Homeostatic mitophagy scales mitochondrial networks:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Köln
[2021]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | Literaturverzeichnis: Seite 129-153 |
| Beschreibung: | 156 Seiten Illustrationen, Diagramme 21 cm |
Internformat
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Datensatz im Suchindex
| _version_ | 1804183953490313216 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.................................................................................................................................
1
FIGURE
LEGENDS
.....................................................................................................................................
3
TABLE
LEGENDS
.......................................................................................................................................
4
ACKNOWLEDGEMENTS
..............................................................................................................................
5
ABBREVIATIONS
........................................................................................................................................
7
ABSTRACT
...............................................................................................................................................
11
ZUSAMMENFASSUNG
.............................................................................................................................
12
INTRODUCTION
.........................................................................................................................................
13
HOMEOSTATIC
MECHANISMS
WITHIN
THE
EUKARYOTIC
ORGANISM
...............................................................
13
PROTEOSTASIS
......................................................................................................................................
13
AUTOPHAGY
........................................................................................................................................
15
AUTOPHAGY
IN
YEAST
.......................................................................................................................
17
METABOLISM
.......................................................................................................................................
22
ENERGY
SENSING
&
HOMEOSTASIS
....................................................................................................
22
METHODS
OF
ATP
GENERATION
........................................................................................................
23
TCA
&
OXIDATIVE
PHOSPHORYLATION................................................................................................
26
OXIDATIVE
PHOSPHORYLATION
............................................................................................................
27
GLUCOSE
EFFECTS
ON
METABOLISM
....................................................................................................
29
MITOCHONDRIA
.......................................................................................................................................
30
ORIGIN
............................................................................................................................................
31
FUNCTION
.......................................................................................................................................
31
STRUCTURE
.......................................................................................................................................
32
MITOCHONDRIAL
LIPIDS
.....................................................................................................................
32
THE
MTDNA
..................................................................................................................................
37
MITOPHAGY
.....................................................................................................................................
38
ORGANELLE
SCALING
AND
HOMEOSTASIS
..................................................................................................
40
AIMS
.....................................................................................................................................................
44
RESULTS
.................................................................................................................................................
46
ASSESSING
THE
EFFECTS
OF
BLOCKED
MITOPHAGY
ON MITOCHONDRIAL
NETWORKS
DURING
STARVATION
.............
46
THE
CONSEQUENCE
OF
NITROGEN
STARVATION
AND
MITOPHAGY
ON
THE
VOLUME
OF
THE
CELL,
VACUOLE
AND
OTHER
ORGANELLES
................................................................................................................................
53
FUNCTIONAL
ASSESSMENT
OF
THE
MITOCHONDRIA
.....................................................................................
57
COMPOSITIONAL
ASSESSMENT
OF
MITOCHONDRIA
IN
DEPENDENCE
OF
AUTOPHAGY
......................................
62
ASSESSMENT
OF
ANY
METABOLIC
CHANGE
BETWEEN
WT
AND
AMITOPHAGY
CELLS
.....................................
73
DISSECTING
THE
DYNAMICS
OF
THE
INCREASE
IN
MITOCHONDRIAL
VOLUME
IN
AMITOPHAGY
CELLS.....................
76
DISCUSSION
...........................................................................................................................................
95
THE
ROLE
OF
NON-SELECTIVE
AND
SELECTIVE
AUTOPHAGY
IN
THE
EXPANSION
OF
THE
MITOCHONDRIA...............
97
1
IS
THE
VOLUME
OF
THE
MITOCHONDRIA
SENSED?
.......................................................................................
99
WHY
FLUX
MATERIAL
INTO
ORGANELLES DURING
STARVATION?
......................................................................
100
DOES THE
ORIGIN
OF
MITOCHONDRIA
OFFER
INSIGHTS
INTO
THE ROLE
OF
MITOPHAGY
IN
SIZE
REGULATION?
.......
101
DOES
ATG32
INFLUENCE
OTHER
ASPECTS
OF
THE
CELL
OUTSIDE
OF
MITOPHAGY?
..........................................
105
FUTURE
OUTLOOKS
AND
IDEAS
...............................................................................................................
107
CONCLUSION
........................................................................................................................................
109
MATERIALS
AND
METHODS
......................................................................................................................
110
STRAINS
AND
PLASMIDS
........................................................................................................................
110
YEAST
MEDIA
AND
GROWTH
..................................................................................................................
111
BACTERIA
MEDIA
AND
GROWTH
CONDITIONS
.......................................................................................
112
RITE-SYSTEM
...............................................................................................................................
112
OMIC
APPROACHES
...........................................................................................................................
113
LIPIDOMIC
&
METABOLIC
ANALYSIS
...................................................................................................
113
PROTEOMICS
(SILAC
AND
NON-SILAC)
..........................................................................................
113
PROTEOMIC
EXTRACTION
...................................................................................................................
113
PROTEOMICS
DATA
ANALYSIS
............................................................................................................
115
SILAC
DATA
ANALYSIS
....................................................................................................................
116
LIPIDOMIC
AND
METABOLIC
EXTRACTION
.............................................................................................
117
LIQUID
CHROMATOGRAPHY-HIGH
RESOLUTION
MASS
SPECTROMETRY-BASED
(LC-HRMS)
ANALYSIS
OF
LIPIDS
.............................................................................................................................................
117
ANION-EXCHANGE
CHROMATOGRAPHY
MASS
SPECTROMETRY
(AEX-MS)
FOR
THE
ANALYSIS
OF
ANIONIC
METABOLITES
..................................................................................................................................
118
UNTARGETED
LIQUID
CHROMATOGRAPHY-HIGH-RESOLUTION
MASS
SPECTROMETRY-BASED
(LC-HRS-MS)
ANALYSIS
OF
AMINE-CONTAINING
METABOLITES....................................................................................
119
IMAGING
AND
FLOW
CYTOMETRY
...........................................................................................................
120
FLUORESCENCE
MICROSCOPY
...........................................................................................................
120
FLUORESCENCE
MICROSCOPY
FOR
THE
RITE,
ERMES,
MTDNA,
AND
LIPID
DROPLETS
...........................
121
ELECTRON
MICROSCOPY
....................................................................................................................
121
MITOGRAPH
ANALYSIS
.....................................................................................................................
121
STAINING
.......................................................................................................................................
122
VACUOLE
-
FM4-64
........................................................................................................................
122
LIPID
DROPLETS
...............................................................................................................................
122
DAPI
-
MITOCHONDRIAL
DNA
ERROR!
BOOKMARK
NOT
DEFINED.
MOLECULAR
AND
CELLULAR
BIOLOGY
........................................................................................................
123
WHOLE
CELL
EXTRACTION,
WESTERN
BLOT
ANALYSIS,
AND
QUANTIFICATION
................................................
123
QPCR
...........................................................................................................................................
123
SEAHORSE
.....................................................................................................................................
124
MOLECULAR
BIOLOGY
........................................................................................................................
124
BIOINFORMATICS
..............................................................................................................................
125
BIBLIOGRAPHY
.......................................................................................................................................
129
ERKLARUNG
............................................................................................................................................
154
2
|
| adam_txt |
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS
.
1
FIGURE
LEGENDS
.
3
TABLE
LEGENDS
.
4
ACKNOWLEDGEMENTS
.
5
ABBREVIATIONS
.
7
ABSTRACT
.
11
ZUSAMMENFASSUNG
.
12
INTRODUCTION
.
13
HOMEOSTATIC
MECHANISMS
WITHIN
THE
EUKARYOTIC
ORGANISM
.
13
PROTEOSTASIS
.
13
AUTOPHAGY
.
15
AUTOPHAGY
IN
YEAST
.
17
METABOLISM
.
22
ENERGY
SENSING
&
HOMEOSTASIS
.
22
METHODS
OF
ATP
GENERATION
.
23
TCA
&
OXIDATIVE
PHOSPHORYLATION.
26
OXIDATIVE
PHOSPHORYLATION
.
27
GLUCOSE
EFFECTS
ON
METABOLISM
.
29
MITOCHONDRIA
.
30
ORIGIN
.
31
FUNCTION
.
31
STRUCTURE
.
32
MITOCHONDRIAL
LIPIDS
.
32
THE
MTDNA
.
37
MITOPHAGY
.
38
ORGANELLE
SCALING
AND
HOMEOSTASIS
.
40
AIMS
.
44
RESULTS
.
46
ASSESSING
THE
EFFECTS
OF
BLOCKED
MITOPHAGY
ON MITOCHONDRIAL
NETWORKS
DURING
STARVATION
.
46
THE
CONSEQUENCE
OF
NITROGEN
STARVATION
AND
MITOPHAGY
ON
THE
VOLUME
OF
THE
CELL,
VACUOLE
AND
OTHER
ORGANELLES
.
53
FUNCTIONAL
ASSESSMENT
OF
THE
MITOCHONDRIA
.
57
COMPOSITIONAL
ASSESSMENT
OF
MITOCHONDRIA
IN
DEPENDENCE
OF
AUTOPHAGY
.
62
ASSESSMENT
OF
ANY
METABOLIC
CHANGE
BETWEEN
WT
AND
AMITOPHAGY
CELLS
.
73
DISSECTING
THE
DYNAMICS
OF
THE
INCREASE
IN
MITOCHONDRIAL
VOLUME
IN
AMITOPHAGY
CELLS.
76
DISCUSSION
.
95
THE
ROLE
OF
NON-SELECTIVE
AND
SELECTIVE
AUTOPHAGY
IN
THE
EXPANSION
OF
THE
MITOCHONDRIA.
97
1
IS
THE
VOLUME
OF
THE
MITOCHONDRIA
SENSED?
.
99
WHY
FLUX
MATERIAL
INTO
ORGANELLES DURING
STARVATION?
.
100
DOES THE
ORIGIN
OF
MITOCHONDRIA
OFFER
INSIGHTS
INTO
THE ROLE
OF
MITOPHAGY
IN
SIZE
REGULATION?
.
101
DOES
ATG32
INFLUENCE
OTHER
ASPECTS
OF
THE
CELL
OUTSIDE
OF
MITOPHAGY?
.
105
FUTURE
OUTLOOKS
AND
IDEAS
.
107
CONCLUSION
.
109
MATERIALS
AND
METHODS
.
110
STRAINS
AND
PLASMIDS
.
110
YEAST
MEDIA
AND
GROWTH
.
111
BACTERIA
MEDIA
AND
GROWTH
CONDITIONS
.
112
RITE-SYSTEM
.
112
OMIC
APPROACHES
.
113
LIPIDOMIC
&
METABOLIC
ANALYSIS
.
113
PROTEOMICS
(SILAC
AND
NON-SILAC)
.
113
PROTEOMIC
EXTRACTION
.
113
PROTEOMICS
DATA
ANALYSIS
.
115
SILAC
DATA
ANALYSIS
.
116
LIPIDOMIC
AND
METABOLIC
EXTRACTION
.
117
LIQUID
CHROMATOGRAPHY-HIGH
RESOLUTION
MASS
SPECTROMETRY-BASED
(LC-HRMS)
ANALYSIS
OF
LIPIDS
.
117
ANION-EXCHANGE
CHROMATOGRAPHY
MASS
SPECTROMETRY
(AEX-MS)
FOR
THE
ANALYSIS
OF
ANIONIC
METABOLITES
.
118
UNTARGETED
LIQUID
CHROMATOGRAPHY-HIGH-RESOLUTION
MASS
SPECTROMETRY-BASED
(LC-HRS-MS)
ANALYSIS
OF
AMINE-CONTAINING
METABOLITES.
119
IMAGING
AND
FLOW
CYTOMETRY
.
120
FLUORESCENCE
MICROSCOPY
.
120
FLUORESCENCE
MICROSCOPY
FOR
THE
RITE,
ERMES,
MTDNA,
AND
LIPID
DROPLETS
.
121
ELECTRON
MICROSCOPY
.
121
MITOGRAPH
ANALYSIS
.
121
STAINING
.
122
VACUOLE
-
FM4-64
.
122
LIPID
DROPLETS
.
122
DAPI
-
MITOCHONDRIAL
DNA
ERROR!
BOOKMARK
NOT
DEFINED.
MOLECULAR
AND
CELLULAR
BIOLOGY
.
123
WHOLE
CELL
EXTRACTION,
WESTERN
BLOT
ANALYSIS,
AND
QUANTIFICATION
.
123
QPCR
.
123
SEAHORSE
.
124
MOLECULAR
BIOLOGY
.
124
BIOINFORMATICS
.
125
BIBLIOGRAPHY
.
129
ERKLARUNG
.
154
2 |
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| index_date | 2024-07-03T19:45:03Z |
| indexdate | 2024-07-10T09:31:38Z |
| institution | BVB |
| institution_GND | (DE-588)2024231-1 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033575658 |
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| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-83 |
| owner_facet | DE-355 DE-BY-UBR DE-83 |
| physical | 156 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2021 |
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| record_format | marc |
| spelling | Thomas, Ryan Lee Verfasser (DE-588)1239761732 aut Homeostatic mitophagy scales mitochondrial networks vorgelegt von Ryan Lee Thomas Köln [2021] 156 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Literaturverzeichnis: Seite 129-153 Dissertation Universität zu Köln 2021 (DE-588)4113937-9 Hochschulschrift gnd-content Universität zu Köln (DE-588)2024231-1 dgg B:DE-101 application/pdf https://d-nb.info/1248176685/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033575658&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p aepsg 0,95389 20220428 DE-101 https://d-nb.info/provenance/plan#aepsg |
| spellingShingle | Thomas, Ryan Lee Homeostatic mitophagy scales mitochondrial networks |
| subject_GND | (DE-588)4113937-9 |
| title | Homeostatic mitophagy scales mitochondrial networks |
| title_auth | Homeostatic mitophagy scales mitochondrial networks |
| title_exact_search | Homeostatic mitophagy scales mitochondrial networks |
| title_exact_search_txtP | Homeostatic mitophagy scales mitochondrial networks |
| title_full | Homeostatic mitophagy scales mitochondrial networks vorgelegt von Ryan Lee Thomas |
| title_fullStr | Homeostatic mitophagy scales mitochondrial networks vorgelegt von Ryan Lee Thomas |
| title_full_unstemmed | Homeostatic mitophagy scales mitochondrial networks vorgelegt von Ryan Lee Thomas |
| title_short | Homeostatic mitophagy scales mitochondrial networks |
| title_sort | homeostatic mitophagy scales mitochondrial networks |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1248176685/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033575658&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT thomasryanlee homeostaticmitophagyscalesmitochondrialnetworks AT universitatzukoln homeostaticmitophagyscalesmitochondrialnetworks |
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