Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Hamburg
2021
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 74 Blätter Illustrationen, Diagramme 30 cm |
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| 245 | 1 | 0 | |a Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |c vorgelegt von: Franziska Elisabeth Marquaed |
| 264 | 1 | |a Hamburg |c 2021 | |
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Datensatz im Suchindex
| _version_ | 1804185119154503680 |
|---|---|
| adam_text | 1.
WORK
HYPOTHESIS
AND
QUESTIONING
..................................
6
2.
INTRODUCTION
.......................................................................
6
2.1
HEAD
AND
NECK
CANCER
...............................................................................................................
6
2.1.2
EPIDEMIOLOGY
AND
CHARACTERISTICS
.........................................................................................
6
2.1.3
CURRENT
THERAPY
......................................................................................................................
8
2.2
PI3K/AKT/MTOR
SIGNALING
........................................................................................................
10
2.2.1
THE
PATHWAY
........................................................................................................................
10
2.2.2
AKT
AND
ITS
ISOFORMS
............................................................................................................
11
2.2.3
MTOR
...................................................................................................................................
11
2.3
GENETIC
ALTERATIONS
IN
PI3K/AKT/MTOR
SIGNALING
FOUND
IN
HEAD
AND
NECK
CANCER
.........
12
2.4
AKT
AND
MTOR
INHIBITORS
IN
HEAD
AND
NECK
CANCER
.............................................................
13
2.4.1
AKT
INHIBITORS
.......................................................................................................................
13
2.4.2 MTOR
INHIBITORS
...................................................................................................................
14
2.4.3
COMBINED
THERAPY
................................................................................................................
15
3.
MATERIALS
AND
METHODS
...................................................
16
3.1
MATERIALS
....................................................................................................................................
16
3.1.1
MACHINES
AND
DEVICES
.........................................................................................................
16
3.1.3
BUFFER
AND
SOLUTIONS
.............................................................................................................
18
3.1.4
KITS
AND
MARKER
...................................................................................................................
21
3.1.5
ANTIBODIES
............................................................................................................................
21
3.1.7
VECTORS
................................................................................................................................
22
3.1.8
INHIBITORS
..............................................................................................................................22
3.2
CELL
LINES
.................................................................................................................................
23
3.3
SOFTWARE
.....................................................................................................................................
23
3.4
CELL
CULTURE
................................................................................................................................
23
3.4.1
DEFROSTING
CELLS
....................................................................................................................
24
3.4.2
PASSAGING
............................................................................................................................
24
3.4.3
FREEZING
CELLS
......................................................................................................................
24
3.4.3
EVALUATING
CELL
CONCENTRATIONS
.............................................................................................
24
3.5
INCUCYTE
ASSAYS
.......................................................................................................................
24
3.5.1
PROLIFERATION
.........................................................................................................................
24
3.5.2
SCRATCH
ASSAY
.....................................................................................................................
25
3.6
ALAMAR
BLUE
VIABILITY
ASSAY
.....................................................................................................
25
3
3.7
CELL
PROLIFERATION
ANALYSIS
VIA
CELL
COUNTING
...........................................................................
25
3.8
ANALYSIS
OF
APOPTOSIS
THROUGH
FLUORESCENCE
ACTIVATED
CELL
SCANNING
(FACS)
..................
25
3.9
LENTIVIRAL
KNOCKDOWN
OF
AKT
ISOFORMS
.................................................................................
26
2.9.1
TRANSFORMATION
.....................................................................................................................
26
3.9.2
PLASMID
PREPARATION
.............................................................................................................
26
3.9.3
TRANSFECTION
OFHEK293T
....................................................................................................
27
3.9.4
TRANSDUCTION
.........................................................................................................................
27
3.7
WESTERN
BLOT
.............................................................................................................................
27
3.7.1
PRODUCTION
OF
PROTEIN
LYSATES
USING
NP40
LYSIS
BUFFER
.........................................................27
3.7.2 DETERMINATION
OFTHE
PROTEIN
CONCENTRATION
USING
THE
DC
PROTEIN
ASSAY
KIT
.........................
28
3.7.3
SDS-PAGE
.............................................................................................................................
28
3.7.4
TRANSFER
AND
DEVELOPMENT
..................................................................................................
29
2.6
STATISTICAL
ANALYSIS
...................................................................................................................
29
4.
RESU
ITS
.............................................................................
30
4.1
AKT
AND
MTOR
ACTIVITY
IN
DIFFERENT
HNSCC
CELL
LINES
..........................................................
30
4.2
KNOCK
DOWN
OF
AKT
ISOFORMS
IN
UT-SCC
24A
AND
UT-SCC
24B
.........................................
30
4.2.1
EFFECT
OFAKT
ISOFORM
KNOCK
DOWN
ON
PROTEIN
EXPRESSION
AND
SIGNALING
.............................
30
4.2.2
PROLIFERATION
.........................................................................................................................
36
4.2.3
VIABILITY
................................................................................................................................
38
4.2.4
APOPTOSIS
.............................................................................................................................40
4.2.5
MIGRATION
..............................................................................................................................40
4.3
AKT
AND
MTOR
INHIBITORS
IN
UT-SCC
24A
AND
UT-SCC
24B
...............................................
41
4.3.1
PROLIFERATION
.........................................................................................................................
41
4.3.2
MIGRATION
..............................................................................................................................
44
4.3.3
PROTEIN
EXPRESSION
AND
SIGNALING
........................................................................................
47
5.
DISCUSSION
.......................................................................
50
6.
SUMMARY
..........................................................................
59
6.1
ENGLISH
.......................................................................................................................................
59
6.2
GERMAN
......................................................................................................................................
60
7.
ABBREVIATIONS
..................................................................
61
4
8.
REFERENCES
.......................................................................
62
9.
DANKSAGUNG
.....................................................................
72
10.
LEBENSLAUF
.....................................................................
72
11.
EIDESSTATTLICHE
VERSICHERUNG
.......................................
74
5
|
| adam_txt |
1.
WORK
HYPOTHESIS
AND
QUESTIONING
.
6
2.
INTRODUCTION
.
6
2.1
HEAD
AND
NECK
CANCER
.
6
2.1.2
EPIDEMIOLOGY
AND
CHARACTERISTICS
.
6
2.1.3
CURRENT
THERAPY
.
8
2.2
PI3K/AKT/MTOR
SIGNALING
.
10
2.2.1
THE
PATHWAY
.
10
2.2.2
AKT
AND
ITS
ISOFORMS
.
11
2.2.3
MTOR
.
11
2.3
GENETIC
ALTERATIONS
IN
PI3K/AKT/MTOR
SIGNALING
FOUND
IN
HEAD
AND
NECK
CANCER
.
12
2.4
AKT
AND
MTOR
INHIBITORS
IN
HEAD
AND
NECK
CANCER
.
13
2.4.1
AKT
INHIBITORS
.
13
2.4.2 MTOR
INHIBITORS
.
14
2.4.3
COMBINED
THERAPY
.
15
3.
MATERIALS
AND
METHODS
.
16
3.1
MATERIALS
.
16
3.1.1
MACHINES
AND
DEVICES
.
16
3.1.3
BUFFER
AND
SOLUTIONS
.
18
3.1.4
KITS
AND
MARKER
.
21
3.1.5
ANTIBODIES
.
21
3.1.7
VECTORS
.
22
3.1.8
INHIBITORS
.22
3.2
CELL
LINES
.
23
3.3
SOFTWARE
.
23
3.4
CELL
CULTURE
.
23
3.4.1
DEFROSTING
CELLS
.
24
3.4.2
PASSAGING
.
24
3.4.3
FREEZING
CELLS
.
24
3.4.3
EVALUATING
CELL
CONCENTRATIONS
.
24
3.5
INCUCYTE
ASSAYS
.
24
3.5.1
PROLIFERATION
.
24
3.5.2
SCRATCH
ASSAY
.
25
3.6
ALAMAR
BLUE
VIABILITY
ASSAY
.
25
3
3.7
CELL
PROLIFERATION
ANALYSIS
VIA
CELL
COUNTING
.
25
3.8
ANALYSIS
OF
APOPTOSIS
THROUGH
FLUORESCENCE
ACTIVATED
CELL
SCANNING
(FACS)
.
25
3.9
LENTIVIRAL
KNOCKDOWN
OF
AKT
ISOFORMS
.
26
2.9.1
TRANSFORMATION
.
26
3.9.2
PLASMID
PREPARATION
.
26
3.9.3
TRANSFECTION
OFHEK293T
.
27
3.9.4
TRANSDUCTION
.
27
3.7
WESTERN
BLOT
.
27
3.7.1
PRODUCTION
OF
PROTEIN
LYSATES
USING
NP40
LYSIS
BUFFER
.27
3.7.2 DETERMINATION
OFTHE
PROTEIN
CONCENTRATION
USING
THE
DC
PROTEIN
ASSAY
KIT
.
28
3.7.3
SDS-PAGE
.
28
3.7.4
TRANSFER
AND
DEVELOPMENT
.
29
2.6
STATISTICAL
ANALYSIS
.
29
4.
RESU
ITS
.
30
4.1
AKT
AND
MTOR
ACTIVITY
IN
DIFFERENT
HNSCC
CELL
LINES
.
30
4.2
KNOCK
DOWN
OF
AKT
ISOFORMS
IN
UT-SCC
24A
AND
UT-SCC
24B
.
30
4.2.1
EFFECT
OFAKT
ISOFORM
KNOCK
DOWN
ON
PROTEIN
EXPRESSION
AND
SIGNALING
.
30
4.2.2
PROLIFERATION
.
36
4.2.3
VIABILITY
.
38
4.2.4
APOPTOSIS
.40
4.2.5
MIGRATION
.40
4.3
AKT
AND
MTOR
INHIBITORS
IN
UT-SCC
24A
AND
UT-SCC
24B
.
41
4.3.1
PROLIFERATION
.
41
4.3.2
MIGRATION
.
44
4.3.3
PROTEIN
EXPRESSION
AND
SIGNALING
.
47
5.
DISCUSSION
.
50
6.
SUMMARY
.
59
6.1
ENGLISH
.
59
6.2
GERMAN
.
60
7.
ABBREVIATIONS
.
61
4
8.
REFERENCES
.
62
9.
DANKSAGUNG
.
72
10.
LEBENSLAUF
.
72
11.
EIDESSTATTLICHE
VERSICHERUNG
.
74
5 |
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| index_date | 2024-07-03T21:56:58Z |
| indexdate | 2024-07-10T09:50:10Z |
| institution | BVB |
| language | English |
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| physical | 74 Blätter Illustrationen, Diagramme 30 cm |
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| spelling | Marquard, Franziska Elisabeth Verfasser (DE-588)1271192772 aut Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer vorgelegt von: Franziska Elisabeth Marquaed Hamburg 2021 74 Blätter Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Universität Hamburg 2022 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1270544837/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034193752&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p emakn 0,33542 20221026 DE-101 https://d-nb.info/provenance/plan#emakn 2\p dnb 20221110 DE-101 https://d-nb.info/provenance/plan#dnb 3\p dnb 20221110 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Marquard, Franziska Elisabeth Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |
| subject_GND | (DE-588)4113937-9 |
| title | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |
| title_auth | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |
| title_exact_search | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |
| title_exact_search_txtP | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |
| title_full | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer vorgelegt von: Franziska Elisabeth Marquaed |
| title_fullStr | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer vorgelegt von: Franziska Elisabeth Marquaed |
| title_full_unstemmed | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer vorgelegt von: Franziska Elisabeth Marquaed |
| title_short | Investigations of PI3K/AKT/mTOR signaling and its functional role in head and neck cancer |
| title_sort | investigations of pi3k akt mtor signaling and its functional role in head and neck cancer |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1270544837/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=034193752&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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