The ESCRT complexes: methods and protocols
This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalia...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1998 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology |
| Beschreibung: | Analysis of the Archaeal ESCRT Apparatus -- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster -- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc -- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy -- Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells -- Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy -- Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking -- Genetic and Biochemical Analyses of Yeast ESCRT -- Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons -- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT -- - Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software -- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts -- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins -- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro -- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays -- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting -- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production -- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast -- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis -- - Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana -- Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay -- RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells |
| Beschreibung: | 1 Online-Ressource (XIV, 322 Seiten) Illustrationen |
| ISBN: | 9781493994922 |
| DOI: | 10.1007/978-1-4939-9492-2 |
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| 500 | |a - Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software -- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts -- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins -- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro -- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays -- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting -- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production -- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast -- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis -- | ||
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| 520 | |a This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology | ||
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Datensatz im Suchindex
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| discipline | Biologie |
| discipline_str_mv | Biologie |
| doi_str_mv | 10.1007/978-1-4939-9492-2 |
| edition | 1st ed. 2019 |
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| spelling | The ESCRT complexes methods and protocols edited by Emmanuel Culetto, Renaud Legouis 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XIV, 322 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1998 Analysis of the Archaeal ESCRT Apparatus -- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster -- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc -- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy -- Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells -- Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy -- Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking -- Genetic and Biochemical Analyses of Yeast ESCRT -- Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons -- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT -- - Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software -- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts -- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins -- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro -- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays -- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting -- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production -- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast -- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis -- - Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana -- Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay -- RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology Cell membranes Proteins Cell physiology Culetto, Emmanuel (DE-588)1191200299 edt Legouis, Renaud (DE-588)1191200469 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9494-6 https://doi.org/10.1007/978-1-4939-9492-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | The ESCRT complexes methods and protocols Cell membranes Proteins Cell physiology |
| title | The ESCRT complexes methods and protocols |
| title_auth | The ESCRT complexes methods and protocols |
| title_exact_search | The ESCRT complexes methods and protocols |
| title_exact_search_txtP | The ESCRT complexes methods and protocols |
| title_full | The ESCRT complexes methods and protocols edited by Emmanuel Culetto, Renaud Legouis |
| title_fullStr | The ESCRT complexes methods and protocols edited by Emmanuel Culetto, Renaud Legouis |
| title_full_unstemmed | The ESCRT complexes methods and protocols edited by Emmanuel Culetto, Renaud Legouis |
| title_short | The ESCRT complexes |
| title_sort | the escrt complexes methods and protocols |
| title_sub | methods and protocols |
| topic | Cell membranes Proteins Cell physiology |
| topic_facet | Cell membranes Proteins Cell physiology |
| url | https://doi.org/10.1007/978-1-4939-9492-2 |
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