteins in Escherichia coli; Dihydrofolate reductase as a model protein for import studies; 3. Import of proteins into mitochondria; Isolation of mitochondria for import studies; Import of preproteins into isolated mitochondria Generation of translocation intermediatesLocalization of imported proteins; 4. Import of proteins into microsomes (endoplasmic reticulum); Isolation of microsomes; Import of preproteins into microsomes; 5. Import of proteins into other organelles; 6. Analysis of protein import into organelles; Monitoring the association of proteins with membranes; Analysis of protein complexes involved in organelle import; Analysis of protein folding after import into organelles; Acknowledgements; References; 3. Analysis of protein phosphorylation; 1. Introduction 2. Investigating protein phosphorylation systems3. Phosphorylation of proteins in intact preparations; General considerations; Intact animals; Tissue slices; Isolated cells; 4. Phosphorylation of proteins in cell-free preparations; General considerations; Labelling and stimulation of cell-free preparations; 5. Analysis of phosphorylated proteins; General considerations; Quantification of phosphoproteins; Phosphoprotein separation; Protein isolation by immunomethods; Analysis of multisite phosphorylation; Phosphoamino acid analysis; Analysis of the state of phosphorylation 6. Analysis of protein kinasesGeneral considerations; Analysis of protein kinase activity in vitro; Analysis of specific protein kinases in vitro; Analysis of protein kinase activity in intact cells; Purification of protein kinases; 7. Phosphoprotein phosphatases; General considerations; P-Ser/P-Thr phosphoprotein phosphatases; Tyrosine-specific phosphoprotein phosphatases; References; 4. Protein glycosylation; 1. Introduction; Protein glycosylation; Oligosaccharide structures; Glycosylation pathways; Characterization of protein glycosylation; 2. Is my protein glycosylated? Post-translational modification. http://id.loc.gov/authorities/subjects/sh88005292 Protein Biosynthesis Peptide Biosynthesis Protéines Modification post-traductionnelle. SCIENCE Life Sciences Biochemistry. bisacsh Post-translational modification fast Protéines Modifications posttraductionnelles. ram |
| subject_GND | http://id.loc.gov/authorities/subjects/sh88005292 |
| title | Post-translational processing : a practical approach / |
| title_auth | Post-translational processing : a practical approach / |
| title_exact_search | Post-translational processing : a practical approach / |
| title_full | Post-translational processing : a practical approach / edited by S.J. Higgins and B.D. Hames. |
| title_fullStr | Post-translational processing : a practical approach / edited by S.J. Higgins and B.D. Hames. |
| title_full_unstemmed | Post-translational processing : a practical approach / edited by S.J. Higgins and B.D. Hames. |
| title_short | Post-translational processing : |
| title_sort | post translational processing a practical approach |
| title_sub | a practical approach / |
| topic | Post-translational modification. http://id.loc.gov/authorities/subjects/sh88005292 Protein Biosynthesis Peptide Biosynthesis Protéines Modification post-traductionnelle. SCIENCE Life Sciences Biochemistry. bisacsh Post-translational modification fast Protéines Modifications posttraductionnelles. ram |
| topic_facet | Post-translational modification. Protein Biosynthesis Peptide Biosynthesis Protéines Modification post-traductionnelle. SCIENCE Life Sciences Biochemistry. Post-translational modification Protéines Modifications posttraductionnelles. |
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