Engineering Pseudomonas for the production of reduced compounds:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Aachen
Apprimus Verlag
2022
|
| Edition: | 1. Auflage |
| Series: | Applied Microbiology
27 |
| Subjects: | |
| Online Access: | Volltext Inhaltsverzeichnis |
| Physical Description: | XIII, 161 Seiten Diagramme 21 cm x 14.8 cm, 258 g |
| ISBN: | 9783985550760 398555076X |
| DOI: | 10.18154/RWTH-2022-05073 |
Staff View
MARC
| LEADER | 00000nam a22000008cb4500 | ||
|---|---|---|---|
| 001 | BV048253988 | ||
| 003 | DE-604 | ||
| 005 | 20240405 | ||
| 007 | t | ||
| 008 | 220603s2022 gw |||| m||| 00||| eng d | ||
| 015 | |a 22,N20 |2 dnb | ||
| 016 | 7 | |a 1257167227 |2 DE-101 | |
| 020 | |a 9783985550760 |c : EUR 39.00 (DE), EUR 40.10 (AT) |9 978-3-98555-076-0 | ||
| 020 | |a 398555076X |9 3-98555-076-X | ||
| 024 | 3 | |a 9783985550760 | |
| 035 | |a (OCoLC)1322019310 | ||
| 035 | |a (DE-599)DNB1257167227 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-NW | ||
| 049 | |a DE-29T | ||
| 084 | |8 1\p |a 620 |2 23sdnb | ||
| 100 | 1 | |a Nies, Salome Clarissa |d 1987- |e Verfasser |0 (DE-588)1258749866 |4 aut | |
| 245 | 1 | 0 | |a Engineering Pseudomonas for the production of reduced compounds |c Salome Clarissa Nies |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2022 | |
| 300 | |a XIII, 161 Seiten |b Diagramme |c 21 cm x 14.8 cm, 258 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Applied Microbiology |v 27 | |
| 502 | |b Dissertation |c RWTH Aachen University |d 2021 | ||
| 650 | 0 | 7 | |a Methylgruppe |0 (DE-588)4169721-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Herstellung |0 (DE-588)4159653-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Stoffwechsel |0 (DE-588)4057699-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biokatalysator |0 (DE-588)4006842-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pseudomonas taiwanensis |0 (DE-588)1064240615 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fermentation |0 (DE-588)4129537-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pseudomonas |0 (DE-588)4210233-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fed-batch-Verfahren |0 (DE-588)4372478-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gentechnisch veränderter Organismus |0 (DE-588)4353579-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzymkatalyse |0 (DE-588)4152480-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ketone |0 (DE-588)4129416-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biotechnologie |0 (DE-588)4069491-4 |2 gnd |9 rswk-swf |
| 653 | |a microbes | ||
| 653 | |a microbiology | ||
| 653 | |a metabolic optimization | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Biotechnologie |0 (DE-588)4069491-4 |D s |
| 689 | 0 | 1 | |a Pseudomonas |0 (DE-588)4210233-9 |D s |
| 689 | 0 | 2 | |a Stoffwechsel |0 (DE-588)4057699-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Pseudomonas taiwanensis |0 (DE-588)1064240615 |D s |
| 689 | 1 | 1 | |a Gentechnisch veränderter Organismus |0 (DE-588)4353579-3 |D s |
| 689 | 1 | 2 | |a Biokatalysator |0 (DE-588)4006842-0 |D s |
| 689 | 1 | 3 | |a Ketone |0 (DE-588)4129416-6 |D s |
| 689 | 1 | 4 | |a Methylgruppe |0 (DE-588)4169721-2 |D s |
| 689 | 1 | 5 | |a Herstellung |0 (DE-588)4159653-5 |D s |
| 689 | 1 | 6 | |a Fermentation |0 (DE-588)4129537-7 |D s |
| 689 | 1 | 7 | |a Fed-batch-Verfahren |0 (DE-588)4372478-4 |D s |
| 689 | 1 | 8 | |a Enzymkatalyse |0 (DE-588)4152480-9 |D s |
| 689 | 1 | |5 DE-604 | |
| 710 | 2 | |a Apprimus Verlag |0 (DE-588)1068101474 |4 pbl | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe, PDF |o https://doi.org/10.18154/RWTH-2022-05073 |
| 830 | 0 | |a Applied Microbiology |v 27 |w (DE-604)BV042644516 |9 27 | |
| 856 | 4 | 1 | |u https://doi.org/10.18154/RWTH-2022-05073 |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033634264&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033634264 | ||
| 883 | 1 | |8 1\p |a vlb |d 20220510 |q DE-101 |u https://d-nb.info/provenance/plan#vlb | |
Record in the Search Index
| _version_ | 1804184053129150464 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
SUMMARY
.......................................................................................................................................
V
ZUSAMMENFASSUNG
.....................................................................................................................VII
LIST
OF
ABBREVIATIONS
...................................................................................................................
,X
LIST
OF
FIGURES
................................................................................................................................
XL
LIST
OF
TABLES
...............................................................................................................................
XL11
1
GENERAL
INTRODUCTION
............................................................................................................
3
1.1
THE
BIO-ECONOMY
IS
AN
ATTRACTIVE
ALTERNATIVE
TO
THE
PETROCHEMICAL-BASED
INDUSTRY
3
1.2
CHALLENGES
OF
THE
PRODUCTION
OF
REDUCED
COMPOUNDS
...............................................
3
1.3
STRATEGY
DESIGN
TO
OVERCOME
REDOX
LIMITATION
.............................................................
5
1.4
DEVELOPMENT
OF
MICROBIAL
CELL
FACTORIES
APPLYING
METABOLIC
ENGINEERING
.................
7
1.5
MEDIUM-CHAIN
METHYL
KETONES
C11-C17
AS
THE
TARGET
PRODUCT
OF
THIS
STUDY
...........
9
1.6
APPLIED
STRATEGIES
FOR
THE
PRODUCTION
OF
METHYL
KETONES
IN
DIFFERENT
SPECIES........
11
1.7
SELECTION
OF
THE
PRODUCTION
HOST:
PSEUDOMONAS
AS
A
SUITABLE
PRODUCTION
PLATFORM
16
1.8
SCOPE
OF
THIS
THESIS
......................................................................................................
20
2
MATERIAL
AND
METHODS
...........................................................................................................
23
2.1
BACTERIAL
STRAINS
USED
IN
THIS
STUDY
..............................................................................
23
2.2
PRIMER
USED
IN
THIS
STUDY
..............................................................................................
28
2.4
PLASMID
AND
STRAIN
CONSTRUCTION,
GENOMIC
MODIFICATIONS
..........................................
33
2.5
ANALYSIS
OF
CELL
GROWTH
AND
SUGAR
METABOLISM
DURING
AQUEOUS
ORGANIC
TWO-PHASE
CULTIVATIONS
..............................................................................................................................
34
2.6
METHYL
KETONE
ANALYSIS
WITH
GAS
CHROMATOGRAPHY
.....................................................
35
2.7
PROTEOMIC
PROFILING
OF
NADH
DEHYDROGENASE
MUTANTS
..............................................
37
2.8
RNA
PREPARATION
AND
ANALYSIS
......................................................................................
38
2.9
INVERTED
MEMBRANE
VESICLE
PREPARATION
AND
NADH
OXIDATION
ACTIVITY
.....................
39
2.10
RESPIRATION
ACTIVITY
MONITORING
....................................................................................
40
2.11
REDOX
COFACTOR
QUANTIFICATION
......................................................................................
40
TABLE
OF
CONTENTS
2.12
ROS
ASSAY
......................................................................................................................
41
2.13
PHA
QUANTIFICATION
WITH
GAS
CHROMATOGRAPHY
...........................................................
41
2.14
CULTIVATION
AND
NON-INVASIVE
ONLINE
MONITORING
USING
BIOLECTOR
.............................
42
2.15
CALCULATION
OF
DERIVED
CETANE
NUMBER
.......................................................................
43
2.16
BIOREACTOR
CULTIVATIONS
FOR
METHYL
KETONE
PRODUCTION
.............................................
43
2.17
MODEL-GUIDED
STRAIN-DESIGN
........................................................................................
43
3
RESULTS..................................................................................................................................
47
3.1
THE
METABOLIC
RESPONSE
OF
PSEUDOMONAS
TAIWANENSIS
TO
NADH
DEHYDROGENASE
DEFICIENCY
.................................................................................................................................
50
3.1.1
ABSTRACT
...................................................................................................................
50
3.1.2
INTRODUCTION
.............................................................................................................
50
3.1.3
RESULTS
.....................................................................................................................
52
3.1.4
DISCUSSION
................................................................................................................
66
3.1.5
CONCLUSION
...............................................................................................................
69
3.2
A
BASIC
DESIGN
OF
A
PSEUDOMONAS
CHASSIS
FOR
REDOX-COFACTOR
DEPENDENT
BIOCATALYSIS
73
3.2.1
ABSTRACT
...................................................................................................................
73
3.2.2
INTRODUCTION
.............................................................................................................
73
3.2.3
DISCUSSION
................................................................................................................
89
3.2.4
CONCLUSION
...............................................................................................................
91
3.3
HIGH
TITER
METHYL
KETONE
PRODUCTION
WITH
TAILORED
PSEUDOMONAS
TAIWANENSIS
V
LB120
95
3.3.1
ABSTRACT
...................................................................................................................
95
3.3.2
INTRODUCTION
.............................................................................................................
95
3.3.3
RESULTS
AND
DISCUSSION
............................................................................................
97
3.3.4
CONCLUSION
.............................................................................................................
110
3.4
METHYL
KETONE
PRODUCTION
IN
THE
PSEUDOMONAS
REDOX
CHASSIS..............................
115
3.4.1
ABSTRACT
.................................................................................................................
115
3.4.2
INTRODUCTION
...........................................................................................................
115
3.4.3
RESULTS
AND
DISCUSSION
...........................................................................................
116
3.4.4
CONCLUSION
.............................................................................................................
121
4
GENERAL
DISCUSSION
AND
OUTLOOK
.......................................................................................
125
II
TABLE
OF
CONTENTS
4.1
HURDLES
AND
CHALLENGES
OF
CHASSIS
DEVELOPMENT
TO
PROVIDE
DESIRABLE
FUNCTIONS
AND
ENHANCED
PERFORMANCE
.........................................................................................................
125
4.2
OPTIMIZATION
OF
METABOLIC
PATHWAY
RECONSTRUCTION
................................................
127
4.3
OPTIMIZATION
OF
BIOPROCESS
PARAMETERS
..................................................................
127
4.4
EXPANDING
THE
METHYL
KETONE
PORTFOLIO
....................................................................
129
4.5
CONCLUSION
..................................................................................................................
130
5
APPENDIX.............................................................................................................................
133
6
REFERENCES..........................................................................................................................
137
7
CURRICULUM
VITAE
.................................................................................................................
159
III
|
| adam_txt |
TABLE
OF
CONTENTS
SUMMARY
.
V
ZUSAMMENFASSUNG
.VII
LIST
OF
ABBREVIATIONS
.
,X
LIST
OF
FIGURES
.
XL
LIST
OF
TABLES
.
XL11
1
GENERAL
INTRODUCTION
.
3
1.1
THE
BIO-ECONOMY
IS
AN
ATTRACTIVE
ALTERNATIVE
TO
THE
PETROCHEMICAL-BASED
INDUSTRY
3
1.2
CHALLENGES
OF
THE
PRODUCTION
OF
REDUCED
COMPOUNDS
.
3
1.3
STRATEGY
DESIGN
TO
OVERCOME
REDOX
LIMITATION
.
5
1.4
DEVELOPMENT
OF
MICROBIAL
CELL
FACTORIES
APPLYING
METABOLIC
ENGINEERING
.
7
1.5
MEDIUM-CHAIN
METHYL
KETONES
C11-C17
AS
THE
TARGET
PRODUCT
OF
THIS
STUDY
.
9
1.6
APPLIED
STRATEGIES
FOR
THE
PRODUCTION
OF
METHYL
KETONES
IN
DIFFERENT
SPECIES.
11
1.7
SELECTION
OF
THE
PRODUCTION
HOST:
PSEUDOMONAS
AS
A
SUITABLE
PRODUCTION
PLATFORM
16
1.8
SCOPE
OF
THIS
THESIS
.
20
2
MATERIAL
AND
METHODS
.
23
2.1
BACTERIAL
STRAINS
USED
IN
THIS
STUDY
.
23
2.2
PRIMER
USED
IN
THIS
STUDY
.
28
2.4
PLASMID
AND
STRAIN
CONSTRUCTION,
GENOMIC
MODIFICATIONS
.
33
2.5
ANALYSIS
OF
CELL
GROWTH
AND
SUGAR
METABOLISM
DURING
AQUEOUS
ORGANIC
TWO-PHASE
CULTIVATIONS
.
34
2.6
METHYL
KETONE
ANALYSIS
WITH
GAS
CHROMATOGRAPHY
.
35
2.7
PROTEOMIC
PROFILING
OF
NADH
DEHYDROGENASE
MUTANTS
.
37
2.8
RNA
PREPARATION
AND
ANALYSIS
.
38
2.9
INVERTED
MEMBRANE
VESICLE
PREPARATION
AND
NADH
OXIDATION
ACTIVITY
.
39
2.10
RESPIRATION
ACTIVITY
MONITORING
.
40
2.11
REDOX
COFACTOR
QUANTIFICATION
.
40
TABLE
OF
CONTENTS
2.12
ROS
ASSAY
.
41
2.13
PHA
QUANTIFICATION
WITH
GAS
CHROMATOGRAPHY
.
41
2.14
CULTIVATION
AND
NON-INVASIVE
ONLINE
MONITORING
USING
BIOLECTOR
.
42
2.15
CALCULATION
OF
DERIVED
CETANE
NUMBER
.
43
2.16
BIOREACTOR
CULTIVATIONS
FOR
METHYL
KETONE
PRODUCTION
.
43
2.17
MODEL-GUIDED
STRAIN-DESIGN
.
43
3
RESULTS.
47
3.1
THE
METABOLIC
RESPONSE
OF
PSEUDOMONAS
TAIWANENSIS
TO
NADH
DEHYDROGENASE
DEFICIENCY
.
50
3.1.1
ABSTRACT
.
50
3.1.2
INTRODUCTION
.
50
3.1.3
RESULTS
.
52
3.1.4
DISCUSSION
.
66
3.1.5
CONCLUSION
.
69
3.2
A
BASIC
DESIGN
OF
A
PSEUDOMONAS
CHASSIS
FOR
REDOX-COFACTOR
DEPENDENT
BIOCATALYSIS
73
3.2.1
ABSTRACT
.
73
3.2.2
INTRODUCTION
.
73
3.2.3
DISCUSSION
.
89
3.2.4
CONCLUSION
.
91
3.3
HIGH
TITER
METHYL
KETONE
PRODUCTION
WITH
TAILORED
PSEUDOMONAS
TAIWANENSIS
V
LB120
95
3.3.1
ABSTRACT
.
95
3.3.2
INTRODUCTION
.
95
3.3.3
RESULTS
AND
DISCUSSION
.
97
3.3.4
CONCLUSION
.
110
3.4
METHYL
KETONE
PRODUCTION
IN
THE
PSEUDOMONAS
REDOX
CHASSIS.
115
3.4.1
ABSTRACT
.
115
3.4.2
INTRODUCTION
.
115
3.4.3
RESULTS
AND
DISCUSSION
.
116
3.4.4
CONCLUSION
.
121
4
GENERAL
DISCUSSION
AND
OUTLOOK
.
125
II
TABLE
OF
CONTENTS
4.1
HURDLES
AND
CHALLENGES
OF
CHASSIS
DEVELOPMENT
TO
PROVIDE
DESIRABLE
FUNCTIONS
AND
ENHANCED
PERFORMANCE
.
125
4.2
OPTIMIZATION
OF
METABOLIC
PATHWAY
RECONSTRUCTION
.
127
4.3
OPTIMIZATION
OF
BIOPROCESS
PARAMETERS
.
127
4.4
EXPANDING
THE
METHYL
KETONE
PORTFOLIO
.
129
4.5
CONCLUSION
.
130
5
APPENDIX.
133
6
REFERENCES.
137
7
CURRICULUM
VITAE
.
159
III |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Nies, Salome Clarissa 1987- |
| author_GND | (DE-588)1258749866 |
| author_facet | Nies, Salome Clarissa 1987- |
| author_role | aut |
| author_sort | Nies, Salome Clarissa 1987- |
| author_variant | s c n sc scn |
| building | Verbundindex |
| bvnumber | BV048253988 |
| collection | ebook |
| ctrlnum | (OCoLC)1322019310 (DE-599)DNB1257167227 |
| doi_str_mv | 10.18154/RWTH-2022-05073 |
| edition | 1. Auflage |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03480nam a22008058cb4500</leader><controlfield tag="001">BV048253988</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20240405 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">220603s2022 gw |||| m||| 00||| eng d</controlfield><datafield tag="015" ind1=" " ind2=" "><subfield code="a">22,N20</subfield><subfield code="2">dnb</subfield></datafield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1257167227</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9783985550760</subfield><subfield code="c">: EUR 39.00 (DE), EUR 40.10 (AT)</subfield><subfield code="9">978-3-98555-076-0</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">398555076X</subfield><subfield code="9">3-98555-076-X</subfield></datafield><datafield tag="024" ind1="3" ind2=" "><subfield code="a">9783985550760</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1322019310</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1257167227</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE-NW</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29T</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="8">1\p</subfield><subfield code="a">620</subfield><subfield code="2">23sdnb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Nies, Salome Clarissa</subfield><subfield code="d">1987-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1258749866</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Engineering Pseudomonas for the production of reduced compounds</subfield><subfield code="c">Salome Clarissa Nies</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. Auflage</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Aachen</subfield><subfield code="b">Apprimus Verlag</subfield><subfield code="c">2022</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XIII, 161 Seiten</subfield><subfield code="b">Diagramme</subfield><subfield code="c">21 cm x 14.8 cm, 258 g</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Applied Microbiology</subfield><subfield code="v">27</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">RWTH Aachen University</subfield><subfield code="d">2021</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methylgruppe</subfield><subfield code="0">(DE-588)4169721-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Herstellung</subfield><subfield code="0">(DE-588)4159653-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Stoffwechsel</subfield><subfield code="0">(DE-588)4057699-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biokatalysator</subfield><subfield code="0">(DE-588)4006842-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Pseudomonas taiwanensis</subfield><subfield code="0">(DE-588)1064240615</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fermentation</subfield><subfield code="0">(DE-588)4129537-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Pseudomonas</subfield><subfield code="0">(DE-588)4210233-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Fed-batch-Verfahren</subfield><subfield code="0">(DE-588)4372478-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Gentechnisch veränderter Organismus</subfield><subfield code="0">(DE-588)4353579-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Enzymkatalyse</subfield><subfield code="0">(DE-588)4152480-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Ketone</subfield><subfield code="0">(DE-588)4129416-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biotechnologie</subfield><subfield code="0">(DE-588)4069491-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">microbes</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">microbiology</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">metabolic optimization</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Biotechnologie</subfield><subfield code="0">(DE-588)4069491-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Pseudomonas</subfield><subfield code="0">(DE-588)4210233-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Stoffwechsel</subfield><subfield code="0">(DE-588)4057699-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Pseudomonas taiwanensis</subfield><subfield code="0">(DE-588)1064240615</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Gentechnisch veränderter Organismus</subfield><subfield code="0">(DE-588)4353579-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="2"><subfield code="a">Biokatalysator</subfield><subfield code="0">(DE-588)4006842-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="3"><subfield code="a">Ketone</subfield><subfield code="0">(DE-588)4129416-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="4"><subfield code="a">Methylgruppe</subfield><subfield code="0">(DE-588)4169721-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="5"><subfield code="a">Herstellung</subfield><subfield code="0">(DE-588)4159653-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="6"><subfield code="a">Fermentation</subfield><subfield code="0">(DE-588)4129537-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="7"><subfield code="a">Fed-batch-Verfahren</subfield><subfield code="0">(DE-588)4372478-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="8"><subfield code="a">Enzymkatalyse</subfield><subfield code="0">(DE-588)4152480-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="710" ind1="2" ind2=" "><subfield code="a">Apprimus Verlag</subfield><subfield code="0">(DE-588)1068101474</subfield><subfield code="4">pbl</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe, PDF</subfield><subfield code="o">https://doi.org/10.18154/RWTH-2022-05073</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Applied Microbiology</subfield><subfield code="v">27</subfield><subfield code="w">(DE-604)BV042644516</subfield><subfield code="9">27</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.18154/RWTH-2022-05073</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033634264&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ebook</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033634264</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">vlb</subfield><subfield code="d">20220510</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#vlb</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048253988 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T19:57:45Z |
| indexdate | 2024-07-10T09:33:13Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783985550760 398555076X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033634264 |
| oclc_num | 1322019310 |
| open_access_boolean | 1 |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XIII, 161 Seiten Diagramme 21 cm x 14.8 cm, 258 g |
| psigel | ebook |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied Microbiology |
| series2 | Applied Microbiology |
| spelling | Nies, Salome Clarissa 1987- Verfasser (DE-588)1258749866 aut Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies 1. Auflage Aachen Apprimus Verlag 2022 XIII, 161 Seiten Diagramme 21 cm x 14.8 cm, 258 g txt rdacontent n rdamedia nc rdacarrier Applied Microbiology 27 Dissertation RWTH Aachen University 2021 Methylgruppe (DE-588)4169721-2 gnd rswk-swf Herstellung (DE-588)4159653-5 gnd rswk-swf Stoffwechsel (DE-588)4057699-1 gnd rswk-swf Biokatalysator (DE-588)4006842-0 gnd rswk-swf Pseudomonas taiwanensis (DE-588)1064240615 gnd rswk-swf Fermentation (DE-588)4129537-7 gnd rswk-swf Pseudomonas (DE-588)4210233-9 gnd rswk-swf Fed-batch-Verfahren (DE-588)4372478-4 gnd rswk-swf Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Ketone (DE-588)4129416-6 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf microbes microbiology metabolic optimization (DE-588)4113937-9 Hochschulschrift gnd-content Biotechnologie (DE-588)4069491-4 s Pseudomonas (DE-588)4210233-9 s Stoffwechsel (DE-588)4057699-1 s DE-604 Pseudomonas taiwanensis (DE-588)1064240615 s Gentechnisch veränderter Organismus (DE-588)4353579-3 s Biokatalysator (DE-588)4006842-0 s Ketone (DE-588)4129416-6 s Methylgruppe (DE-588)4169721-2 s Herstellung (DE-588)4159653-5 s Fermentation (DE-588)4129537-7 s Fed-batch-Verfahren (DE-588)4372478-4 s Enzymkatalyse (DE-588)4152480-9 s Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2022-05073 Applied Microbiology 27 (DE-604)BV042644516 27 https://doi.org/10.18154/RWTH-2022-05073 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033634264&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p vlb 20220510 DE-101 https://d-nb.info/provenance/plan#vlb |
| spellingShingle | Nies, Salome Clarissa 1987- Engineering Pseudomonas for the production of reduced compounds Applied Microbiology Methylgruppe (DE-588)4169721-2 gnd Herstellung (DE-588)4159653-5 gnd Stoffwechsel (DE-588)4057699-1 gnd Biokatalysator (DE-588)4006842-0 gnd Pseudomonas taiwanensis (DE-588)1064240615 gnd Fermentation (DE-588)4129537-7 gnd Pseudomonas (DE-588)4210233-9 gnd Fed-batch-Verfahren (DE-588)4372478-4 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Enzymkatalyse (DE-588)4152480-9 gnd Ketone (DE-588)4129416-6 gnd Biotechnologie (DE-588)4069491-4 gnd |
| subject_GND | (DE-588)4169721-2 (DE-588)4159653-5 (DE-588)4057699-1 (DE-588)4006842-0 (DE-588)1064240615 (DE-588)4129537-7 (DE-588)4210233-9 (DE-588)4372478-4 (DE-588)4353579-3 (DE-588)4152480-9 (DE-588)4129416-6 (DE-588)4069491-4 (DE-588)4113937-9 |
| title | Engineering Pseudomonas for the production of reduced compounds |
| title_auth | Engineering Pseudomonas for the production of reduced compounds |
| title_exact_search | Engineering Pseudomonas for the production of reduced compounds |
| title_exact_search_txtP | Engineering Pseudomonas for the production of reduced compounds |
| title_full | Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies |
| title_fullStr | Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies |
| title_full_unstemmed | Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies |
| title_short | Engineering Pseudomonas for the production of reduced compounds |
| title_sort | engineering pseudomonas for the production of reduced compounds |
| topic | Methylgruppe (DE-588)4169721-2 gnd Herstellung (DE-588)4159653-5 gnd Stoffwechsel (DE-588)4057699-1 gnd Biokatalysator (DE-588)4006842-0 gnd Pseudomonas taiwanensis (DE-588)1064240615 gnd Fermentation (DE-588)4129537-7 gnd Pseudomonas (DE-588)4210233-9 gnd Fed-batch-Verfahren (DE-588)4372478-4 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Enzymkatalyse (DE-588)4152480-9 gnd Ketone (DE-588)4129416-6 gnd Biotechnologie (DE-588)4069491-4 gnd |
| topic_facet | Methylgruppe Herstellung Stoffwechsel Biokatalysator Pseudomonas taiwanensis Fermentation Pseudomonas Fed-batch-Verfahren Gentechnisch veränderter Organismus Enzymkatalyse Ketone Biotechnologie Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2022-05073 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033634264&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT niessalomeclarissa engineeringpseudomonasfortheproductionofreducedcompounds AT apprimusverlag engineeringpseudomonasfortheproductionofreducedcompounds |