Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging:
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
Jena
2021
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Physical Description: | 126 Seiten Illustrationen, Diagramme 29,5 cm |
Staff View
MARC
| LEADER | 00000nam a22000008c 4500 | ||
|---|---|---|---|
| 001 | BV047884251 | ||
| 003 | DE-604 | ||
| 005 | 20220401 | ||
| 007 | t | ||
| 008 | 220316s2021 gw a||| m||| 00||| eng d | ||
| 016 | 7 | |a 1247774708 |2 DE-101 | |
| 020 | |c Broschur | ||
| 035 | |a (OCoLC)1310255142 | ||
| 035 | |a (DE-599)DNB1247774708 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE | ||
| 049 | |a DE-355 | ||
| 084 | |a YB 5004 |0 (DE-625)153135:12910 |2 rvk | ||
| 100 | 1 | |a Rasa, Seyed Mohammad Mahdi |d 1989- |e Verfasser |0 (DE-588)1241283192 |4 aut | |
| 245 | 1 | 0 | |a Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |c by M. Sc., Seyed Mohammad Mahdi Rasa |
| 264 | 1 | |a Jena |c 2021 | |
| 300 | |a 126 Seiten |b Illustrationen, Diagramme |c 29,5 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |b Dissertation |c Friedrich-Schiller-Universität Jena |d 2021 | ||
| 650 | 0 | 7 | |a Altern |0 (DE-588)4068596-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Transkriptom |0 (DE-588)4775955-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikrobiom |g Genetik |0 (DE-588)1111812888 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Altern |0 (DE-588)4068596-2 |D s |
| 689 | 0 | 1 | |a Transkriptom |0 (DE-588)4775955-0 |D s |
| 689 | 0 | 2 | |a Mikrobiom |g Genetik |0 (DE-588)1111812888 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m B:DE-101 |q application/pdf |u https://d-nb.info/1247774708/04 |3 Inhaltsverzeichnis |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033266516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033266516 | ||
Record in the Search Index
| _version_ | 1804183484621651968 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
1.1.
ABSTRACT.
............................................................................................................................................
5
1.2.
ZUSAMMENFASSUNG
........................................................................................................................
6
1.3.
ABBREVIATIONS
..................................................................................................................................
7
2.
INTRODUCTION
....................................................................................................................................
9
2.1.
THE
IMPORTANCE
OF
AGING
RESEARCH
IN
MODERN
LIFE
..................................................................
9
2.2.
DIETARY
RESTRICTION
IS
THE
BEST
KNOWN
NON-INVASIVE
ANTI-AGING
TREATMENT
......................
10
2.3.
INFLAMMAGING
..............................................................................................................................
11
2.4.
MITOCHONDRIAL
DYSFUNCTION,
METABOLIC
AND
OXIDATIVE
STRESS..............................................13
2.5.
DNA
DAMAGE
...............................................................................................................................
15
2.6.
AUTOPHAGY
..................................................................................................................................
15
2.7.
CELLULAR
SENESCENCE
...................................................................................................................16
2.8.
STEM
CELL
FUNCTION,
TISSUE
REPAIR,
AND
REGENERATION
............................................................17
2.9.
MICROBIOME
DYSBIOSIS
AND
BARRIER
DYSFUNCTION
....................................................................18
3.
MATERIAL
AND
METHODS
.............................................................................................................
21
3.1.
MICE
..............................................................................................................................................21
3.2.
DIETARY
RESTRICTION
(DR)
...........................................................................................................21
3.3.
SAMPLE
COLLECTION
(FOR
RNA-SEQ
AND
ATAC-SEQ
STUDY)
.......................................................21
3.4.
TOTAL
RNA
SEQUENCING
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
............................................
22
3.5.
REVERSE
TRANSCRIPTION
AND
RT-QPCR
(FOR
GENE
EXPRESSION)
................................................
24
3.6.
MOUSE
SERUM
COLLECTION
AND
IFNG
(INTERFERON-GAMMA)
ELISA
DETECTION
..........................
25
3.7.
PREPARATION
OF
RECOMBINANT
TN5
TRANSPOSASE
......................................................................
26
3.8.
ATAC-SEQ
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
..................................................................
27
3.9.
SAMPLE
COLLECTION
FOR
MICROBIOME
STUDY
...............................................................................
28
3.10.
DNA
ISOLATION
AND
LIBRARY
PREPARATION
FOR
MICROBIOME
STUDY
..........................................29
3.11.
RT-QPCR
(FOR
MICROBIOME
STUDY)
............................................................................................32
3.12.
16S
RRNA
GENE
SEQUENCING
DATA
ANALYSIS
...............................................................................
33
4.
RESULTS
..............................................................................................................................................
39
.4.1
DR
PARTIALLY
RESCUES
THE
OLD
TRANSCRIPTOME
IN
MOST
OF
THE
TISSUES
...................................
39
4.2.
MOLECULAR
PATHWAYS
ALTERED
BY
AGING
AND
AMELIORATED
BY
LTDR
AND/OR
STDR
TREATMENTS
44
4.3.
A
MULTI-TISSUE
MOLECULAR
NETWORK
REGULATES
THE
INFLAMMATION
IN
AGING
AND
DR
...........
47
4.4.
INNATE
IMMUNE
SYSTEM
RECEPTORS
TRIGGER
THE
INFLAMMATORY
NETWORK
IN
AGING
THAT
IS
RESCUED
BY
DR
.....................................................................................................................................................
52
4.5.
DR
INHIBITS
THE
AGE-INDUCED
INTERFERON
RESPONSE
.................................................................
55
4.6.
AGING
INDUCES
A
GENERAL
OPEN
CHROMATIN
REARRANGEMENT
WHICH
IS
NOT
RESCUED
BY
DR,
EXCEPT
FOR
STATL
AND
IRF
TARGET
GENES
.............................................................................................................
64
4.7.
GUT
BACTERIAL
LOAD
AND
COMPOSITION
DEPENDS
ON
AGE,
INTESTINAL
PART
AND
GENDER
........
78
4.8.
GUT
MICROBIOME
DYSBIOSIS
IS
ASSOCIATED
WITH
THE
INFLAMMAGING
......................................
90
5.
DISCUSSION
....................................................................................................................................
102
6.
REFERENCE
....................................................................................................................................
112
|
| adam_txt |
TABLE
OF
CONTENTS
1.1.
ABSTRACT.
.
5
1.2.
ZUSAMMENFASSUNG
.
6
1.3.
ABBREVIATIONS
.
7
2.
INTRODUCTION
.
9
2.1.
THE
IMPORTANCE
OF
AGING
RESEARCH
IN
MODERN
LIFE
.
9
2.2.
DIETARY
RESTRICTION
IS
THE
BEST
KNOWN
NON-INVASIVE
ANTI-AGING
TREATMENT
.
10
2.3.
INFLAMMAGING
.
11
2.4.
MITOCHONDRIAL
DYSFUNCTION,
METABOLIC
AND
OXIDATIVE
STRESS.13
2.5.
DNA
DAMAGE
.
15
2.6.
AUTOPHAGY
.
15
2.7.
CELLULAR
SENESCENCE
.16
2.8.
STEM
CELL
FUNCTION,
TISSUE
REPAIR,
AND
REGENERATION
.17
2.9.
MICROBIOME
DYSBIOSIS
AND
BARRIER
DYSFUNCTION
.18
3.
MATERIAL
AND
METHODS
.
21
3.1.
MICE
.21
3.2.
DIETARY
RESTRICTION
(DR)
.21
3.3.
SAMPLE
COLLECTION
(FOR
RNA-SEQ
AND
ATAC-SEQ
STUDY)
.21
3.4.
TOTAL
RNA
SEQUENCING
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
.
22
3.5.
REVERSE
TRANSCRIPTION
AND
RT-QPCR
(FOR
GENE
EXPRESSION)
.
24
3.6.
MOUSE
SERUM
COLLECTION
AND
IFNG
(INTERFERON-GAMMA)
ELISA
DETECTION
.
25
3.7.
PREPARATION
OF
RECOMBINANT
TN5
TRANSPOSASE
.
26
3.8.
ATAC-SEQ
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
.
27
3.9.
SAMPLE
COLLECTION
FOR
MICROBIOME
STUDY
.
28
3.10.
DNA
ISOLATION
AND
LIBRARY
PREPARATION
FOR
MICROBIOME
STUDY
.29
3.11.
RT-QPCR
(FOR
MICROBIOME
STUDY)
.32
3.12.
16S
RRNA
GENE
SEQUENCING
DATA
ANALYSIS
.
33
4.
RESULTS
.
39
.4.1
DR
PARTIALLY
RESCUES
THE
OLD
TRANSCRIPTOME
IN
MOST
OF
THE
TISSUES
.
39
4.2.
MOLECULAR
PATHWAYS
ALTERED
BY
AGING
AND
AMELIORATED
BY
LTDR
AND/OR
STDR
TREATMENTS
44
4.3.
A
MULTI-TISSUE
MOLECULAR
NETWORK
REGULATES
THE
INFLAMMATION
IN
AGING
AND
DR
.
47
4.4.
INNATE
IMMUNE
SYSTEM
RECEPTORS
TRIGGER
THE
INFLAMMATORY
NETWORK
IN
AGING
THAT
IS
RESCUED
BY
DR
.
52
4.5.
DR
INHIBITS
THE
AGE-INDUCED
INTERFERON
RESPONSE
.
55
4.6.
AGING
INDUCES
A
GENERAL
OPEN
CHROMATIN
REARRANGEMENT
WHICH
IS
NOT
RESCUED
BY
DR,
EXCEPT
FOR
STATL
AND
IRF
TARGET
GENES
.
64
4.7.
GUT
BACTERIAL
LOAD
AND
COMPOSITION
DEPENDS
ON
AGE,
INTESTINAL
PART
AND
GENDER
.
78
4.8.
GUT
MICROBIOME
DYSBIOSIS
IS
ASSOCIATED
WITH
THE
INFLAMMAGING
.
90
5.
DISCUSSION
.
102
6.
REFERENCE
.
112 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Rasa, Seyed Mohammad Mahdi 1989- |
| author_GND | (DE-588)1241283192 |
| author_facet | Rasa, Seyed Mohammad Mahdi 1989- |
| author_role | aut |
| author_sort | Rasa, Seyed Mohammad Mahdi 1989- |
| author_variant | s m m r smm smmr |
| building | Verbundindex |
| bvnumber | BV047884251 |
| classification_rvk | YB 5004 |
| ctrlnum | (OCoLC)1310255142 (DE-599)DNB1247774708 |
| discipline | Medizin |
| discipline_str_mv | Medizin |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01801nam a22004218c 4500</leader><controlfield tag="001">BV047884251</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20220401 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">220316s2021 gw a||| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">1247774708</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="c">Broschur</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1310255142</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB1247774708</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">XA-DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">YB 5004</subfield><subfield code="0">(DE-625)153135:12910</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Rasa, Seyed Mohammad Mahdi</subfield><subfield code="d">1989-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)1241283192</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging</subfield><subfield code="c">by M. Sc., Seyed Mohammad Mahdi Rasa</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Jena</subfield><subfield code="c">2021</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">126 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">29,5 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="b">Dissertation</subfield><subfield code="c">Friedrich-Schiller-Universität Jena</subfield><subfield code="d">2021</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Altern</subfield><subfield code="0">(DE-588)4068596-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Transkriptom</subfield><subfield code="0">(DE-588)4775955-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mikrobiom</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)1111812888</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Altern</subfield><subfield code="0">(DE-588)4068596-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Transkriptom</subfield><subfield code="0">(DE-588)4775955-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Mikrobiom</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)1111812888</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">B:DE-101</subfield><subfield code="q">application/pdf</subfield><subfield code="u">https://d-nb.info/1247774708/04</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033266516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033266516</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV047884251 |
| illustrated | Illustrated |
| index_date | 2024-07-03T19:23:53Z |
| indexdate | 2024-07-10T09:24:11Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033266516 |
| oclc_num | 1310255142 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 126 Seiten Illustrationen, Diagramme 29,5 cm |
| publishDate | 2021 |
| publishDateSearch | 2021 |
| publishDateSort | 2021 |
| record_format | marc |
| spelling | Rasa, Seyed Mohammad Mahdi 1989- Verfasser (DE-588)1241283192 aut Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa Jena 2021 126 Seiten Illustrationen, Diagramme 29,5 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Schiller-Universität Jena 2021 Altern (DE-588)4068596-2 gnd rswk-swf Transkriptom (DE-588)4775955-0 gnd rswk-swf Mikrobiom Genetik (DE-588)1111812888 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Altern (DE-588)4068596-2 s Transkriptom (DE-588)4775955-0 s Mikrobiom Genetik (DE-588)1111812888 s DE-604 B:DE-101 application/pdf https://d-nb.info/1247774708/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033266516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rasa, Seyed Mohammad Mahdi 1989- Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging Altern (DE-588)4068596-2 gnd Transkriptom (DE-588)4775955-0 gnd Mikrobiom Genetik (DE-588)1111812888 gnd |
| subject_GND | (DE-588)4068596-2 (DE-588)4775955-0 (DE-588)1111812888 (DE-588)4113937-9 |
| title | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_auth | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_exact_search | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_exact_search_txtP | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_full | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa |
| title_fullStr | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa |
| title_full_unstemmed | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa |
| title_short | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_sort | characterization of the transcriptome the epigenome and the microbiome during aging dietary restriction ameliorates inflammaging |
| topic | Altern (DE-588)4068596-2 gnd Transkriptom (DE-588)4775955-0 gnd Mikrobiom Genetik (DE-588)1111812888 gnd |
| topic_facet | Altern Transkriptom Mikrobiom Genetik Hochschulschrift |
| url | https://d-nb.info/1247774708/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033266516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rasaseyedmohammadmahdi characterizationofthetranscriptometheepigenomeandthemicrobiomeduringagingdietaryrestrictionamelioratesinflammaging |
There is no print copy available.
Indexes