Hepatic stem cells: methods and protocols
This volume looks at the liver's epithelial cells-hepatocytes and cholangiocytes-and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstit...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2019
|
| Ausgabe: | 1st ed. 2019 |
| Schriftenreihe: | Methods in Molecular Biology
1905 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume looks at the liver's epithelial cells-hepatocytes and cholangiocytes-and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology |
| Beschreibung: | Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells -- Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver -- Identification and Isolation of Clonogenic Cholangiocyte in Mouse -- Isolation and Expansion of Rat Hepatocytic Progenitor Cells -- Genetic Lineage Tracing of Biliary Epithelial Cells -- Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination -- Analysis for the Heterogeneity of Liver Progenitor Cells -- Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration -- Conversion of Fibroblasts to Hepatocytes In Vitro -- Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo -- Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells -- Induction of Functional Hepatocytes from Human iPSCs -- Culture System of Bile Duct-Like Cystic Structures Derived from Human Inducible Pluripotent Stem Cells -- Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture -- Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device -- Generation of Hepatic Organoids with Biliary Structures -- Analysis for Remodeling Hepatic Tissue Structures in 3D during Regeneration -- Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells -- A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells -- Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes -- Mouse Model for Cholangiocarcinoma from Peribiliary Glands |
| Beschreibung: | 1 Online-Ressource (XI, 248 Seiten) Illustrationen |
| ISBN: | 9781493989614 |
| DOI: | 10.1007/978-1-4939-8961-4 |
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| 520 | |a This volume looks at the liver's epithelial cells-hepatocytes and cholangiocytes-and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology | ||
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| doi_str_mv | 10.1007/978-1-4939-8961-4 |
| edition | 1st ed. 2019 |
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| isbn | 9781493989614 |
| language | English |
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| spelling | Hepatic stem cells methods and protocols edited by Naoki Tanimizu 1st ed. 2019 New York, NY Springer New York 2019 1 Online-Ressource (XI, 248 Seiten) Illustrationen txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1905 Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells -- Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver -- Identification and Isolation of Clonogenic Cholangiocyte in Mouse -- Isolation and Expansion of Rat Hepatocytic Progenitor Cells -- Genetic Lineage Tracing of Biliary Epithelial Cells -- Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination -- Analysis for the Heterogeneity of Liver Progenitor Cells -- Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration -- Conversion of Fibroblasts to Hepatocytes In Vitro -- Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo -- Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells -- Induction of Functional Hepatocytes from Human iPSCs -- Culture System of Bile Duct-Like Cystic Structures Derived from Human Inducible Pluripotent Stem Cells -- Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture -- Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device -- Generation of Hepatic Organoids with Biliary Structures -- Analysis for Remodeling Hepatic Tissue Structures in 3D during Regeneration -- Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells -- A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells -- Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes -- Mouse Model for Cholangiocarcinoma from Peribiliary Glands This volume looks at the liver's epithelial cells-hepatocytes and cholangiocytes-and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology Stem cells Tanimizu, Naoki ca. 20./21. Jh. (DE-588)1247779300 edt Erscheint auch als Druck-Ausgabe 978-1-4939-8962-1 https://doi.org/10.1007/978-1-4939-8961-4 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Hepatic stem cells methods and protocols Stem cells |
| title | Hepatic stem cells methods and protocols |
| title_auth | Hepatic stem cells methods and protocols |
| title_exact_search | Hepatic stem cells methods and protocols |
| title_exact_search_txtP | Hepatic stem cells methods and protocols |
| title_full | Hepatic stem cells methods and protocols edited by Naoki Tanimizu |
| title_fullStr | Hepatic stem cells methods and protocols edited by Naoki Tanimizu |
| title_full_unstemmed | Hepatic stem cells methods and protocols edited by Naoki Tanimizu |
| title_short | Hepatic stem cells |
| title_sort | hepatic stem cells methods and protocols |
| title_sub | methods and protocols |
| topic | Stem cells |
| topic_facet | Stem cells |
| url | https://doi.org/10.1007/978-1-4939-8961-4 |
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