The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model:
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
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Münster
2022
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| Beschreibung: | 97 Blätter Illustrationen, Diagramme 30 cm |
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| 100 | 1 | |a Grenzheuser, Julia Chiara |d 1998- |e Verfasser |0 (DE-588)1308337027 |4 aut | |
| 245 | 1 | 0 | |a The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |c vorgelegt von Grenzheuser, Julia Chiara |
| 264 | 1 | |a Münster |c 2022 | |
| 300 | |a 97 Blätter |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
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| 502 | |b Dissertation |c Westfälische Wilhelms-Universität Münster |d 2022 | ||
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Datensatz im Suchindex
| _version_ | 1806142543704883200 |
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INDEX
1
INTRODUCTION
.
1
1.1
PULMONARY
INFLAMMATION
AND
ARDS
.
1
1.2
THE
IMMUNE
SYSTEM
.
2
1.3
PMN
TRANSMIGRATION
FROM
THE
VASCULAR
SYSTEM
TO
THE
SITE
OF
INFLAMMATION
.
4
1.3.1
CHARACTERISTICS
OF
THE
ALVEOLAR
ENDOTHELIUM
.
4
1.3.2
LEUKOCYTE
RECRUITMENT
CASCADE
AND
TRANSMIGRATION
.
4
1.3.3
ROLE
OF
THE
ALVEOLAR
EPITHELIUM
IN
HOST
DEFENSE
.
6
1.3.4
CHEMOTAXIS
OF
PMNS
.
7
1.3.4.1
DETECTION
OF
PATHOGENS
AND
INITIATION
OF
AN
IMMUNE
RESPONSE
.
7
1.3.4.2
CHEMOKINES
AND
THEIR
RECEPTORS
.
8
1.3.4.3
CHEMOKINE
RECEPTOR
CXCR2
.
9
1.4
AIMS
OF
THIS
STUDY
.
11
2
MATERIALS
AND
METHODS
.
12
2.1
MATERIALS
.
12
2.1.1
ANIMALS
.
12
2.1.2
CONSUMABLES
.
12
2.1.3
CHEMICALS
AND
REAGENTS
.
14
2.1.4
ANTIBODIES
.
17
2.1.4.1
FLUORESCENCE-COUPLED
ANTIBODIES
.
17
2.1.4.2
PRIMARY
ANTIBODIES
.
18
2.1.4.3
FLUORESCENCE-COUPLED
SECONDARY
ANTIBODIES
.
19
2.1.5
EQUIPMENT
.
19
2.1.6
BUFFERS
AND
SOLUTIONS
.
21
2.1.7
MEDIA
.22
2.1.8
SOFTWARE
.
23
2.2
METHODS
.
24
2.2.1
ISOLATION
OF
PRIMARY
MURINE
LUNG
MICROVASCULAR
ENDOTHELIAL
CELLS
.
24
2.2.2
SORTING
OF
PRIMARY
MLMVECS
.
25
2.2.3
SPLITTING
OF
PRIMARY
MLMVECS
.
26
2.2.4
ISOLATION
OF
PRIMARY
MURINE
ALVEOLAR
EPITHELIAL
CELLS
TYPE
1
AND
2
.
27
2.2.5
ISOLATION
OF
PMNS
.
29
2.2.6
TRANSMIGRATION
ASSAY
.30
2.2.7
DEXTRAN
ASSAY
.33
2.2.8
PHALLOIDIN
STAINING
OF
SINGLE
CELL
LAYERS
.
34
2.2.9
FLOW
CYTOMETRY
.
35
2.2.9.1
STAINING
OF
EXTRACELLULAR
PROTEINS
.35
2.2.9.2
STAINING
OF
INTRACELLULAR
PROTEINS
.37
2.2.9.3
FLOW
CYTOMETRY
ANALYSIS
.37
2.2.10
STATISTICAL
ANALYSIS
.
39
3
RESULTS
.
40
3.1
THE
GENETIC
DEPLETION
OF
CXCR2
HAS
NO
IMPACT
ON
MORPHOLOGY
OR
KEY
PROTEINS
OF
MLMVECS
AND
ALVEOLAR
EPITHELIAL
CELLS
.
40
3.1.1
KO
MLMVECS
RESEMBLE
WT
MLMVECS
IN
MORPHOLOGY
AND
TYPICAL
SURFACE
MARKER
EXPRESSION
.
40
3.1.2
DEPLETION
OF
CXCR2
DOES
NOT
INFLUENCE
THE
PHENOTYPE
OF
ALVEOLAR
EPITHELIAL
CELLS
TYPE
1
AND
2
.
46
3.1.2.1
ALVEOLAR
EPITHELIAL
CELLS
ON
DAY
2
.
46
3.1.2.2
ALVEOLAR
EPITHELIAL
CELLS
ON
DAY
7
.
52
3.2
VERIFICATION
OF
LAYER
CONFLUENCY
.
58
3.3
CXCR2
DEFICIENCY
ON
MLMVECS
AND
ALVEOLAR
EPITHELIAL
CELLS
RESULTS
IN
AN
IMPAIRED
PMN
RECRUITMENT
.
60
4
DISCUSSION
.
63
4.1
CXCR2++
AND
CXCR2-
ISOLATED
CELLS
SHOWED
REGULAR
PROTEIN
COMPOSITIONS
FOR
A
PHYSIOLOGICAL
IMMUNE
RESPONSE
.63
4.1.1
THE
ALVEOLAR
MICROVASCULAR
ENDOTHELIUM
.
64
4.1.2
THE
ALVEOLAR
EPITHELIUM
.66
4.2
.
ENDOTHELIAL
AND
EPITHELIAL
CXCR2
AFFECTS
THE
TRANSPORT
OF
CHEMOKINES
FOR
PMN
ATTRACTION
.
68
4.3
SUMMARY
AND
OUTLOOK
.
71
5
INDEX
OF
ABBREVIATIONS
.73
6
REFERENCES
.75
7
LIST
OF
FIGURES
.88
8
LIST
OF
TABLES
.
94
9
LEBENSLAUF
.
95
10
DANKSAGUNG
.
96
11
GENEHMIGUNG
.97 |
| any_adam_object | 1 |
| author | Grenzheuser, Julia Chiara 1998- |
| author_GND | (DE-588)1308337027 |
| author_facet | Grenzheuser, Julia Chiara 1998- |
| author_role | aut |
| author_sort | Grenzheuser, Julia Chiara 1998- |
| author_variant | j c g jc jcg |
| building | Verbundindex |
| bvnumber | BV049770386 |
| ctrlnum | (OCoLC)1428532835 (DE-599)DNB1309912572 |
| discipline | Medizin |
| format | Thesis Book |
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| indexdate | 2024-08-01T00:22:35Z |
| institution | BVB |
| language | English |
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| physical | 97 Blätter Illustrationen, Diagramme 30 cm |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
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| spelling | Grenzheuser, Julia Chiara 1998- Verfasser (DE-588)1308337027 aut The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model vorgelegt von Grenzheuser, Julia Chiara Münster 2022 97 Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische Wilhelms-Universität Münster 2022 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1309912572/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035111593&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20240308 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Grenzheuser, Julia Chiara 1998- The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |
| subject_GND | (DE-588)4113937-9 |
| title | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |
| title_auth | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |
| title_exact_search | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |
| title_full | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model vorgelegt von Grenzheuser, Julia Chiara |
| title_fullStr | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model vorgelegt von Grenzheuser, Julia Chiara |
| title_full_unstemmed | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model vorgelegt von Grenzheuser, Julia Chiara |
| title_short | The role of pulmonary endothelial and epithelial CXCR2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |
| title_sort | the role of pulmonary endothelial and epithelial cxcr2 in leukocyte recruitment during pulmonary inflammation in a murine in vitro transmigration model |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1309912572/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035111593&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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