Plant cell morphogenesis: methods and protocols
This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell struct...
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Weitere Verfasser: | , |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
New York, NY
Springer New York
2019
|
Ausgabe: | 2nd ed. 2019 |
Schriftenreihe: | Methods in Molecular Biology
1992 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs |
Beschreibung: | 1. Essential Methods of Plant Sample Preparation for Light Microscopy -- 2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy -- 3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections -- 4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature -- 5. Fluorescence Lifetime Imaging of Plant Cell Walls -- 6. Raman Spectroscopy in Non-Woody Plants -- 7. Image Analysis: Basic Procedures for Description of Plant Structures -- 8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing -- 9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics -- 10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D -- 11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions -- - 12. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells -- 13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics -- 14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells -- 15. Optical Trapping in Plant Cells -- 16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation -- 17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope -- 18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX -- 19. Kinematic Characterization of Root Growth by Means of Stripflow -- 20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens -- 21. Live Cell Imaging of Arabidopsis Root Hairs -- 22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant -- - 23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls -- 24. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes -- 25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to ‑Omics |
Beschreibung: | 1 Online-Ressource (XI, 380 Seiten) Illustrationen |
ISBN: | 9781493994694 |
DOI: | 10.1007/978-1-4939-9469-4 |
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500 | |a - 12. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells -- 13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics -- 14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells -- 15. Optical Trapping in Plant Cells -- 16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation -- 17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope -- 18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX -- 19. Kinematic Characterization of Root Growth by Means of Stripflow -- 20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens -- 21. Live Cell Imaging of Arabidopsis Root Hairs -- 22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant -- | ||
500 | |a - 23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls -- 24. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes -- 25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to ‑Omics | ||
520 | |a This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs | ||
650 | 4 | |a Plant physiology | |
650 | 4 | |a Plant science | |
650 | 4 | |a Botany | |
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author_facet | Cvrčková, Fatima 1966- Žárský, Viktor 1958- |
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dewey-ones | 571 - Physiology & related subjects |
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discipline | Biologie |
discipline_str_mv | Biologie |
doi_str_mv | 10.1007/978-1-4939-9469-4 |
edition | 2nd ed. 2019 |
format | Electronic eBook |
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spelling | Plant cell morphogenesis methods and protocols edited by Fatima Cvrčková, Viktor Žárský 2nd ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 380 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1992 1. Essential Methods of Plant Sample Preparation for Light Microscopy -- 2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy -- 3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections -- 4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature -- 5. Fluorescence Lifetime Imaging of Plant Cell Walls -- 6. Raman Spectroscopy in Non-Woody Plants -- 7. Image Analysis: Basic Procedures for Description of Plant Structures -- 8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing -- 9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics -- 10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D -- 11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions -- - 12. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells -- 13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics -- 14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells -- 15. Optical Trapping in Plant Cells -- 16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation -- 17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope -- 18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX -- 19. Kinematic Characterization of Root Growth by Means of Stripflow -- 20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens -- 21. Live Cell Imaging of Arabidopsis Root Hairs -- 22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant -- - 23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls -- 24. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes -- 25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to ‑Omics This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs Plant physiology Plant science Botany Cvrčková, Fatima 1966- (DE-588)1247858375 edt Žárský, Viktor 1958- (DE-588)1048304442 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9471-7 https://doi.org/10.1007/978-1-4939-9469-4 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Plant cell morphogenesis methods and protocols Plant physiology Plant science Botany |
title | Plant cell morphogenesis methods and protocols |
title_auth | Plant cell morphogenesis methods and protocols |
title_exact_search | Plant cell morphogenesis methods and protocols |
title_exact_search_txtP | Plant cell morphogenesis methods and protocols |
title_full | Plant cell morphogenesis methods and protocols edited by Fatima Cvrčková, Viktor Žárský |
title_fullStr | Plant cell morphogenesis methods and protocols edited by Fatima Cvrčková, Viktor Žárský |
title_full_unstemmed | Plant cell morphogenesis methods and protocols edited by Fatima Cvrčková, Viktor Žárský |
title_short | Plant cell morphogenesis |
title_sort | plant cell morphogenesis methods and protocols |
title_sub | methods and protocols |
topic | Plant physiology Plant science Botany |
topic_facet | Plant physiology Plant science Botany |
url | https://doi.org/10.1007/978-1-4939-9469-4 |
work_keys_str_mv | AT cvrckovafatima plantcellmorphogenesismethodsandprotocols AT zarskyviktor plantcellmorphogenesismethodsandprotocols |