Engineering Pseudomonas for the production of reduced compounds:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
Apprimus Verlag
2022
|
| Ausgabe: | 1. Auflage |
| Schriftenreihe: | Applied Microbiology
27 |
| Schlagworte: | |
| Online-Zugang: | Volltext Inhaltsverzeichnis |
| Beschreibung: | XIII, 161 Seiten Diagramme 21 cm x 14.8 cm, 258 g |
| ISBN: | 9783985550760 398555076X |
| DOI: | 10.18154/RWTH-2022-05073 |
Internformat
MARC
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| 100 | 1 | |a Nies, Salome Clarissa |d 1987- |e Verfasser |0 (DE-588)1258749866 |4 aut | |
| 245 | 1 | 0 | |a Engineering Pseudomonas for the production of reduced compounds |c Salome Clarissa Nies |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Aachen |b Apprimus Verlag |c 2022 | |
| 300 | |a XIII, 161 Seiten |b Diagramme |c 21 cm x 14.8 cm, 258 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Applied Microbiology |v 27 | |
| 502 | |b Dissertation |c RWTH Aachen University |d 2021 | ||
| 650 | 0 | 7 | |a Methylgruppe |0 (DE-588)4169721-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Herstellung |0 (DE-588)4159653-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Stoffwechsel |0 (DE-588)4057699-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biokatalysator |0 (DE-588)4006842-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pseudomonas taiwanensis |0 (DE-588)1064240615 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fermentation |0 (DE-588)4129537-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pseudomonas |0 (DE-588)4210233-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Fed-batch-Verfahren |0 (DE-588)4372478-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Gentechnisch veränderter Organismus |0 (DE-588)4353579-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzymkatalyse |0 (DE-588)4152480-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ketone |0 (DE-588)4129416-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biotechnologie |0 (DE-588)4069491-4 |2 gnd |9 rswk-swf |
| 653 | |a microbes | ||
| 653 | |a microbiology | ||
| 653 | |a metabolic optimization | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Biotechnologie |0 (DE-588)4069491-4 |D s |
| 689 | 0 | 1 | |a Pseudomonas |0 (DE-588)4210233-9 |D s |
| 689 | 0 | 2 | |a Stoffwechsel |0 (DE-588)4057699-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Pseudomonas taiwanensis |0 (DE-588)1064240615 |D s |
| 689 | 1 | 1 | |a Gentechnisch veränderter Organismus |0 (DE-588)4353579-3 |D s |
| 689 | 1 | 2 | |a Biokatalysator |0 (DE-588)4006842-0 |D s |
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Datensatz im Suchindex
| _version_ | 1804184053129150464 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
SUMMARY
.......................................................................................................................................
V
ZUSAMMENFASSUNG
.....................................................................................................................VII
LIST
OF
ABBREVIATIONS
...................................................................................................................
,X
LIST
OF
FIGURES
................................................................................................................................
XL
LIST
OF
TABLES
...............................................................................................................................
XL11
1
GENERAL
INTRODUCTION
............................................................................................................
3
1.1
THE
BIO-ECONOMY
IS
AN
ATTRACTIVE
ALTERNATIVE
TO
THE
PETROCHEMICAL-BASED
INDUSTRY
3
1.2
CHALLENGES
OF
THE
PRODUCTION
OF
REDUCED
COMPOUNDS
...............................................
3
1.3
STRATEGY
DESIGN
TO
OVERCOME
REDOX
LIMITATION
.............................................................
5
1.4
DEVELOPMENT
OF
MICROBIAL
CELL
FACTORIES
APPLYING
METABOLIC
ENGINEERING
.................
7
1.5
MEDIUM-CHAIN
METHYL
KETONES
C11-C17
AS
THE
TARGET
PRODUCT
OF
THIS
STUDY
...........
9
1.6
APPLIED
STRATEGIES
FOR
THE
PRODUCTION
OF
METHYL
KETONES
IN
DIFFERENT
SPECIES........
11
1.7
SELECTION
OF
THE
PRODUCTION
HOST:
PSEUDOMONAS
AS
A
SUITABLE
PRODUCTION
PLATFORM
16
1.8
SCOPE
OF
THIS
THESIS
......................................................................................................
20
2
MATERIAL
AND
METHODS
...........................................................................................................
23
2.1
BACTERIAL
STRAINS
USED
IN
THIS
STUDY
..............................................................................
23
2.2
PRIMER
USED
IN
THIS
STUDY
..............................................................................................
28
2.4
PLASMID
AND
STRAIN
CONSTRUCTION,
GENOMIC
MODIFICATIONS
..........................................
33
2.5
ANALYSIS
OF
CELL
GROWTH
AND
SUGAR
METABOLISM
DURING
AQUEOUS
ORGANIC
TWO-PHASE
CULTIVATIONS
..............................................................................................................................
34
2.6
METHYL
KETONE
ANALYSIS
WITH
GAS
CHROMATOGRAPHY
.....................................................
35
2.7
PROTEOMIC
PROFILING
OF
NADH
DEHYDROGENASE
MUTANTS
..............................................
37
2.8
RNA
PREPARATION
AND
ANALYSIS
......................................................................................
38
2.9
INVERTED
MEMBRANE
VESICLE
PREPARATION
AND
NADH
OXIDATION
ACTIVITY
.....................
39
2.10
RESPIRATION
ACTIVITY
MONITORING
....................................................................................
40
2.11
REDOX
COFACTOR
QUANTIFICATION
......................................................................................
40
TABLE
OF
CONTENTS
2.12
ROS
ASSAY
......................................................................................................................
41
2.13
PHA
QUANTIFICATION
WITH
GAS
CHROMATOGRAPHY
...........................................................
41
2.14
CULTIVATION
AND
NON-INVASIVE
ONLINE
MONITORING
USING
BIOLECTOR
.............................
42
2.15
CALCULATION
OF
DERIVED
CETANE
NUMBER
.......................................................................
43
2.16
BIOREACTOR
CULTIVATIONS
FOR
METHYL
KETONE
PRODUCTION
.............................................
43
2.17
MODEL-GUIDED
STRAIN-DESIGN
........................................................................................
43
3
RESULTS..................................................................................................................................
47
3.1
THE
METABOLIC
RESPONSE
OF
PSEUDOMONAS
TAIWANENSIS
TO
NADH
DEHYDROGENASE
DEFICIENCY
.................................................................................................................................
50
3.1.1
ABSTRACT
...................................................................................................................
50
3.1.2
INTRODUCTION
.............................................................................................................
50
3.1.3
RESULTS
.....................................................................................................................
52
3.1.4
DISCUSSION
................................................................................................................
66
3.1.5
CONCLUSION
...............................................................................................................
69
3.2
A
BASIC
DESIGN
OF
A
PSEUDOMONAS
CHASSIS
FOR
REDOX-COFACTOR
DEPENDENT
BIOCATALYSIS
73
3.2.1
ABSTRACT
...................................................................................................................
73
3.2.2
INTRODUCTION
.............................................................................................................
73
3.2.3
DISCUSSION
................................................................................................................
89
3.2.4
CONCLUSION
...............................................................................................................
91
3.3
HIGH
TITER
METHYL
KETONE
PRODUCTION
WITH
TAILORED
PSEUDOMONAS
TAIWANENSIS
V
LB120
95
3.3.1
ABSTRACT
...................................................................................................................
95
3.3.2
INTRODUCTION
.............................................................................................................
95
3.3.3
RESULTS
AND
DISCUSSION
............................................................................................
97
3.3.4
CONCLUSION
.............................................................................................................
110
3.4
METHYL
KETONE
PRODUCTION
IN
THE
PSEUDOMONAS
REDOX
CHASSIS..............................
115
3.4.1
ABSTRACT
.................................................................................................................
115
3.4.2
INTRODUCTION
...........................................................................................................
115
3.4.3
RESULTS
AND
DISCUSSION
...........................................................................................
116
3.4.4
CONCLUSION
.............................................................................................................
121
4
GENERAL
DISCUSSION
AND
OUTLOOK
.......................................................................................
125
II
TABLE
OF
CONTENTS
4.1
HURDLES
AND
CHALLENGES
OF
CHASSIS
DEVELOPMENT
TO
PROVIDE
DESIRABLE
FUNCTIONS
AND
ENHANCED
PERFORMANCE
.........................................................................................................
125
4.2
OPTIMIZATION
OF
METABOLIC
PATHWAY
RECONSTRUCTION
................................................
127
4.3
OPTIMIZATION
OF
BIOPROCESS
PARAMETERS
..................................................................
127
4.4
EXPANDING
THE
METHYL
KETONE
PORTFOLIO
....................................................................
129
4.5
CONCLUSION
..................................................................................................................
130
5
APPENDIX.............................................................................................................................
133
6
REFERENCES..........................................................................................................................
137
7
CURRICULUM
VITAE
.................................................................................................................
159
III
|
| adam_txt |
TABLE
OF
CONTENTS
SUMMARY
.
V
ZUSAMMENFASSUNG
.VII
LIST
OF
ABBREVIATIONS
.
,X
LIST
OF
FIGURES
.
XL
LIST
OF
TABLES
.
XL11
1
GENERAL
INTRODUCTION
.
3
1.1
THE
BIO-ECONOMY
IS
AN
ATTRACTIVE
ALTERNATIVE
TO
THE
PETROCHEMICAL-BASED
INDUSTRY
3
1.2
CHALLENGES
OF
THE
PRODUCTION
OF
REDUCED
COMPOUNDS
.
3
1.3
STRATEGY
DESIGN
TO
OVERCOME
REDOX
LIMITATION
.
5
1.4
DEVELOPMENT
OF
MICROBIAL
CELL
FACTORIES
APPLYING
METABOLIC
ENGINEERING
.
7
1.5
MEDIUM-CHAIN
METHYL
KETONES
C11-C17
AS
THE
TARGET
PRODUCT
OF
THIS
STUDY
.
9
1.6
APPLIED
STRATEGIES
FOR
THE
PRODUCTION
OF
METHYL
KETONES
IN
DIFFERENT
SPECIES.
11
1.7
SELECTION
OF
THE
PRODUCTION
HOST:
PSEUDOMONAS
AS
A
SUITABLE
PRODUCTION
PLATFORM
16
1.8
SCOPE
OF
THIS
THESIS
.
20
2
MATERIAL
AND
METHODS
.
23
2.1
BACTERIAL
STRAINS
USED
IN
THIS
STUDY
.
23
2.2
PRIMER
USED
IN
THIS
STUDY
.
28
2.4
PLASMID
AND
STRAIN
CONSTRUCTION,
GENOMIC
MODIFICATIONS
.
33
2.5
ANALYSIS
OF
CELL
GROWTH
AND
SUGAR
METABOLISM
DURING
AQUEOUS
ORGANIC
TWO-PHASE
CULTIVATIONS
.
34
2.6
METHYL
KETONE
ANALYSIS
WITH
GAS
CHROMATOGRAPHY
.
35
2.7
PROTEOMIC
PROFILING
OF
NADH
DEHYDROGENASE
MUTANTS
.
37
2.8
RNA
PREPARATION
AND
ANALYSIS
.
38
2.9
INVERTED
MEMBRANE
VESICLE
PREPARATION
AND
NADH
OXIDATION
ACTIVITY
.
39
2.10
RESPIRATION
ACTIVITY
MONITORING
.
40
2.11
REDOX
COFACTOR
QUANTIFICATION
.
40
TABLE
OF
CONTENTS
2.12
ROS
ASSAY
.
41
2.13
PHA
QUANTIFICATION
WITH
GAS
CHROMATOGRAPHY
.
41
2.14
CULTIVATION
AND
NON-INVASIVE
ONLINE
MONITORING
USING
BIOLECTOR
.
42
2.15
CALCULATION
OF
DERIVED
CETANE
NUMBER
.
43
2.16
BIOREACTOR
CULTIVATIONS
FOR
METHYL
KETONE
PRODUCTION
.
43
2.17
MODEL-GUIDED
STRAIN-DESIGN
.
43
3
RESULTS.
47
3.1
THE
METABOLIC
RESPONSE
OF
PSEUDOMONAS
TAIWANENSIS
TO
NADH
DEHYDROGENASE
DEFICIENCY
.
50
3.1.1
ABSTRACT
.
50
3.1.2
INTRODUCTION
.
50
3.1.3
RESULTS
.
52
3.1.4
DISCUSSION
.
66
3.1.5
CONCLUSION
.
69
3.2
A
BASIC
DESIGN
OF
A
PSEUDOMONAS
CHASSIS
FOR
REDOX-COFACTOR
DEPENDENT
BIOCATALYSIS
73
3.2.1
ABSTRACT
.
73
3.2.2
INTRODUCTION
.
73
3.2.3
DISCUSSION
.
89
3.2.4
CONCLUSION
.
91
3.3
HIGH
TITER
METHYL
KETONE
PRODUCTION
WITH
TAILORED
PSEUDOMONAS
TAIWANENSIS
V
LB120
95
3.3.1
ABSTRACT
.
95
3.3.2
INTRODUCTION
.
95
3.3.3
RESULTS
AND
DISCUSSION
.
97
3.3.4
CONCLUSION
.
110
3.4
METHYL
KETONE
PRODUCTION
IN
THE
PSEUDOMONAS
REDOX
CHASSIS.
115
3.4.1
ABSTRACT
.
115
3.4.2
INTRODUCTION
.
115
3.4.3
RESULTS
AND
DISCUSSION
.
116
3.4.4
CONCLUSION
.
121
4
GENERAL
DISCUSSION
AND
OUTLOOK
.
125
II
TABLE
OF
CONTENTS
4.1
HURDLES
AND
CHALLENGES
OF
CHASSIS
DEVELOPMENT
TO
PROVIDE
DESIRABLE
FUNCTIONS
AND
ENHANCED
PERFORMANCE
.
125
4.2
OPTIMIZATION
OF
METABOLIC
PATHWAY
RECONSTRUCTION
.
127
4.3
OPTIMIZATION
OF
BIOPROCESS
PARAMETERS
.
127
4.4
EXPANDING
THE
METHYL
KETONE
PORTFOLIO
.
129
4.5
CONCLUSION
.
130
5
APPENDIX.
133
6
REFERENCES.
137
7
CURRICULUM
VITAE
.
159
III |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Nies, Salome Clarissa 1987- |
| author_GND | (DE-588)1258749866 |
| author_facet | Nies, Salome Clarissa 1987- |
| author_role | aut |
| author_sort | Nies, Salome Clarissa 1987- |
| author_variant | s c n sc scn |
| building | Verbundindex |
| bvnumber | BV048253988 |
| collection | ebook |
| ctrlnum | (OCoLC)1322019310 (DE-599)DNB1257167227 |
| doi_str_mv | 10.18154/RWTH-2022-05073 |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048253988 |
| illustrated | Not Illustrated |
| index_date | 2024-07-03T19:57:45Z |
| indexdate | 2024-07-10T09:33:13Z |
| institution | BVB |
| institution_GND | (DE-588)1068101474 |
| isbn | 9783985550760 398555076X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033634264 |
| oclc_num | 1322019310 |
| open_access_boolean | 1 |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | XIII, 161 Seiten Diagramme 21 cm x 14.8 cm, 258 g |
| psigel | ebook |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| publisher | Apprimus Verlag |
| record_format | marc |
| series | Applied Microbiology |
| series2 | Applied Microbiology |
| spelling | Nies, Salome Clarissa 1987- Verfasser (DE-588)1258749866 aut Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies 1. Auflage Aachen Apprimus Verlag 2022 XIII, 161 Seiten Diagramme 21 cm x 14.8 cm, 258 g txt rdacontent n rdamedia nc rdacarrier Applied Microbiology 27 Dissertation RWTH Aachen University 2021 Methylgruppe (DE-588)4169721-2 gnd rswk-swf Herstellung (DE-588)4159653-5 gnd rswk-swf Stoffwechsel (DE-588)4057699-1 gnd rswk-swf Biokatalysator (DE-588)4006842-0 gnd rswk-swf Pseudomonas taiwanensis (DE-588)1064240615 gnd rswk-swf Fermentation (DE-588)4129537-7 gnd rswk-swf Pseudomonas (DE-588)4210233-9 gnd rswk-swf Fed-batch-Verfahren (DE-588)4372478-4 gnd rswk-swf Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Ketone (DE-588)4129416-6 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf microbes microbiology metabolic optimization (DE-588)4113937-9 Hochschulschrift gnd-content Biotechnologie (DE-588)4069491-4 s Pseudomonas (DE-588)4210233-9 s Stoffwechsel (DE-588)4057699-1 s DE-604 Pseudomonas taiwanensis (DE-588)1064240615 s Gentechnisch veränderter Organismus (DE-588)4353579-3 s Biokatalysator (DE-588)4006842-0 s Ketone (DE-588)4129416-6 s Methylgruppe (DE-588)4169721-2 s Herstellung (DE-588)4159653-5 s Fermentation (DE-588)4129537-7 s Fed-batch-Verfahren (DE-588)4372478-4 s Enzymkatalyse (DE-588)4152480-9 s Apprimus Verlag (DE-588)1068101474 pbl Erscheint auch als Online-Ausgabe, PDF https://doi.org/10.18154/RWTH-2022-05073 Applied Microbiology 27 (DE-604)BV042644516 27 https://doi.org/10.18154/RWTH-2022-05073 Resolving-System kostenfrei Volltext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033634264&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p vlb 20220510 DE-101 https://d-nb.info/provenance/plan#vlb |
| spellingShingle | Nies, Salome Clarissa 1987- Engineering Pseudomonas for the production of reduced compounds Applied Microbiology Methylgruppe (DE-588)4169721-2 gnd Herstellung (DE-588)4159653-5 gnd Stoffwechsel (DE-588)4057699-1 gnd Biokatalysator (DE-588)4006842-0 gnd Pseudomonas taiwanensis (DE-588)1064240615 gnd Fermentation (DE-588)4129537-7 gnd Pseudomonas (DE-588)4210233-9 gnd Fed-batch-Verfahren (DE-588)4372478-4 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Enzymkatalyse (DE-588)4152480-9 gnd Ketone (DE-588)4129416-6 gnd Biotechnologie (DE-588)4069491-4 gnd |
| subject_GND | (DE-588)4169721-2 (DE-588)4159653-5 (DE-588)4057699-1 (DE-588)4006842-0 (DE-588)1064240615 (DE-588)4129537-7 (DE-588)4210233-9 (DE-588)4372478-4 (DE-588)4353579-3 (DE-588)4152480-9 (DE-588)4129416-6 (DE-588)4069491-4 (DE-588)4113937-9 |
| title | Engineering Pseudomonas for the production of reduced compounds |
| title_auth | Engineering Pseudomonas for the production of reduced compounds |
| title_exact_search | Engineering Pseudomonas for the production of reduced compounds |
| title_exact_search_txtP | Engineering Pseudomonas for the production of reduced compounds |
| title_full | Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies |
| title_fullStr | Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies |
| title_full_unstemmed | Engineering Pseudomonas for the production of reduced compounds Salome Clarissa Nies |
| title_short | Engineering Pseudomonas for the production of reduced compounds |
| title_sort | engineering pseudomonas for the production of reduced compounds |
| topic | Methylgruppe (DE-588)4169721-2 gnd Herstellung (DE-588)4159653-5 gnd Stoffwechsel (DE-588)4057699-1 gnd Biokatalysator (DE-588)4006842-0 gnd Pseudomonas taiwanensis (DE-588)1064240615 gnd Fermentation (DE-588)4129537-7 gnd Pseudomonas (DE-588)4210233-9 gnd Fed-batch-Verfahren (DE-588)4372478-4 gnd Gentechnisch veränderter Organismus (DE-588)4353579-3 gnd Enzymkatalyse (DE-588)4152480-9 gnd Ketone (DE-588)4129416-6 gnd Biotechnologie (DE-588)4069491-4 gnd |
| topic_facet | Methylgruppe Herstellung Stoffwechsel Biokatalysator Pseudomonas taiwanensis Fermentation Pseudomonas Fed-batch-Verfahren Gentechnisch veränderter Organismus Enzymkatalyse Ketone Biotechnologie Hochschulschrift |
| url | https://doi.org/10.18154/RWTH-2022-05073 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033634264&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV042644516 |
| work_keys_str_mv | AT niessalomeclarissa engineeringpseudomonasfortheproductionofreducedcompounds AT apprimusverlag engineeringpseudomonasfortheproductionofreducedcompounds |