Memory B-cells: methods and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2024
|
| Schriftenreihe: | Methods in molecular biology
2826 |
| Schlagworte: | |
| Beschreibung: | With online files/update. - This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Memory B-Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field Using ex vivo tonsil organoids to study memory B cells.- Ultrasound Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to assess B cell responses to Vaccination.- Human B cell receptor repertoire sequencing.- Visualising epigenetic modifications and nuclear bodies by immunofluorescence staining in naïve, activated and memory B cell subsets.- Measuring B cell Chromatin Accessibility by One-Step ATAC-seq.- Epigenomic profiling of B cell subsets by CUT&Tag.- In vivo CRISPR/Cas9-mediated gene ablation in murine B cells.- Isolation of rare antigen-specific memory B cells via antigen tetramers.- Detection of lymphocytic choriomeningitis virus-specific memory B cells using antigen tetramers.- B cell receptor transgenic mice as tools to study memory B cells.- ELISpots for detecting antigen-specific memory B cells in Infection or Autoimmunity.- Intracellular flow cytometry ("phosphoflow") to assess signal transduction in rare populations such as memory B cell subsets and plasma cells.- Quantifying human naïve B cell proliferation kinetics and differentiation in controlled in vitro cell culture.- Flow cytometric identification of human IgE+ B lineage subsets.- Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencing.-Assessing the survival of human plasma cells ex vivo.- Conversion of mouse-derived hybridomas to Tasmanian devil recombinant IgG antibodies. |
| Beschreibung: | xi, 252 Seiten Illustrationen, Diagramme 254 mm |
| ISBN: | 9781071639498 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV049868723 | ||
| 003 | DE-604 | ||
| 005 | 20241016 | ||
| 007 | t| | ||
| 008 | 240916s2024 xx a||| |||| 00||| eng d | ||
| 020 | |a 9781071639498 |9 978-1-0716-3949-8 | ||
| 024 | 3 | |a 9781071639498 | |
| 035 | |a (OCoLC)1455417545 | ||
| 035 | |a (DE-599)BVBBV049868723 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-29T | ||
| 245 | 1 | 0 | |a Memory B-cells |b methods and protocols |c edited by Kim L. Good-Jacobson |
| 264 | 1 | |a New York, NY |b Humana Press |c [2024 | |
| 300 | |a xi, 252 Seiten |b Illustrationen, Diagramme |c 254 mm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 2826 | |
| 500 | |a With online files/update. - This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Memory B-Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field | ||
| 500 | |a Using ex vivo tonsil organoids to study memory B cells.- Ultrasound Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to assess B cell responses to Vaccination.- Human B cell receptor repertoire sequencing.- Visualising epigenetic modifications and nuclear bodies by immunofluorescence staining in naïve, activated and memory B cell subsets.- Measuring B cell Chromatin Accessibility by One-Step ATAC-seq.- Epigenomic profiling of B cell subsets by CUT&Tag.- In vivo CRISPR/Cas9-mediated gene ablation in murine B cells.- Isolation of rare antigen-specific memory B cells via antigen tetramers.- Detection of lymphocytic choriomeningitis virus-specific memory B cells using antigen tetramers.- B cell receptor transgenic mice as tools to study memory B cells.- ELISpots for detecting antigen-specific memory B cells in Infection or Autoimmunity.- Intracellular flow cytometry ("phosphoflow") to assess signal transduction in rare populations such as memory B cell subsets and plasma cells.- Quantifying human naïve B cell proliferation kinetics and differentiation in controlled in vitro cell culture.- Flow cytometric identification of human IgE+ B lineage subsets.- Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencing.-Assessing the survival of human plasma cells ex vivo.- Conversion of mouse-derived hybridomas to Tasmanian devil recombinant IgG antibodies. | ||
| 650 | 4 | |a bicssc | |
| 650 | 4 | |a bicssc | |
| 650 | 4 | |a bisacsh | |
| 650 | 4 | |a bisacsh | |
| 650 | 4 | |a Experimental immunology | |
| 650 | 4 | |a Vaccines—Biotechnology | |
| 650 | 4 | |a Biomaterials | |
| 650 | 4 | |a Biology—Technique | |
| 653 | |a Hardcover, Softcover / Biologie/Sonstiges | ||
| 700 | 1 | |a Good-Jacobson, Kim L. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 978-1-0716-3950-4 |
| 830 | 0 | |a Methods in molecular biology |v 2826 |w (DE-604)BV035362695 |9 2826 | |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-035208248 | |
Datensatz im Suchindex
| _version_ | 1817696673808252928 |
|---|---|
| adam_text | |
| any_adam_object | |
| author2 | Good-Jacobson, Kim L. |
| author2_role | edt |
| author2_variant | k l g j klg klgj |
| author_facet | Good-Jacobson, Kim L. |
| building | Verbundindex |
| bvnumber | BV049868723 |
| ctrlnum | (OCoLC)1455417545 (DE-599)BVBBV049868723 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 cb4500</leader><controlfield tag="001">BV049868723</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20241016</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">240916s2024 xx a||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781071639498</subfield><subfield code="9">978-1-0716-3949-8</subfield></datafield><datafield tag="024" ind1="3" ind2=" "><subfield code="a">9781071639498</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1455417545</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV049868723</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29T</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Memory B-cells</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Kim L. Good-Jacobson</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Humana Press</subfield><subfield code="c">[2024</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">xi, 252 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">254 mm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">2826</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">With online files/update. - This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Memory B-Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Using ex vivo tonsil organoids to study memory B cells.- Ultrasound Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to assess B cell responses to Vaccination.- Human B cell receptor repertoire sequencing.- Visualising epigenetic modifications and nuclear bodies by immunofluorescence staining in naïve, activated and memory B cell subsets.- Measuring B cell Chromatin Accessibility by One-Step ATAC-seq.- Epigenomic profiling of B cell subsets by CUT&Tag.- In vivo CRISPR/Cas9-mediated gene ablation in murine B cells.- Isolation of rare antigen-specific memory B cells via antigen tetramers.- Detection of lymphocytic choriomeningitis virus-specific memory B cells using antigen tetramers.- B cell receptor transgenic mice as tools to study memory B cells.- ELISpots for detecting antigen-specific memory B cells in Infection or Autoimmunity.- Intracellular flow cytometry ("phosphoflow") to assess signal transduction in rare populations such as memory B cell subsets and plasma cells.- Quantifying human naïve B cell proliferation kinetics and differentiation in controlled in vitro cell culture.- Flow cytometric identification of human IgE+ B lineage subsets.- Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencing.-Assessing the survival of human plasma cells ex vivo.- Conversion of mouse-derived hybridomas to Tasmanian devil recombinant IgG antibodies.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bicssc</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bicssc</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bisacsh</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Experimental immunology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Vaccines—Biotechnology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biomaterials</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biology—Technique</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Hardcover, Softcover / Biologie/Sonstiges</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Good-Jacobson, Kim L.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="z">978-1-0716-3950-4</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">2826</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">2826</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-035208248</subfield></datafield></record></collection> |
| id | DE-604.BV049868723 |
| illustrated | Illustrated |
| indexdate | 2024-12-06T13:10:32Z |
| institution | BVB |
| isbn | 9781071639498 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-035208248 |
| oclc_num | 1455417545 |
| open_access_boolean | |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | xi, 252 Seiten Illustrationen, Diagramme 254 mm |
| publishDate | 2024 |
| publishDateSearch | 2024 |
| publishDateSort | 2024 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Memory B-cells methods and protocols edited by Kim L. Good-Jacobson New York, NY Humana Press [2024 xi, 252 Seiten Illustrationen, Diagramme 254 mm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2826 With online files/update. - This volume details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Memory B-Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field Using ex vivo tonsil organoids to study memory B cells.- Ultrasound Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to assess B cell responses to Vaccination.- Human B cell receptor repertoire sequencing.- Visualising epigenetic modifications and nuclear bodies by immunofluorescence staining in naïve, activated and memory B cell subsets.- Measuring B cell Chromatin Accessibility by One-Step ATAC-seq.- Epigenomic profiling of B cell subsets by CUT&Tag.- In vivo CRISPR/Cas9-mediated gene ablation in murine B cells.- Isolation of rare antigen-specific memory B cells via antigen tetramers.- Detection of lymphocytic choriomeningitis virus-specific memory B cells using antigen tetramers.- B cell receptor transgenic mice as tools to study memory B cells.- ELISpots for detecting antigen-specific memory B cells in Infection or Autoimmunity.- Intracellular flow cytometry ("phosphoflow") to assess signal transduction in rare populations such as memory B cell subsets and plasma cells.- Quantifying human naïve B cell proliferation kinetics and differentiation in controlled in vitro cell culture.- Flow cytometric identification of human IgE+ B lineage subsets.- Identification of IgG1 and IgG3 Allotypes by PCR and Sanger Sequencing.-Assessing the survival of human plasma cells ex vivo.- Conversion of mouse-derived hybridomas to Tasmanian devil recombinant IgG antibodies. bicssc bisacsh Experimental immunology Vaccines—Biotechnology Biomaterials Biology—Technique Hardcover, Softcover / Biologie/Sonstiges Good-Jacobson, Kim L. edt Erscheint auch als Online-Ausgabe 978-1-0716-3950-4 Methods in molecular biology 2826 (DE-604)BV035362695 2826 |
| spellingShingle | Memory B-cells methods and protocols Methods in molecular biology bicssc bisacsh Experimental immunology Vaccines—Biotechnology Biomaterials Biology—Technique |
| title | Memory B-cells methods and protocols |
| title_auth | Memory B-cells methods and protocols |
| title_exact_search | Memory B-cells methods and protocols |
| title_full | Memory B-cells methods and protocols edited by Kim L. Good-Jacobson |
| title_fullStr | Memory B-cells methods and protocols edited by Kim L. Good-Jacobson |
| title_full_unstemmed | Memory B-cells methods and protocols edited by Kim L. Good-Jacobson |
| title_short | Memory B-cells |
| title_sort | memory b cells methods and protocols |
| title_sub | methods and protocols |
| topic | bicssc bisacsh Experimental immunology Vaccines—Biotechnology Biomaterials Biology—Technique |
| topic_facet | bicssc bisacsh Experimental immunology Vaccines—Biotechnology Biomaterials Biology—Technique |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT goodjacobsonkiml memorybcellsmethodsandprotocols |