Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Gießen
VVB Laufersweiler Verlag
2020
|
| Edition: | 1. Auflage |
| Series: | Edition scientifique
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | iv, 123 Seiten Illustrationen, Diagramme 21 cm, 200 g |
| ISBN: | 9783835969018 3835969013 |
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MARC
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| 100 | 1 | |a Bulun, Hakan |e Verfasser |0 (DE-588)1222114712 |4 aut | |
| 245 | 1 | 0 | |a Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle |c eingereicht von Hakan Bulun |
| 250 | |a 1. Auflage | ||
| 264 | 1 | |a Gießen |b VVB Laufersweiler Verlag |c 2020 | |
| 300 | |a iv, 123 Seiten |b Illustrationen, Diagramme |c 21 cm, 200 g | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Edition scientifique | |
| 502 | |b Dissertation |c Justus-Liebig-Universität Gießen |d 2020 | ||
| 650 | 7 | |a cattle |2 cabt | |
| 650 | 7 | |a Mycobacterium avium ssp. paratuberculosis |2 cabt | |
| 650 | 7 | |a interferon-gamma |2 cabt | |
| 650 | 7 | |a paratubercolosis |2 cabt | |
| 650 | 7 | |a flow cytometry |2 cabt | |
| 650 | 7 | |a lymphocyte transformation tests |2 cabt | |
| 653 | |a Wissenschaft | ||
| 653 | |a Doktorarbeit | ||
| 653 | |a Uni | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 710 | 2 | |a VVB LAUFERSWEILER Verlag |0 (DE-588)1065553803 |4 pbl | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-033281251 | ||
| 883 | 2 | |8 1\p |a dnb |d 20220118 |q DE-101 |u https://d-nb.info/provenance/plan#dnb | |
Record in the Search Index
| _version_ | 1804183510255140864 |
|---|---|
| adam_text | CONTENTS
ABBREVIATIONS
......................................................................................................................................
I
PUBLICATIONS
1.
INTRODUCTION
..........................................................................................................................
1
2.
LITERATURE
REVIEW
................................................................................................................
3
2.1.
PARATUBERCULOSIS
IN
CATTLE
.......................................................................................................
4
2.2.
PARATUBERCULOSIS
IN
OTHER
RUMINANTS
.....................................................................................
7
2.3.
CROHN
S
DISEASE
AND
PARATUBERCULOSIS
..................................................................................
8
2.4.
DEVELOPMENT
OF
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
IN
CATTLE
.................................
9
2.5.
HUMORAL
IMMUNE
RESPONSE
IN
MAP-INFECTED
CATTLE
.........................................................
11
2.6.
DIAGNOSIS
OF
BOVINE
PARATUBERCULOSIS
.................................................................................
12
2.6.1.
ANTIGENS
USED
FOR
CELL-MEDIATED
IMMUNE
RESPONSE
(CMI)
BASED
DIAGNOSIS
OF
MAP
....
14
2.6.1.1.
ANTIGEN
MIXTURES
..................................................................................................................
14
2.6.1.2.
LIPID
ANTIGENS
.....................................................................................................................
15
2.6.1.3.
PROTEIN
ANTIGENS
....................................................................................................................
15
2.6.2.
DETECTION
OF
MAP-SPECIFIC
CMI
......................................................................................
18
2.6.2.1.
SKIN
TESTS
..............................................................................................................................
19
2.6.2.2.
LYMPHOCYTE
TRANSFORMATION
TEST
(LTT)
..............................................................................
20
2.6.2.3.
IFN-Y
RELEASE
ASSAY
(IGRA)
................................................................................................
21
2.6.2.4.
FLOW
CYTOMETRIC
ANALYSIS
(FCA)
........................................................................................
24
3.
MATERIAL
AND
METHODS
.......................................................................................................
25
3.1.
ANIMALS
................................................................................................................................
25
3.1.1.
MAP-NEGATIVE
AND
MAP-POSITIVE
HERDS
............................................................................
25
3.1.2.
COWS
SELECTED
FOR
THE
PRELIMINARY
STUDY
............................................................................
26
3.1.3.
CALVES
SELECTED
FOR
THE
LONGITUDINAL
STUDIES
......................................................................
27
3.2.
LONGITUDINAL
STUDY
DESIGN
...................................................................................................
30
3.3.
B
LOOD
SAMPLES
.....................................................................................................................
31
3.4.
ANTIGENS
...............................................................................................................................
32
3.4.1.
PREPARATION
OF
PURIFIED
PROTEIN
DERIVATIVES
(PPD)
.............................................................
33
3.4.2.
PREPARATION
OF
WHOLE
CELL
SONICATES
(WCS)
........................................................................
34
3.5.
INTERFERON
GAMMA
RELEASE
ASSAY
(IGRA)
............................................................................
34
3.5.1.
TEST
PRINCIPLE
........................................................................................................................
34
3.5.2.
TEST
PROCEDURE
......................................................................................................................
35
3.6.
IFN-Y
FLOW
CYTOMETRIC
ASSAY
(IFN-Y
FCA)
.......................................................................
36
3.6.1.
TEST
PRINCIPLE
........................................................................................................................
36
3.6.2.
ISOLATION
OF
BOVINE
PERIPHERAL
BLOOD
MONONUCLEAR
CELLS
(PBMC)
....................................
36
3.6.3.
ANTIGEN
STIMULATION
AND
BOOSTERING
OF
PBMC
CULTURES
.....................................................
37
CONTENTS
3.6.4.
DETECTION
OF
IFN-Y
PRODUCTION
IN
CD4
K
AND
CD8
+
PBMC
(2-COLOR
IMMUNOLABELING)
..
38
3.6.5.
FLOW
CYTOMETRIC
ANALYSIS
.....................................................................................................
39
3.7.
DATA
AND
STATISTICAL
ANALYSIS
.................................................................................................
42
3.7.1.
ANALYSIS
OF
IFN-Y
ELISA
....................................................................................................
42
3.7.2.
ANALYSIS
OF
IFN-Y
FLOW
CYTOMETRIC
ASSAY
...........................................................................
42
3.7.3.
ANALYSIS
OF
THE
SENSITIVITY
AND
THE
SPECIFICITY
OF
CMI
TESTS
IN
THE
LONGITUDINAL
STUDIES
.
43
4.
RESULTS
..................................................................................................................................
44
4.1.
COWS
UNEXPOSED
OR
NATURALLY
EXPOSED
TO
MYCOBACTERIUM
AVIUM
SSP.
PARATUBERCULOSIS
(MAP)
(PRELIMINARY
STUDY)
..................................................................................................44
4.1.1.
IFN-Y
PRODUCTION
IN
ANTIGEN-STIMULATED
WHOLE
BLOOD
CELL
CULTURES
(IGRA)
....................44
4.1.2.
ANTIGEN-INDUCED
IFN-Y
PRODUCTION
BY T-CELL
SUBPOPULATIONS
IN
PBMC
CULTURES
OF
MAP
INFECTED
CATTLE
(IFN-Y
FCA)
................................................................................................
45
4.2.
EXPERIMENTALLY
INFECTED
CALVES
(LONGITUDINAL
STUDIES)
.......................................................
61
4.2.1.
IFN-Y
RELEASE
IN
ANTIGEN-STIMULATED
WHOLE
BLOOD
CELL
CULTURES
(IGRA)
...........................
61
4.2.2.
IFN-Y
PRODUCTION
IN
ANTIGEN-STIMULATED
PBMC
CULTURES
(IFN-Y
FCA)
..........................
62
4.2.3.
COMPARISON
OF
MYCOBATERIAL
ANTIGEN
PREPARATIONS
TO
STIMULATE
PBMC
OF
EXPERIMENTALLY
INFECTED
CALVES
IN
VITRO
........................................................................................................
73
4.2.4.
SENSITIVITY
AND
SPECIFICITY
OF
CMI
TESTS
IN
THE
LONGITUDINAL
STUDIES
................................
75
5.
DISCUSSION
............................................................................................................................
82
5.1.
IFN-Y
RELEASE
ASSAY
FOLLOWING
ANTIGENIC
RESTIMULATION
OF
WHOLE
BLOOD
CELL
CULTURES
82
5.2.
RATIONALE
FOR
THE
SELECTION
OF
ANTIGENS
TO
DETECT
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
BY
FLOW
CYTOMETRIC
ANALYSIS
.................................................................................................
85
5.3.
AMENDMENT
OF
A
FLOW
CYTOMETRIC
ANALYSIS
PROTOCOL
TO
DETECT
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
...............................................................................................................
87
5.4.
CHOOSING
A
SUITABLE
EXPERIMENTAL
MODEL
TO
QUANTIFY
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
IN
INFECTED
CALVES
................................................................................................
90
5.5.
ASSESSMENT
OF
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
AS
EARLY
DIAGNOSTIC
MARKER
IN
EXPERIMENTALLY
INFECTED
CALVES
...........................................................................................
91
5.6.
TRANSLATIONAL
POTENTIAL
OF
THE
STUDY
RESULTS
AND
OUTLOOK
......................................................
93
6.
CONCLUSIONS
.........................................................................................................................
94
7.
SUMMARY
.............................................................................................................................
95
8.
ZUSAMMENFASSUNG
.............................................................................................................
96
9.
REFERENCES
...........................................................................................................................
97
10.
APPENDIX
...........................................................................................................................
112
10.1.
REAGENTS
AND
MATERIALS
......................................................................................................
112
10.2.
LABORATORY
EQUIPMENTS
......................................................................................................
115
CONTENTS
10.3.
BUFFERS,
SOLUTIONS
AND
CULTURE
MEDIA
.................................................................................
116
10.4.
TEST
KITS
..............................................................................................................................
119
LIST
OF
TABLES
.................................................................................................................................
120
LIST
OF
FIGURES
................................................................................................................................
121
ACKNOWLEDGMENT
...........................................................................................................................
123
|
| adam_txt |
CONTENTS
ABBREVIATIONS
.
I
PUBLICATIONS
1.
INTRODUCTION
.
1
2.
LITERATURE
REVIEW
.
3
2.1.
PARATUBERCULOSIS
IN
CATTLE
.
4
2.2.
PARATUBERCULOSIS
IN
OTHER
RUMINANTS
.
7
2.3.
CROHN
'
S
DISEASE
AND
PARATUBERCULOSIS
.
8
2.4.
DEVELOPMENT
OF
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
IN
CATTLE
.
9
2.5.
HUMORAL
IMMUNE
RESPONSE
IN
MAP-INFECTED
CATTLE
.
11
2.6.
DIAGNOSIS
OF
BOVINE
PARATUBERCULOSIS
.
12
2.6.1.
ANTIGENS
USED
FOR
CELL-MEDIATED
IMMUNE
RESPONSE
(CMI)
BASED
DIAGNOSIS
OF
MAP
.
14
2.6.1.1.
ANTIGEN
MIXTURES
.
14
2.6.1.2.
LIPID
ANTIGENS
.
15
2.6.1.3.
PROTEIN
ANTIGENS
.
15
2.6.2.
DETECTION
OF
MAP-SPECIFIC
CMI
.
18
2.6.2.1.
SKIN
TESTS
.
19
2.6.2.2.
LYMPHOCYTE
TRANSFORMATION
TEST
(LTT)
.
20
2.6.2.3.
IFN-Y
RELEASE
ASSAY
(IGRA)
.
21
2.6.2.4.
FLOW
CYTOMETRIC
ANALYSIS
(FCA)
.
24
3.
MATERIAL
AND
METHODS
.
25
3.1.
ANIMALS
.
25
3.1.1.
MAP-NEGATIVE
AND
MAP-POSITIVE
HERDS
.
25
3.1.2.
COWS
SELECTED
FOR
THE
PRELIMINARY
STUDY
.
26
3.1.3.
CALVES
SELECTED
FOR
THE
LONGITUDINAL
STUDIES
.
27
3.2.
LONGITUDINAL
STUDY
DESIGN
.
30
3.3.
B
LOOD
SAMPLES
.
31
3.4.
ANTIGENS
.
32
3.4.1.
PREPARATION
OF
PURIFIED
PROTEIN
DERIVATIVES
(PPD)
.
33
3.4.2.
PREPARATION
OF
WHOLE
CELL
SONICATES
(WCS)
.
34
3.5.
INTERFERON
GAMMA
RELEASE
ASSAY
(IGRA)
.
34
3.5.1.
TEST
PRINCIPLE
.
34
3.5.2.
TEST
PROCEDURE
.
35
3.6.
IFN-Y
FLOW
CYTOMETRIC
ASSAY
(IFN-Y
FCA)
.
36
3.6.1.
TEST
PRINCIPLE
.
36
3.6.2.
ISOLATION
OF
BOVINE
PERIPHERAL
BLOOD
MONONUCLEAR
CELLS
(PBMC)
.
36
3.6.3.
ANTIGEN
STIMULATION
AND
BOOSTERING
OF
PBMC
CULTURES
.
37
CONTENTS
3.6.4.
DETECTION
OF
IFN-Y
PRODUCTION
IN
CD4
K
AND
CD8
+
PBMC
(2-COLOR
IMMUNOLABELING)
.
38
3.6.5.
FLOW
CYTOMETRIC
ANALYSIS
.
39
3.7.
DATA
AND
STATISTICAL
ANALYSIS
.
42
3.7.1.
ANALYSIS
OF
IFN-Y
ELISA
.
42
3.7.2.
ANALYSIS
OF
IFN-Y
FLOW
CYTOMETRIC
ASSAY
.
42
3.7.3.
ANALYSIS
OF
THE
SENSITIVITY
AND
THE
SPECIFICITY
OF
CMI
TESTS
IN
THE
LONGITUDINAL
STUDIES
.
43
4.
RESULTS
.
44
4.1.
COWS
UNEXPOSED
OR
NATURALLY
EXPOSED
TO
MYCOBACTERIUM
AVIUM
SSP.
PARATUBERCULOSIS
(MAP)
(PRELIMINARY
STUDY)
.44
4.1.1.
IFN-Y
PRODUCTION
IN
ANTIGEN-STIMULATED
WHOLE
BLOOD
CELL
CULTURES
(IGRA)
.44
4.1.2.
ANTIGEN-INDUCED
IFN-Y
PRODUCTION
BY T-CELL
SUBPOPULATIONS
IN
PBMC
CULTURES
OF
MAP
INFECTED
CATTLE
(IFN-Y
FCA)
.
45
4.2.
EXPERIMENTALLY
INFECTED
CALVES
(LONGITUDINAL
STUDIES)
.
61
4.2.1.
IFN-Y
RELEASE
IN
ANTIGEN-STIMULATED
WHOLE
BLOOD
CELL
CULTURES
(IGRA)
.
61
4.2.2.
IFN-Y
PRODUCTION
IN
ANTIGEN-STIMULATED
PBMC
CULTURES
(IFN-Y
FCA)
.
62
4.2.3.
COMPARISON
OF
MYCOBATERIAL
ANTIGEN
PREPARATIONS
TO
STIMULATE
PBMC
OF
EXPERIMENTALLY
INFECTED
CALVES
IN
VITRO
.
73
4.2.4.
SENSITIVITY
AND
SPECIFICITY
OF
CMI
TESTS
IN
THE
LONGITUDINAL
STUDIES
.
75
5.
DISCUSSION
.
82
5.1.
IFN-Y
RELEASE
ASSAY
FOLLOWING
ANTIGENIC
RESTIMULATION
OF
WHOLE
BLOOD
CELL
CULTURES
82
5.2.
RATIONALE
FOR
THE
SELECTION
OF
ANTIGENS
TO
DETECT
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
BY
FLOW
CYTOMETRIC
ANALYSIS
.
85
5.3.
AMENDMENT
OF
A
FLOW
CYTOMETRIC
ANALYSIS
PROTOCOL
TO
DETECT
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
.
87
5.4.
CHOOSING
A
SUITABLE
EXPERIMENTAL
MODEL
TO
QUANTIFY
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
IN
INFECTED
CALVES
.
90
5.5.
ASSESSMENT
OF
MAP-SPECIFIC
CELLULAR
IMMUNE
RESPONSES
AS
EARLY
DIAGNOSTIC
MARKER
IN
EXPERIMENTALLY
INFECTED
CALVES
.
91
5.6.
TRANSLATIONAL
POTENTIAL
OF
THE
STUDY
RESULTS
AND
OUTLOOK
.
93
6.
CONCLUSIONS
.
94
7.
SUMMARY
.
95
8.
ZUSAMMENFASSUNG
.
96
9.
REFERENCES
.
97
10.
APPENDIX
.
112
10.1.
REAGENTS
AND
MATERIALS
.
112
10.2.
LABORATORY
EQUIPMENTS
.
115
CONTENTS
10.3.
BUFFERS,
SOLUTIONS
AND
CULTURE
MEDIA
.
116
10.4.
TEST
KITS
.
119
LIST
OF
TABLES
.
120
LIST
OF
FIGURES
.
121
ACKNOWLEDGMENT
.
123 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Bulun, Hakan |
| author_GND | (DE-588)1222114712 |
| author_facet | Bulun, Hakan |
| author_role | aut |
| author_sort | Bulun, Hakan |
| author_variant | h b hb |
| building | Verbundindex |
| bvnumber | BV047899299 |
| ctrlnum | (OCoLC)1224153732 (DE-599)DNB1221629344 |
| edition | 1. Auflage |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047899299 |
| illustrated | Illustrated |
| index_date | 2024-07-03T19:28:27Z |
| indexdate | 2024-07-10T09:24:35Z |
| institution | BVB |
| institution_GND | (DE-588)1065553803 |
| isbn | 9783835969018 3835969013 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033281251 |
| oclc_num | 1224153732 |
| open_access_boolean | |
| owner | DE-188 |
| owner_facet | DE-188 |
| physical | iv, 123 Seiten Illustrationen, Diagramme 21 cm, 200 g |
| publishDate | 2020 |
| publishDateSearch | 2020 |
| publishDateSort | 2020 |
| publisher | VVB Laufersweiler Verlag |
| record_format | marc |
| series2 | Edition scientifique |
| spelling | Bulun, Hakan Verfasser (DE-588)1222114712 aut Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle eingereicht von Hakan Bulun 1. Auflage Gießen VVB Laufersweiler Verlag 2020 iv, 123 Seiten Illustrationen, Diagramme 21 cm, 200 g txt rdacontent n rdamedia nc rdacarrier Edition scientifique Dissertation Justus-Liebig-Universität Gießen 2020 cattle cabt Mycobacterium avium ssp. paratuberculosis cabt interferon-gamma cabt paratubercolosis cabt flow cytometry cabt lymphocyte transformation tests cabt Wissenschaft Doktorarbeit Uni (DE-588)4113937-9 Hochschulschrift gnd-content VVB LAUFERSWEILER Verlag (DE-588)1065553803 pbl B:DE-101 application/pdf https://d-nb.info/1221629344/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033281251&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p dnb 20220118 DE-101 https://d-nb.info/provenance/plan#dnb |
| spellingShingle | Bulun, Hakan Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle cattle cabt Mycobacterium avium ssp. paratuberculosis cabt interferon-gamma cabt paratubercolosis cabt flow cytometry cabt lymphocyte transformation tests cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle |
| title_auth | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle |
| title_exact_search | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle |
| title_exact_search_txtP | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle |
| title_full | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle eingereicht von Hakan Bulun |
| title_fullStr | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle eingereicht von Hakan Bulun |
| title_full_unstemmed | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle eingereicht von Hakan Bulun |
| title_short | Interferon-gamma producing T-cells as an early diagnostic marker for Mycobacterium avium ssp. paratuberculosis infection in cattle |
| title_sort | interferon gamma producing t cells as an early diagnostic marker for mycobacterium avium ssp paratuberculosis infection in cattle |
| topic | cattle cabt Mycobacterium avium ssp. paratuberculosis cabt interferon-gamma cabt paratubercolosis cabt flow cytometry cabt lymphocyte transformation tests cabt |
| topic_facet | cattle Mycobacterium avium ssp. paratuberculosis interferon-gamma paratubercolosis flow cytometry lymphocyte transformation tests Hochschulschrift |
| url | https://d-nb.info/1221629344/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033281251&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bulunhakan interferongammaproducingtcellsasanearlydiagnosticmarkerformycobacteriumaviumsspparatuberculosisinfectionincattle AT vvblaufersweilerverlag interferongammaproducingtcellsasanearlydiagnosticmarkerformycobacteriumaviumsspparatuberculosisinfectionincattle |
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