New methods and sensors for membrane and cell volume research:
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Cambridge, MA ; San Diego, CA ; Oxford, United Kingdom ; London, United Kingdom
Academic Press, an imprint of Elsevier
2021
|
| Ausgabe: | First edition |
| Schriftenreihe: | Current topics in membranes
volume 88 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | xiv, 412 Seiten Illustrationen, Diagramme |
| ISBN: | 9780323911146 |
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| 245 | 1 | 0 | |a New methods and sensors for membrane and cell volume research |c edited by Michael A. Model (Kent State University, Kent, Ohio, United States), Irena Levitan (Division of Pulmonary and Critical Care, Departement of Medicine, University of Illinois at Chicago, Chicago, IL, United States) |
| 250 | |a First edition | ||
| 264 | 1 | |a Cambridge, MA ; San Diego, CA ; Oxford, United Kingdom ; London, United Kingdom |b Academic Press, an imprint of Elsevier |c 2021 | |
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Datensatz im Suchindex
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| adam_text | Contents Contributors ix On, in, and under membrane xi 1. Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell 1 Luca Mantovanelli, Bauke F. Gaastra, and Bert Poolman Ί. Introduction 2 2. Currently available fluorescent molecules 5 3. Labeling of (macro)molecules of interest 15 4. Sensors and methods of detection 18 5. Tracking of molecular and global changes 24 6. Microscopy techniques 36 7. A map to navigate the fluorescent sea 41 8. Conclusions 43 Acknowledgments 43 References 43 2. Current methods for studying intracellular liquid-liquid phase separation 55 Amber R. Titus and Edgar E. Kooijman 1. Characteristics of liquid-liquid phase separation 55 2. Liquid-liquid phase separation In biology 57 3. In vivo and in vitro methods of liquid-liquid phase separation detection 61 4. Computational methods for liquid-liquid phase separation prediction and modeling 65 5. Databases on liquid-liquid phase separation and Intrinsically disordered proteins 66 6. Summary 68 Acknowledgments 68 References 68 3. Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET 75 Sarah Lecinski, Jack W. Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald, and Mark C. Leake 1. Introduction 76 2. Results and discussion 87 v
Contents VI 3. Conclusion and discussion 100 4. Materials and methods 104 Acknowledgments 108 Funding sources 108 References 108 4. The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC 119 Yulia Kolobkova, Sumaira Pervaiz, and Tobias Stauber 1. Introduction 120 2. Animal models reveal the physiological functions of VRAC 122 3. Electrophysiologlcal measurement of VRAC-mediated currents 124 4. Measurement of transported substrate 142 5. Measurement of cell volume 146 6. Monitoring VRAC activity by optical imaging 147 References 149 5. Studying cell volume beyond cell volume 165 Michael A. Model 1. Introduction 166 2. CV measurement by dye exclusion 169 3. MC measurement by TIE/1TD 173 4. MC at work 174 5. Conclusions 181 Acknowledgments 182 Appendix 182 References 185 6. Membrane tension 189 Pei-Chuan Chao and Frederick Sachs 7. Methods for assessment of membrane protrusion dynamics 205 Jordan Fauser, Martin Brennan, Denis Tsygankov, and Andrei V. Karginov 1. Introduction 206 2. Approaches to assess physical changes in cell morphodynamics 208 3. Approaches to theoretical modeling of cell protrusion activity 221 4. Biochemical assessment of membrane protrusion dynamics 224 5. Conclusion 230 Acknowledgment 231 References 231
Contents vii 8. Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids 235 Irena Levitan 1. Introduction 236 2. Basic principles of Laurdan fluorescence 236 3. Laurdan two photon imaging: Visualizing domains in membrane vesicles 239 4. Laurdan two photon imaging: Visualizing membrane domains in living cells 5. Impact of cholesterol depletion on Laurdan GP values 241 243 6. Opposite effects of low-density lipoproteins (LDL) and oxidized low-density lipoproteins (oxLDL) on the lipid order of endothelial cells 247 7. Disruption of lipid packing of endothelial membrane by oxidized lipids: Oxysterols and oxidized phospholipids 249 8. Challenges and new directions 252 Acknowledgments 253 References 253 9. Fluorescence sensors for imaging membrane lipid domains and cholesterol 257 Francisco J. Barrantes 1. Introduction 258 2. Fluorescent sensors for imaging lipid domains in situ 262 3. Sensing transbilayer topography of lipids, cholesterol, and lipid domains 270 4. Sensing membrane polarity 283 5. Super-resolution microscopy triggers the development of new sensors 288 6. Conclusions and future directions 294 Acknowledgments 296 Author contributions 297 Declaration of interests 297 References 297 10. Mass spectrometry-based lipid analysis and imaging 315 Koralege C. Pathmasiri, Thu T.A. Nguyen, Nigina Khamidova, and Stephanie M. Cologna 1. Introduction to matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) 316 2. MALDI MSI lipid imaging workflow 323 3. MALDI MSI data analysis 339 4. Lipid identification in MSİ
341 5. Applications of MALDI MSI in Niemann-Pick type C 342 6. Current perspective 343 References 344
viii Contents 11. Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering 359 Ursula Perez-Salas, Sumit Garg, Yuri Gerelli, and Lionel Porcar 1. Introduction 360 2. Small angle neutron scattering (SANS) in the study of membranes 362 3. Kinetic and thermodynamic characteristics of the transport of lipids and sterols between and within membranes obtained from TR-SANS measurements 375 4. Transport behavior of lipids and sterols in membranes 381 5. Current and future perspectives 400 Acknowledgments 402 References 402
|
| adam_txt |
Contents Contributors ix On, in, and under membrane xi 1. Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell 1 Luca Mantovanelli, Bauke F. Gaastra, and Bert Poolman Ί. Introduction 2 2. Currently available fluorescent molecules 5 3. Labeling of (macro)molecules of interest 15 4. Sensors and methods of detection 18 5. Tracking of molecular and global changes 24 6. Microscopy techniques 36 7. A map to navigate the fluorescent sea 41 8. Conclusions 43 Acknowledgments 43 References 43 2. Current methods for studying intracellular liquid-liquid phase separation 55 Amber R. Titus and Edgar E. Kooijman 1. Characteristics of liquid-liquid phase separation 55 2. Liquid-liquid phase separation In biology 57 3. In vivo and in vitro methods of liquid-liquid phase separation detection 61 4. Computational methods for liquid-liquid phase separation prediction and modeling 65 5. Databases on liquid-liquid phase separation and Intrinsically disordered proteins 66 6. Summary 68 Acknowledgments 68 References 68 3. Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET 75 Sarah Lecinski, Jack W. Shepherd, Lewis Frame, Imogen Hayton, Chris MacDonald, and Mark C. Leake 1. Introduction 76 2. Results and discussion 87 v
Contents VI 3. Conclusion and discussion 100 4. Materials and methods 104 Acknowledgments 108 Funding sources 108 References 108 4. The expanding toolbox to study the LRRC8-formed volume-regulated anion channel VRAC 119 Yulia Kolobkova, Sumaira Pervaiz, and Tobias Stauber 1. Introduction 120 2. Animal models reveal the physiological functions of VRAC 122 3. Electrophysiologlcal measurement of VRAC-mediated currents 124 4. Measurement of transported substrate 142 5. Measurement of cell volume 146 6. Monitoring VRAC activity by optical imaging 147 References 149 5. Studying cell volume beyond cell volume 165 Michael A. Model 1. Introduction 166 2. CV measurement by dye exclusion 169 3. MC measurement by TIE/1TD 173 4. MC at work 174 5. Conclusions 181 Acknowledgments 182 Appendix 182 References 185 6. Membrane tension 189 Pei-Chuan Chao and Frederick Sachs 7. Methods for assessment of membrane protrusion dynamics 205 Jordan Fauser, Martin Brennan, Denis Tsygankov, and Andrei V. Karginov 1. Introduction 206 2. Approaches to assess physical changes in cell morphodynamics 208 3. Approaches to theoretical modeling of cell protrusion activity 221 4. Biochemical assessment of membrane protrusion dynamics 224 5. Conclusion 230 Acknowledgment 231 References 231
Contents vii 8. Evaluating membrane structure by Laurdan imaging: Disruption of lipid packing by oxidized lipids 235 Irena Levitan 1. Introduction 236 2. Basic principles of Laurdan fluorescence 236 3. Laurdan two photon imaging: Visualizing domains in membrane vesicles 239 4. Laurdan two photon imaging: Visualizing membrane domains in living cells 5. Impact of cholesterol depletion on Laurdan GP values 241 243 6. Opposite effects of low-density lipoproteins (LDL) and oxidized low-density lipoproteins (oxLDL) on the lipid order of endothelial cells 247 7. Disruption of lipid packing of endothelial membrane by oxidized lipids: Oxysterols and oxidized phospholipids 249 8. Challenges and new directions 252 Acknowledgments 253 References 253 9. Fluorescence sensors for imaging membrane lipid domains and cholesterol 257 Francisco J. Barrantes 1. Introduction 258 2. Fluorescent sensors for imaging lipid domains in situ 262 3. Sensing transbilayer topography of lipids, cholesterol, and lipid domains 270 4. Sensing membrane polarity 283 5. Super-resolution microscopy triggers the development of new sensors 288 6. Conclusions and future directions 294 Acknowledgments 296 Author contributions 297 Declaration of interests 297 References 297 10. Mass spectrometry-based lipid analysis and imaging 315 Koralege C. Pathmasiri, Thu T.A. Nguyen, Nigina Khamidova, and Stephanie M. Cologna 1. Introduction to matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) 316 2. MALDI MSI lipid imaging workflow 323 3. MALDI MSI data analysis 339 4. Lipid identification in MSİ
341 5. Applications of MALDI MSI in Niemann-Pick type C 342 6. Current perspective 343 References 344
viii Contents 11. Deciphering lipid transfer between and within membranes with time-resolved small-angle neutron scattering 359 Ursula Perez-Salas, Sumit Garg, Yuri Gerelli, and Lionel Porcar 1. Introduction 360 2. Small angle neutron scattering (SANS) in the study of membranes 362 3. Kinetic and thermodynamic characteristics of the transport of lipids and sterols between and within membranes obtained from TR-SANS measurements 375 4. Transport behavior of lipids and sterols in membranes 381 5. Current and future perspectives 400 Acknowledgments 402 References 402 |
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| spelling | New methods and sensors for membrane and cell volume research edited by Michael A. Model (Kent State University, Kent, Ohio, United States), Irena Levitan (Division of Pulmonary and Critical Care, Departement of Medicine, University of Illinois at Chicago, Chicago, IL, United States) First edition Cambridge, MA ; San Diego, CA ; Oxford, United Kingdom ; London, United Kingdom Academic Press, an imprint of Elsevier 2021 xiv, 412 Seiten Illustrationen, Diagramme txt rdacontent n rdamedia nc rdacarrier Current topics in membranes volume 88 Cytosol (DE-588)4285082-4 gnd rswk-swf Plasmamembran (DE-588)4067550-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Plasmamembran (DE-588)4067550-6 s Cytosol (DE-588)4285082-4 s DE-604 Model, Michael A. edt Levitan, Irena (DE-588)1170911080 edt Current topics in membranes volume 88 (DE-604)BV036839511 88 Digitalisierung UB Regensburg - ADAM Catalogue Enrichment application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=033073721&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
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| title | New methods and sensors for membrane and cell volume research |
| title_auth | New methods and sensors for membrane and cell volume research |
| title_exact_search | New methods and sensors for membrane and cell volume research |
| title_exact_search_txtP | New methods and sensors for membrane and cell volume research |
| title_full | New methods and sensors for membrane and cell volume research edited by Michael A. Model (Kent State University, Kent, Ohio, United States), Irena Levitan (Division of Pulmonary and Critical Care, Departement of Medicine, University of Illinois at Chicago, Chicago, IL, United States) |
| title_fullStr | New methods and sensors for membrane and cell volume research edited by Michael A. Model (Kent State University, Kent, Ohio, United States), Irena Levitan (Division of Pulmonary and Critical Care, Departement of Medicine, University of Illinois at Chicago, Chicago, IL, United States) |
| title_full_unstemmed | New methods and sensors for membrane and cell volume research edited by Michael A. Model (Kent State University, Kent, Ohio, United States), Irena Levitan (Division of Pulmonary and Critical Care, Departement of Medicine, University of Illinois at Chicago, Chicago, IL, United States) |
| title_short | New methods and sensors for membrane and cell volume research |
| title_sort | new methods and sensors for membrane and cell volume research |
| topic | Cytosol (DE-588)4285082-4 gnd Plasmamembran (DE-588)4067550-6 gnd |
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