The role of ubiquitin and ubiquitin-like proteins in cellular signaling:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Bonn
2021
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 106 Seiten Illustrationen 30 cm |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV048256524 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 220607s2021 gw a||| m||| 00||| eng d | ||
| 015 | |a 22,H02 |2 dnb | ||
| 016 | 7 | |a 1241060037 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1277283070 | ||
| 035 | |a (DE-599)DNB1241060037 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a 570 |2 sdnb | ||
| 084 | |8 3\p |a 570 |2 23sdnb | ||
| 084 | |8 2\p |a 610 |2 23sdnb | ||
| 084 | |a 610 |2 sdnb | ||
| 084 | |8 1\p |a 612.01 |2 23ksdnb | ||
| 100 | 1 | |a Kolbe, Carl-Christian Lothar |e Verfasser |0 (DE-588)1237651972 |4 aut | |
| 245 | 1 | 0 | |a The role of ubiquitin and ubiquitin-like proteins in cellular signaling |c vorgelegt von Carl-Christian Lothar Kolbe |
| 264 | 1 | |a Bonn |c 2021 | |
| 300 | |a 106 Seiten |b Illustrationen |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Rheinische Friedrich-Wilhelms-Universität Bonn |d 2021 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1241060037/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033636755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033636755 | ||
| 883 | 0 | |8 1\p |a aepkn |c 0,86605 |d 20220112 |q DE-101 |u https://d-nb.info/provenance/plan#aepkn | |
| 883 | 2 | |8 2\p |a dnb |d 20220113 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
| 883 | 2 | |8 3\p |a dnb |d 20220113 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
Datensatz im Suchindex
| _version_ | 1804184057330794496 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
1
ZUSAMMENFASSUNG
...................................................................................................
4
2
SUMMARY
.....................................................................................................................
5
3
INTRODUCTION
...............................................................................................................
6
3.1
IMPORTANCE
OF
POST-TRANSLATIONAL
MODIFICATIONS
................................................
6
3.2
PROTEIN
PHOSPHORYLATION
..........................................................................................
7
3.3
UBIQUITIN
AND
ITS
CODE
...........................................................................................
11
3.3.1
UBIQUITIN
..........................................................................................................
11
3.3.2
THE
CLASSICAL
UBIQUITIN
CODE
.........................................................................
14
3.3.3
THE
EXPANDED
UBIQUITIN
CODE
.......................................................................
17
3.4
UBIQUITIN-LIKE
PROTEINS
...........................................................................................
18
3.4.1
SUMO
...............................................................................................................
18
3.4.2
NEDD8
.............................................................................................................
20
3.4.3
FAT10...............................................................................................................
21
3.4.4
ISG15
...............................................................................................................
22
4
AIM
OF
THIS
THESIS
........................................................................................................
23
5
RESULTS
.........................................................................................................................
24
5.1
DEFINING
THE
UBL
LANDSCAPE
IN
HUMAN
CELLS................................................................
24
5.1.1
ANTIBODY
ARRAYS
AS
A
TOOL
TO
ELUCIDATE
UBIQUITIN
LINKAGE
TYPES
..................
30
5.2
IDENTIFICATION
OF
UBIQUITIN
KINASES
..............................................................................
33
5.2.1
LARGE
SCALE
IN
VITRO
KINASE
SCREENING
DOES
NOT
INDUCE
PHOSPHORYLATION
OF
UNTAGGED
MONO-UBIQUITIN
............................................................................................
34
5.2.2
KINASE
RECRUITMENT
BY
GST
DIMERIZATION
UNCOVERS
AGC
KINASE
MEMBERS
AS
UBIQUITIN-SPECIFIC
KINASES
......................................................................................
36
5.2.3
SERINE
57
IS
PHOSPHORYLATED
BY
PKA
AND
GST-RPS6KA5..........................
42
5.2.4
KINASE
RECRUITMENT
IS
ESSENTIAL
FOR
UBIQUITIN
PHOSPHORYLATION..................
44
5.2.5
PP1A
AND
PP2A
DEPHOSPHORYLATE
PS57-UBIQUITIN
.......................................
48
5.2.6
PROTEOMIC
INCORPORATION
OF
GST-S57-UBIQUITIN
AND
PHOSPHOMIMETIC
UBIQUITIN
MUTANTS
IN
HEK293T
CELLS
............................................................................
49
6
DISCUSSION
...............................................................................................................
52
6.1
ANTIBODY
ARRAYS
REPRESENT
A
VERSATILE
TOOL
TO
IDENTIFY
UBIQUITIN
AND
UBL
TARGETS.
52
6.2
UBIQUITIN
KINASES
REQUIRE
KINASE
RECRUITMENT
TO
INDUCE
UBIQUITIN
PHOSPHORYLATION
............................................................................................................
57
7
MATERIAL
AND
METHODS
................................................................................................
63
7.1
MATERIAL
.......................................................................................................................
63
7.2
METHODS
......................................................................................................................
71
8
LIST
OF
ABBREVIATIONS
...................................................................................................
78
9
LIST
OF
FIGURES
..............................................................................................................
80
10
LIST
OF
TABLES
...............................................................................................................
82
11
REFERENCES
...................................................................................................................
83
12
ACKNOWLEDGEMENTS
....................................................................................................
94
13
APPENDIX.....................................................................................................................
95
|
| adam_txt |
TABLE
OF
CONTENTS
1
ZUSAMMENFASSUNG
.
4
2
SUMMARY
.
5
3
INTRODUCTION
.
6
3.1
IMPORTANCE
OF
POST-TRANSLATIONAL
MODIFICATIONS
.
6
3.2
PROTEIN
PHOSPHORYLATION
.
7
3.3
UBIQUITIN
AND
ITS
CODE
.
11
3.3.1
UBIQUITIN
.
11
3.3.2
THE
CLASSICAL
UBIQUITIN
CODE
.
14
3.3.3
THE
EXPANDED
UBIQUITIN
CODE
.
17
3.4
UBIQUITIN-LIKE
PROTEINS
.
18
3.4.1
SUMO
.
18
3.4.2
NEDD8
.
20
3.4.3
FAT10.
21
3.4.4
ISG15
.
22
4
AIM
OF
THIS
THESIS
.
23
5
RESULTS
.
24
5.1
DEFINING
THE
UBL
LANDSCAPE
IN
HUMAN
CELLS.
24
5.1.1
ANTIBODY
ARRAYS
AS
A
TOOL
TO
ELUCIDATE
UBIQUITIN
LINKAGE
TYPES
.
30
5.2
IDENTIFICATION
OF
UBIQUITIN
KINASES
.
33
5.2.1
LARGE
SCALE
IN
VITRO
KINASE
SCREENING
DOES
NOT
INDUCE
PHOSPHORYLATION
OF
UNTAGGED
MONO-UBIQUITIN
.
34
5.2.2
KINASE
RECRUITMENT
BY
GST
DIMERIZATION
UNCOVERS
AGC
KINASE
MEMBERS
AS
UBIQUITIN-SPECIFIC
KINASES
.
36
5.2.3
SERINE
57
IS
PHOSPHORYLATED
BY
PKA
AND
GST-RPS6KA5.
42
5.2.4
KINASE
RECRUITMENT
IS
ESSENTIAL
FOR
UBIQUITIN
PHOSPHORYLATION.
44
5.2.5
PP1A
AND
PP2A
DEPHOSPHORYLATE
PS57-UBIQUITIN
.
48
5.2.6
PROTEOMIC
INCORPORATION
OF
GST-S57-UBIQUITIN
AND
PHOSPHOMIMETIC
UBIQUITIN
MUTANTS
IN
HEK293T
CELLS
.
49
6
DISCUSSION
.
52
6.1
ANTIBODY
ARRAYS
REPRESENT
A
VERSATILE
TOOL
TO
IDENTIFY
UBIQUITIN
AND
UBL
TARGETS.
52
6.2
UBIQUITIN
KINASES
REQUIRE
KINASE
RECRUITMENT
TO
INDUCE
UBIQUITIN
PHOSPHORYLATION
.
57
7
MATERIAL
AND
METHODS
.
63
7.1
MATERIAL
.
63
7.2
METHODS
.
71
8
LIST
OF
ABBREVIATIONS
.
78
9
LIST
OF
FIGURES
.
80
10
LIST
OF
TABLES
.
82
11
REFERENCES
.
83
12
ACKNOWLEDGEMENTS
.
94
13
APPENDIX.
95 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Kolbe, Carl-Christian Lothar |
| author_GND | (DE-588)1237651972 |
| author_facet | Kolbe, Carl-Christian Lothar |
| author_role | aut |
| author_sort | Kolbe, Carl-Christian Lothar |
| author_variant | c c l k ccl cclk |
| building | Verbundindex |
| bvnumber | BV048256524 |
| ctrlnum | (OCoLC)1277283070 (DE-599)DNB1241060037 |
| discipline | Biologie Medizin |
| discipline_str_mv | Biologie Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01767nam a2200433 c 4500</leader><controlfield tag="001">BV048256524</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">220607s2021 gw a||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">22,H02</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1241060037</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1277283070</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1241060037</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">570</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">3\p</subfield><subfield code="a">570</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">2\p</subfield><subfield code="a">610</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">610</subfield><subfield code="2">sdnb</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">1\p</subfield><subfield code="a">612.01</subfield><subfield code="2">23ksdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Kolbe, Carl-Christian Lothar</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1237651972</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The role of ubiquitin and ubiquitin-like proteins in cellular signaling</subfield><subfield code="c">vorgelegt von Carl-Christian Lothar Kolbe</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Bonn</subfield><subfield code="c">2021</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">106 Seiten</subfield><subfield code="b">Illustrationen</subfield><subfield code="c">30 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Rheinische Friedrich-Wilhelms-Universität Bonn</subfield><subfield code="d">2021</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1241060037/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033636755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033636755</subfield></datafield><datafield tag="883" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">aepkn</subfield><subfield code="c">0,86605</subfield><subfield code="d">20220112</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#aepkn</subfield></datafield><datafield tag="883" ind1="2" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">dnb</subfield><subfield code="d">20220113</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#dnb</subfield></datafield><datafield tag="883" ind1="2" ind2=" "><subfield code="8">3\p</subfield><subfield code="a">dnb</subfield><subfield code="d">20220113</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#dnb</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048256524 |
| illustrated | Illustrated |
| index_date | 2024-07-03T19:58:37Z |
| indexdate | 2024-07-10T09:33:17Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033636755 |
| oclc_num | 1277283070 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 106 Seiten Illustrationen 30 cm |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| record_format | marc |
| spelling | Kolbe, Carl-Christian Lothar Verfasser (DE-588)1237651972 aut The role of ubiquitin and ubiquitin-like proteins in cellular signaling vorgelegt von Carl-Christian Lothar Kolbe Bonn 2021 106 Seiten Illustrationen 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinische Friedrich-Wilhelms-Universität Bonn 2021 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1241060037/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033636755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p aepkn 0,86605 20220112 DE-101 https://d-nb.info/provenance/plan#aepkn 2\p dnb 20220113 DE-101 https://d-nb.info/provenance/plan#dnb 3\p dnb 20220113 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Kolbe, Carl-Christian Lothar The role of ubiquitin and ubiquitin-like proteins in cellular signaling |
| subject_GND | (DE-588)4113937-9 |
| title | The role of ubiquitin and ubiquitin-like proteins in cellular signaling |
| title_auth | The role of ubiquitin and ubiquitin-like proteins in cellular signaling |
| title_exact_search | The role of ubiquitin and ubiquitin-like proteins in cellular signaling |
| title_exact_search_txtP | The role of ubiquitin and ubiquitin-like proteins in cellular signaling |
| title_full | The role of ubiquitin and ubiquitin-like proteins in cellular signaling vorgelegt von Carl-Christian Lothar Kolbe |
| title_fullStr | The role of ubiquitin and ubiquitin-like proteins in cellular signaling vorgelegt von Carl-Christian Lothar Kolbe |
| title_full_unstemmed | The role of ubiquitin and ubiquitin-like proteins in cellular signaling vorgelegt von Carl-Christian Lothar Kolbe |
| title_short | The role of ubiquitin and ubiquitin-like proteins in cellular signaling |
| title_sort | the role of ubiquitin and ubiquitin like proteins in cellular signaling |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1241060037/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033636755&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kolbecarlchristianlothar theroleofubiquitinandubiquitinlikeproteinsincellularsignaling |
Es ist kein Print-Exemplar vorhanden.
Inhaltsverzeichnis