Rapid and efficient generation of microglia-like cells from human pluripotent stem cells: a versatile human in vitro platform to model the role of microglia in neurological diseases
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[2020]
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| Beschreibung: | XII, 124 Seiten Illustrationen, Diagramme 30 cm |
Internformat
MARC
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| 100 | 1 | |a Speicher, Anna Martina |d 1990- |e Verfasser |0 (DE-588)1248492145 |4 aut | |
| 245 | 1 | 0 | |a Rapid and efficient generation of microglia-like cells from human pluripotent stem cells |b a versatile human in vitro platform to model the role of microglia in neurological diseases |c vorgelegt von Anna Martina Speicher |
| 264 | 1 | |a Münster |c [2020] | |
| 300 | |a XII, 124 Seiten |b Illustrationen, Diagramme |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Westfälische Wilhelms-Universität Münster |d 2020 |g Beschränkt für den Austausch | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1248874064/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033578054&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033578054 | ||
| 883 | 0 | |8 1\p |a emakn |c 0,04936 |d 20220411 |q DE-101 |u https://d-nb.info/provenance/plan#emakn | |
| 883 | 2 | |8 2\p |a dnb |d 20220427 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
| 883 | 2 | |8 3\p |a dnb |d 20220427 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
Datensatz im Suchindex
| _version_ | 1804183959367581696 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.............................................................................................................................................
I
LIST
OF
ABBREVIATIONS
......................................................................................................................................
IV
LIST
OF
FIGURES
................................................................................................................................................
VII
LIST
OF
TABLES
.................................................................................................................................................
VIII
ABSTRACT
..........................................................................................................................................................
IX
ZUSAMMENFASSUNG
.......................................................................................................................................
XI
1.
INTRODUCTION
........................................................................................................................................
-1
1.1.
MICROGLIA
....................................................................................................................................
-1
1.1.1.
MICROGLIA
ONTOGENY
............................................................................................................
-2
1.1.2.
FUNCTIONS
OF
MICROGLIA
.......................................................................................................
-5
1.2.
HUMAN
PLURIPOTENT
STEM
CELLS
..................................................................................................
-
6
-
1.2.1.
HIPSC-DERIVED
MICROGLIA-LIKE
CELLS
...................................................................................
-
7
-
1.3.
FORWARD
PROGRAMMING
............................................................................................................
-10
1.3.1.
TET-ON
SYSTEM
................................................................................................................
-11
-
1.3.2.
GENOMIC
SAFE
HARBOURS
..................................................................................................
-12
1.3.3.
METHODS
OF
TARGETING
HPSCS
..........................................................................................
-13
-
1.4.
NEURODEGENERATION
...................................................................................................................
-14
-
1.4.1.
ALZHEIMER
S
DISEASE
........................................................................................................
-15
1.4.2.
PARKINSON
S
DISEASE
........................................................................................................
-15
-
1.4.3.
FRONTOTEMPORAL
LOBAR
DEGENERATION
...............................................................................
-16
-
1.4.4.
MICROGLIA
IN
NEURODEGENERATION
.....................................................................................
-16
-
1.5.
AIM
OF
THIS
PROJECT
.....................................................................................................................
-19
2.
MATERIALS
AND
METHODS
...................................................................................................................
-
20
-
2.1.
MATERIALS
..................................................................................................................................
-20
2.1.1.
CONSUMABLES
..................................................................................................................
-20
2.1.2.
EQUIPMENT
........................................................................................................................
-21
-
2.1.3.
KITS
...................................................................................................................................
-22
2.1.4.
PRIMARY
ANTIBODIES
..........................................................................................................
-22
2.1.5.
SECONDARY
ANTIBODIES
.....................................................................................................
-23
2.1.6.
ANTIBODIES
FOR
FLOW
CYTOMETRY
.........................................................................................
-23
-
2.1.7.
QPCR
PRIMERS
.................................................................................................................
-24
2.1.8.
CELL
LINES
..........................................................................................................................
-25
2.1.9.
PLASMIDS
..........................................................................................................................
-26
2.1.10.
CELL
CULTURE
CONSUMABLES
...........................................................................................
-
27
-
2.1.11.
CELL
CULTURE
MEDIA
.......................................................................................................
-28
2.2.
METHODS
...................................................................................................................................
-33
2.2.1.
MOLECULAR
BIOLOGY
.............................................................................................................
-33
2.2.1.1.
POLYMERASE
CHAIN
REACTION
...................................................................................
-
33
-
2.2.1.2.
AGAROSE
GEL
ELECTROPHORESIS
.............................................................................
....-34
2.2.1.3.
RESTRICTION
DIGEST
......................................................................................................
-
34
-
2.2.1.4.
GIBSON
ASSEMBLY
....................................................................................................
-34
2.2.1.5.
TRANSFORMATION
OF
CHEMICALLY
COMPETENT
E.
COLI
..................................................
-35
2.2.1.6.
ISOLATION
OF
PLASMID
DNA
.........................................................................................
-35
2.2.1.6.1.
MINI
PREPARATION
....................................................................................................
-35
2.2.1.6.2.
MIDI
PREPARATION
....................................................................................................
-
36
-
2.2.2.
CELL
CULTURE
.......................................................................................................................
-36
2.2.2.1.
MAINTENANCE
OF
HIPSCS
.............................................................................................
-
36
-
2.2.2.2.
FREEZING
AND
THAWING
OF
HIPSCS
AND
HNPCS
.........................................................
-
37
-
2.2.2.3.
NUCLEOFECTION
..............................................................................................................
-
37
-
2.2.2.3.1.
ROSA26
.................................................................................................................
-38
2.2.2.32.
AAVS1
...................................................................................................................
-38
2.2.2.33.
CLYBL
...................................................................................................................
-38
2.2.2.4.
DNA
ISOLATION
...............................................................................................................
-
38
-
2.2.2.5.
GENOTYPING
OF
GENETICALLY
MODIFIED
CELL
LINES
..........................................................
-
39
-
2.2.2.6.
INDUCTION
OF
GENETICALLY
MODIFIED
HIPSCS
FOR
MGL
GENERATION
...............................
39
-
2.2.2.7.
INDUCTION
OF
GENETICALLY
MODIFIED
HNPCS
FOR
HINEURON
GENERATION
.......................
-
40
-
2.2.2.8.
ISOLATION
OF
PERIPHERAL
BLOOD
MONONUCLEAR
CELLS
(PBMCS)
AND
SUBSEQUENT
DIFFERENTIATION
INTO
MACROPHAGES
...............................................................................................
-
40
-
2.2.3.
FLOW
CYTOMETRY
.................................................................................................................
-
41
-
2.2.4.
IMMUNOCYTOCHEMISTRY
.....................................................................................................
41
-
2.2.5.
RNA
ISOLATION
....................................................................................................................
-
42
-
2.2.6.
CDNA
SYNTHESIS
................................................................................................................-
42
-
2.2.7.
QUANTITATIVE
REAL-TIME
PCR
.............................................................................................
-
43
-
2.2.8.
PHAGOCYTOSIS
ASSAY
........................................................................................................
44
-
2.2.9.
ROS
MEASUREMENT
..........................................................................................................
-44
2.2.10.
CALCIUM
IMAGING
............................................................................................................
-
45
-
2.2.11.
OXYGEN
CONSUMPTION
(SEAHORSE
MEASUREMENT)
.......................................................
-
45
-
2.2.12.
MITOCHONDRIAL
NETWORK
ANALYSIS
..................................................................................
-
45
-
2.2.13.
APOPTOSIS
ASSAY
(FAM
FLICA
CASPASE
3/7)
.............................................................
-
46
-
2.2.14.
HUMAN
CORTICAL
BRAIN
ORGANOIDS
.................................................................................
-
46
-
2.2.15.
BULK
RNA
SEQUENCING
.................................................................................................
-47
3.
RESULTS
...............................................................................................................................................
-
50
-
3.1.
DEVELOPMENT
OF
A
FORWARD
PROGRAMMING
PROTOCOL
FOR
MGL
GENERATION
...............................
-
50
-
3.1.1.
CHOICE
OF
FORWARD
PROGRAMMING
FACTORS
FOR
HIPSC-DERIVED
MGLS
..............................
-
50
-
3.1.2.
PLASMID
GENERATION
..........................................................................................................
-
51
-
3.1.3.
GENERATION
AND
CHARACTERIZATION
OF
TARGETED
CELL
LINES
.........................
-
54 -
3.1.4.
COMPARISON
OF
INDUCTION
EFFICIENCY
IN
HIPSCS
EXPRESSING
DIFFERENT
TRANSCRIPTION
FACTOR
COMBINATIONS
..........................................................................................................................
-
56
-
3.1.5.
DEVELOPMENT
OF
SUITABLE
MEDIUM
COMPOSITIONS
............................................................
-
58 -
3.2.
PROTOCOL
FOR
MGL
GENERATION
...................................................................................................
-
60
-
3.2.1.
GENE
TARGETING
AND
TRANSGENE
EXPRESSION
IN
TWO
INDEPENDENT
HIPSC
LINES
..............
-
60
-
3.2.2.
CHARACTERIZATION
OF
HIPSC-DERIVED
MGLS
.....................................................................
-61
II
3.2.3.
MGL
GENE
EXPRESSION
PROFILE
........................................................................................
-63
-
3.2.4.
FUNCTIONALITY
OF
MGLS
.....................................................................................................
-64
3.2.4.1.
CYTOKINE
SECRETION
..................................................................................................
-
65
-
3.2.4.2.
MGL
PROLIFERATION
....................................................................................................
-
66
-
3.2.4.3.
PHAGOCYTOSIS
..........................................................................................................
-67
3.2.4.4.
ROS
PRODUCTION
AND
OXPHOS
POTENTIAL
.............................................................
-
68
-
3.2.4.5.
MGL
-
MACROPHAGE
COMPARISON
...................................................
-
69
-
3.2.4.6.
CALCIUM
LIVE
CELL
IMAGING
......................................................................................
-
70
-
3.2.4.7.
MGL
TRANSCRIPTOME
..................................................................................................
-71
3.2.5.
MGL
REPORTER
CELL
LINE
GENERATION
.................................................................................
-
72
-
3.2.5.1.
RTTA-IRES-MCHERRY
..................................................................................................
-73
3.2.52.
SHS231
.....................................................................................................................
-74
3.2.5.3.
CLYBL
.......................................................................................................................
-74
3.2.5.4.
COMPARISON
OF
DIFFERENT
REPORTER
CELL
LINES
.............................................................
-
76
-
3.3.
GENERATION
OF
INEURONS
FROM
HNPCS
USING
SINGLE
STEP
TARGETING
.......................................
-
77
-
3.3.1.
SINGLE
VECTOR
GENERATION
AND
TEST
.................................................................................
-
77
-
3.3.2.
PLASMID
GENERATION
AND
PROTOCOL
ESTABLISHMENT
..........................................................
-
79
-
3.3.3.
INEURON
REPORTER
CELL
LINE
...............................................................................................
-
82
-
3.4.
APPLICATIONS
............................................................................................................................
-82
3.4.1.
ESTABLISHMENT
OF
CO-CULTURE
SYSTEMS
............................................................................
-
82
-
3.4.2.
FTLD
DISEASE
MODELLING
USING
INEURON-MGL
CO-CULTURE
............................................
-
83
-
4.
DISCUSSION
.......................................................................................................................................
-86
4.1.
FORWARD
PROGRAMMING
AS
METHOD
FOR
MGL
GENERATION
.......................................................
87
-
4.2.
PROTOCOL
CONDITIONS
.................................................................................................................
88
-
4.3.
MICROGLIA
IDENTITY
.....................................................................................................................
-92
4.4.
REPORTER
CELL
LINE
GENERATION
..................................................................................................
93
-
4.5.
IN
VITRO
MODELLING
OF
NEURODEGENERATION
................................................................................
94
-
4.5.1.
ROLE
OF
MICROGLIA
IN
FTLD
.............................................................................................
-
95
-
4.5.2.
IPSC-DERIVED
CELLS
FOR
THE
INVESTIGATION
OF
AGE-RELATED
DISEASES
..............................
-
98
-
4.1.
INEURON
FORWARD
PROGRAMMING
PROTOCOL
................................................................................
-99
-
4.2.
CONCLUDING
REMARKS
...........................................................................................................
-100
-
5.
REFERENCES
...................................................................................................................................
-102
6.
APPENDIX
.......................................................................................................................................
-120
6.1.
ADDITIONAL
PLASMIDS
.............................................................................................................
-120
6.2.
DIFFERENTIATION
OF
HIPSCS
INTO
CELLS
OF
ALL
THREE
GERM
LAYERS
..............................................
-121
-
6.3.
PROTEOME
PROFILER
.................................................................................................................
-121
-
6.4.
REPORTER
CELL
LINES
..............................................................................................................
-122
-
6.5.
SINGLE
VECTOR
APPROACH
.......................................................................................................
-122
-
III
|
| adam_txt |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.
I
LIST
OF
ABBREVIATIONS
.
IV
LIST
OF
FIGURES
.
VII
LIST
OF
TABLES
.
VIII
ABSTRACT
.
IX
ZUSAMMENFASSUNG
.
XI
1.
INTRODUCTION
.
-1
1.1.
MICROGLIA
.
-1
1.1.1.
MICROGLIA
ONTOGENY
.
-2
1.1.2.
FUNCTIONS
OF
MICROGLIA
.
-5
1.2.
HUMAN
PLURIPOTENT
STEM
CELLS
.
-
6
-
1.2.1.
HIPSC-DERIVED
MICROGLIA-LIKE
CELLS
.
-
7
-
1.3.
FORWARD
PROGRAMMING
.
-10
1.3.1.
TET-ON
SYSTEM
.
-11
-
1.3.2.
GENOMIC
SAFE
HARBOURS
.
-12
1.3.3.
METHODS
OF
TARGETING
HPSCS
.
-13
-
1.4.
NEURODEGENERATION
.
-14
-
1.4.1.
ALZHEIMER
'
S
DISEASE
.
-15
1.4.2.
PARKINSON
'
S
DISEASE
.
-15
-
1.4.3.
FRONTOTEMPORAL
LOBAR
DEGENERATION
.
-16
-
1.4.4.
MICROGLIA
IN
NEURODEGENERATION
.
-16
-
1.5.
AIM
OF
THIS
PROJECT
.
-19
2.
MATERIALS
AND
METHODS
.
-
20
-
2.1.
MATERIALS
.
-20
2.1.1.
CONSUMABLES
.
-20
2.1.2.
EQUIPMENT
.
-21
-
2.1.3.
KITS
.
-22
2.1.4.
PRIMARY
ANTIBODIES
.
-22
2.1.5.
SECONDARY
ANTIBODIES
.
-23
2.1.6.
ANTIBODIES
FOR
FLOW
CYTOMETRY
.
-23
-
2.1.7.
QPCR
PRIMERS
.
-24
2.1.8.
CELL
LINES
.
-25
2.1.9.
PLASMIDS
.
-26
2.1.10.
CELL
CULTURE
CONSUMABLES
.
-
27
-
2.1.11.
CELL
CULTURE
MEDIA
.
-28
2.2.
METHODS
.
-33
2.2.1.
MOLECULAR
BIOLOGY
.
-33
2.2.1.1.
POLYMERASE
CHAIN
REACTION
.
-
33
-
2.2.1.2.
AGAROSE
GEL
ELECTROPHORESIS
.
.-34
2.2.1.3.
RESTRICTION
DIGEST
.
-
34
-
2.2.1.4.
GIBSON
ASSEMBLY
.
-34
2.2.1.5.
TRANSFORMATION
OF
CHEMICALLY
COMPETENT
E.
COLI
.
-35
2.2.1.6.
ISOLATION
OF
PLASMID
DNA
.
-35
2.2.1.6.1.
MINI
PREPARATION
.
-35
2.2.1.6.2.
MIDI
PREPARATION
.
-
36
-
2.2.2.
CELL
CULTURE
.
-36
2.2.2.1.
MAINTENANCE
OF
HIPSCS
.
-
36
-
2.2.2.2.
FREEZING
AND
THAWING
OF
HIPSCS
AND
HNPCS
.
-
37
-
2.2.2.3.
NUCLEOFECTION
.
-
37
-
2.2.2.3.1.
ROSA26
.
-38
2.2.2.32.
AAVS1
.
-38
2.2.2.33.
CLYBL
.
-38
2.2.2.4.
DNA
ISOLATION
.
-
38
-
2.2.2.5.
GENOTYPING
OF
GENETICALLY
MODIFIED
CELL
LINES
.
-
39
-
2.2.2.6.
INDUCTION
OF
GENETICALLY
MODIFIED
HIPSCS
FOR
MGL
GENERATION
.
39
-
2.2.2.7.
INDUCTION
OF
GENETICALLY
MODIFIED
HNPCS
FOR
HINEURON
GENERATION
.
-
40
-
2.2.2.8.
ISOLATION
OF
PERIPHERAL
BLOOD
MONONUCLEAR
CELLS
(PBMCS)
AND
SUBSEQUENT
DIFFERENTIATION
INTO
MACROPHAGES
.
-
40
-
2.2.3.
FLOW
CYTOMETRY
.
-
41
-
2.2.4.
IMMUNOCYTOCHEMISTRY
.
41
-
2.2.5.
RNA
ISOLATION
.
-
42
-
2.2.6.
CDNA
SYNTHESIS
.-
42
-
2.2.7.
QUANTITATIVE
REAL-TIME
PCR
.
-
43
-
2.2.8.
PHAGOCYTOSIS
ASSAY
.
44
-
2.2.9.
ROS
MEASUREMENT
.
-44
2.2.10.
CALCIUM
IMAGING
.
-
45
-
2.2.11.
OXYGEN
CONSUMPTION
(SEAHORSE
MEASUREMENT)
.
-
45
-
2.2.12.
MITOCHONDRIAL
NETWORK
ANALYSIS
.
-
45
-
2.2.13.
APOPTOSIS
ASSAY
(FAM
FLICA
CASPASE
3/7)
.
-
46
-
2.2.14.
HUMAN
CORTICAL
BRAIN
ORGANOIDS
.
-
46
-
2.2.15.
BULK
RNA
SEQUENCING
.
-47
3.
RESULTS
.
-
50
-
3.1.
DEVELOPMENT
OF
A
FORWARD
PROGRAMMING
PROTOCOL
FOR
MGL
GENERATION
.
-
50
-
3.1.1.
CHOICE
OF
FORWARD
PROGRAMMING
FACTORS
FOR
HIPSC-DERIVED
MGLS
.
-
50
-
3.1.2.
PLASMID
GENERATION
.
-
51
-
3.1.3.
GENERATION
AND
CHARACTERIZATION
OF
TARGETED
CELL
LINES
.
-
54 -
3.1.4.
COMPARISON
OF
INDUCTION
EFFICIENCY
IN
HIPSCS
EXPRESSING
DIFFERENT
TRANSCRIPTION
FACTOR
COMBINATIONS
.
-
56
-
3.1.5.
DEVELOPMENT
OF
SUITABLE
MEDIUM
COMPOSITIONS
.
-
58 -
3.2.
PROTOCOL
FOR
MGL
GENERATION
.
-
60
-
3.2.1.
GENE
TARGETING
AND
TRANSGENE
EXPRESSION
IN
TWO
INDEPENDENT
HIPSC
LINES
.
-
60
-
3.2.2.
CHARACTERIZATION
OF
HIPSC-DERIVED
MGLS
.
-61
II
3.2.3.
MGL
GENE
EXPRESSION
PROFILE
.
-63
-
3.2.4.
FUNCTIONALITY
OF
MGLS
.
-64
3.2.4.1.
CYTOKINE
SECRETION
.
-
65
-
3.2.4.2.
MGL
PROLIFERATION
.
-
66
-
3.2.4.3.
PHAGOCYTOSIS
.
-67
3.2.4.4.
ROS
PRODUCTION
AND
OXPHOS
POTENTIAL
.
-
68
-
3.2.4.5.
MGL
-
MACROPHAGE
COMPARISON
.
-
69
-
3.2.4.6.
CALCIUM
LIVE
CELL
IMAGING
.
-
70
-
3.2.4.7.
MGL
TRANSCRIPTOME
.
-71
3.2.5.
MGL
REPORTER
CELL
LINE
GENERATION
.
-
72
-
3.2.5.1.
RTTA-IRES-MCHERRY
.
-73
3.2.52.
SHS231
.
-74
3.2.5.3.
CLYBL
.
-74
3.2.5.4.
COMPARISON
OF
DIFFERENT
REPORTER
CELL
LINES
.
-
76
-
3.3.
GENERATION
OF
INEURONS
FROM
HNPCS
USING
SINGLE
STEP
TARGETING
.
-
77
-
3.3.1.
SINGLE
VECTOR
GENERATION
AND
TEST
.
-
77
-
3.3.2.
PLASMID
GENERATION
AND
PROTOCOL
ESTABLISHMENT
.
-
79
-
3.3.3.
INEURON
REPORTER
CELL
LINE
.
-
82
-
3.4.
APPLICATIONS
.
-82
3.4.1.
ESTABLISHMENT
OF
CO-CULTURE
SYSTEMS
.
-
82
-
3.4.2.
FTLD
DISEASE
MODELLING
USING
INEURON-MGL
CO-CULTURE
.
-
83
-
4.
DISCUSSION
.
-86
4.1.
FORWARD
PROGRAMMING
AS
METHOD
FOR
MGL
GENERATION
.
87
-
4.2.
PROTOCOL
CONDITIONS
.
88
-
4.3.
MICROGLIA
IDENTITY
.
-92
4.4.
REPORTER
CELL
LINE
GENERATION
.
93
-
4.5.
IN
VITRO
MODELLING
OF
NEURODEGENERATION
.
94
-
4.5.1.
ROLE
OF
MICROGLIA
IN
FTLD
.
-
95
-
4.5.2.
IPSC-DERIVED
CELLS
FOR
THE
INVESTIGATION
OF
AGE-RELATED
DISEASES
.
-
98
-
4.1.
INEURON
FORWARD
PROGRAMMING
PROTOCOL
.
-99
-
4.2.
CONCLUDING
REMARKS
.
-100
-
5.
REFERENCES
.
-102
6.
APPENDIX
.
-120
6.1.
ADDITIONAL
PLASMIDS
.
-120
6.2.
DIFFERENTIATION
OF
HIPSCS
INTO
CELLS
OF
ALL
THREE
GERM
LAYERS
.
-121
-
6.3.
PROTEOME
PROFILER
.
-121
-
6.4.
REPORTER
CELL
LINES
.
-122
-
6.5.
SINGLE
VECTOR
APPROACH
.
-122
-
III |
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| any_adam_object_boolean | 1 |
| author | Speicher, Anna Martina 1990- |
| author_GND | (DE-588)1248492145 |
| author_facet | Speicher, Anna Martina 1990- |
| author_role | aut |
| author_sort | Speicher, Anna Martina 1990- |
| author_variant | a m s am ams |
| building | Verbundindex |
| bvnumber | BV048196955 |
| ctrlnum | (OCoLC)1293293439 (DE-599)DNB1248874064 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048196955 |
| illustrated | Illustrated |
| index_date | 2024-07-03T19:45:29Z |
| indexdate | 2024-07-10T09:31:44Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033578054 |
| oclc_num | 1293293439 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | XII, 124 Seiten Illustrationen, Diagramme 30 cm |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| record_format | marc |
| spelling | Speicher, Anna Martina 1990- Verfasser (DE-588)1248492145 aut Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases vorgelegt von Anna Martina Speicher Münster [2020] XII, 124 Seiten Illustrationen, Diagramme 30 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Westfälische Wilhelms-Universität Münster 2020 Beschränkt für den Austausch (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1248874064/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033578054&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p emakn 0,04936 20220411 DE-101 https://d-nb.info/provenance/plan#emakn 2\p dnb 20220427 DE-101 https://d-nb.info/provenance/plan#dnb 3\p dnb 20220427 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Speicher, Anna Martina 1990- Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases |
| subject_GND | (DE-588)4113937-9 |
| title | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases |
| title_auth | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases |
| title_exact_search | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases |
| title_exact_search_txtP | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases |
| title_full | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases vorgelegt von Anna Martina Speicher |
| title_fullStr | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases vorgelegt von Anna Martina Speicher |
| title_full_unstemmed | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases vorgelegt von Anna Martina Speicher |
| title_short | Rapid and efficient generation of microglia-like cells from human pluripotent stem cells |
| title_sort | rapid and efficient generation of microglia like cells from human pluripotent stem cells a versatile human in vitro platform to model the role of microglia in neurological diseases |
| title_sub | a versatile human in vitro platform to model the role of microglia in neurological diseases |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1248874064/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033578054&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT speicherannamartina rapidandefficientgenerationofmicroglialikecellsfromhumanpluripotentstemcellsaversatilehumaninvitroplatformtomodeltheroleofmicrogliainneurologicaldiseases |
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