GENOME EDITING IN DRUG DISCOVERYA practical guide for researchers and professionals applying genome editing techniques to drug discoveryIn Genome Editing in Drug Discovery, a team of distinguished biologists delivers a comprehensive exploration of genome editing in the drug discovery process, with c...

Ausführliche Beschreibung

Bibliographische Detailangaben

Weitere Verfasser:	Maresca, Marcello (HerausgeberIn), Deswal, Sumit 1983- (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	Hoboken, NJ Wiley 2022
Schlagworte:	Genome Editing CRISPR/Cas-Methode Arzneimittelforschung Chemie Aufsatzsammlung
Online-Zugang:	UBR01 Volltext
Zusammenfassung:	GENOME EDITING IN DRUG DISCOVERYA practical guide for researchers and professionals applying genome editing techniques to drug discoveryIn Genome Editing in Drug Discovery, a team of distinguished biologists delivers a comprehensive exploration of genome editing in the drug discovery process, with coverage of the technology's history, current issues and techniques, and future perspectives and research directions. The book discusses techniques for disease modeling, target identification with CRISPR, safety studies, therapeutic editing, and intellectual property issues.The safety and efficacy of drugs and new target discovery, as well as next-generation therapeutics are also presented.Offering practical suggestions for practitioners and academicians involved in drug discovery, Genome Editing in Drug Discovery is a fulsome treatment of a technology that has become part of nearly every early step in the drug discovery pipeline. Selected contributions also include:* A thorough introduction to the applications of CRISPRi and CRISPRa in drug discovery* Comprehensive explorations of genome-editing applications in stem cell engineering and regenerative medicine* Practical discussions of the safety aspects of genome editing with respect to immunogenicity and the specificity of CRISPR-Cas9 gene editing* In-depth examinations of critical socio-economic and bioethical challenges in the CRISPR-Cas9 patent landscapePerfect for academic researchers and professionals in the biotech and pharmaceutical industries, Genome Editing in Drug Discovery will also earn a place in the libraries of medicinal chemists, biochemists, and molecular biologists
Beschreibung:	Prelim pages; Contents; Preface ((to follow)); 1. Genome editing in drug discovery; 2. Historical overview of Genome Editing from bacteria to higher eukaryotes; 3. CRISPR Cas: from bacterial adaptive immunity to the swiss army knife of drug discovery; 4. Commercial reagents and services for genome editing; 5. Computational tools for target design and analysis; 6. Genome editing in cellular disease models; 7. Utilizing CRISPR/Cas9 technologies for in vivo disease modeling and therapy; 8. Pooled CRISPR KO screens for target identification; 9. Functional genomics: arrayed CRISPR KO screens; 10. Applications of CRISPRi and CRISPRa in drug discovery; 11. High throughput targeted mutagenesis by CRISPR-Cas9 base editing; 12. Single-cell transcriptomics and epigenomics for CRISPR-mediated perturbation studies; 13. DNA repair pathways in the context of therapeutic genome editing; 14. DNA base editing strategies for genome editing; 15. RNA base editing technologies for gene therapy; 16. Genome Editing applications in cancer T Cell Therapy; 17. Genome-editing applications in stem cell engineering and regenerative medicine; 18. Delivery and Formulation Methods for Therapeutic Genome Editing; 19. Safety aspects of genome editing: Immunogenicity; 20. Specificity of CRISPR-Cas9 gene editing; 21. Key socio-economic and (bio)ethical challenges in the crispr-cas9 patent landscape; 22. Future prospects and emerging technologies for genome editing; Subject Index
Beschreibung:	1 Online-Ressource (x, 342 Seiten) Illustrationen, Diagramme
ISBN:	9781119671404