PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS):
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Aachen
[2021]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 87 Seiten Illustrationen, Diagramme 21 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |c vorgelegt von Ting Jia |
| 264 | 1 | |a Aachen |c [2021] | |
| 300 | |a 87 Seiten |b Illustrationen, Diagramme |c 21 cm | ||
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Datensatz im Suchindex
| _version_ | 1804183949026525184 |
|---|---|
| adam_text | CONTENT
1.
INTRODUCTION
.............................................................................................
1
1.1
FOCAL
SEGMENTAL
GLOMERULOSCLEROSIS
(FSGS)
..........................................
1
1.2
MECHANISMS
OF
FSGS
.............................................................................
2
1.2.1
PODOCYTES
IN
FSGS
.......................................................................
2
1.2.2
PECS
IN
FSGS
................................................................................
3
1.3
FSGS
ANIMAL
MODELS
...............................................................................
5
1.3.1
SURGICAL
&
NEPHROTOXIC
ANIMAL
MODELS
...........................................
5
1.3.2
TRANSGENIC
ANIMAL
MODELS
.............................................................
6
1.4
PDGFS
AND
THEIR
RECEPTORS
IN
RENAL
DEVELOPMENT
AND
DISEASE
...............
8
1.4.1
PDGFS
AND
PDGF
RECEPTORS
.........................................................
8
1.4.2
PDGFS
AND
PDGF
RECEPTORS
IN
RENAL
DEVELOPMENT
.......................
9
1.4.3
PDGFS
IN
GLOMERULAR
CELLS
IN
VITRO
................................................
10
1.4.4
PDGFR-P
AND
ITS
LIGANDS
IN
RENAL
DISEASE
...................................
10
2.
MOTIVATION
AND
AIMS
..............................................................................
12
3.
MATERIALSAND
METHODS
......................................................................
13
3.1
DEVICES
..................................................................................................
13
3.2
CONSUMABLES
.........................................................................................
14
3.3
REAGENTS
...............................................................................................
15
3.4
IN
VIVO
EXPERIMENTS
................................................................................
17
3.4.1
THIRTY-FIVE
WEEKS
OLD
THY
1.1
TRANSGENIC
MICE
..............................
17
3.4.2
MODELS
WITH
FSGS
........................................................................
17
3.4.3
NON-INVASIVE
BLOOD
PRESSURE
........................................................
18
3.4.4
KIDNEY
HARVEST
AND
FIXATION
..........................................................
18
3.5
HUMAN
SAMPLES
.....................................................................................
19
3.6
HISTOLOGY
AND
MORPHOLOGY
.....................................................................
19
3.6.1
PERIODIC
ACID
SCHIFF
(PAS)
............................................................
20
3.6.2
IMMUNOHISTOCHEMISTRY
(IHC)
.......................................................
21
3.6.3
IMMUNOFLUORESCENCE
(IF)
.............................................................
24
3.6.4
FLUORESCENCE
IN
SITU
HYBRIDIZATION
(FISH)
....................................
24
3.7
IN
VITRO
CELL
CULTURE
EXPERIMENTS
.............................................................
26
3.7.1
THAWING
AND
CULTURE
OF
CELLS
.........................................................
26
3.7.2
CULTURE
&
SEEDING
OF
PECS
...........................................................
26
3.7.3
PECS
STIMULATION
..........................................................................
27
3.7.4
PROLIFERATION
ASSAY
........................................................................
27
3.7.5 WOUND
HEALING
AND
MIGRATION
ASSAYS
..........................................
29
3.8
PROTEIN
EXTRACTION
AND
ANALYSIS
..............................................................
30
3.8.1
PROTEIN
EXTRACTION
FROM
CELLS
.........................................................
30
3.8.2
SDS-PAGE
....................................................................................
30
3.8.3
WESTERN
BLOT
..................................................................................
31
3.9
MRNA
EXTRACTION
AND
ANALYSIS
...............................................................
32
3.9.1
RNA
EXTRACTION
FROM
CELLS
.............................................................
32
3.9.2
CDNA-SYNTHESIS
............................................................................
33
3.9.3
REAL-TIME
QUANTITATIVE
PCR
(RT-QPCR)
........................................
34
3.10
STATISTICAL
ANALYSIS
................................................................................
36
4.
RESULTS
........................................................................................................
37
4.1
SPONTANEOUS
PHENOTYPE
OF
35
WEEKS
OLD
THY1
.1
TRANSGENIC
MICE
......
37
4.2
CHARACTERIZATION
OF
THE
PROGRESSIVE
FSGS
THY1.1
MOUSE
MODEL
........
40
4.3 PDGFR-P
AND
ITS
LIGANDS
PDGF-B
AND
PDGF-D
WERE
UPREGULATED
IN
THE
FSGSTHY1.1
MOUSE
MODEL
.........................................................................
50
4.4
KIDNEY
TUBULOINTERSTITIAL
FIBROSIS
WAS
INDUCED
IN
THY1
.1
FSGS
MODELS
.
55
4.5
PDGFR-P
AND
ITS
LIGANDS
PDGF-B
AND
PDGF-D
WERE
UPREGULATED
IN
HUMAN
FSGS
................................................................................................
57
4.6
STIMULATION
OF
PRIMARY
MOUSE
PECS
......................................................
59
4.6.1
TGF-PI
STIMULATION
IN
PRIMARY
PECS
............................................
59
4.6.2
PDGF-B
STIMULATION
IN
PRIMARY
PECS
..........................................
62
4.6.3
PDGF-D
STIMULATION
IN
PRIMARY
PECS
..........................................
64
5.
DISCUSSION
..................................................................................................
68
5.1
SPONTANEOUS
PHENOTYPE
OF
35
WEEKS
OLD
THY1.1
TRANSGENIC
MICE
...
68
5.2
CHARACTERIZATION
AND
MECHANISM
OF
THE
FSGS
THY1.1
MOUSE
MODEL
.69
5.3
PDGFR-P
AND
ITS
LIGANDS
PDGF-B
AND
PDGF-D
IN
FSGS
................
71
6.
SUMMARY
.....................................................................................................
74
7.
ZUSAMMENFASSUNG
.................................................................................
75
8.
REFERENCES
..............................................................................................
76
9.
PUBLICATION
...............................................................................................
83
10.
ACKNOWLEDGEMENT
...............................................................................
84
11.
AFFIDAVITS
.................................................................................................
85
12.
CURRICULUM
VITAE
..................................................................................
87
|
| adam_txt |
CONTENT
1.
INTRODUCTION
.
1
1.1
FOCAL
SEGMENTAL
GLOMERULOSCLEROSIS
(FSGS)
.
1
1.2
MECHANISMS
OF
FSGS
.
2
1.2.1
PODOCYTES
IN
FSGS
.
2
1.2.2
PECS
IN
FSGS
.
3
1.3
FSGS
ANIMAL
MODELS
.
5
1.3.1
SURGICAL
&
NEPHROTOXIC
ANIMAL
MODELS
.
5
1.3.2
TRANSGENIC
ANIMAL
MODELS
.
6
1.4
PDGFS
AND
THEIR
RECEPTORS
IN
RENAL
DEVELOPMENT
AND
DISEASE
.
8
1.4.1
PDGFS
AND
PDGF
RECEPTORS
.
8
1.4.2
PDGFS
AND
PDGF
RECEPTORS
IN
RENAL
DEVELOPMENT
.
9
1.4.3
PDGFS
IN
GLOMERULAR
CELLS
IN
VITRO
.
10
1.4.4
PDGFR-P
AND
ITS
LIGANDS
IN
RENAL
DISEASE
.
10
2.
MOTIVATION
AND
AIMS
.
12
3.
MATERIALSAND
METHODS
.
13
3.1
DEVICES
.
13
3.2
CONSUMABLES
.
14
3.3
REAGENTS
.
15
3.4
IN
VIVO
EXPERIMENTS
.
17
3.4.1
THIRTY-FIVE
WEEKS
OLD
THY
1.1
TRANSGENIC
MICE
.
17
3.4.2
MODELS
WITH
FSGS
.
17
3.4.3
NON-INVASIVE
BLOOD
PRESSURE
.
18
3.4.4
KIDNEY
HARVEST
AND
FIXATION
.
18
3.5
HUMAN
SAMPLES
.
19
3.6
HISTOLOGY
AND
MORPHOLOGY
.
19
3.6.1
PERIODIC
ACID
SCHIFF
(PAS)
.
20
3.6.2
IMMUNOHISTOCHEMISTRY
(IHC)
.
21
3.6.3
IMMUNOFLUORESCENCE
(IF)
.
24
3.6.4
FLUORESCENCE
IN
SITU
HYBRIDIZATION
(FISH)
.
24
3.7
IN
VITRO
CELL
CULTURE
EXPERIMENTS
.
26
3.7.1
THAWING
AND
CULTURE
OF
CELLS
.
26
3.7.2
CULTURE
&
SEEDING
OF
PECS
.
26
3.7.3
PECS
STIMULATION
.
27
3.7.4
PROLIFERATION
ASSAY
.
27
3.7.5 WOUND
HEALING
AND
MIGRATION
ASSAYS
.
29
3.8
PROTEIN
EXTRACTION
AND
ANALYSIS
.
30
3.8.1
PROTEIN
EXTRACTION
FROM
CELLS
.
30
3.8.2
SDS-PAGE
.
30
3.8.3
WESTERN
BLOT
.
31
3.9
MRNA
EXTRACTION
AND
ANALYSIS
.
32
3.9.1
RNA
EXTRACTION
FROM
CELLS
.
32
3.9.2
CDNA-SYNTHESIS
.
33
3.9.3
REAL-TIME
QUANTITATIVE
PCR
(RT-QPCR)
.
34
3.10
STATISTICAL
ANALYSIS
.
36
4.
RESULTS
.
37
4.1
SPONTANEOUS
PHENOTYPE
OF
35
WEEKS
OLD
THY1
.1
TRANSGENIC
MICE
.
37
4.2
CHARACTERIZATION
OF
THE
PROGRESSIVE
FSGS
THY1.1
MOUSE
MODEL
.
40
4.3 PDGFR-P
AND
ITS
LIGANDS
PDGF-B
AND
PDGF-D
WERE
UPREGULATED
IN
THE
FSGSTHY1.1
MOUSE
MODEL
.
50
4.4
KIDNEY
TUBULOINTERSTITIAL
FIBROSIS
WAS
INDUCED
IN
THY1
.1
FSGS
MODELS
.
55
4.5
PDGFR-P
AND
ITS
LIGANDS
PDGF-B
AND
PDGF-D
WERE
UPREGULATED
IN
HUMAN
FSGS
.
57
4.6
STIMULATION
OF
PRIMARY
MOUSE
PECS
.
59
4.6.1
TGF-PI
STIMULATION
IN
PRIMARY
PECS
.
59
4.6.2
PDGF-B
STIMULATION
IN
PRIMARY
PECS
.
62
4.6.3
PDGF-D
STIMULATION
IN
PRIMARY
PECS
.
64
5.
DISCUSSION
.
68
5.1
SPONTANEOUS
PHENOTYPE
OF
35
WEEKS
OLD
THY1.1
TRANSGENIC
MICE
.
68
5.2
CHARACTERIZATION
AND
MECHANISM
OF
THE
FSGS
THY1.1
MOUSE
MODEL
.69
5.3
PDGFR-P
AND
ITS
LIGANDS
PDGF-B
AND
PDGF-D
IN
FSGS
.
71
6.
SUMMARY
.
74
7.
ZUSAMMENFASSUNG
.
75
8.
REFERENCES
.
76
9.
PUBLICATION
.
83
10.
ACKNOWLEDGEMENT
.
84
11.
AFFIDAVITS
.
85
12.
CURRICULUM
VITAE
.
87 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV048191582 |
| illustrated | Illustrated |
| index_date | 2024-07-03T19:44:48Z |
| indexdate | 2024-07-10T09:31:34Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033572721 |
| oclc_num | 1314899313 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 87 Seiten Illustrationen, Diagramme 21 cm |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| record_format | marc |
| spelling | Jia, Ting 1988- Verfasser (DE-588)1238839797 aut PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) vorgelegt von Ting Jia Aachen [2021] 87 Seiten Illustrationen, Diagramme 21 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2021 (DE-588)4113937-9 Hochschulschrift gnd-content B:DE-101 application/pdf https://d-nb.info/1245041282/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033572721&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p emakn 0,13685 20220406 DE-101 https://d-nb.info/provenance/plan#emakn 2\p dnb 20220420 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Jia, Ting 1988- PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |
| subject_GND | (DE-588)4113937-9 |
| title | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |
| title_auth | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |
| title_exact_search | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |
| title_exact_search_txtP | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |
| title_full | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) vorgelegt von Ting Jia |
| title_fullStr | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) vorgelegt von Ting Jia |
| title_full_unstemmed | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) vorgelegt von Ting Jia |
| title_short | PDGFR-β and its ligands in focal segmental glomerulosclerosis (FSGS) |
| title_sort | pdgfr β and its ligands in focal segmental glomerulosclerosis fsgs |
| topic_facet | Hochschulschrift |
| url | https://d-nb.info/1245041282/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033572721&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT jiating pdgfrbanditsligandsinfocalsegmentalglomerulosclerosisfsgs |
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