Methods in stem cell biology: Part A
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA ; Oxford ; London
Academic Press, an imprint of Elsevier
2022
|
| Ausgabe: | First edition |
| Schriftenreihe: | Methods in cell biology
volume 170 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xvi, 210 Seiten Illustrationen, Diagramme |
| ISBN: | 9780323900201 |
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| 245 | 1 | 0 | |a Methods in stem cell biology |n Part A |c edited by Ilio Vitale (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Gwenola Manic (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Lorenzo Galluzzi (Weill Cornell Medical College, New York, NY, United States) |
| 250 | |a First edition | ||
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Datensatz im Suchindex
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| adam_text | Contents Contributors................................................................................................................... xi CHAPTER 1 Orthotopic brain tumor models derived from glioblastoma stem-like cells................ 1 Katharina Μ. Eyme, Alessandro Sammarco, and Christian E. Badr 1. Introduction......................................................................................... 2 2. Preparing a GBM stem cell line from a surgical specimen.............................................................................................. 3 3. Cell maintenance................................................................................ 5 4. Implantation of GSCs.......................................................................11 5. Monitoring of tumor growth and treatment response................... 14 Acknowledgments.................................................................................. 18 References............................................................................................... 18 CHAPTER 2 Identification and isolation of slow-cycling glioma stem cells.............................. 21 Liam Furst, Ryan J. Atkins, Marija Dinevska, Stanley S. Stylli, Niall Μ. Corcoran, Christopher Μ. Hovens, and Theo Mantamadiotis 1. Introduction..................................................................................... 22 2. Materials.......................................................................................... 25 3. Methods...... ..................................................................................... 25
4. Notes................................................................................................ 27 5. Concluding remarks........................................................................ 28 Acknowledgments................................................................. ................ 28 References............................................................................................ 29 CHAPTER 3 Immunohistochemical analysis of the developing mouse cortex................................... 31 Mohamad-Reza Aghanoori, Kaylan M.L. Burns, Maneesha Subha, Laura Williams, Michelle Hua, Farzaneh Nobakht, Taylor Krawec, and Guang Yang 1. Introduction.......................................................................................32 2. Materials and methods.................................................................... 34 v
vi Contents 3. Conclusion................................................................................... 41 4. Notes............................................................................................ 42 Acknowledgments............................................................................ 43 References.........................................................................................43 CHAPTER 4 Subcellular fractionation of brain tumor stem cells................................................ 47 Ahmad Sharanek, Laura Raco, Vahab D. Soleimani, and Arezu Jahani-Asl 1. Introduction................................................................................. 48 2. Cell maintenance......................................................................... 51 3. Subcellular fractionation..............................................................52 4. Evaluation of subcellular fraction purity by Western blotting... 53 5. Conclusion remarks..................................................................... 54 6. Notes............................................................................................ 54 Acknowledgments............................................................................ 56 References........................................................................................ 57 CHAPTER 5 microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting.................... 59 Chiara Tordonato, Matteo Jacopo Marzi, Pier Paolo Di Fiore, and Francesco Nicassio 1.
Introduction....................................................... 60 2. Materials...................................................................................... 61 3. Methods....................................................................................... 64 4. Notes............................................................................................ 76 Acknowledgments............................................................................. 79 Disclosures........................................................................................ 79 References......................................................................................... 79 CHAPTER 6 Genome-wide RNA interference screen in cancer stem cells........................................ 81 Guillaume Pinna, Marie Vandamme, Celia Rouault, Emmanuelle Charafe-Jauffret, and Christophe Ginestier 1. 2. 3. 4. Introduction.............................................................................. ...82 Global overview.......................................................................... 83 Preliminary steps.......... ..................................................... 85 Cell maintenance......................................................................... 88
Contents 5. Collagen coating of culture plates.................................................. 89 6. Reverse transfection of siRNAs......................................................90 7. ALDEFLUOR assay........................................................................ 91 8. Image acquisition and analysis....................................................... 92 9. Data pre-processing and candidate selection................................. 94 10. Notes.................................................................................................. 96 Acknowledgments....................................................................................97 Disclosures................................................................................................97 References................................................................................................ 98 CHAPTER 7 Clonal analysis of human clonogenic kératinocytes.........................................101 Elena Enzo, Camilla Cattaneo, Federica Consiglio, Maria Pia Polito, Sergio Bondanza, and Michele De Luca 1. Introduction: The meaning and importance of the clonal analysis....................................................................................... 102 2. Materials.......................................................................................... 105 3. Methods........................................................................................... 106 4. Concluding remarks........................................................................ 113
Acknowledgments................................................................................ 114 References............................................................................................. 114 CHAPTER 8 Immunofluorescence analysis of myogenic differentiation........................................ 117 Atsushi Asakura and Nobuaki Kikyo 1. Introduction..................................................................................... 118 2. Materials.......................................................................................... 119 3. Methods........................................................................................... 120 4. Immunofluorescence staining of the cells..................... 123 5. Notes................................................................................................ 124 Acknowledgments................................................................................ 124 References............................................................................................. 124 CHAPTER 9 Generating pancreatic beta-like cells from human pluripotent stem cells............. 127 Lillian Yuxian Lim, Carmen Ching, Dewei Kong, Shiao-Yng Chan, and Adrian Kee Keong Teo 1. Introduction.................................................................................... 128 2. Culture and differentiation of hPSCs...........................................129 vii
viii Contents 3. Transplantation into kidney capsule of NOD/SCID mice for in vivo maturation.......................................................................... 135 4. Glucose-stimulated insulin secretion (GSIS) assay.................... 138 5. Concluding remarks....................................................................... 140 6. Notes................................................................................................ 141 Acknowledgements..............................................................................144 Declaration of interests........................................................................145 References.............................................................................................145 CHAPTER 10 In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering............ 147 Jose Ramon Pineda, Yurena Polo, Beatriz PardoRodriguez, Jon Luzuriaga, Verónica Uribe-Etxebarria, Patricia Garcia-Gallastegui, José Ramón Sarasua, Aitor Larrañaga, and Gaskon Ibarretxe 1. Introduction..................................................................................... 148 2. Materials.................................................... 150 3. Methods........................................................................................... 153 4. Notes................................................................................................ 160 5. Concludingremarks............................................................... 164
Acknowledgments................................................................................ 165 Disclosures............................................................................................ 165 References.................................................................................... 165 CHAPTER 11 Identification of progenitor cells and their progenies in adult Drosophila midgut.169 Zhen Jin, Minhua Che, and Rongwen Xi 1. 2. 3. 4. Introduction.................................................................................... 170 Materials......................................................................................... 177 Methods.......................................................................................... 178 Assessment of proliferation and differentiation capability of ISCs.................................................................................................. 182 5. Concluding remarks...................................................................... 182 6. Notes............................................................................................... 182 Acknowledgment............................................................................... 184 References............................................................................................ 184
Contents CHAPTER 12 Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications.................................... 189 Luciana Μ. Dominguez, Esteban J. Fiore, and Guillermo D. Mazzolini 1. Introduction..................................................................................... 190 2. General considerations................................................................... 191 3. Isolation of MSCs from bone marrow..........................................192 4. Isolation of MSCs from human umbilical cord........................... 194 5. Cryopreservation and thawing MSCs........................................... 196 6. Human MSCs characterization......................................................197 7. Expected results..............................................................................200 References............................................................................................ 202 CHAPTER 13 Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells.203 Yiren Qin, Fuqiang Geng, and Duancheng Wen 1. Introduction..................................................................................... 204 2. Materials.......................................................................................... 205 3. Methods........................................................................................... 205 4. Notes................................................................................................ 207 5. Concluding
remarks....................................................................... 208 Acknowledgments................................................................................209 Disclosures............................................................................................209 References.............................................................................................210 ix
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| adam_txt |
Contents Contributors. xi CHAPTER 1 Orthotopic brain tumor models derived from glioblastoma stem-like cells. 1 Katharina Μ. Eyme, Alessandro Sammarco, and Christian E. Badr 1. Introduction. 2 2. Preparing a GBM stem cell line from a surgical specimen. 3 3. Cell maintenance. 5 4. Implantation of GSCs.11 5. Monitoring of tumor growth and treatment response. 14 Acknowledgments. 18 References. 18 CHAPTER 2 Identification and isolation of slow-cycling glioma stem cells. 21 Liam Furst, Ryan J. Atkins, Marija Dinevska, Stanley S. Stylli, Niall Μ. Corcoran, Christopher Μ. Hovens, and Theo Mantamadiotis 1. Introduction. 22 2. Materials. 25 3. Methods. . 25
4. Notes. 27 5. Concluding remarks. 28 Acknowledgments. . 28 References. 29 CHAPTER 3 Immunohistochemical analysis of the developing mouse cortex. 31 Mohamad-Reza Aghanoori, Kaylan M.L. Burns, Maneesha Subha, Laura Williams, Michelle Hua, Farzaneh Nobakht, Taylor Krawec, and Guang Yang 1. Introduction.32 2. Materials and methods. 34 v
vi Contents 3. Conclusion. 41 4. Notes. 42 Acknowledgments. 43 References.43 CHAPTER 4 Subcellular fractionation of brain tumor stem cells. 47 Ahmad Sharanek, Laura Raco, Vahab D. Soleimani, and Arezu Jahani-Asl 1. Introduction. 48 2. Cell maintenance. 51 3. Subcellular fractionation.52 4. Evaluation of subcellular fraction purity by Western blotting. 53 5. Conclusion remarks. 54 6. Notes. 54 Acknowledgments. 56 References. 57 CHAPTER 5 microRNAs transcriptional profiling of mammary stem cells isolated by PKH26 staining and FACS sorting. 59 Chiara Tordonato, Matteo Jacopo Marzi, Pier Paolo Di Fiore, and Francesco Nicassio 1.
Introduction. 60 2. Materials. 61 3. Methods. 64 4. Notes. 76 Acknowledgments. 79 Disclosures. 79 References. 79 CHAPTER 6 Genome-wide RNA interference screen in cancer stem cells. 81 Guillaume Pinna, Marie Vandamme, Celia Rouault, Emmanuelle Charafe-Jauffret, and Christophe Ginestier 1. 2. 3. 4. Introduction. .82 Global overview. 83 Preliminary steps. . 85 Cell maintenance. 88
Contents 5. Collagen coating of culture plates. 89 6. Reverse transfection of siRNAs.90 7. ALDEFLUOR assay. 91 8. Image acquisition and analysis. 92 9. Data pre-processing and candidate selection. 94 10. Notes. 96 Acknowledgments.97 Disclosures.97 References. 98 CHAPTER 7 Clonal analysis of human clonogenic kératinocytes.101 Elena Enzo, Camilla Cattaneo, Federica Consiglio, Maria Pia Polito, Sergio Bondanza, and Michele De Luca 1. Introduction: The meaning and importance of the clonal analysis. 102 2. Materials. 105 3. Methods. 106 4. Concluding remarks. 113
Acknowledgments. 114 References. 114 CHAPTER 8 Immunofluorescence analysis of myogenic differentiation. 117 Atsushi Asakura and Nobuaki Kikyo 1. Introduction. 118 2. Materials. 119 3. Methods. 120 4. Immunofluorescence staining of the cells. 123 5. Notes. 124 Acknowledgments. 124 References. 124 CHAPTER 9 Generating pancreatic beta-like cells from human pluripotent stem cells. 127 Lillian Yuxian Lim, Carmen Ching, Dewei Kong, Shiao-Yng Chan, and Adrian Kee Keong Teo 1. Introduction. 128 2. Culture and differentiation of hPSCs.129 vii
viii Contents 3. Transplantation into kidney capsule of NOD/SCID mice for in vivo maturation. 135 4. Glucose-stimulated insulin secretion (GSIS) assay. 138 5. Concluding remarks. 140 6. Notes. 141 Acknowledgements.144 Declaration of interests.145 References.145 CHAPTER 10 In vitro preparation of human Dental Pulp Stem Cell grafts with biodegradable polymer scaffolds for nerve tissue engineering. 147 Jose Ramon Pineda, Yurena Polo, Beatriz PardoRodriguez, Jon Luzuriaga, Verónica Uribe-Etxebarria, Patricia Garcia-Gallastegui, José Ramón Sarasua, Aitor Larrañaga, and Gaskon Ibarretxe 1. Introduction. 148 2. Materials. 150 3. Methods. 153 4. Notes. 160 5. Concludingremarks. 164
Acknowledgments. 165 Disclosures. 165 References. 165 CHAPTER 11 Identification of progenitor cells and their progenies in adult Drosophila midgut.169 Zhen Jin, Minhua Che, and Rongwen Xi 1. 2. 3. 4. Introduction. 170 Materials. 177 Methods. 178 Assessment of proliferation and differentiation capability of ISCs. 182 5. Concluding remarks. 182 6. Notes. 182 Acknowledgment. 184 References. 184
Contents CHAPTER 12 Generation and characterization of human mesenchymal stem/stromal cells for cell therapy applications. 189 Luciana Μ. Dominguez, Esteban J. Fiore, and Guillermo D. Mazzolini 1. Introduction. 190 2. General considerations. 191 3. Isolation of MSCs from bone marrow.192 4. Isolation of MSCs from human umbilical cord. 194 5. Cryopreservation and thawing MSCs. 196 6. Human MSCs characterization.197 7. Expected results.200 References. 202 CHAPTER 13 Generation of sex-reversed female clonal mice via CRISPR/Cas9-mediated Y chromosome deletion in male embryonic stem cells.203 Yiren Qin, Fuqiang Geng, and Duancheng Wen 1. Introduction. 204 2. Materials. 205 3. Methods. 205 4. Notes. 207 5. Concluding
remarks. 208 Acknowledgments.209 Disclosures.209 References.210 ix |
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| spelling | Methods in stem cell biology Part A edited by Ilio Vitale (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Gwenola Manic (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Lorenzo Galluzzi (Weill Cornell Medical College, New York, NY, United States) First edition Cambridge, MA ; San Diego, CA ; Oxford ; London Academic Press, an imprint of Elsevier 2022 xvi, 210 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Methods in cell biology volume 170 Methods in cell biology (DE-588)4143413-4 Aufsatzsammlung gnd-content Vitale, Ilio edt Manic, Gwenola edt Galluzzi, Lorenzo 1980- (DE-588)112867842X edt (DE-604)BV048417569 A Methods in cell biology volume 170 (DE-604)BV002534878 170 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033795932&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title_full | Methods in stem cell biology Part A edited by Ilio Vitale (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Gwenola Manic (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Lorenzo Galluzzi (Weill Cornell Medical College, New York, NY, United States) |
| title_fullStr | Methods in stem cell biology Part A edited by Ilio Vitale (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Gwenola Manic (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Lorenzo Galluzzi (Weill Cornell Medical College, New York, NY, United States) |
| title_full_unstemmed | Methods in stem cell biology Part A edited by Ilio Vitale (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Gwenola Manic (Italian Institute for Genomic Medicine, Candiolo (TO), Italy), Lorenzo Galluzzi (Weill Cornell Medical College, New York, NY, United States) |
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