Flow cytometry protocols:
This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the proc...
Gespeichert in:
| 1. Verfasser: | |
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| Weitere Verfasser: | |
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2024]
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| Ausgabe: | Fifth edition |
| Schriftenreihe: | Methods in molecular biology
2779 |
| Schlagworte: | |
| Zusammenfassung: | This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the process of panel design and iterative optimization for spectral flow cytometry; novel methodologies such as image-enabled cell sorting, co-staining of fluorochrome-conjugated and oligonucleotide-conjugated antibodies, and screening for cell type selective probes; and a look at the achievements made in the clinical setting for both flow and mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Flow Cytometry Protocols, Fifth Edition is a valuable resource for researchers and scientists who are interested in continuing or expanding their knowledge of this developing field |
| Beschreibung: | Flow Cytometry: The Glass Is Half Full.- Basic Principles of Flow Cytometer Operation: The Make Your Own Flow Cytometer.- Lasers Sources for Traditional and Spectral Flow Cytometry.- How to Measure "Spillover Spread".- Comprehensive Immunophenotyping by Polychromatic Cytometry.- Panel Design and Optimization for Full Spectrum Flow Cytometry.- Practicalities of Cell Sorting.- Image-Enabled Cell Sorting Using the BD CellView Technology.- Monitoring Cell Proliferation by Dye Dilution: Considerations for Panel Design.- Multiparametric Analysis of Apoptosis by Flow Cytometry.- Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19.- CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting.- Co-Staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-seq.- Discovery of Live Cell Selective Fluorescent Probesand Elucidation of Their Mechanisms: Case Study of B Cell Selective Probe CDgB.- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.- Using Artificial Intelligence to Interpret Clinical Flow Cytometry Data Sets for Automated Disease Diagnosis and/or Monitoring.- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.- CyTOF Intracellular Cytokine Assays for Antigen-Specific T Cells.- Imaging Mass Cytometry for In Situ Immune Profiling.- Cytometry in High-Containment Laboratories. |
| Beschreibung: | xv, 458 Seiten Illustrationen, Diagramme 254 mm |
| ISBN: | 9781071637371 |
Internformat
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| 500 | |a Flow Cytometry: The Glass Is Half Full.- Basic Principles of Flow Cytometer Operation: The Make Your Own Flow Cytometer.- Lasers Sources for Traditional and Spectral Flow Cytometry.- How to Measure "Spillover Spread".- Comprehensive Immunophenotyping by Polychromatic Cytometry.- Panel Design and Optimization for Full Spectrum Flow Cytometry.- Practicalities of Cell Sorting.- Image-Enabled Cell Sorting Using the BD CellView Technology.- Monitoring Cell Proliferation by Dye Dilution: Considerations for Panel Design.- Multiparametric Analysis of Apoptosis by Flow Cytometry.- Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19.- CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting.- Co-Staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-seq.- Discovery of Live Cell Selective Fluorescent Probesand Elucidation of Their Mechanisms: Case Study of B Cell Selective Probe CDgB.- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.- Using Artificial Intelligence to Interpret Clinical Flow Cytometry Data Sets for Automated Disease Diagnosis and/or Monitoring.- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.- CyTOF Intracellular Cytokine Assays for Antigen-Specific T Cells.- Imaging Mass Cytometry for In Situ Immune Profiling.- Cytometry in High-Containment Laboratories. | ||
| 520 | |a This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the process of panel design and iterative optimization for spectral flow cytometry; novel methodologies such as image-enabled cell sorting, co-staining of fluorochrome-conjugated and oligonucleotide-conjugated antibodies, and screening for cell type selective probes; and a look at the achievements made in the clinical setting for both flow and mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Flow Cytometry Protocols, Fifth Edition is a valuable resource for researchers and scientists who are interested in continuing or expanding their knowledge of this developing field | ||
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| id | DE-604.BV049798617 |
| illustrated | Illustrated |
| indexdate | 2024-10-15T10:04:07Z |
| institution | BVB |
| isbn | 9781071637371 |
| language | English |
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| physical | xv, 458 Seiten Illustrationen, Diagramme 254 mm |
| publishDate | 2024 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
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| spelling | Flow cytometry protocols edited by Teresa S. Hawley (Bethesda, MD, USA), Robert G. Hawley (Washington, DC, USA) Fifth edition New York, NY Humana Press [2024] xv, 458 Seiten Illustrationen, Diagramme 254 mm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2779 Flow Cytometry: The Glass Is Half Full.- Basic Principles of Flow Cytometer Operation: The Make Your Own Flow Cytometer.- Lasers Sources for Traditional and Spectral Flow Cytometry.- How to Measure "Spillover Spread".- Comprehensive Immunophenotyping by Polychromatic Cytometry.- Panel Design and Optimization for Full Spectrum Flow Cytometry.- Practicalities of Cell Sorting.- Image-Enabled Cell Sorting Using the BD CellView Technology.- Monitoring Cell Proliferation by Dye Dilution: Considerations for Panel Design.- Multiparametric Analysis of Apoptosis by Flow Cytometry.- Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19.- CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting.- Co-Staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-seq.- Discovery of Live Cell Selective Fluorescent Probesand Elucidation of Their Mechanisms: Case Study of B Cell Selective Probe CDgB.- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.- Using Artificial Intelligence to Interpret Clinical Flow Cytometry Data Sets for Automated Disease Diagnosis and/or Monitoring.- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.- CyTOF Intracellular Cytokine Assays for Antigen-Specific T Cells.- Imaging Mass Cytometry for In Situ Immune Profiling.- Cytometry in High-Containment Laboratories. This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the process of panel design and iterative optimization for spectral flow cytometry; novel methodologies such as image-enabled cell sorting, co-staining of fluorochrome-conjugated and oligonucleotide-conjugated antibodies, and screening for cell type selective probes; and a look at the achievements made in the clinical setting for both flow and mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Flow Cytometry Protocols, Fifth Edition is a valuable resource for researchers and scientists who are interested in continuing or expanding their knowledge of this developing field bicssc bisacsh Biochemistry Cytology—Technique Durchflusscytometrie (DE-588)4226628-2 gnd rswk-swf Hardcover, Softcover / Biologie/Sonstiges Durchflusscytometrie (DE-588)4226628-2 s DE-604 Hawley, Teresa S. (DE-588)1012521575 edt Hawley, Robert G. (DE-588)1175042609 edt Erscheint auch als Online-Ausgabe 978-1-0716-3738-8 Methods in molecular biology 2779 (DE-604)BV035362695 2779 |
| spellingShingle | Hawley, Teresa S. Flow cytometry protocols bicssc bisacsh Biochemistry Cytology—Technique Durchflusscytometrie (DE-588)4226628-2 gnd Methods in molecular biology |
| subject_GND | (DE-588)4226628-2 |
| title | Flow cytometry protocols |
| title_auth | Flow cytometry protocols |
| title_exact_search | Flow cytometry protocols |
| title_full | Flow cytometry protocols edited by Teresa S. Hawley (Bethesda, MD, USA), Robert G. Hawley (Washington, DC, USA) |
| title_fullStr | Flow cytometry protocols edited by Teresa S. Hawley (Bethesda, MD, USA), Robert G. Hawley (Washington, DC, USA) |
| title_full_unstemmed | Flow cytometry protocols edited by Teresa S. Hawley (Bethesda, MD, USA), Robert G. Hawley (Washington, DC, USA) |
| title_short | Flow cytometry protocols |
| title_sort | flow cytometry protocols |
| topic | bicssc bisacsh Biochemistry Cytology—Technique Durchflusscytometrie (DE-588)4226628-2 gnd |
| topic_facet | bicssc bisacsh Biochemistry Cytology—Technique Durchflusscytometrie |
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