Basic bioscience laboratory techniques: a pocket guide
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Hoboken, NJ
Wiley Blackwell
2022
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| Ausgabe: | Second edition |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xvii, 187 Seiten Illustrationen, Diagramme |
| ISBN: | 9781119663355 |
Internformat
MARC
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| 653 | 6 | |a Manuels de laboratoire | |
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Datensatz im Suchindex
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|---|---|
| adam_text | CONTENTS vii ix xiii xvii Preface Glossary Abbreviations About the Companion Website 1 The Preparation of Solutions in Bioscience Research 1.1 Introduction 1.2 Concentration 1.3 Using a Balance to Weigh Out Reagents 1.4 Practical Considerations When Making a 1,0M Solution 1.5 Dilutions and the Use of Pipettes 1.6 Water, Acids and Bases 1.7 Buffers 1.8 The Equilibrium/Dissociation Constant (řj for an Acid or Base and the Henderson-Hasselbalch Equation 1.9 Summary Notes 1 1 2 2 5 9 13 16 18 20 20 2 Microscopy 2.1 Introduction 2.2 Microscopes - General Principles 2.3 Principles of Image Formation 2.4 Light Microscopy 2.5 Electron Microscopy 2.6 Summary 21 21 22 23 25 36 39 3 Spectrophotometry 3.1 Introduction 3.2 The Electromagnetic Spectrum 3.3 The Absorbance of Light 3.4 Absorption Spectrophotometry 3.5 The Laws Governing the Absorbanceof Light 3.6 The Beer-Lambert Law 3.7 Spectrophotometers 3.8 Applications of Spectrophotometry inBioscience 3.9 Summary Notes 41 41 41 43 45 45 47 49 54 62 62 4 Data Analysis and Presentation 4.1 Introduction 4.2 Statistical Analysis of Data: Some Key Definitions 4.3 Distributions 4.4 Statistical Comparison of Data 4.5 Presentation, Structure and Organization of Data in Laboratory Reports 4.6 Summary 63 63 64 67 70 77 82 v
CONTENTS 5 The Extraction and Clarification of Biological Material 5.1 Introduction 5.2 Extraction 5.3 The Extraction Methods Used for Animal and Plant Tissue 5.4 The Extraction Methods for Bacteria 5.5 Clarification 5.6 Centrifugation Techniques 5.7 Points of Good Practice in Centrifugation 5.8 Summary Notes 83 83 83 84 86 87 92 93 94 95 6 Electrophoresis of Proteins and Nucleic Acids 6.1 General Introduction 6.2 Separation of Protein Mixtures by Gel Electrophoresis 6.3 Other Electrophoretic Techniques Applied to Proteins 6.4 Separation of Nucleic Acids by Gel Electrophoresis 6.5 Application^ of Gel Electrophoresis of Nucleic Acids 6.6 Summary 97 97 97 106 110 113 115 7 Chromatography 7.1 Introduction 7.2 The Theory of Chromatography 7.3 Factors to Consider in Chromatography 7.4 The Methods Used to Elute Samples in Chromatography 7.5 A Selection of Methods that Can be Used to Exchange Buffers and Concentrate Samples Prior to Chromatography 7.6 Vacuum Pumps 7.7 Different Tÿpes of Chromatography and What Properties Can be Used to Separate Molecules 7.8 Thin-layer Chromatography (TLC) 7.9 High-Pressure Liquid Chromatography (HPLC) 7.10 Gas/Liquid Chromatography (GLC) 7.11 Ion Exchange Chromatography (IEX) 7.12 Size Exclusion Chromatography (SEC) 7.13 Affinity Chromatography 7.14 Summary Notes 117 117 117 120 122 8 Cell Culture Techniques 8.1 Introduction 8.2 Growth and Maintenance of Cells in Culture 8.3 Summary Notes 141 141 145 157 157 9 Antibody-Based Assays (Immunoassays) 9.1 Antibody Structure and Uses 9.2 Antibody Purification, Labelling and Detection 9.3 Immunoassay
Methods 9.4 Controls 9.5 Summary 159 159 160 162 175 175 Suggestions for Further Reading Index 177 181 vi 123 127 128 130 131 133 134 136 138 139 139
|
| adam_txt |
CONTENTS vii ix xiii xvii Preface Glossary Abbreviations About the Companion Website 1 The Preparation of Solutions in Bioscience Research 1.1 Introduction 1.2 Concentration 1.3 Using a Balance to Weigh Out Reagents 1.4 Practical Considerations When Making a 1,0M Solution 1.5 Dilutions and the Use of Pipettes 1.6 Water, Acids and Bases 1.7 Buffers 1.8 The Equilibrium/Dissociation Constant (řj for an Acid or Base and the Henderson-Hasselbalch Equation 1.9 Summary Notes 1 1 2 2 5 9 13 16 18 20 20 2 Microscopy 2.1 Introduction 2.2 Microscopes - General Principles 2.3 Principles of Image Formation 2.4 Light Microscopy 2.5 Electron Microscopy 2.6 Summary 21 21 22 23 25 36 39 3 Spectrophotometry 3.1 Introduction 3.2 The Electromagnetic Spectrum 3.3 The Absorbance of Light 3.4 Absorption Spectrophotometry 3.5 The Laws Governing the Absorbanceof Light 3.6 The Beer-Lambert Law 3.7 Spectrophotometers 3.8 Applications of Spectrophotometry inBioscience 3.9 Summary Notes 41 41 41 43 45 45 47 49 54 62 62 4 Data Analysis and Presentation 4.1 Introduction 4.2 Statistical Analysis of Data: Some Key Definitions 4.3 Distributions 4.4 Statistical Comparison of Data 4.5 Presentation, Structure and Organization of Data in Laboratory Reports 4.6 Summary 63 63 64 67 70 77 82 v
CONTENTS 5 The Extraction and Clarification of Biological Material 5.1 Introduction 5.2 Extraction 5.3 The Extraction Methods Used for Animal and Plant Tissue 5.4 The Extraction Methods for Bacteria 5.5 Clarification 5.6 Centrifugation Techniques 5.7 Points of Good Practice in Centrifugation 5.8 Summary Notes 83 83 83 84 86 87 92 93 94 95 6 Electrophoresis of Proteins and Nucleic Acids 6.1 General Introduction 6.2 Separation of Protein Mixtures by Gel Electrophoresis 6.3 Other Electrophoretic Techniques Applied to Proteins 6.4 Separation of Nucleic Acids by Gel Electrophoresis 6.5 Application^ of Gel Electrophoresis of Nucleic Acids 6.6 Summary 97 97 97 106 110 113 115 7 Chromatography 7.1 Introduction 7.2 The Theory of Chromatography 7.3 Factors to Consider in Chromatography 7.4 The Methods Used to Elute Samples in Chromatography 7.5 A Selection of Methods that Can be Used to Exchange Buffers and Concentrate Samples Prior to Chromatography 7.6 Vacuum Pumps 7.7 Different Tÿpes of Chromatography and What Properties Can be Used to Separate Molecules 7.8 Thin-layer Chromatography (TLC) 7.9 High-Pressure Liquid Chromatography (HPLC) 7.10 Gas/Liquid Chromatography (GLC) 7.11 Ion Exchange Chromatography (IEX) 7.12 Size Exclusion Chromatography (SEC) 7.13 Affinity Chromatography 7.14 Summary Notes 117 117 117 120 122 8 Cell Culture Techniques 8.1 Introduction 8.2 Growth and Maintenance of Cells in Culture 8.3 Summary Notes 141 141 145 157 157 9 Antibody-Based Assays (Immunoassays) 9.1 Antibody Structure and Uses 9.2 Antibody Purification, Labelling and Detection 9.3 Immunoassay
Methods 9.4 Controls 9.5 Summary 159 159 160 162 175 175 Suggestions for Further Reading Index 177 181 vi 123 127 128 130 131 133 134 136 138 139 139 |
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| discipline | Biologie |
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| edition | Second edition |
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| index_date | 2024-07-03T19:39:20Z |
| indexdate | 2024-07-10T09:26:42Z |
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| isbn | 9781119663355 |
| language | English |
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| spelling | Bonner, Philip L. R. Verfasser (DE-588)1168596645 aut Basic bioscience laboratory techniques a pocket guide Philip L.R. Bonner and Alan J. Hargreaves Second edition Hoboken, NJ Wiley Blackwell 2022 xvii, 187 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Biowissenschaften (DE-588)4129772-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Biomedical Research Laboratory Manual Handbook Laboratory manuals Handbooks and manuals Manuels de laboratoire Guides et manuels Biowissenschaften (DE-588)4129772-6 s Labortechnik (DE-588)4123602-6 s DE-604 Hargreaves, Alan J. Verfasser (DE-588)1187865125 aut Erscheint auch als Online-Ausgabe, PDF 978-1-119-66344-7 Erscheint auch als Online-Ausgabe, EPUB 978-1-119-66348-5 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033338125&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bonner, Philip L. R. Hargreaves, Alan J. Basic bioscience laboratory techniques a pocket guide Biowissenschaften (DE-588)4129772-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4129772-6 (DE-588)4123602-6 |
| title | Basic bioscience laboratory techniques a pocket guide |
| title_auth | Basic bioscience laboratory techniques a pocket guide |
| title_exact_search | Basic bioscience laboratory techniques a pocket guide |
| title_exact_search_txtP | Basic bioscience laboratory techniques a pocket guide |
| title_full | Basic bioscience laboratory techniques a pocket guide Philip L.R. Bonner and Alan J. Hargreaves |
| title_fullStr | Basic bioscience laboratory techniques a pocket guide Philip L.R. Bonner and Alan J. Hargreaves |
| title_full_unstemmed | Basic bioscience laboratory techniques a pocket guide Philip L.R. Bonner and Alan J. Hargreaves |
| title_short | Basic bioscience laboratory techniques |
| title_sort | basic bioscience laboratory techniques a pocket guide |
| title_sub | a pocket guide |
| topic | Biowissenschaften (DE-588)4129772-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Biowissenschaften Labortechnik |
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