Membrane lipids: methods and protocols
This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with el...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Humana Press
[2022]
|
| Schriftenreihe: | Methods in molecular biology
2402 |
| Schlagworte: | |
| Zusammenfassung: | This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics |
| Beschreibung: | 1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles; Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang; ; 2. Preparing Ion Channel Switch Membrane-Based Biosensors; Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela; ; 3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer; Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt; ; 4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes; Tadas Penkauskas, Filipas Ambrulevicius, and Gintaras Valincius; ; 5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes; Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster; ; 6. - Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs); Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster; ; 7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes; Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield; ; 8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer; Kan Shoji; ; 9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations; Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes; ; 10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations; Robby Manrique; ; 11. - Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins; Amanda Buyan and Ben Corry; ; 12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling; Kathleen Wood; ; 13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation; Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt; ; 14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering; Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt; ; 15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers; Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson; ; 16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs); Kinga Burdach, Damian Dziubak, and Slawomir Sek; ; 17. - Manipulation of Lipid Membranes with Thermal Stimuli; Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen; ; 18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid ß Oligomers by Atomic Force Microscopy; Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta; ; 19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains; Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté; ; 20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR; Elvis Pandzic, Renee Whan, and Alex Macmillan; ; 21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides; Srikanth Dumpati and Debarun Dutta; ; 22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi; Maria Makarova and Dylan M. Owen |
| Beschreibung: | xi, 300 Seiten Illustrationen, Diagramme 774 grams |
| ISBN: | 9781071618424 |
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| 500 | |a - Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs); Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster; ; 7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes; Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield; ; 8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer; Kan Shoji; ; 9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations; Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes; ; 10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations; Robby Manrique; ; 11. | ||
| 500 | |a - Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins; Amanda Buyan and Ben Corry; ; 12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling; Kathleen Wood; ; 13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation; Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt; ; 14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering; Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt; ; 15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers; Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson; ; 16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs); Kinga Burdach, Damian Dziubak, and Slawomir Sek; ; 17. | ||
| 500 | |a - Manipulation of Lipid Membranes with Thermal Stimuli; Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen; ; 18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid ß Oligomers by Atomic Force Microscopy; Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta; ; 19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains; Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté; ; 20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR; Elvis Pandzic, Renee Whan, and Alex Macmillan; ; 21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides; Srikanth Dumpati and Debarun Dutta; ; 22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi; Maria Makarova and Dylan M. Owen | ||
| 520 | |a This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics | ||
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| id | DE-604.BV047659969 |
| illustrated | Illustrated |
| index_date | 2024-07-03T18:52:15Z |
| indexdate | 2024-07-10T09:18:33Z |
| institution | BVB |
| isbn | 9781071618424 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-033044808 |
| oclc_num | 1296305650 |
| open_access_boolean | |
| owner | DE-29T |
| owner_facet | DE-29T |
| physical | xi, 300 Seiten Illustrationen, Diagramme 774 grams |
| publishDate | 2022 |
| publishDateSearch | 2022 |
| publishDateSort | 2022 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Membrane lipids methods and protocols edited by Charles G. Cranfield (School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia) New York, NY Humana Press [2022] xi, 300 Seiten Illustrationen, Diagramme 774 grams txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 2402 1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles; Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang; ; 2. Preparing Ion Channel Switch Membrane-Based Biosensors; Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela; ; 3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer; Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt; ; 4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes; Tadas Penkauskas, Filipas Ambrulevicius, and Gintaras Valincius; ; 5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes; Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster; ; 6. - Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs); Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster; ; 7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes; Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield; ; 8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer; Kan Shoji; ; 9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations; Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes; ; 10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations; Robby Manrique; ; 11. - Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins; Amanda Buyan and Ben Corry; ; 12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling; Kathleen Wood; ; 13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation; Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt; ; 14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering; Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt; ; 15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers; Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson; ; 16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs); Kinga Burdach, Damian Dziubak, and Slawomir Sek; ; 17. - Manipulation of Lipid Membranes with Thermal Stimuli; Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen; ; 18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid ß Oligomers by Atomic Force Microscopy; Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta; ; 19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains; Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté; ; 20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR; Elvis Pandzic, Renee Whan, and Alex Macmillan; ; 21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides; Srikanth Dumpati and Debarun Dutta; ; 22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi; Maria Makarova and Dylan M. Owen This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics bicssc bisacsh Cell biology Biophysics Biological physics Lipids Life sciences Lipidmembran (DE-588)4167790-0 gnd rswk-swf Molekularbiologische Methode (DE-588)7650063-9 gnd rswk-swf Hardcover, Softcover / Biologie/Mikrobiologie Lipidmembran (DE-588)4167790-0 s Molekularbiologische Methode (DE-588)7650063-9 s DE-604 Cranfield, Charles G. edt Erscheint auch als Online-Ausgabe 978-1-07-161843-1 Methods in molecular biology 2402 (DE-604)BV035362695 2402 |
| spellingShingle | Membrane lipids methods and protocols Methods in molecular biology bicssc bisacsh Cell biology Biophysics Biological physics Lipids Life sciences Lipidmembran (DE-588)4167790-0 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| subject_GND | (DE-588)4167790-0 (DE-588)7650063-9 |
| title | Membrane lipids methods and protocols |
| title_auth | Membrane lipids methods and protocols |
| title_exact_search | Membrane lipids methods and protocols |
| title_exact_search_txtP | Membrane lipids methods and protocols |
| title_full | Membrane lipids methods and protocols edited by Charles G. Cranfield (School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia) |
| title_fullStr | Membrane lipids methods and protocols edited by Charles G. Cranfield (School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia) |
| title_full_unstemmed | Membrane lipids methods and protocols edited by Charles G. Cranfield (School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia) |
| title_short | Membrane lipids |
| title_sort | membrane lipids methods and protocols |
| title_sub | methods and protocols |
| topic | bicssc bisacsh Cell biology Biophysics Biological physics Lipids Life sciences Lipidmembran (DE-588)4167790-0 gnd Molekularbiologische Methode (DE-588)7650063-9 gnd |
| topic_facet | bicssc bisacsh Cell biology Biophysics Biological physics Lipids Life sciences Lipidmembran Molekularbiologische Methode |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT cranfieldcharlesg membranelipidsmethodsandprotocols |