Introduction to biological imaging:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Hoboken, NJ
Wiley
2024
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xv, 330 Seiten Illustrationen, Diagramme |
| ISBN: | 9781119705949 1119705940 |
Internformat
MARC
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| 100 | 1 | |a Auer, Manfred |d 1966- |e Verfasser |0 (DE-588)120955989 |4 aut | |
| 245 | 1 | 0 | |a Introduction to biological imaging |c Manfred Auer |
| 246 | 1 | 3 | |a Biological imaging |
| 264 | 1 | |a Hoboken, NJ |b Wiley |c 2024 | |
| 300 | |a xv, 330 Seiten |b Illustrationen, Diagramme | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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Datensatz im Suchindex
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Contents Preface xül 1 1.1 1.1.1 1.1.2 1.1.3 1.2 1,2.1 1.2.2 Introduction - A Brief History of Imaging 1 Spatiotemporally Organization of Biological Processes 2 Metabolism 2 Developmental Biology 2 Disease Mechanisms 2 Why Imaging? 3 A Very Brief History of Cellular Biological Imaging 3 A Very Brief History of Macromolecular Biological Imaging 5 2.1 2.1.1 2.1.2 2.1.3 2.1.4 2.1.5 2.1.6 2.2 2.2.1 2.2.2 2.2.3 2.2.4 2.2.5 2.2.6 2.2.7 2.3 2.3.1 2.3.2 2.3.3 2.3.4 Basics of Atomic and Molecular Physics 9 Matter and Energy 9 Standard Model of Particle Physics 9 Atomic Structure 11 Periodic Table of Elements 16 Transitions of Electrons 17 Molecular Orbitals 17 Forces and Bonds Between Neighboring Atoms Electromagnetic Spectrum 21 Gamma Rays 22 X-Rays 22 Ultraviolet Radiation 22 Visible Light 22 Infrared Radiation 23 Microwave Radiation 24 Radio Wave Radiation 24 Interaction of Radiation with Matter 24 High-Energy Photons 24 High-Energy Particles 25 Middle-Energy Photons 25 Low-Energy Photons 26 3 Basics of Optics 2 3.1 3.2 3.3 3.3.1 3.3.2 3.3.3 33 Properties of Light 33 Classical Optics 33 Interaction of Light with Matter 34 Reflection 34 Refraction 34 Dispersion 35 18
vîii J Contents 3.4 3.5 3.6 3.7 3.8 3.9 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 Lenses 35 Numerical Aperture 35 Scattering 36 Interference 37 Diffraction 37 Airy Disk 39 Point-Spread Function 39 Resolution 40 Aberrations 41 Polarization 41 Photoelectric Effect 42 Valence Electron Transitions 42 Vibrational and Rotational Transitions 43 Stimulated Emission 43 4 Basics of Fourier “Math” 45 5 5.1 5.2 5.2.1 5.2.2 5.2.3 5.2.4 5.2.5 5.2.6 5.2.7 5.2.8 5.2.9 5.2.10 5.2.11 5.2.12 5.2.13 5.3 5.4 5.5 5.6 5.6.1 5.6.2 5.6.3 5.6.4 5.6.5 5.7 5.8 5.9 5.10 5.11 5.12 5.13 5.13.1 5.13.2 5.13.3 5.13.4 Basics of Imaging and Image Formation 51 Goals of Imaging 51 Source of Radiation 52 Gamma-Rays 52 X-Rays 53 UV-Rays/Visible Light Rays/Near-IR Light Rays 53 IR Radiation 55 Microwave Radiation 55 Radio Wave Radiation 55 Particle Radiation 55 Particle Radiation: Electrons 56 Particle Radiation: Positrons 57 Particle Radiation: Alpha-Radiation/Helium Nuclei 57 Particle Radiation: Ion Beams 57 Particle Radiation: Neutrons 57 Particle Radiation: Protons 57 Beam Collimation 57 Lenses 58 Lens-Free Imaging/Near Field Imaging 59 Signal Detection and Detectors 60 What Are the Different Types of Radiation That Need to Be Detected? Detection of Particle Radiation 61 Detection of Ionizing Electromagnetic Radiation 62 Detection of Non-Ionizing Electromagnetic Radiation 63 Principles of Signal Detection 64 Modulation-, Contrast-, and Optical-Transfer Function 69 Field of View, Coverage 70 Spot-Scan Versus Flood-Beam Imaging 71 Radiation Damage 72 Spectroscopic Imaging 72 Multidimensional and Multi-channel
Imaging 73 Sample Preparation Requirements 75 Macromolecular 3D Structure 75 Subcellular, Cellular, and Tissue 3D Architecture 76 Dynamic Localization of Macromolecules in Cells and Tissues 77 Chemical Inventory of Cells and Tissues 77 60
5.13.5 5.14 5.15 5.16 5.17 5.18 Medical Imaging of Tissues and Organs 78 Labels and Labeling 79 Label-Free Imaging 81 2D Versus 3D Imaging 82 Structural Versus Functional Imaging 85 Correlative Imaging 85 6 6.1 6.2 6.3 6.4 6.5 6.6 6.6.1 6.6.2 6.6.3 6.7 6.8 6.9 6.10 6.11 6.12 6.12.1 6.12.2 6.12.3 6.12.4 6.12.5 6.12.6 6.13 6.13.1 6.13.2 6.13.3 6.14 6.15 Basics of Image Processing and Image Analysis 87 Overview 87 Characteristics of Digital Images 89 Compression of Image Data 96 Metadata 98 Spatial, Temporal and Spectral Resolution 99 Image Intensity Values 99 Contrast 99 Histogram 100 False-Color Representation and Lookup Tables 101 Raster Graphics Versus Vector Graphics 101 Simple Image Processing Operations 102 Image Restoration 104 Image Enhancement 104 Image Reconstruction 109 Visualization 109 Introduction to Scientific Visualization 109 Visualization Software Examples 113 3D Volume Image Data 114 Visualizing Macromolecular Imaging Data 115 Visualizing Cellular Imaging Data 116 A Few More Points Worth Noting 119 Image Segmentation 119 Map Density Value-Based Segmentation 120 Manual Segmentation - Creating Regions of Interest 121 Automated Segmentation 122 Making Changes to Object Selection: Dilation, Erosion, Closing, Filling Holes Measurements/Quantitative Analysis 131 7 7.1 7.1.1 7.1.2 7.1.3 7.1.4 7.1.5 7.1.6 7.1.7 7.1.8 7.1.9 7.1.10 7.2 7.2.1 7.2.2 7.2.3 7.2.4 Techniques - Macromolecular Structure Determination X-Ray Crystallography 135 Overview 135 Historical Perspective/Milestones 137 Theoretical Background 138 Instrumentation/Experimental Setup 143 Data Collection
146 Data Analysis and Phasing 147 Map Calculation and Model Building 148 Refinement and Validation 148 Visualization 149 Outlook 149 Macromolecular Cryo-Electron Microscopy 150 Overview 150 Historical Perspective/Milestones 152 Theoretical Background 162 Instrumentation/Experimental Setup 165 135 131
XI Contents 7.2.5 7.2.6 7.2.7 7.2.8 7.3 7.3.1 7.3.2 7.3.3 7.3.4 7.3.5 7.4 7.4.1 7.4.2 7.5 Sample Preparation and Data Collection 168 Data Analysis 169 Map Calculation and Resolution Estimates 175 Visualization and Model Building 176 NMR Spectroscopy 177 Overview 177 Historical Perspective/Milestones 178 Theoretical Background 179 Instrumentation and Experimental Setup 181 Data Collection and Analysis 181 Small Angle Scattering - SAXS and SANS 184 Small-Angle X-Ray Scattering (SAXS) 184 Small-Angle Neutron Scattering (SANS) 184 Atomic Force Microscopy (AFM) 185 8 8.1 8.2 8.2.1 8.2.2 8.2.3 8.2.4 8.2.5 8.3 8.3.1 8.3.2 8.3.3 8.3.4 8.3.5 8.4 8.4.1 8.4.2 8.4.3 8.4.4 8.4.5 8.5 8.5.1 8.5.2 8.5.3 8.5.4 8.5.5 8.6 8.6.1 8.6.2 8.6.3 8.6.4 8.6.5 8.7 8.7.1 8.7.2 8.7.3 8.7.4 Techniques - Cell and Tissue Architectural Imaging 189 Overview 189 Historical Perspective/Milestones 191 Transmission Electron Microscopy (ТЕМ) Imaging 195 Scanning Electron Microscopy (SEM) Imaging 196 Helium Ion Microscopy Imaging 196 X-Ray Microscopy Tomography Imaging 197 Optical/Fluorescence Light Microscopy Imaging 199 Theoretical Background 199 Contrast Generation in Transmission Electron Microscopy 199 Contrast Generation in Scanning Electron Microscopy (SEM) 200 Contrast Generation in Helium Ion Microscopy (HelM) 201 Contrast Generation in X-Ray Microscopy Tomography (XRMT) 201 3D Reconstructions of Pleiomorphic Objects 203 Sample Preparation 205 Electron Microcopy 205 Need for Thin Samples 205 Conventional ТЕМ Sample Preparation 205 Cryogenic EM Sample Preparation 206 Sample Preparation for cryo-XRMT 208
Instrumentation 208 Radiation Source 209 Electron/lon/X-Ray Optical System 210 Detectors 211 Specimen Stage 211 Vacuum System 213 Data Collection and 3D Reconstruction 214 Serial Section ТЕМ 214 Serial Section SEM 215 Serial Block Face SEM 215 Focused Ion Beam (Dual Beam) SEM 215 X-Ray Microscopy Tomography 216 Data Visualization and Analysis 217 Visualization of Cellular Sceneries 218 Segmentation/Feature Extraction 219 Model Building and (Quantitative) Analysis 221 Deriving Biological Meaning Through Interpretation 222
9 9.1 9.2 9.2.1 9.2.2 9.2.3 9.2.4 9.3 9.4 9.4.1 9.4.2 9.4.3 9.4.4 9.5 9.5.1 9.5.2 9.5.3 9.6 9.6.1 9.6.2 9.6.3 9.7 9.7.1 9.7.2 9.7.3 9.7.4 9.7.5 9.8 9.9 9.10 9.11 9.11.1 9.11.2 9.11.3 9.11.4 9.11.5 9.11.6 9.11.7 9.11.8 9.12 9.12.1 9.12.2 9.12.3 9.13 9.13.1 9.13.2 9.14 9.15 9.15.1 9.15.2 9.15.3 9.15.4 Techniques - Cell and Tissue Characterization and Localization Imaging 225 Overview 225 Historical Perspective/Milestones 226 What Is Light? 226 Optical Instruments and Microscopy 228 Application of Microscopy to Biological Samples 230 Fluorescence Microscopy 231 Theoretical Background 232 Instrumentation/Experimental Setup 235 Frame, Stage, and Sample Holders 235 Light Source and Illumination 237 Optical Path 238 Detector 239 Data Collection 241 Choosing the Right Microscopy Technique 241 White Light Imaging 241 Fluorescence Microscopy 243 Fluorophore Considerations 244 Synthetic Organic Dyes 244 Quantum Dots 246 Immuno-Affinity-Based Labeling 246 Genetically Encoded Fluorescent Proteins 247 Intrinsically Fluorescent Proteins 247 Photoactivatable Fluorescent Proteins 248 Photoconvertible Fluorescent Proteins 248 Photoswitchable Fluorescent Proteins 248 Extrinsically Fluorescent Proteins 248 RNA/DNA - Fluorescent Probes 248 Fluorescent Imaging Types 250 Detangling the Many Fluorescence Imaging Approaches 250 Improve Axial Resolution and Reduce Phototoxicity 251 Epifluorescence Microscopy 251 Deconvolution Microscopy 251 Confocal Laser Scanning Microscopy 252 Spinning Disk Confocal Microscope 252 4Pi and Confocal Theta Microscopy 252 Two-Photon Microscopy 253 Single Plane
Illumination Microscopy (SPIM) 255 Total-Internal Reflection Fluorescence (TIRF) Microscopy 256 Superresolution Imaging - Breaking the Resolution Barrier 257 Photoactivation 257 Photoconversion 257 Photoswitching 258 Superresolution Imaging 258 Single-Molecule Localization Microscopy 258 Patterned Illumination Microscopy 260 Near-Field Scanning Microscopy 261 Molecular Dynamics Study of Macromolecules 261 Fluorescence Recovery After Photobleaching (FRAP) Microscopy 261 Fluorescence Loss in Photobleaching (FLIP) Microscopy 262 Fluorescence Speckle Microscopy (FSM) 262 Förster Resonance Energy Transfer Microscopy 262
xiî J Contents 9.15.5 9.16 9.17 Fluorescence Lifetime Imaging Microscopy 264 Visualization 264 Data Analysis 265 10 10.1 10.2 10.2.1 10.2.2 10.2.3 10.2.4 10.3 10.4 10.4.1 10.4.2 10.4.3 10.4.4 Techniques - Chemical Imaging at theCell and Tissue Level 267 X-Ray Microanalysis: X-Ray Fluorescence Microscopy (XFM), Energy-Dispersive X-Ray Spectroscopy (EDX), Wavelength-Dispersive X-ray Spectroscopy (WDS) 269 Raman Imaging 271 Overview 271 Theoretical Background 272 Experimental Design 276 Data Analysis 276 Fourier-Transform Infrared (FTIR) Imaging 277 Mass Spectrometry 279 Overview 279 Historical Perspective 282 Instrumentation 283 Data Analysis 289 11 11.1 11.2 11.3 11.4 11.5 11.6 11.7 Techniques-Tissue and Organ Medical Imaging 291 Positron Emission Tomography 292 Computed (Axial) Tomography 294 Magnetic Resonance Imaging (MRI) 294 Medical Ultrasound 296 Bioluminescence 297 Optical Coherence Tomography 297 Histology/Histopathology 298 12 12.1 12.2 12.2.1 12.2.2 12.2.3 12.2.4 12.2.5 12.2.6 12.2.7 12.2.8 12.2.9 12.3 12.4 12.5 12.6 12.7 12,8 The Future of Bioimaging 301 Future of Macromolecular Imaging 301 Future of (Sub)Cellular and Tissue Imaging 303 Light Microscopy Versus Electron Microscopy 303 Cryo-Preservation in EM Sample Preparation 304 FIB Milling Sample Preparation 304 Electron Tomography 304 Correlative Light and Electron Microscopy 305 Volume-SEM 305 Helium Ion Microscopy 305 Cryo-Soft-X-Ray Microscopy Tomography 306 Hard-X-Ray Microscopy Tomography 306 Future of Chemical Imaging 307 Future of Medical Imaging 307 Future of Image Processing and Analysis 308 Future of
Multiscale Imaging 310 Future of Multimodal Imaging - Correlative Imaging 313 Future of Information Integration - Multiscale, Multimodal, and Integrated Index 319 315 |
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| spelling | Auer, Manfred 1966- Verfasser (DE-588)120955989 aut Introduction to biological imaging Manfred Auer Biological imaging Hoboken, NJ Wiley 2024 xv, 330 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Bildgebendes Verfahren (DE-588)4006617-4 gnd rswk-swf Biologisches Material (DE-588)4145633-6 gnd rswk-swf Biologisches Material (DE-588)4145633-6 s Bildgebendes Verfahren (DE-588)4006617-4 s DE-604 Erscheint auch als Online-Ausgabe, EPUB 978-1-119-70595-6 Erscheint auch als Online-Ausgabe, PDF 978-1-119-70597-0 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=035038015&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | Introduction to biological imaging |
| title_alt | Biological imaging |
| title_auth | Introduction to biological imaging |
| title_exact_search | Introduction to biological imaging |
| title_full | Introduction to biological imaging Manfred Auer |
| title_fullStr | Introduction to biological imaging Manfred Auer |
| title_full_unstemmed | Introduction to biological imaging Manfred Auer |
| title_short | Introduction to biological imaging |
| title_sort | introduction to biological imaging |
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