Organoids: stem cells, structure, and function
This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating t...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1576 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research |
| Beschreibung: | A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- - New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- - Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures |
| Beschreibung: | 1 Online-Ressource (XV, 363 Seiten) Illustrationen |
| ISBN: | 9781493976171 |
| DOI: | 10.1007/978-1-4939-7617-1 |
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| isbn | 9781493976171 |
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| spelling | Organoids stem cells, structure, and function edited by Kursad Turksen 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XV, 363 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1576 A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells -- Construction of Thymus Organoids from Decellularized Thymus Scaffolds -- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres -- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells -- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix -- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension -- Efficient Culture of Intestinal Organoids with Blebbistatin -- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion -- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine -- - New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice -- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System -- Murine Colonic Organoid Culture System and Downstream Assay Applications -- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions -- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism -- Oncogenic Transformation of Human-Derived Gastric Organoids -- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation -- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections -- Study Bacterial-Host Interactions Using Intestinal Organoids -- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering -- - Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo -- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells -- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids -- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly -- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying -- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues -- Drug Sensitivity Assays of Human Cancer Organoid Cultures This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research Cell biology Turksen, Kursad ca. 20./21. Jh. (DE-588)1077175922 edt Erscheint auch als Druck-Ausgabe 978-1-4939-7618-8 https://doi.org/10.1007/978-1-4939-7617-1 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Organoids stem cells, structure, and function Cell biology |
| title | Organoids stem cells, structure, and function |
| title_auth | Organoids stem cells, structure, and function |
| title_exact_search | Organoids stem cells, structure, and function |
| title_exact_search_txtP | Organoids stem cells, structure, and function |
| title_full | Organoids stem cells, structure, and function edited by Kursad Turksen |
| title_fullStr | Organoids stem cells, structure, and function edited by Kursad Turksen |
| title_full_unstemmed | Organoids stem cells, structure, and function edited by Kursad Turksen |
| title_short | Organoids |
| title_sort | organoids stem cells structure and function |
| title_sub | stem cells, structure, and function |
| topic | Cell biology |
| topic_facet | Cell biology |
| url | https://doi.org/10.1007/978-1-4939-7617-1 |
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