Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Jena
2021
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | 126 Seiten Illustrationen, Diagramme 29,5 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |c by M. Sc., Seyed Mohammad Mahdi Rasa |
| 264 | 1 | |a Jena |c 2021 | |
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| 502 | |b Dissertation |c Friedrich-Schiller-Universität Jena |d 2021 | ||
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Datensatz im Suchindex
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| adam_text | TABLE
OF
CONTENTS
1.1.
ABSTRACT.
............................................................................................................................................
5
1.2.
ZUSAMMENFASSUNG
........................................................................................................................
6
1.3.
ABBREVIATIONS
..................................................................................................................................
7
2.
INTRODUCTION
....................................................................................................................................
9
2.1.
THE
IMPORTANCE
OF
AGING
RESEARCH
IN
MODERN
LIFE
..................................................................
9
2.2.
DIETARY
RESTRICTION
IS
THE
BEST
KNOWN
NON-INVASIVE
ANTI-AGING
TREATMENT
......................
10
2.3.
INFLAMMAGING
..............................................................................................................................
11
2.4.
MITOCHONDRIAL
DYSFUNCTION,
METABOLIC
AND
OXIDATIVE
STRESS..............................................13
2.5.
DNA
DAMAGE
...............................................................................................................................
15
2.6.
AUTOPHAGY
..................................................................................................................................
15
2.7.
CELLULAR
SENESCENCE
...................................................................................................................16
2.8.
STEM
CELL
FUNCTION,
TISSUE
REPAIR,
AND
REGENERATION
............................................................17
2.9.
MICROBIOME
DYSBIOSIS
AND
BARRIER
DYSFUNCTION
....................................................................18
3.
MATERIAL
AND
METHODS
.............................................................................................................
21
3.1.
MICE
..............................................................................................................................................21
3.2.
DIETARY
RESTRICTION
(DR)
...........................................................................................................21
3.3.
SAMPLE
COLLECTION
(FOR
RNA-SEQ
AND
ATAC-SEQ
STUDY)
.......................................................21
3.4.
TOTAL
RNA
SEQUENCING
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
............................................
22
3.5.
REVERSE
TRANSCRIPTION
AND
RT-QPCR
(FOR
GENE
EXPRESSION)
................................................
24
3.6.
MOUSE
SERUM
COLLECTION
AND
IFNG
(INTERFERON-GAMMA)
ELISA
DETECTION
..........................
25
3.7.
PREPARATION
OF
RECOMBINANT
TN5
TRANSPOSASE
......................................................................
26
3.8.
ATAC-SEQ
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
..................................................................
27
3.9.
SAMPLE
COLLECTION
FOR
MICROBIOME
STUDY
...............................................................................
28
3.10.
DNA
ISOLATION
AND
LIBRARY
PREPARATION
FOR
MICROBIOME
STUDY
..........................................29
3.11.
RT-QPCR
(FOR
MICROBIOME
STUDY)
............................................................................................32
3.12.
16S
RRNA
GENE
SEQUENCING
DATA
ANALYSIS
...............................................................................
33
4.
RESULTS
..............................................................................................................................................
39
.4.1
DR
PARTIALLY
RESCUES
THE
OLD
TRANSCRIPTOME
IN
MOST
OF
THE
TISSUES
...................................
39
4.2.
MOLECULAR
PATHWAYS
ALTERED
BY
AGING
AND
AMELIORATED
BY
LTDR
AND/OR
STDR
TREATMENTS
44
4.3.
A
MULTI-TISSUE
MOLECULAR
NETWORK
REGULATES
THE
INFLAMMATION
IN
AGING
AND
DR
...........
47
4.4.
INNATE
IMMUNE
SYSTEM
RECEPTORS
TRIGGER
THE
INFLAMMATORY
NETWORK
IN
AGING
THAT
IS
RESCUED
BY
DR
.....................................................................................................................................................
52
4.5.
DR
INHIBITS
THE
AGE-INDUCED
INTERFERON
RESPONSE
.................................................................
55
4.6.
AGING
INDUCES
A
GENERAL
OPEN
CHROMATIN
REARRANGEMENT
WHICH
IS
NOT
RESCUED
BY
DR,
EXCEPT
FOR
STATL
AND
IRF
TARGET
GENES
.............................................................................................................
64
4.7.
GUT
BACTERIAL
LOAD
AND
COMPOSITION
DEPENDS
ON
AGE,
INTESTINAL
PART
AND
GENDER
........
78
4.8.
GUT
MICROBIOME
DYSBIOSIS
IS
ASSOCIATED
WITH
THE
INFLAMMAGING
......................................
90
5.
DISCUSSION
....................................................................................................................................
102
6.
REFERENCE
....................................................................................................................................
112
|
| adam_txt |
TABLE
OF
CONTENTS
1.1.
ABSTRACT.
.
5
1.2.
ZUSAMMENFASSUNG
.
6
1.3.
ABBREVIATIONS
.
7
2.
INTRODUCTION
.
9
2.1.
THE
IMPORTANCE
OF
AGING
RESEARCH
IN
MODERN
LIFE
.
9
2.2.
DIETARY
RESTRICTION
IS
THE
BEST
KNOWN
NON-INVASIVE
ANTI-AGING
TREATMENT
.
10
2.3.
INFLAMMAGING
.
11
2.4.
MITOCHONDRIAL
DYSFUNCTION,
METABOLIC
AND
OXIDATIVE
STRESS.13
2.5.
DNA
DAMAGE
.
15
2.6.
AUTOPHAGY
.
15
2.7.
CELLULAR
SENESCENCE
.16
2.8.
STEM
CELL
FUNCTION,
TISSUE
REPAIR,
AND
REGENERATION
.17
2.9.
MICROBIOME
DYSBIOSIS
AND
BARRIER
DYSFUNCTION
.18
3.
MATERIAL
AND
METHODS
.
21
3.1.
MICE
.21
3.2.
DIETARY
RESTRICTION
(DR)
.21
3.3.
SAMPLE
COLLECTION
(FOR
RNA-SEQ
AND
ATAC-SEQ
STUDY)
.21
3.4.
TOTAL
RNA
SEQUENCING
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
.
22
3.5.
REVERSE
TRANSCRIPTION
AND
RT-QPCR
(FOR
GENE
EXPRESSION)
.
24
3.6.
MOUSE
SERUM
COLLECTION
AND
IFNG
(INTERFERON-GAMMA)
ELISA
DETECTION
.
25
3.7.
PREPARATION
OF
RECOMBINANT
TN5
TRANSPOSASE
.
26
3.8.
ATAC-SEQ
LIBRARY
PREPARATION
AND
DATA
ANALYSIS
.
27
3.9.
SAMPLE
COLLECTION
FOR
MICROBIOME
STUDY
.
28
3.10.
DNA
ISOLATION
AND
LIBRARY
PREPARATION
FOR
MICROBIOME
STUDY
.29
3.11.
RT-QPCR
(FOR
MICROBIOME
STUDY)
.32
3.12.
16S
RRNA
GENE
SEQUENCING
DATA
ANALYSIS
.
33
4.
RESULTS
.
39
.4.1
DR
PARTIALLY
RESCUES
THE
OLD
TRANSCRIPTOME
IN
MOST
OF
THE
TISSUES
.
39
4.2.
MOLECULAR
PATHWAYS
ALTERED
BY
AGING
AND
AMELIORATED
BY
LTDR
AND/OR
STDR
TREATMENTS
44
4.3.
A
MULTI-TISSUE
MOLECULAR
NETWORK
REGULATES
THE
INFLAMMATION
IN
AGING
AND
DR
.
47
4.4.
INNATE
IMMUNE
SYSTEM
RECEPTORS
TRIGGER
THE
INFLAMMATORY
NETWORK
IN
AGING
THAT
IS
RESCUED
BY
DR
.
52
4.5.
DR
INHIBITS
THE
AGE-INDUCED
INTERFERON
RESPONSE
.
55
4.6.
AGING
INDUCES
A
GENERAL
OPEN
CHROMATIN
REARRANGEMENT
WHICH
IS
NOT
RESCUED
BY
DR,
EXCEPT
FOR
STATL
AND
IRF
TARGET
GENES
.
64
4.7.
GUT
BACTERIAL
LOAD
AND
COMPOSITION
DEPENDS
ON
AGE,
INTESTINAL
PART
AND
GENDER
.
78
4.8.
GUT
MICROBIOME
DYSBIOSIS
IS
ASSOCIATED
WITH
THE
INFLAMMAGING
.
90
5.
DISCUSSION
.
102
6.
REFERENCE
.
112 |
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| spelling | Rasa, Seyed Mohammad Mahdi 1989- Verfasser (DE-588)1241283192 aut Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa Jena 2021 126 Seiten Illustrationen, Diagramme 29,5 cm txt rdacontent n rdamedia nc rdacarrier Dissertation Friedrich-Schiller-Universität Jena 2021 Altern (DE-588)4068596-2 gnd rswk-swf Transkriptom (DE-588)4775955-0 gnd rswk-swf Mikrobiom Genetik (DE-588)1111812888 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Altern (DE-588)4068596-2 s Transkriptom (DE-588)4775955-0 s Mikrobiom Genetik (DE-588)1111812888 s DE-604 B:DE-101 application/pdf https://d-nb.info/1247774708/04 Inhaltsverzeichnis DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033266516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rasa, Seyed Mohammad Mahdi 1989- Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging Altern (DE-588)4068596-2 gnd Transkriptom (DE-588)4775955-0 gnd Mikrobiom Genetik (DE-588)1111812888 gnd |
| subject_GND | (DE-588)4068596-2 (DE-588)4775955-0 (DE-588)1111812888 (DE-588)4113937-9 |
| title | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_auth | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_exact_search | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_exact_search_txtP | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_full | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa |
| title_fullStr | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa |
| title_full_unstemmed | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging by M. Sc., Seyed Mohammad Mahdi Rasa |
| title_short | Characterization of the transcriptome, the epigenome, and the microbiome during aging: dietary restriction ameliorates inflammaging |
| title_sort | characterization of the transcriptome the epigenome and the microbiome during aging dietary restriction ameliorates inflammaging |
| topic | Altern (DE-588)4068596-2 gnd Transkriptom (DE-588)4775955-0 gnd Mikrobiom Genetik (DE-588)1111812888 gnd |
| topic_facet | Altern Transkriptom Mikrobiom Genetik Hochschulschrift |
| url | https://d-nb.info/1247774708/04 http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033266516&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rasaseyedmohammadmahdi characterizationofthetranscriptometheepigenomeandthemicrobiomeduringagingdietaryrestrictionamelioratesinflammaging |
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