The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness:
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| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Aachen
[2023]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 79 Seiten Illustrationen, Diagramme 21 cm |
Internformat
MARC
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| 100 | 1 | |a Richter, Isabel |e Verfasser |0 (DE-588)1305711025 |4 aut | |
| 245 | 1 | 0 | |a The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| 264 | 1 | |a Aachen |c [2023] | |
| 300 | |a 79 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Rheinisch-Westfälische Technische Hochschule Aachen |d 2023 | ||
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Datensatz im Suchindex
| _version_ | 1822491028116996097 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
INTRODUCTION
.
1
AIM
.
11
MATERIALS
AND
METHODS
.
12
ANIMALS
.
15
TISSUE
COLLECTION
.
15
ISOLATION
OF
INTESTINAL
EPITHELIAL
CELLS
.
16
RNA
EXTRACTION
.
17
COMPLEMENTARY
DNA
(CDNA)
SYNTHESIS
.
17
QUANTITATIVE
PCR
(TAQMAN)
.
18
IMMUNOFLUORESCENCE
STAINING
.
19
LIST
OF
STAININGS
USED
.
21
FLUORESCENCE
MICROSCOPY
.
23
IMAGE
ANALYSIS
.
24
PREPARATION
OF
CRYOSECTIONS
.
26
LASER
CAPTURE
MICRODISSECTION
.
27
RNA
SEQUENCING
.
28
STATISTICAL
ANALYSIS
.
28
RESULTS
.
29
GENE
EXPRESSION
ANALYSIS
OF
NEONATE
SMALL
INTESTINAL
EPITHELIUM
.
29
PANETH
CELL
MARKERS
.
29
FACTORS
INVOLVED
IN
SECRETORY
LINEAGE
DIFFERENTIATION
.
30
INFLUENCE
OF
THE
MICROBIOTA
.
31
PROTEIN
EXPRESSION
ANALYSIS
IN
THE
SMALL
INTESTINE
OF
NEONATE
MICE
.
33
QUANTIFICATION
OF
CELLS
OF
THE
SECRETORY
LINEAGE
.
33
ESTABLISHMENT
OF
A
PROTOCOL
TO
ISOLATE
RNA
FROM
LASER
MICRODISSECTED
SMALL
INTESTINAL
FROZEN
TISSUE
SECTIONS
.
42
AGE
KINETIC
OF
RNA
EXPRESSION
IN
VILLUS
VS.
CRYPT/INTERVILLUS
AREA
.
45
TOP
UPREGULATED
GENES
IN
EPITHELIAL
CRYPTS/
INTERVILLUS
REGION
TISSUE
.
46
TOP
UPREGULATED
GENES
IN
EPITHELIAL
VILLUS
TISSUE
.
47
TOP
UPREGULATED
GENES
IN
CRYPT/INTERVILLUS
AND
VILLUS
TISSUE
ON
DAY
25
.
48
TOP
UPREGULATED
GENES
IN
CRYPT/INTERVILLUS
AND
VILLUS
TISSUE
ON
DAY
1
.
49
EXPRESSION
OF
TARGET
GENES
IN
LCM
TISSUE
SAMPLES
.
50
DISCUSSION
.
54
SUMMARY
.
63
ZUSAMMENFASSUNG
.
64
REFERENCES
.
65
DANKSAGUNG
.
77
ERKLARUNG
§
5
ABS.
1
ZUR
DATENAUFBEWAHRUNG
.
78
EIDESSTATTLICHE
ERKLARUNG
GEMAB
§
5
ABS.
(1)
UND
§
11
ABS.
(3)
12.
DER
PROMOTIONSORDNUNG
.
79 |
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| author | Richter, Isabel |
| author_GND | (DE-588)1305711025 |
| author_facet | Richter, Isabel |
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| author_sort | Richter, Isabel |
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| building | Verbundindex |
| bvnumber | BV050140313 |
| ctrlnum | (DE-599)DNB1312970030 |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2025-01-28T11:14:45Z |
| institution | BVB |
| language | English |
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| owner_facet | DE-355 DE-BY-UBR |
| physical | 79 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2023 |
| publishDateSearch | 2023 |
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| spelling | Richter, Isabel Verfasser (DE-588)1305711025 aut The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness Aachen [2023] 79 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2023 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1312970030/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035476803&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20240305 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Richter, Isabel The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| subject_GND | (DE-588)4113937-9 |
| title | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_auth | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_exact_search | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_full | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_fullStr | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_full_unstemmed | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_short | The influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| title_sort | the influence of microbial exposure on postnatal enterocyte differentiation and immune responsiveness |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1312970030/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035476803&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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