Techniques in light microscopy:
"Transmitted techniques are the primary methods taught for well over 100 years, but include modern updates when applicable. The techniques roughly follow historical developments, starting with Brightfield and Darkfield microscopy and progressing through Polarized Light, Phase Contrast, Differen...
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Oxford
Oxford University Press
[2024]
|
| Zusammenfassung: | "Transmitted techniques are the primary methods taught for well over 100 years, but include modern updates when applicable. The techniques roughly follow historical developments, starting with Brightfield and Darkfield microscopy and progressing through Polarized Light, Phase Contrast, Differential Interference Contrast, and Modulation Contrast microscopy. The second section is concerned with the more modern techniques of epifluorescence microscopy. This section follows a progression from a theoretical description of fluorescence, through widefield epifluorescence, confocal, two photon, and selective plane imaging microscopy. Following that, there are two chapters that can be described as computational epifluorescence microscopy. That is, microscopy that has transitioned from strictly visual, to one that requires computational intervention to achieve diffraction-limited microscopy (restoration microscopy), or better (super resolution microscopy). The final chapter in this section describes super resolution epifluorescence microscopy, or resolution that is better than theoretical when using the Abbe or Rayleigh criteria. At the present time these techniques are in flux, with variations being invented nearly every year. This chapter describes the four fundamental techniques for attaining super-resolution. Included is a chapter on theoretical aspects of microscope resolution as well as one on the history of the microscope"-- |
| Beschreibung: | x, 244 Seiten |
| ISBN: | 9780198885832 |
Internformat
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| 100 | 1 | |a Ruzin, Steven E. |d 1951- |e Verfasser |0 (DE-588)173352995 |4 aut | |
| 245 | 1 | 0 | |a Techniques in light microscopy |c Steven E. Ruzin (University of California, Berkeley) |
| 264 | 1 | |a Oxford |b Oxford University Press |c [2024] | |
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| 520 | 3 | |a "Transmitted techniques are the primary methods taught for well over 100 years, but include modern updates when applicable. The techniques roughly follow historical developments, starting with Brightfield and Darkfield microscopy and progressing through Polarized Light, Phase Contrast, Differential Interference Contrast, and Modulation Contrast microscopy. The second section is concerned with the more modern techniques of epifluorescence microscopy. This section follows a progression from a theoretical description of fluorescence, through widefield epifluorescence, confocal, two photon, and selective plane imaging microscopy. Following that, there are two chapters that can be described as computational epifluorescence microscopy. That is, microscopy that has transitioned from strictly visual, to one that requires computational intervention to achieve diffraction-limited microscopy (restoration microscopy), or better (super resolution microscopy). The final chapter in this section describes super resolution epifluorescence microscopy, or resolution that is better than theoretical when using the Abbe or Rayleigh criteria. At the present time these techniques are in flux, with variations being invented nearly every year. This chapter describes the four fundamental techniques for attaining super-resolution. Included is a chapter on theoretical aspects of microscope resolution as well as one on the history of the microscope"-- | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 978-0-19-199413-5 |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-035235155 | |
Datensatz im Suchindex
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| author | Ruzin, Steven E. 1951- |
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| bvnumber | BV049896103 |
| ctrlnum | (OCoLC)1467261068 (DE-599)KXP1903785774 |
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| id | DE-604.BV049896103 |
| illustrated | Not Illustrated |
| indexdate | 2025-01-28T09:05:38Z |
| institution | BVB |
| isbn | 9780198885832 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-035235155 |
| oclc_num | 1467261068 |
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| owner | DE-20 |
| owner_facet | DE-20 |
| physical | x, 244 Seiten |
| publishDate | 2024 |
| publishDateSearch | 2024 |
| publishDateSort | 2024 |
| publisher | Oxford University Press |
| record_format | marc |
| spelling | Ruzin, Steven E. 1951- Verfasser (DE-588)173352995 aut Techniques in light microscopy Steven E. Ruzin (University of California, Berkeley) Oxford Oxford University Press [2024] x, 244 Seiten txt rdacontent n rdamedia nc rdacarrier "Transmitted techniques are the primary methods taught for well over 100 years, but include modern updates when applicable. The techniques roughly follow historical developments, starting with Brightfield and Darkfield microscopy and progressing through Polarized Light, Phase Contrast, Differential Interference Contrast, and Modulation Contrast microscopy. The second section is concerned with the more modern techniques of epifluorescence microscopy. This section follows a progression from a theoretical description of fluorescence, through widefield epifluorescence, confocal, two photon, and selective plane imaging microscopy. Following that, there are two chapters that can be described as computational epifluorescence microscopy. That is, microscopy that has transitioned from strictly visual, to one that requires computational intervention to achieve diffraction-limited microscopy (restoration microscopy), or better (super resolution microscopy). The final chapter in this section describes super resolution epifluorescence microscopy, or resolution that is better than theoretical when using the Abbe or Rayleigh criteria. At the present time these techniques are in flux, with variations being invented nearly every year. This chapter describes the four fundamental techniques for attaining super-resolution. Included is a chapter on theoretical aspects of microscope resolution as well as one on the history of the microscope"-- Erscheint auch als Online-Ausgabe 978-0-19-199413-5 |
| spellingShingle | Ruzin, Steven E. 1951- Techniques in light microscopy |
| title | Techniques in light microscopy |
| title_auth | Techniques in light microscopy |
| title_exact_search | Techniques in light microscopy |
| title_full | Techniques in light microscopy Steven E. Ruzin (University of California, Berkeley) |
| title_fullStr | Techniques in light microscopy Steven E. Ruzin (University of California, Berkeley) |
| title_full_unstemmed | Techniques in light microscopy Steven E. Ruzin (University of California, Berkeley) |
| title_short | Techniques in light microscopy |
| title_sort | techniques in light microscopy |
| work_keys_str_mv | AT ruzinstevene techniquesinlightmicroscopy |