Cell-free gene expression: methods and protocols
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparatio...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2022]
|
| Schriftenreihe: | Methods in molecular biology
2433 |
| Schlagworte: | |
| Zusammenfassung: | This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications |
| Beschreibung: | xv, 435 Seiten Illustrationen, Diagramme 1034 grams |
| ISBN: | 9781071619971 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV047697334 | ||
| 003 | DE-604 | ||
| 005 | 20221012 | ||
| 007 | t | ||
| 008 | 220119s2022 a||| |||| 00||| eng d | ||
| 020 | |a 9781071619971 |c hbk. |9 978-1-0716-1997-1 | ||
| 024 | 3 | |a 9781071619971 | |
| 035 | |a (OCoLC)1312694203 | ||
| 035 | |a (DE-599)BVBBV047697334 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-29T |a DE-19 | ||
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 245 | 1 | 0 | |a Cell-free gene expression |b methods and protocols |c edited by Ashty S. Karim, Michael C. Jewett |
| 264 | 1 | |a New York, NY |b Humana Press |c [2022] | |
| 300 | |a xv, 435 Seiten |b Illustrationen, Diagramme |c 1034 grams | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 2433 | |
| 490 | 0 | |a Springer protocols | |
| 520 | |a This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications | ||
| 650 | 4 | |a Biology—Technique | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a Biotechnology | |
| 650 | 4 | |a Synthetic Biology | |
| 650 | 0 | 7 | |a Genexpression |0 (DE-588)4020136-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a In vitro |0 (DE-588)4250701-7 |2 gnd |9 rswk-swf |
| 653 | |a Hardcover, Softcover / Biologie/Genetik, Gentechnik | ||
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Genexpression |0 (DE-588)4020136-3 |D s |
| 689 | 0 | 1 | |a In vitro |0 (DE-588)4250701-7 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Karim, Ashty S. |4 edt | |
| 700 | 1 | |a Jewett, Michael C. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 978-1-0716-1998-8 |
| 830 | 0 | |a Methods in molecular biology |v 2433 |w (DE-604)BV035362695 |9 2433 | |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-033081305 | ||
Datensatz im Suchindex
| _version_ | 1804183185201823744 |
|---|---|
| adam_txt | |
| any_adam_object | |
| any_adam_object_boolean | |
| author2 | Karim, Ashty S. Jewett, Michael C. |
| author2_role | edt edt |
| author2_variant | a s k as ask m c j mc mcj |
| author_facet | Karim, Ashty S. Jewett, Michael C. |
| building | Verbundindex |
| bvnumber | BV047697334 |
| classification_rvk | WC 4460 |
| ctrlnum | (OCoLC)1312694203 (DE-599)BVBBV047697334 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02724nam a2200481 cb4500</leader><controlfield tag="001">BV047697334</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20221012 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">220119s2022 a||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781071619971</subfield><subfield code="c">hbk.</subfield><subfield code="9">978-1-0716-1997-1</subfield></datafield><datafield tag="024" ind1="3" ind2=" "><subfield code="a">9781071619971</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1312694203</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV047697334</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-29T</subfield><subfield code="a">DE-19</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cell-free gene expression</subfield><subfield code="b">methods and protocols</subfield><subfield code="c">edited by Ashty S. Karim, Michael C. Jewett</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Humana Press</subfield><subfield code="c">[2022]</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">xv, 435 Seiten</subfield><subfield code="b">Illustrationen, Diagramme</subfield><subfield code="c">1034 grams</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">2433</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Springer protocols</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biology—Technique</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene expression</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biotechnology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Synthetic Biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genexpression</subfield><subfield code="0">(DE-588)4020136-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">In vitro</subfield><subfield code="0">(DE-588)4250701-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="653" ind1=" " ind2=" "><subfield code="a">Hardcover, Softcover / Biologie/Genetik, Gentechnik</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Genexpression</subfield><subfield code="0">(DE-588)4020136-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">In vitro</subfield><subfield code="0">(DE-588)4250701-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Karim, Ashty S.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jewett, Michael C.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Online-Ausgabe</subfield><subfield code="z">978-1-0716-1998-8</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">2433</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">2433</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-033081305</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV047697334 |
| illustrated | Illustrated |
| index_date | 2024-07-03T18:58:02Z |
| indexdate | 2024-07-10T09:19:25Z |
| institution | BVB |
| isbn | 9781071619971 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033081305 |
| oclc_num | 1312694203 |
| open_access_boolean | |
| owner | DE-29T DE-19 DE-BY-UBM |
| owner_facet | DE-29T DE-19 DE-BY-UBM |
| physical | xv, 435 Seiten Illustrationen, Diagramme 1034 grams |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology Springer protocols |
| spelling | Cell-free gene expression methods and protocols edited by Ashty S. Karim, Michael C. Jewett New York, NY Humana Press [2022] xv, 435 Seiten Illustrationen, Diagramme 1034 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2433 Springer protocols This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications Biology—Technique Gene expression Biotechnology Synthetic Biology Genexpression (DE-588)4020136-3 gnd rswk-swf In vitro (DE-588)4250701-7 gnd rswk-swf Hardcover, Softcover / Biologie/Genetik, Gentechnik (DE-588)4143413-4 Aufsatzsammlung gnd-content Genexpression (DE-588)4020136-3 s In vitro (DE-588)4250701-7 s DE-604 Karim, Ashty S. edt Jewett, Michael C. edt Erscheint auch als Online-Ausgabe 978-1-0716-1998-8 Methods in molecular biology 2433 (DE-604)BV035362695 2433 |
| spellingShingle | Cell-free gene expression methods and protocols Methods in molecular biology Biology—Technique Gene expression Biotechnology Synthetic Biology Genexpression (DE-588)4020136-3 gnd In vitro (DE-588)4250701-7 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4250701-7 (DE-588)4143413-4 |
| title | Cell-free gene expression methods and protocols |
| title_auth | Cell-free gene expression methods and protocols |
| title_exact_search | Cell-free gene expression methods and protocols |
| title_exact_search_txtP | Cell-free gene expression methods and protocols |
| title_full | Cell-free gene expression methods and protocols edited by Ashty S. Karim, Michael C. Jewett |
| title_fullStr | Cell-free gene expression methods and protocols edited by Ashty S. Karim, Michael C. Jewett |
| title_full_unstemmed | Cell-free gene expression methods and protocols edited by Ashty S. Karim, Michael C. Jewett |
| title_short | Cell-free gene expression |
| title_sort | cell free gene expression methods and protocols |
| title_sub | methods and protocols |
| topic | Biology—Technique Gene expression Biotechnology Synthetic Biology Genexpression (DE-588)4020136-3 gnd In vitro (DE-588)4250701-7 gnd |
| topic_facet | Biology—Technique Gene expression Biotechnology Synthetic Biology Genexpression In vitro Aufsatzsammlung |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT karimashtys cellfreegeneexpressionmethodsandprotocols AT jewettmichaelc cellfreegeneexpressionmethodsandprotocols |