Study on transport mechanism of m5C-edited mRNAs:
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|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Potsdam
November 2022
|
| Subjects: | |
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| Item Description: | Die Zahl "5" im Titel ist hochgestellt |
| Physical Description: | X, 133 Seiten Illustrationen, Diagramme 30 cm |
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MARC
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| 100 | 1 | |a Xu, Ying |d 1993- |e Verfasser |0 (DE-588)1281885304 |4 aut | |
| 245 | 1 | 0 | |a Study on transport mechanism of m5C-edited mRNAs |c von Ying Xu |
| 264 | 1 | |a Potsdam |c November 2022 | |
| 300 | |a X, 133 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
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| _version_ | 1804186458819395584 |
|---|---|
| adam_text | CONTENTS
ABSTRACT
..................................................................................................................................
I
ZUSAMMENFASSUNG
...............................................................................................................
II
ABBREVIATIONS
......................................................................................................................
IV
1.
INTRODUCTION
.......................................................................................................................
1
1.1
MESSENGER
RNA
(MRNA)
LONG-DISTANCE
TRANSPORT
.................................................
1
1.1.1
APPROACHES
USED
TO
IDENTIFY
MOBILE
RNAS
......................................................
2
1.1.2
CLASSES
OF
TRANSPORTED
RNAS
...........................................................................
6
1.1.3
DETERMINANTS
MEDIATING
MRNA
TRANSPORT
......................................................
12
1.1.4
SIGNALLING
FUNCTION
OF
MOBILE
RNAS
................................................................
18
1.2
M
5
C
MODIFIED
MRNAS
.............................................................................................
22
1.2.1
DETECTION
FOR
M
5
C
RNA
...................................................................................
23
1.2.2
REGULATORY
MECHANISM
OF
M
5
C
IN
MRNAS
...................................................
26
1.2.3
BIOLOGICAL
FUNCTION
OF
M
5
C
RNA
MODIFICATION
ON
MRNA
..............................
31
1.2.4
M
5
C
MRNAS
DETECTED
IN
PLANTS
......................................................................
33
1.3
AIM
OF
THIS
STUDY
......................................................................................................
35
2.
RESULTS
............................................................................................................................
36
2.1
IDENTIFICATION
OF
MOBILE
MRNAS
............................................................................
36
2.1.1
DIFFERENTIALLY
EXPRESSED
GENES
(DEGS)
BETWEEN
DNMT2
NSUN2B
MUTANT
AND
WILD-TYPE
......................................................................................................................
41
2.1.2
IDENTIFICATION
OF
MOBILE
TRANSCRIPTS
..................................................................
48
2.1.3
ANALYSIS
OF
MOBILE
TRANSCRIPTS
.........................................................................
49
2.1.4
MRNA
MOBILITY
IN
HYPOCOTYL-GRAFTED
A.
THALIANA
AT
DIFFERENT
DEVELOPMENTAL
STAGES
AND
DNMT2
NSUN2B
MUTANTS
.........................................................................58
2.2
POTENTIAL
ROLES
OF
SELECTED
RNA
BINDING
PROTEINS
(RBPS)
IN
TRANSPORT
............
69
2.2.1
SELECTION
OF
RNA
BINDING
PROTEINS
(RBPS)
....................................................
69
2.2.2
RNA
IMMUNOPRECIPITATION
(RIP)
OF
TCTP1,
HSC70.1,
ALY2
AND
ALY4...
71
2.2.3
CO-IMMUNOPRECIPITATION
(CO-IP)
OF
TCTP1,
HSC70.1,
ALY2
AND
ALY4
..
78
2.2.4
TCTP1
AND
GRP7
TRANSCRIPT
MOBILITY
IS
AFFECTED
IN
ALY2
AND/OR
ALY4
MUTANT
80
2.2.5
IDENTIFICATION
AND
ANALYSIS
OF
MOBILE
TRANSCRIPTS
IN
JUVENILE
ALY4
MUTANT...
87
3.
DISCUSSION
AND
OUTLOOK
................................................................................................
90
3.1
BIOLOGICAL
FUNCTION
OF
M
5
C
MODIFICATION
AND
STRESS
.............................................
90
3.2
VERIFICATION
OF
MOBILE
MRNAS
................................................................................
91
3.3
MRNA
MOBILITY
CHANGES
IN
ADULT
VERSUS
JUVENILE
PLANTS
......................................
93
3.4
POTENTIAL
FUNCTION
AND
MECHANISM
OF
ALY
PROTEINS
IN
MEDIATING
MRNA
TRANSPORT
..........................................................................................................................................
95
3.5
POTENTIAL
MECHANISM
OF
M
5
C
MODIFICATION
IN
MEDIATING
MRNA
TRANSPORT
........
97
4.
MATERIALS
AND
METHODS
................................................................................................
100
4.1
PLANT-RELATED
PROTOCOLS
..........................................................................................
100
4.1.1
BACTERIAL
STRAINS
AND
PLANT
LINES
USED
...........................................................
100
4.1.2
SEEDS
STERILIZATION
...........................................................................................
101
4.1.3
PLANT
GROWTH
....................................................................................................
101
4.1.4
AGROBACTERIUM
TRANSFORMATION
OF
ARABIDOPSIS
THALIANA
..............................
102
4.1.5
ARABIDOPSIS
HYPOCOTYL
GRAFTING
......................................................................
102
4.2
RNA-RELATED
PROTOCOLS
...........................................................................................
103
4.2.1
A.
THALIANA
RNA
EXTRACTION
............................................................................
103
4.2.2
REVERSE
TRANSCRIPTION
REACTION
......................................................................104
4.2.3
RT-PCR
...........................................................................................................
104
4.2.4
QUANTITATIVE
REAL
TIME
PCR
(QRT-PCR)
.......................................................
105
4.3
DNA-RELATED
PROTOCOLS
...........................................................................................
106
4.3.1
DNA
EXTRACTION
................................................................................................
106
4.3.2
GENOTYPING
BY
GENOMIC
PCR
........................................................................
107
4.4
IMMUNOPRECIPITATION
(IP)-RELATED
PROTOCOLS
.........................................................
107
4.4.1
RNA/CO-IMMUNOPRECIPITATION
(RIP/CO-IP)
..................................................
107
4.4.2
RIP-SEQ
DATA
ANALYSIS
....................................................................................
108
4.4.3
MASS
SPECTROMETRY
(MS)
ANALYSIS
................................................................108
4.4.4
PROTEIN
ISOLATION
AND
IMMUNOBLOT
DETECTION
...............................................109
4.5
MOBILITY
ANALYSIS
....................................................................................................109
4.6
MICROSCOPY
.............................................................................................................110
APPENDIX
...........................................................................................................................
111
APPENDIX
TABLE
1
.........................................................................................................
111
APPENDIX
TABLE
2
.........................................................................................................
112
APPENDIX
TABLE
3
.........................................................................................................
113
REFERENCES
........................................................................................................................
114
CURRICULUM
VITAE
...............................................................................................................
130
ACKNOWLEDGEMENTS
.........................................................................................................
132
STATEMENT
OF
AUTHORSHIP
..................................................................................................
133
|
| adam_txt |
CONTENTS
ABSTRACT
.
I
ZUSAMMENFASSUNG
.
II
ABBREVIATIONS
.
IV
1.
INTRODUCTION
.
1
1.1
MESSENGER
RNA
(MRNA)
LONG-DISTANCE
TRANSPORT
.
1
1.1.1
APPROACHES
USED
TO
IDENTIFY
MOBILE
RNAS
.
2
1.1.2
CLASSES
OF
TRANSPORTED
RNAS
.
6
1.1.3
DETERMINANTS
MEDIATING
MRNA
TRANSPORT
.
12
1.1.4
SIGNALLING
FUNCTION
OF
MOBILE
RNAS
.
18
1.2
M
5
C
MODIFIED
MRNAS
.
22
1.2.1
DETECTION
FOR
M
5
C
RNA
.
23
1.2.2
REGULATORY
MECHANISM
OF
M
5
C
IN
MRNAS
.
26
1.2.3
BIOLOGICAL
FUNCTION
OF
M
5
C
RNA
MODIFICATION
ON
MRNA
.
31
1.2.4
M
5
C
MRNAS
DETECTED
IN
PLANTS
.
33
1.3
AIM
OF
THIS
STUDY
.
35
2.
RESULTS
.
36
2.1
IDENTIFICATION
OF
MOBILE
MRNAS
.
36
2.1.1
DIFFERENTIALLY
EXPRESSED
GENES
(DEGS)
BETWEEN
DNMT2
NSUN2B
MUTANT
AND
WILD-TYPE
.
41
2.1.2
IDENTIFICATION
OF
MOBILE
TRANSCRIPTS
.
48
2.1.3
ANALYSIS
OF
MOBILE
TRANSCRIPTS
.
49
2.1.4
MRNA
MOBILITY
IN
HYPOCOTYL-GRAFTED
A.
THALIANA
AT
DIFFERENT
DEVELOPMENTAL
STAGES
AND
DNMT2
NSUN2B
MUTANTS
.58
2.2
POTENTIAL
ROLES
OF
SELECTED
RNA
BINDING
PROTEINS
(RBPS)
IN
TRANSPORT
.
69
2.2.1
SELECTION
OF
RNA
BINDING
PROTEINS
(RBPS)
.
69
2.2.2
RNA
IMMUNOPRECIPITATION
(RIP)
OF
TCTP1,
HSC70.1,
ALY2
AND
ALY4.
71
2.2.3
CO-IMMUNOPRECIPITATION
(CO-IP)
OF
TCTP1,
HSC70.1,
ALY2
AND
ALY4
.
78
2.2.4
TCTP1
AND
GRP7
TRANSCRIPT
MOBILITY
IS
AFFECTED
IN
ALY2
AND/OR
ALY4
MUTANT
80
2.2.5
IDENTIFICATION
AND
ANALYSIS
OF
MOBILE
TRANSCRIPTS
IN
JUVENILE
ALY4
MUTANT.
87
3.
DISCUSSION
AND
OUTLOOK
.
90
3.1
BIOLOGICAL
FUNCTION
OF
M
5
C
MODIFICATION
AND
STRESS
.
90
3.2
VERIFICATION
OF
MOBILE
MRNAS
.
91
3.3
MRNA
MOBILITY
CHANGES
IN
ADULT
VERSUS
JUVENILE
PLANTS
.
93
3.4
POTENTIAL
FUNCTION
AND
MECHANISM
OF
ALY
PROTEINS
IN
MEDIATING
MRNA
TRANSPORT
.
95
3.5
POTENTIAL
MECHANISM
OF
M
5
C
MODIFICATION
IN
MEDIATING
MRNA
TRANSPORT
.
97
4.
MATERIALS
AND
METHODS
.
100
4.1
PLANT-RELATED
PROTOCOLS
.
100
4.1.1
BACTERIAL
STRAINS
AND
PLANT
LINES
USED
.
100
4.1.2
SEEDS
STERILIZATION
.
101
4.1.3
PLANT
GROWTH
.
101
4.1.4
AGROBACTERIUM
TRANSFORMATION
OF
ARABIDOPSIS
THALIANA
.
102
4.1.5
ARABIDOPSIS
HYPOCOTYL
GRAFTING
.
102
4.2
RNA-RELATED
PROTOCOLS
.
103
4.2.1
A.
THALIANA
RNA
EXTRACTION
.
103
4.2.2
REVERSE
TRANSCRIPTION
REACTION
.104
4.2.3
RT-PCR
.
104
4.2.4
QUANTITATIVE
REAL
TIME
PCR
(QRT-PCR)
.
105
4.3
DNA-RELATED
PROTOCOLS
.
106
4.3.1
DNA
EXTRACTION
.
106
4.3.2
GENOTYPING
BY
GENOMIC
PCR
.
107
4.4
IMMUNOPRECIPITATION
(IP)-RELATED
PROTOCOLS
.
107
4.4.1
RNA/CO-IMMUNOPRECIPITATION
(RIP/CO-IP)
.
107
4.4.2
RIP-SEQ
DATA
ANALYSIS
.
108
4.4.3
MASS
SPECTROMETRY
(MS)
ANALYSIS
.108
4.4.4
PROTEIN
ISOLATION
AND
IMMUNOBLOT
DETECTION
.109
4.5
MOBILITY
ANALYSIS
.109
4.6
MICROSCOPY
.110
APPENDIX
.
111
APPENDIX
TABLE
1
.
111
APPENDIX
TABLE
2
.
112
APPENDIX
TABLE
3
.
113
REFERENCES
.
114
CURRICULUM
VITAE
.
130
ACKNOWLEDGEMENTS
.
132
STATEMENT
OF
AUTHORSHIP
.
133 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV049588607 |
| illustrated | Illustrated |
| index_date | 2024-07-03T23:33:02Z |
| indexdate | 2024-07-10T10:11:27Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-034933335 |
| oclc_num | 1371105451 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | X, 133 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| record_format | marc |
| spelling | Xu, Ying 1993- Verfasser (DE-588)1281885304 aut Study on transport mechanism of m5C-edited mRNAs von Ying Xu Potsdam November 2022 X, 133 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Die Zahl "5" im Titel ist hochgestellt Dissertation Universität Potsdam 2022 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1281851884/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034933335&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20230531 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Xu, Ying 1993- Study on transport mechanism of m5C-edited mRNAs |
| subject_GND | (DE-588)4113937-9 |
| title | Study on transport mechanism of m5C-edited mRNAs |
| title_auth | Study on transport mechanism of m5C-edited mRNAs |
| title_exact_search | Study on transport mechanism of m5C-edited mRNAs |
| title_exact_search_txtP | Study on transport mechanism of m5C-edited mRNAs |
| title_full | Study on transport mechanism of m5C-edited mRNAs von Ying Xu |
| title_fullStr | Study on transport mechanism of m5C-edited mRNAs von Ying Xu |
| title_full_unstemmed | Study on transport mechanism of m5C-edited mRNAs von Ying Xu |
| title_short | Study on transport mechanism of m5C-edited mRNAs |
| title_sort | study on transport mechanism of m5c edited mrnas |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1281851884/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034933335&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT xuying studyontransportmechanismofm5ceditedmrnas |
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