High Throughput Gene Screening: Methods and Protocols
This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologie...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana
2024
|
| Ausgabe: | 2025 |
| Schriftenreihe: | Methods in Molecular Biology
2866 |
| Schlagworte: | |
| Zusammenfassung: | This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology |
| Beschreibung: | Approx. 320 p. 41 illus., 39 illus. in color. - This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology The Evolution of Next-Generation Sequencing Technologies.- A Step-by-Step Guide to Sequencing and Assembly of Complete Bacterial Genomes using the Oxford Nanopore MinION.- Targeted Next-Generation Sequencing in Rare Diseases.- Collection and Processing of Samples for Next-Generation Sequencing to Study the Gut Microbiome.- Whole Genome Methylation Sequencing via Enzymatic Conversion (EM-seq): Protocol, Data Processing, and Analysis.- MNase-seq to Identify Genome-Wide DNA-Protein Interactions.- ATAC-seq for Characterizing Host and Pathogen Genome Accessibility during Virus Infection.- RNA Sequencing Protocols for Short-Read Sequencing.- Dissection of Gene Expression at the Single Cell Level: scRNA-seq.- The Study of Small RNA Sequencing from Biological Samples (miRNA-seq).- Biological Insights from RNA-RNA Interactomes in Bacteria, as Revealed by RIL-seq.- Extraction, Purification, and Next-Generation Sequencing (NGS) Analysis of DNA and RNA from Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue.- Using Chromosome Conformation Capture Combined with Deep Sequencing (Hi-C) to Study Genome Organization in Bacteria.- An Optimized Adaptation of DamID for NGS Applications.- How Have Massively Parallel Sequencing Technologies Furthered Our Understanding of Oncogenesis and Cancer Progression?.- The Use of Next-Generation Sequencing in Personalized Medicine. |
| Beschreibung: | 322 Seiten 254 mm |
| ISBN: | 9781071641910 |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV050123936 | ||
| 003 | DE-604 | ||
| 007 | t| | ||
| 008 | 250115s2024 xx |||| 00||| eng d | ||
| 020 | |a 9781071641910 |9 978-1-0716-4191-0 | ||
| 024 | 3 | |a 9781071641910 | |
| 035 | |a (DE-599)BVBBV050123936 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-29T | ||
| 100 | 1 | |a Carabetta, Valerie J. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a High Throughput Gene Screening |b Methods and Protocols |
| 250 | |a 2025 | ||
| 264 | 1 | |a New York, NY |b Humana |c 2024 | |
| 300 | |a 322 Seiten |c 254 mm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology |v 2866 | |
| 500 | |a Approx. 320 p. 41 illus., 39 illus. in color. - This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology | ||
| 500 | |a The Evolution of Next-Generation Sequencing Technologies.- A Step-by-Step Guide to Sequencing and Assembly of Complete Bacterial Genomes using the Oxford Nanopore MinION.- Targeted Next-Generation Sequencing in Rare Diseases.- Collection and Processing of Samples for Next-Generation Sequencing to Study the Gut Microbiome.- Whole Genome Methylation Sequencing via Enzymatic Conversion (EM-seq): Protocol, Data Processing, and Analysis.- MNase-seq to Identify Genome-Wide DNA-Protein Interactions.- ATAC-seq for Characterizing Host and Pathogen Genome Accessibility during Virus Infection.- RNA Sequencing Protocols for Short-Read Sequencing.- Dissection of Gene Expression at the Single Cell Level: scRNA-seq.- The Study of Small RNA Sequencing from Biological Samples (miRNA-seq).- Biological Insights from RNA-RNA Interactomes in Bacteria, as Revealed by RIL-seq.- Extraction, Purification, and Next-Generation Sequencing (NGS) Analysis of DNA and RNA from Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue.- Using Chromosome Conformation Capture Combined with Deep Sequencing (Hi-C) to Study Genome Organization in Bacteria.- An Optimized Adaptation of DamID for NGS Applications.- How Have Massively Parallel Sequencing Technologies Furthered Our Understanding of Oncogenesis and Cancer Progression?.- The Use of Next-Generation Sequencing in Personalized Medicine. | ||
| 520 | |a This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology | ||
| 650 | 4 | |a bicssc | |
| 650 | 4 | |a bisacsh | |
| 650 | 4 | |a Genetics | |
| 650 | 4 | |a Genetics | |
| 653 | |a Hardcover, Softcover / Biologie/Genetik, Gentechnik | ||
| 700 | 1 | |a Akintunde, Olaitan |e Sonstige |4 oth | |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-035460781 | |
Datensatz im Suchindex
| _version_ | 1821357649660215297 |
|---|---|
| adam_text | |
| any_adam_object | |
| author | Carabetta, Valerie J. |
| author_facet | Carabetta, Valerie J. |
| author_role | aut |
| author_sort | Carabetta, Valerie J. |
| author_variant | v j c vj vjc |
| building | Verbundindex |
| bvnumber | BV050123936 |
| ctrlnum | (DE-599)BVBBV050123936 |
| edition | 2025 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV050123936</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">250115s2024 xx |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781071641910</subfield><subfield code="9">978-1-0716-4191-0</subfield></datafield><datafield tag="024" ind1="3" ind2=" "><subfield code="a">9781071641910</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV050123936</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29T</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Carabetta, Valerie J.</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">High Throughput Gene Screening</subfield><subfield code="b">Methods and Protocols</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">2025</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Humana</subfield><subfield code="c">2024</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">322 Seiten</subfield><subfield code="c">254 mm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield><subfield code="v">2866</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Approx. 320 p. 41 illus., 39 illus. in color. - This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">The Evolution of Next-Generation Sequencing Technologies.- A Step-by-Step Guide to Sequencing and Assembly of Complete Bacterial Genomes using the Oxford Nanopore MinION.- Targeted Next-Generation Sequencing in Rare Diseases.- Collection and Processing of Samples for Next-Generation Sequencing to Study the Gut Microbiome.- Whole Genome Methylation Sequencing via Enzymatic Conversion (EM-seq): Protocol, Data Processing, and Analysis.- MNase-seq to Identify Genome-Wide DNA-Protein Interactions.- ATAC-seq for Characterizing Host and Pathogen Genome Accessibility during Virus Infection.- RNA Sequencing Protocols for Short-Read Sequencing.- Dissection of Gene Expression at the Single Cell Level: scRNA-seq.- The Study of Small RNA Sequencing from Biological Samples (miRNA-seq).- Biological Insights from RNA-RNA Interactomes in Bacteria, as Revealed by RIL-seq.- Extraction, Purification, and Next-Generation Sequencing (NGS) Analysis of DNA and RNA from Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue.- Using Chromosome Conformation Capture Combined with Deep Sequencing (Hi-C) to Study Genome Organization in Bacteria.- An Optimized Adaptation of DamID for NGS Applications.- How Have Massively Parallel Sequencing Technologies Furthered Our Understanding of Oncogenesis and Cancer Progression?.- The Use of Next-Generation Sequencing in Personalized Medicine.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bicssc</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetics</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Hardcover, Softcover / Biologie/Genetik, Gentechnik</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Akintunde, Olaitan</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-035460781</subfield></datafield></record></collection> |
| id | DE-604.BV050123936 |
| illustrated | Not Illustrated |
| indexdate | 2025-01-15T23:00:11Z |
| institution | BVB |
| isbn | 9781071641910 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-035460781 |
| open_access_boolean | |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | 322 Seiten 254 mm |
| publishDate | 2024 |
| publishDateSearch | 2024 |
| publishDateSort | 2024 |
| publisher | Humana |
| record_format | marc |
| series2 | Methods in Molecular Biology |
| spelling | Carabetta, Valerie J. Verfasser aut High Throughput Gene Screening Methods and Protocols 2025 New York, NY Humana 2024 322 Seiten 254 mm txt rdacontent n rdamedia nc rdacarrier Methods in Molecular Biology 2866 Approx. 320 p. 41 illus., 39 illus. in color. - This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology The Evolution of Next-Generation Sequencing Technologies.- A Step-by-Step Guide to Sequencing and Assembly of Complete Bacterial Genomes using the Oxford Nanopore MinION.- Targeted Next-Generation Sequencing in Rare Diseases.- Collection and Processing of Samples for Next-Generation Sequencing to Study the Gut Microbiome.- Whole Genome Methylation Sequencing via Enzymatic Conversion (EM-seq): Protocol, Data Processing, and Analysis.- MNase-seq to Identify Genome-Wide DNA-Protein Interactions.- ATAC-seq for Characterizing Host and Pathogen Genome Accessibility during Virus Infection.- RNA Sequencing Protocols for Short-Read Sequencing.- Dissection of Gene Expression at the Single Cell Level: scRNA-seq.- The Study of Small RNA Sequencing from Biological Samples (miRNA-seq).- Biological Insights from RNA-RNA Interactomes in Bacteria, as Revealed by RIL-seq.- Extraction, Purification, and Next-Generation Sequencing (NGS) Analysis of DNA and RNA from Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue.- Using Chromosome Conformation Capture Combined with Deep Sequencing (Hi-C) to Study Genome Organization in Bacteria.- An Optimized Adaptation of DamID for NGS Applications.- How Have Massively Parallel Sequencing Technologies Furthered Our Understanding of Oncogenesis and Cancer Progression?.- The Use of Next-Generation Sequencing in Personalized Medicine. This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology bicssc bisacsh Genetics Hardcover, Softcover / Biologie/Genetik, Gentechnik Akintunde, Olaitan Sonstige oth |
| spellingShingle | Carabetta, Valerie J. High Throughput Gene Screening Methods and Protocols bicssc bisacsh Genetics |
| title | High Throughput Gene Screening Methods and Protocols |
| title_auth | High Throughput Gene Screening Methods and Protocols |
| title_exact_search | High Throughput Gene Screening Methods and Protocols |
| title_full | High Throughput Gene Screening Methods and Protocols |
| title_fullStr | High Throughput Gene Screening Methods and Protocols |
| title_full_unstemmed | High Throughput Gene Screening Methods and Protocols |
| title_short | High Throughput Gene Screening |
| title_sort | high throughput gene screening methods and protocols |
| title_sub | Methods and Protocols |
| topic | bicssc bisacsh Genetics |
| topic_facet | bicssc bisacsh Genetics |
| work_keys_str_mv | AT carabettavaleriej highthroughputgenescreeningmethodsandprotocols AT akintundeolaitan highthroughputgenescreeningmethodsandprotocols |