This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including...

Ausführliche Beschreibung

Bibliographische Detailangaben

Weitere Verfasser:	Yong, William H. (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	New York, NY Springer New York 2019
Ausgabe:	1st ed. 2019
Schriftenreihe:	Methods in Molecular Biology 1897
Schlagworte:	Laboratory medicine Medical microbiology Biochemistry
Online-Zugang:	UBR01 TUM01 URL des Erstveröffentlichers
Zusammenfassung:	This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies
Beschreibung:	Sustainability in Biobanking- An Introduction to Starting a Biobank -- An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks -- Disaster Prevention and Recovery -- Minority Participation in Biobanks: An Essential Key to Progress -- Orientation and Training of New Biobank Personnel -- Procurement and Storage of Surgical Biospecimens -- Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization -- Procurement, Storage, and Use of Blood in Biobanks -- Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens -- Biobanking of Cerebrospinal Fluid -- Biobanking of Urine Samples -- Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking -- The Fluid Preservation of Zoological Specimens -- Photographing Fluid Preserved Specimens -- Casts of Fluid Preserved Specimens -- Collection and Preservation of Terrestrial Arthropods -- Procurement and Preservation of Plants -- Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank -- Decontamination of Biobank Facilities -- Laboratory Safety: Chemical and Physical Hazards -- Making Formalin-Fixed Paraffin Embedded Blocks -- Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections -- Performing and Cutting Frozen Sections -- An Introduction to the Performance of Immunohistochemistry -- An Introduction to Performing Immunofluorescence Staining -- Making a Tissue Microarray -- Nucleic Acid Isolation and Quality Control -- Fundamentals of RNA Analysis on Biobanked Specimens -- Nucleic Acid Extraction from Human Biological Samples -- An Overview of DNA Analytical Methods -- Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs) -- Sample Preparation for Transmission Electron Microscopy -- Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines -- Domestic and International Shipping of Biospecimens.
Beschreibung:	1 Online-Ressource (XVI, 448 Seiten) Illustrationen
ISBN:	9781493989355
DOI:	10.1007/978-1-4939-8935-5