Cancer metabolism: methods and protocols
This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isoto...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1928 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories |
| Beschreibung: | Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction -- Imaging Cancer Metabolism with Positron Emission Tomography (PET) -- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells -- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics -- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) -- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells -- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment -- Quantitation of Macropinocytosis in Cancer Cells -- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation -- - Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- - Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism |
| Beschreibung: | 1 Online-Ressource (XI, 495 Seiten) Illustrationen |
| ISBN: | 9781493990276 |
| DOI: | 10.1007/978-1-4939-9027-6 |
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| 500 | |a - Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- | ||
| 500 | |a - Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism | ||
| 520 | |a This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories | ||
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| spelling | Cancer metabolism methods and protocols edited by Majda Haznadar 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 495 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1928 Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction -- Imaging Cancer Metabolism with Positron Emission Tomography (PET) -- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells -- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics -- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) -- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells -- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment -- Quantitation of Macropinocytosis in Cancer Cells -- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation -- - Methods to Measure Autophagy in Cancer Metabolism -- Lipidomic Analysis of Cancer Cell and Tumor Tissue -- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids -- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites -- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy -- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging -- Compositional Analysis of the Human Microbiome in Cancer Research -- Assessing Metabolic Dysregulation in Muscle During Cachexia -- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells -- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging -- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods -- Hyperpolarized MRI for Studying Tumor Metabolism -- - Overview of Glutamine-Dependency and Metabolic Rescue Protocols -- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype -- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways -- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism. The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories Cancer research Haznadar, Majda ca. 20./21. Jh. (DE-588)1247828697 edt Erscheint auch als Druck-Ausgabe 978-1-4939-9028-3 https://doi.org/10.1007/978-1-4939-9027-6 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Cancer metabolism methods and protocols Cancer research |
| title | Cancer metabolism methods and protocols |
| title_auth | Cancer metabolism methods and protocols |
| title_exact_search | Cancer metabolism methods and protocols |
| title_exact_search_txtP | Cancer metabolism methods and protocols |
| title_full | Cancer metabolism methods and protocols edited by Majda Haznadar |
| title_fullStr | Cancer metabolism methods and protocols edited by Majda Haznadar |
| title_full_unstemmed | Cancer metabolism methods and protocols edited by Majda Haznadar |
| title_short | Cancer metabolism |
| title_sort | cancer metabolism methods and protocols |
| title_sub | methods and protocols |
| topic | Cancer research |
| topic_facet | Cancer research |
| url | https://doi.org/10.1007/978-1-4939-9027-6 |
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